Abstract
The diogenid hermit crab genus Clibanarius Dana, 1852 is a group of decapod crustaceans commonly found in tropical or subtropical shallow waters. The species of the genus are usually characterized by distinct color patterns in life, but the identification of colorless specimens preserved in ethanol is sometimes very difficult. DNA barcoding can facilitate species identification and recognition. In this study, we determined partial sequences of the cytochrome oxidase I (COI) gene of 11 Clibanarius species commonly found in the intertidal zones of the Ryukyu Archipelago, southwestern Japan. The sequences can be used as nametags to distinguish each of the 11 species, as the data differ for each species. The COI gene sequences of Clibanarius show an AT bias, especially in the third codon position (A+T = 76.2%), as has been reported for some other anomuran species. Phylogenetic analyses based on the sequences also offer some suggestions on the interspecific relationships of Clibanarius.References
Ball, E.E. & Haig, J. (1972) Hermit crabs from eastern New Guinea. Pacific Science, 26, 87–107.
Buitendijk, A. (1937) Biological results of the Snellius Expedition. The Paguridae of the Snellius Expedition. Temminckia, 2, 251–280.
Dana, J.D. (1851) Conspectus crustaceorum quae in orbis terrarum circumnavigatione, Carolo Wilkes e classe reipublicae foederatae duce, lexit et descripsit. (Preprint from) Proceedings of the Academy of Natural Sciences, Philadelphia, 5, 267–272.
Dana, J.D. (1852) Conspectus crustaceorum, etc., Conspectus of the Crustacea of the Exploring Expedition under Capt. Wilkes, U.S.N., including the Paguridea, continued, the Megalopidea, and the Macroura. Paguridea, continued, and subtribe Megalopidea. (Preprint from) Proceedings of the Academy of Natural Sciences, Philadelphia, 6, 6–28.
De Haan, W. (1833–1850) Crustacea. Pp. i–xxxi, ix–xvi, 1–243. In: von Siebold, P.F. (Ed.), Fauna Japonica sive Descriptio Animalium, quae in Itinere per Japoniam, Jussu et Auspiciis Superiorum, qui Summum in India Batava Imperium Tenent, Suscepto, Annis 1823–1830 Collegit, Notis, Observationibus et Adumbrationibus Illustravit, Lugduni-Batavorum, Leiden.
De Man, J.G. (1888) Report on the Podophthalmous Crustacea of the Mergui Archipelago, collected for the Trustees of the Indian Museum, Calcutta, by Dr. John Anderson, F.R.S., Superintendent of the Museum, parts IV and V. Journal of the Linnean Society, London, 22, 177–240.
Folmer, O., Black, M., Hoeh, W., Lutz, R. & Vrijenhoek, R. (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3, 294–299.
Forest, J. (1953) Crustaces Decapodes Marcheurs des iles de Tahiti et des Tuamotu. I. Paguridea. Bulletin du Museum national d’Histoire naturelle, (2) 25, 441–450.
Forest, J. (1995) Crustacea Decapoda Anomura: Révision du genre Trizopagurus Forest, 1952 (Diogenidae) avec l’établissement de deux genres nouveaux. In: Crosnier, A. (Ed), Résultats des Campagnes MUSORSTOM, Volume 13. Mémoires du Muséum national d’Histoire naturelle, Paris, 163, 9–149.
Hickerson, M.J. & Cunningham, C.W. (2000) Dramatic mitochondrial gene rearrangements in the hermit crab Pagurus longicarpus (Crustacea, Anomura). Molecular Biology and Evolution, 17, 639–644.
Hilgendorf, F. (1879) Die von Hrn. W. Peters in Mocambique gesammelten Crustaceen. Monatsbericht der Koniglich Preussichen Akademie der Wissenschaften zu Berlin, 1878 (1879), 782–851.
Jobb, G. (2008) TREEFINDER ver. Jan 2008. Munich, Germany. www.treefinder.de
Krauss, F. (1843) Die Sudafrikanischen Crustaceen. Eine Zusammenstellung aller bekannten Malacostraca. Bemerkungen uber deren Lebensweise und geographische Verbreitung, nebst Beschreibung und Abbildung mehrerer neuen Arten. E. Schweizerbart’sche Verlagsbuchhandlung, Stuttgart, 68 pp.
Leach, W.E. (1820) Galatéadées. Dictionnaire des Sciences Naturelles, 18, 49–56. F. G. Levrault, Strasbourg and Le Normant, Paris.
Linnnaeus, C. (1758) Systema naturae per regna tria naturae, secundum classes, ordines, genera, species, cum characteribus, differentiis, synonymis, locis. Tomus I. Editio decima, reformata. Holmiae, ii + 824 pp.
Machordom, A. & Macpherson, E. (2004) Rapid radiation and cryptic speciation in squat lobsters of the genus Munida (Crustacea, Decapoda) and related genera in the South West Pacific: molecular and morphological evidence. Molecular Phylogenetics and Evolution, 33, 259–279.
Mantelatto, F.L.M., Robles, R., Biagi, R. & Felder, D.L. (2006) Molecular analysis of the taxonomic and distributional status for the hermit crab genera Loxopagurus Forest, 1964 and Isocheles Stimpson, 1858 (Decapoda, Anomura, Diogenidae). Zoosystema, 28, 495–506.
McLaughlin, P.A. (2002) A review of the hermit crab (Decapoda: Anomura: Paguridea) fauna of southern Thailand, with particular emphasis on the Andaman Sea, and descriptions of three new species. In: Bruce, N., Berggren, M. & Bussarawit, S. (Eds), Proceedings of the International Workshop on the Crustacea in the Andaman Sea, Phuket Marine Biological Center 29 November–20 December 1998. Phuket Marine Biological Center Special Publication, 23, 385–460.
McLaughlin, P.A., Rahayu, D.L., Komai, T. & Chan, T.Y. (2007) A Catalog of the Hermit Crabs (Paguroidea) of Taiwan. National Taiwan Ocean University, Keelung, viii + 365 pp.
Milne Edwards, H. (1848) Note sur quelques nouvelles especes du genre Pagure. Annales des Sciences Naturelles Zoologie, Paris, (3)10, 59–64.
Morrison, C.L., Harvey, A.W., Lavery, S., Tieu, K., Huang, Y. & Cunningham, C.W. (2002) Mitochondrial gene rearrangements confirm the parallel evolution of the crab-like form. Proceedings of the Royal Society of London. Series B, Biological Sciences, 269, 345–350.
Nobili, G. (1901) Viaggio del Dr. Enrico Festa nella Repubblica dell'Ecuador e regioni vicine. XXIII. Decapodi e Stomatopodi. Bollettino dei Musei di Zoologia ed Anatomia comparata della R. Università di Torino, 16(415), 1–58.
Osawa, M. & Chan, T.Y. (2009) Additional records of hermit crabs (Crustacea: Decapoda: Anomura: Paguroidea) from Taiwan. Proceedings of the Biological Society of Washington, 122(3), 317–332.
Osawa, M. & Fujita, Y. (2006) Clibanarius ambonensis (Crustacea: Decapoda: Anomura: Diogenidae) from the Ryukyu Islands, south-western Japan. JMBA2 Biodiversity Records, 5166, 1–8.
Pérez-Losada, M., Jara, C.C., Bond-Buckup, G. & Crandall, K.A. (2002) Phylogenetic relationships among the species of Aegla (Anomura: Aeglidae) freshwater crabs from Chile. Journal of Crustacean Biology, 22, 304–313.
Podsiadlowski, L. & Bartolomaeus, T. (2005) Organization of the mitochondrial genome of Mantis shrimp Peudosquilla ciliata (Crustacea: Stomatopoda). Marine Biotechnology, 7, 618–624.
Posada, D. & Crandall, K.A. (1998) Modeltest: Testing the model of DNA substitution. Bioinformatics, 14, 817–818.
Poupin J. & Malay M.C. (2009) Identification of a Ciliopagurus strigatus (Herbst, 1804) species-complex, with description of a new species from French Polynesia (Crustacea, Decapoda, Anomura, Diogenidae). Zoosystema, 31, 209–232.
Randall, J.W. (1840) Catalogue of the Crustacea brought by Thomas Nuttall and J.K. Townsend, from the west coast of North America and the Sandwich Islands, with descriptions of such species as are apparently new, among which are included several species of different localities, previously existing in the collection of the Academy. Journal of the Academy of Natural Sciences of Philadelphia, 8, 106–147.
Rahayu, D.L. & Forest, J. (1993) Le genre Clibanarius (Crustacea, Decapoda, Diogenidae) en Indonésie, avec la description de six espèces nouvelles. Bulletin du Muséum national d’Histoire naturelle, Paris, (4)14(A), 745–779.
Say, T. (1817–1818) An account of the Crustacea of the United States. Journal of the Academy of Natural Sciences of Philadelphia, 1, 57–63, 65–80, (pl. 4), 97–101, 155–160, 161–169 [all 1817], 235–253, 313–316, 317–319, 374–380, 381–401, 423–441 [all 1818].
Swofford, D. (2003) PAUP 4.0b7a,- Phylogenetic Analysis Using Parsimony (and Other Methods). Sinauer Associates, Sunderland, Massachuessetts.
Thompson, J.D., Higgins, D.G. & Gibson, T.J. (1994) ClustalW: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research, 22, 4673–4680.
Tirelli, T., Pessani, D., Silvestro, D. & Tudge, C. (2008) Reproductive biology of Mediterranian hermit crabs: fine structure of spermatophores and spermatozoa of Diogenes pugilator (Decapoda: Anomura) and its bearing on a sperm phylogeny of Diogenidae. Journal of Crustacean Biology, 28, 534–542.