Abstract
A new species of Acanthosaura (Gray 1831), A. cardamomensis sp. nov. from eastern Thailand and western Cambodia is described based on having a combination of unique morphological characteristics. It most closely resembles A. crucigera but differs in having significantly longer postorbital spines, occipital spines, nuchal spines, and dorsal spines; more scales between the fifth canthals; longer maximum dorsal scale length; longer maximum nuchal scale length; a significantly smaller diastema between the nuchal and dorsal crest spines; and significantly more supralabials. Mitochondrial DNA was used to calculate percent sequence divergence between samples of A. crucigera from southern Myanmar and western Thailand and samples of A. cardamomensis sp. nov. from eastern Thailand and western Cambodia. The uncorrected pairwise sequence divergence between populations of A. cardamomensis ranged from 1.2– 3.1% and the range between populations of A. crucigera was 3.8%. The percent sequence divergence between A. cardamomensis sp. nov. and A. crucigera ranged between 10.0–14.6%.References
Ananjeva, N.B., Orlov, N.L. & Kalyabina-Hauf, S.A. (2008) Species of Acanthosaura Gray, 1831 (Agamidae: Sauria, Reptilia) of Vietnam: results of Molecular and Morphological study. Biology Bulletin, 35(2), 178–186.
Boulenger, G.A. (1885) Catalogue of the Lizards in the British Museum (Natural History). Second edition. Vol. I. Geckonidae, Eublepharidae, Uroplatidae, Pygopodidae, Agamidae. British Museum (Natural History), London. xii + 436 pp + Pl. I–XXXII.
Boulenger, G.A. (1912) A Vertebrate Fauna of the Malay Peninsula from the Isthmus of Kra to Singapore including the Adjacent Islands. Reptilia and Batrachia. Taylor and Francis, London, xiv, 294 pp.
Daltry, J.C. & Chheang, D. (2000) Reptiles. In Cardamom Mountains Biodiversity Survey 2000 (eds Daltry, J.C. & Momberg, F.), Fauna & Flora International, Cambridge, UK.
Daltry, J.C. & Traeholt, C. (2003) Reptile and amphibian survey. In Biodiversity Assessment of the Southern Cardamoms and Botum-Sakor Peninsula (eds Daltry, J.C. & Momberg, F.), pp. 82–100 and Appendices III and IV. WildAid: Cambodia Program, and Department of Forestry and Wildlife, Phnom Penh, Cambodia.
Drummond, A.J., Kearse, M., Heled, J., Moir, R., Thierer, T. & Ashton, B. (2006) Geneious v1.2.1, Available from http://www.geneious.com/
Gray, J.E. (1831) A synopsis of the species of the class reptilia. Bredell, South Africa: Herprint 1996.
Gray, J.E. (1845) British Museum (Natural History), Catalogue of the Specimens of Lizards in the Collection of the British Museum. London: Trustees of the British Museum, London. Pp. 240.
Grismer, J.L., Grismer, L.L. & Chav, T.A. (in press) new species of Cnemaspis Strauch, 1887 (Squamata: Gekkonidae) from Southwestern Cambodia. Journal of Herpetology.
Grismer, L.L., Youmans, T.M., Wood, Jr., P.L., Ponce, A., Johnson, R., Wright, S.B. & Norsham, S.Y. (2006) Checklist of the herpetofauna of Pulau Langkawi with taxonomic comments. Hamadryad, 29, 15–32.
Grismer, L.L., Chav T., Neang T., Wood Jr, P.L., Grismer, J.L., Youmans, T.M., Ponce, A., Daltry, J.C. & Kaiser, H. (2007) The herpetofauna of the Phnom Aural Wildlife Sanctuary and checklist of the herpetofauna of the Cardamom Mountains, Cambodia. Hamadryad, 31, 216–241.
Grismer, L.L., Neang, T., Chav, T., Wood, Jr, P. L., Oaks, J.R., Holden, J., Grismer, J.L., Szutz, T.R. & Youmans, T.M. (2008a) Additional amphibians and reptiles from the Phnom Samkos Wildlife Sanctuary in the northwestern Cardamom Mountains, Cambodia, with comments on their taxonomy and the discovery of three new species. The Raffles Bulletin of Zoology, 56, 161–175.
Grismer, L.L., Neang, T., Chav, T. & Grismer, J.L. (2008b) Checklist of the amphibians and reptiles of the Cardamom region of southwestern Cambodia. Cambodian Journal of Natural History 2008 (1) 12–28.
Günther, A.C.L.G. (1861) Second list of Siamese reptiles. Proceedings of the Zoological Society of London, 1861(8), 135–140.
Günther, A.C.L.G. (1864) The Reptiles of British India. Ray Society, London, xxviii, 452, 26 lithographed plates, 19 text figs.
Hardwicke, T. & Gray, E.J. (1828) Synopsis of the species of saurian reptiles collected in India. Zoological Journal, 3: 213.
Jackman, T.R., Bauer, M.A., Greenbaum, E., Glaw, F. & Vences, M. (2008) Molecular phylogenetic relationships among species of the Malagasy-Comoran gecko genus Paroedura (Squamata: Gekkonidae). Molecular Phylogenetics and Evolution 46, 74–81.
Kalyabina-Hauf, S., Ananjeva, N.B., Joger, U., Lenk, P., Murphy, R.W., Stuart, B.L., Orlov, N.L., Ho, C.T. & Wink, M. (2004) Molecular phylogeny of the genus Acanthosaura (Agamidae). Current Herpetology. 23, (1), 7–16.
Leong, T.M., Grismer, L.L. & Mumpuni (2002) Preliminary checklists of the herpetofauna of the Anambas and Natuna Islands (South China Sea). Hamadryad, 27(2), 165–174.
Macey, J.R., Larson, A., Ananjeva, N.B., Fang, Z. & Papenfuss, T.J. (1997) Two novel gene orders and the role of light-strand replication in rearrangement of the vertebrate mitochondrial genome. Molecular Biology and Evolution 14, 91–104.
Maddison, W.P. & Maddison, D.R. (1992) MacClade version 3: Analysis of Phylogeny and Character Evolution. Sinauer Associates, Sunderland, Massachusetts.
Orlov, N.L., Truong, N.Q. & Nguyen, V.S. (2006) A New Acanthosaura allied to A. capra Günther, 1861 (Agamidae, Sauria) from Central Vietnam and Southern Laos. Russian Journal of Herpetology 13(1), 61–76.
Pauwels, O.S.G., David, P., Chucheep, C. & Kumthorn, T. (2003) Reptiles of Phetchaburi Province, western Thailand: a list of species, with natural history notes, and a discussion on the biogeography at the Isthmus of Kra. The Natural History Journal of Chulalongkorn University, 3(1), 23–53.
Smith, M.A. (1935) The fauna of British India, Including Ceylon and Burma. Reptilia and Amphibia, Vol II. Sauria. Taylor and Francis, London xiii + 440 pp., 1 pl.
Stuart, B.L. & Emmett, D.A. (2006) A collection of amphibians and reptiles from the Cardamom Mountains, Southwestern Cambodia. Fieldiana, No. 109, 1–17.
Stuart, B.L., Sok, K. & Neang, T. (2006) A collection of amphibians and reptiles from hilly eastern Cambodia. The Raffles Bulletin of Zoology, 54, 129–155.
Swofford, D.L. (2002) PAUP* Phylogenetic Analysis Using Parsimony (* and other Methods). Sinauer Associates, Sunderland, Massachusetts.
Taylor, E.H. (1963) The lizards of Thailand. Kansas University Science Bulletin, 46(5), 687–1077.
Manthey, U. (2008) Agamid Lizards of Southern Asia, Draconinae 1, (Terralog 7a). Edition Chimaira/ Verlag ACS GmbH, Frankfurt/M./Rodgau.
Manthey, U. & Grossmann, W. (1997) Amphibien & Reptilien Südostasiens. Natur und Tier-Verlag, Münster, 512 pp.
Wood, P.L. Jr., Grismer, J.L., Grismer, L.L., Norhayati, A., Onn, C.K. & Bauer, A.M. (2009) Two new montane species of Acanthosaura Gray, 1831 (Squamata: Agamidae) from Peninsular Malaysia. Zootaxa, 2012, 28–46.