Abstract
Two new Stephanostomum-like cercariae, Cercaria capricornia VII and Cercaria capricornia VIII (Digenea: Acanthocolpidae), are described from the nassariid gastropods Nassarius dorsatus and Nassarius olivaceus collected from the intertidal zone in the Capricornia region of Central Queensland, Australia. Morphological analysis of these new cercariae was augmented with DNA sequence data from the large subunit (LSU) ribosomal DNA region to aid in identification. Bayesian inference analysis of the LSU rDNA revealed that these putative acanthocolpid cercariae nested well within a clade containing species of Stephanostomum, which along with morphological data, suggests they are species of Stephanostomum. Comparative analysis of LSU rDNA sequences also indicates that these two cercariae are not S. adlardi, S. bicoronatum, S. tantabiddii or S. cf. uku, all species known from Australian fishes. The secondary structure of the internal transcribed spacer 2 (ITS2) rDNA region was inferred for these two cercariae using minimum free energy modelling algorithms. Both cercarial types displayed a four helix ITS2 secondary structure model and differed from each other by two compensatory base changes (CBCs) and nine hemi-CBCs.References
Barnett, L.J., Smales, L.R. & Cribb, T.H. (2008) A complex of putative acanthocolpid cercariae (Digenea) from Nassarius olivaceus and N. dorsatus (Gastropoda: Nassariidae) in Central Queensland, Australia. Zootaxa, 1705, 21–39.
Bartoli, P., Bray, R.A. & Montero, F.E. (2004) Tormopsolus orientalis Yamaguti, 1934 (Digenea: Acanthocolpidae) from Seriola dumerili (Risso) (Perciformes: Carangidae) in the western Mediterranean Sea. Systematic Parasitology, 57, 201–209.
Bartoli, P. & Gibson, D.I. (1998) A new acanthocolpid cercaria (Digenea) from Cantharus dorbignyi (Prosobranchia) in the Western Mediterranean. Systematic Parasitology, 40, 175–184.
Bouchet, P. & Rocroi, J.-P. (Eds) (2005) Classification and Nomenclator of Gastropod Families. Malacologia: International Journal of Malacology, 47, 1–397.
Bray, R.A. (2005). Family Acanthocolpidae Lühe, 1906. In: Jones, A., Bray, R.A. & Gibson, D.I. (Eds.), Keys to the Trematoda, Volume 2, CABI Publishing, pp. 603–619.
Bray, R., Cribb, T., Waeschenbach, A. & Littlewood, D. (2007) A new species of Stephanostomum Looss, 1899 (Digenea, Acanthocolpidae) with a bizarre oral sucker: S. adlardi sp. nov. from the common coral trout Plectropomus leopardus (Lacepède, 1802) (Perciformes, Serranidae) from Lizard Island, Great Barrier Reef. Acta Parasitologica, 52, 206–212.
Bray, R.A. & Cribb, T.H. (2000) Venusicola inusitatus gen. n., sp. n. (Digenea, Acanthocolpidae) from the Venus tuskfish Choerodon venustus (De Vis) from the southern Great Barrier Reef. Acta Parasitologica, 45, 295–298.
Bray, R.A. & Cribb, T.H. (2001) Tormopsolus attenuatus n. sp. (Digenea: Acanthocolpidae) from Seriola hippos (Perciformes: Carangidae), Western Australia, with some observations on the relationships in the genus. Systematic Parasitology, 50, 91–99.
Bray, R.A. & Cribb, T.H. (2002) Monostephanostomum georgianum n. sp. (Digenea: Acanthocolpidae) from Arripis georgianus (Valenciennes) (Perciformes: Arripidae) off Kangaroo Island, South Australia, with comments on Monostephanostomum Kruse, 1979 and Stephanostomum Looss, 1899. Systematic Parasitology, 53, 61–68.
Bray, R.A. & Cribb, T.H. (2003) Species of Stephanostomum Looss, 1899 (Digenea: Acanthocolpidae) from fishes of Australian and South Pacific waters, including five new species. Systematic Parasitology, 55, 159–197.
Bray, R.A. & Cribb, T.H. (2004) Stephanostomum tantabiddii n. sp. (Digenea: Acanthocolpidae) from Carangoides fulvoguttatus (Forsskål, 1775) (Perciformes: Carangidae) from Ningaloo Reef, Western Australia. Zootaxa, 457, 1–8.
Bray, R.A. & Cribb, T.H. (2006) Stephanostomum talakitok n. sp. (Digenea: Acanthocolpidae) from the golden trevalley, Gnathanodon speciosus (Perciformes: Carangidae), from Ningaloo Reef, Western Australia. Zootaxa, 1104, 59–68.
Bray, R.A. & Cribb, T.H. (2007a) Monostephanostomum nolani sp. n. and M. krusei Reimer, 1983 (Digenea: Acanthocolpidae) from carangid fishes from coral reef waters off Australia. Folia Parasitologica, 54, 19–26.
Bray, R.A. & Cribb, T.H. (2007b) Ningalooia psammopercae n. g., n. sp. (Digenea: Acanthocolpidae) from the Waigieu seaperch Psammoperca waigiensis (Cuvier) (Perciformes: Latidae) on the Ningaloo Reef, Western Australia. Systematic Parasitology, 66, 131–135.
Bray, R.A. & Cribb, T.H. (2008) Stephanostomum spp. (Digenea: Acanthocolpidae) from scombrids and carangids (Perciformes) from the Great Barrier Reef, with the description of two new species. Revista Mexicana de Biodiversidad, 79, 49S–68S.
Bray, R.A. & Gibson, D.I. (1991) The Acanthocolpidae (Digenea) of fishes from the north-east Atlantic: the status of Neophasis Stafford, 1904 (Digenea) and a study of North Atlantic forms. Systematic Parasitology, 19, 95–118.
Bray, R.A., Webster, B.L., Bartoli, P. & Littlewood, D.T.J. (2005) Relationships within the Acanthocolpidae Lühe, 1906 and their place among the Digenea. Acta Parasitologica, 50, 281–291.
Byun, Y. & Han, K. (2006) Pseudoviewer: web application and web service for visualizing RNA pseudoknots and secondary structures. Nucleic Acids Research, 34, W416–W422.
Cable, R.M. (1956) Marine cercariae of Puerto Rico. Scientific Survey of Porto Rico and the Virgin Islands, 16, 491–577.
Cable, R.M. (1963) Marine cercariae from Curaçao and Jamaica. Zeitschrift für Parasitenkunde, 23, 429–469.
Chubrik, G.K. (1966) Fauna and ecology of trematode larvae from molluscs in the Barents and White Seas. Trudy Murmanskii Biologicheskii Institut, 10, 78–166.
Coleman, A.W. (2003) ITS2 is a double-edged tool for eukaryote evolutionary comparisons. Trends in Genetics, 19, 370–375.
Coleman, A.W. (2007) Pan-eukaryote ITS2 homologies revealed by RNA secondary structures. Nucleic Acids Research, 35, 3322–3329.
Coleman, A.W. (2009) Is there a molecular key to the level of "biological species" in eukaryotes? A DNA guide. Molecular Phylogenetics and Evolution, 50, 197–203.
Cribb, T.H., Anderson, G.R., Adlard, R.D. & Bray, R.A. (1998) A DNA-based demonstration of a three-host life-cycle for the Bivesiculidae (Platyhelminthes: Digenea). International Journal for Parasitology, 28, 1791–1795.
Cribb, T.H., Bray, R.A. & Littlewood, D.T.J. (2001) The nature and evolution of the association between digeneans, molluscs and fishes. International Journal for Parasitology, 31, 997–1011.
Cribb, T.H., Chisholm, L.A. & Bray, R.A. (2002) Diversity in the Monogenea and Digenea: does lifestyle matter? International Journal for Parasitology, 32, 321–328.
Gnana Mani, G. (1994) Studies on Indian marine cercariae: a new acanthocolpid cercaria. Indian Journal of Parasitology, 18, 75–78.
Hassanine, R.M.E. (2006) The life cycle of Diploproctodaeum arothroni Bray and Nahhas, 1998 (Digenea: Lepocreadiidae), with a comment on the parasitic castration of its molluscan intermediate host. Journal of Natural History, 40, 1211–1222.
Holliman, R.B. (1961) Larval trematodes from the Apalachee Bay Area, Florida, with a checklist of known marine cercariae arranged in a key to their superfamilies. Tulane Studies in Zoology and Botany, 9, 1–74.
Hutson, K.S., Ernst, I., Mooney, A.J. & Whittington, I.D. (2007) Metazoan parasite assemblages of wild Seriola lalandi (Carangidae) from eastern and southern Australia. Parasitology International, 56, 95–105.
Køie, M. (1971) On the histochemistry and ultrastructure of the redia of Neophasis lageniformis (Lebour, 1910) (Trematoda, Acanthocolpidae). Ophelia, 9, 113–143.
Køie, M. (1973) The ultrastructure of the caecal epithelium of the intraredial cercaria of Neophasis lageniformis (Lebour, 1910) (Trematoda, Acanthocolpidae). Cell and Tissue Research, 139, 405–416.
Køie, M. (1978) On the morphology and life-history of Stephanostomum caducum (Looss 1901) Manter 1934 (Trematoda, Acanthocolpidae). Ophelia, 17, 121–133.
Larkin, M.A., Blackshields, G., Brown, N.P., Chenna, R., McGettigan, P.A., McWilliam, H., Valentin, F., Wallace, I.M., Wilm, A., Lopez, R., Thompson, J.D., Gibson, T.J. & Higgins, D.G. (2007) Clustal W and Clustal X version 2.0. Bioinformatics, 23, 2947–2948.
Littlewood, D.T., Curini-Galletti, M. & Herniou, E.A. (2000) The interrelationships of Proseriata (Platyhelminthes: Seriata) tested with molecules and morphology. Molecular Phylogenetics and Evolution, 16, 449–466.
MacKenzie, K. & Liversidge, J.M. (1975) Some aspects of the biology of the cercaria and metacercaria of Stephanostomum baccatum (Nicoll, 1907) Manter, 1934 (Digenea: Acanthocolpidae). Journal of Fish Biology, 7, 247–256.
Maddison, D.R. & Maddison, W.P. (2005) MacClade 4: Analysis of phylogeny and character evolution. Version 4.08. Sinauer Associates, Sunderland, Massachusetts.
Madhavi, R. & Shameem, U. (1993) Cercariae and metacercariae of Stephanostomum cloacum (Trematoda: Acanthocolpidae). International Journal for Parasitology, 23, 341–347.
Maillard, C. (1975) Cycle evolutif de Paratimonia gobii Prevot et Bartoli, 1967 (Trematoda: Monorchiidae). Acta tropica, 32, 327–333.
Marasaev, S.F. (1984) Three types of cercariae from benthic molluscs of the central and south-east parts of the Barents Sea. In: Polyansky, Y.I., Galaktionov, K.V. & Dobrovolsky, A.A. (Eds.) Ecological-parasitological investigations of northern seas. Apatity: Kola Branch of Academy of Sciences of the USSR, Murmansk Biological Institute, Murmansk, pp. 51–59.
Martin, W.E. (1939) Studies on the trematodes of Woods Hole: II. The life cycle of Stephanostomum tenue (Linton). Biological Bulletin, 77, 65–73.
Miller, H.M., Jr. & Northup, F.E. (1926) The seasonal infestation of Nassa obsoleta (Say) with larval trematodes. Biological Bulletin, 50, 490–508.
Miller, T.L. & Cribb, T.H. (2009) Gynichthys diakidnus n. g., n. sp. (Digenea: Cryptogonimidae) from the grunt Plectorhinchus gibbosus (Lacépède, 1802) (Perciformes: Haemulidae) off the Great Barrier Reef, Australia. Systematic Parasitology, 74, 103–112.
Morgan, J.A.T. & Blair, D. (1995) Nuclear rDNA ITS sequence variation in the trematode genus Echinostoma: an aid to establishing relationships within the 37-collar-spine group. Parasitology, 111, 609–615.
Morgan, J.A.T. & Blair, D. (1998) Trematode and monogenean rRNA ITS2 secondary structures support a four-domain model. Journal of Molecular Evolution, 47, 406–419.
Müller, T., Philippi, N., Dandekar, T., Schultz, J. & Wolf, M. (2007) Distinguishing species. RNA, 13, 1469–1472.
Nasir, P. (1976) Marine larval trematodes. I. Cercariae criollisimae: five new Venezuelan species and redescription of Parorchis acanthus (Nicoll, 1906) Nicoll, 1907. Rivista di Parassitologia, 37, 287–302.
Posada, D. & Crandall, K.A. (1998) Modeltest: testing the model of DNA substitution. Bioinformatics, 14, 817–818.
Ronquist, F. & Huelsenbeck, J.P. (2003) MRBAYES 3: Bayesian phylogenetic inference under mixed models. Bioinformatics, 19, 1572–1574.
Schultz, J., Maisel, S., Gerlach, D., Müller, T. & Wolf, M. (2005) A common core of secondary structure of the internal transcribed spacer 2 (ITS2) throughout the Eukaryota. RNA, 11, 361–364.
Seibel, P.N., Müller, T., Dandekar, T. & Wolf, M. (2008) Synchronous visual analysis and editing of RNA sequence and secondary structure alignments using 4SALE. BMC Research Notes, 1, 91.
Shimura, S. (1984) A new species of marine cercaria Cercaria itoi sp. nov. from the spindle shell Fusinus perplexus from Kanagawa Prefecture, Japan. Fish Pathology, 18, 179–183.
Stunkard, H.W. (1961) Cercaria dipterocerca Miller and Northup, 1926 and Stephanostomum dentatum (Linton, 1900) Manter, 1931. Biological Bulletin, 120, 221–237.
Wardle, W.J. (1975) Cercaria anadarae sp. n. parasitizing a bivalve mollusc, Anadara brasiliana (Lamarck), from the northwest Gulf of Mexico. Journal of Parasitology, 61, 1048–1049.
Wolf, M., Friedrich, J., Dandekar, T. & Müller, T. (2005) CBCAnalyzer: inferring phylogenies based on compensatory base changes in RNA secondary structures. In Silico Biology, 5, 0027.
Wolfgang, R.W. (1955) Studies of the trematode Stephanostomum baccatum (Nicoll, 1907). III. Its life cycle. Canadian Journal of Zoology, 33, 113–128.
Yamaguti, S. (1971) Synopsis of digenetic trematodes of vertebrates. Keigaku Publishing Co., Tokyo, 1074 pp.
Yamaguti, S. (1975) A synoptical review of life histories of digenetic trematodes of vertebrates: with special reference to the morphology of their larval forms. Keigaku Publishing Co., Tokyo, 590 pp.
Zuker, M. (2003) Mfold web server for nucleic acid folding and hybridization prediction. Nucleic Acids Research, 31, 3406–3415.