Abstract
Allozyme electrophoresis is used to explore molecular genetic relationships within the Rana arfaki group and between this group and selected lineages of New Guinean Rana. Rana jimiensis is confirmed as a species distinct from R. arfaki and its range in Papua New Guinea is extended to the Southern Highlands Province and the north-coastal ranges in Sandaun Province. Rana arfaki and R. jimiensis show a high level of genetic differentiation maintained across a wide geographic area and show consistent morphological differences in head shape, tympanum size, degree of digital disc dilation and extent of sexual dimorphism. The two species occur syntopically on the Papuan Plateau, Southern Highlands Province, and are regionally sympatric in Sandaun Province. The observed level of genetic differentiation is equivalent to that reported previously between regionally sympatric members of the Rana papua group. Populations of R. jimiensis from north and south of the central cordillera show no obvious morphological and only minor genetic differentiation. In contrast, R. arfaki shows considerable geographic variation in both morphology and allozymes and may include two or more regionally distinctive forms.References
Cavalli-Sforza, L.L. & Edwards, A.W.F. (1967) Phylogenetic analysis: models and estimation procedures. Evolution, 3, 550–570.
Che, J., Pang, J., Zhao, H., Wu, G., Zhao, E. –M. & Zhang, Y. –P. (2007) Phylogeny of Raninae (Anura: Ranidae) inferred from mitochondrial and nuclear sequences. Molecular Phylogenetics and Evolution, 43, 1–13.
Colgan, D., Flannery, T. F., Trimble, J. & Aplin, K.P. (1993) Electrophoretic and morphological analysis of the systematics of the Phalanger orientalis (Marsupialia) species complex in New Guinea and the Solomon Islands. Australian Journal of Zoology, 41, 355–378.
Donnellan, S. C., Adams, M. & Aplin, K.P. (1989) A biochemical and morphological study of Rana (Anura: Ranidae) from the Chimbu Province, Papua New Guinea. Herpetologica, 45, 336–343.
Donnellan, S.C. & Aplin, K.P. (1989) Resolution of cryptic species in the New Guinean lizard, Sphenomorphus jobiensis (Scincidae) by electrophoresis. Copeia, 1989, 81–88.
Dubois, A. (1992) Notes sur la classification des Ranidae (Amphibiens Anoures). Bulletin Mensuel de la Societe Linneene de Lyon, 6, 305–352.
Felsenstein, J. (1993) PHYLIP—Phylogeny Inference Package Version 3.5c. Available from http://evolution.genetics.washington.edu/phylip.html. (accessed 14 October 2003)
Flannery, T. F., Colgan, D. & Trimble, J. (1994) A new species of Melomys from Manus Island, Papua New Guinea, with notes on the systematics of the M. rufescens complex (Muridae: Rodentia). Proceedings of the Linnean Society of New South Wales, 114, 29–43.
Frost, D. R. (2010) Amphibian Species of the World 5.3, an online reference. (accessed 30 January 2010).
Frost, D. R., Grant, T., Faivovich, J,, Bain, R., Haas, A., Haddad, C. F. B., de SA, R. O., Channing, A., Wilkinson, M., Donnellan, S. C., Raxworthy, C., Campbell, J. A., Blotto, B. L., Moler, P., Drewes, R., Nussbaum, R. A., Lynch, J. D., Green, D. & Wheeler, W. (2006) The amphibian tree of life. Bulletin of the American Museum of Natural History, 297, 1–370.
Günther, R. (2003) Sexual color dimorphism in ranid frogs from New Guinea: description of two new species (Amphibia, Anura, Ranidae). Mitteilungen aus dem Museum für Naturkunde in Berlin, Zoologische Reihe, 79, 207–227.
Hillis, D. M. & Wilcox, T. P. (2005) Phylogeny of the New World true frogs (Rana). Molecular Phylogenetics and Evolution, 34, 299–314.
Inger, R.F. (1996) Commentary on a proposed classification of the family Ranidae. Herpetologica, 52, 241–246.
Kraus, F. & Allison, A. (2007) Taxonomic notes on frogs of the genus Rana from Milne Bay Province, Papua New Guinea. Herpetological Monographs, 21, 33–75.
Menzies, J. I. (1987) A taxonomic revision of Papuan Rana (Amphibia: Ranidae). Australian Journal of Zoology, 35, 373–418.
Murphy, R. W., Sites, J. W., Buth, D. G. & Haufler, C. H. (1996) Proteins I: Isozyme Electrophoresis. In: Hillis D., Moritz, C. & Mable, B. (Eds.), Molecular Systematics. Sinauer, Sunderland, Massachusetts, pp. 51–120.
Rawlings, L. H. & Donnellan, S. C. (2003) Phylogeographic analysis of the green python, Morelia viridis, reveals cryptic diversity. Molecular Phylogenetics and Evolution, 27, 36–44.
Richards, S., Iskandar, D. & Allison, A. (2000) Species accounts of frogs collected on the RAP survey in the Wapoga area, Irian Jaya, Indonesia. Appendix 14, In: Mack, A.L. & Alonso, L.E. (Eds.), A Biological Assessment of the Wapoga River Area of Northwestern Irian Jaya, Indonesia. RAP Bulletin of Biological Assessment 14. Conservation International, Washington, DC, pp. 116–120.
Richardson, B.J., Baverstock, P.R. & Adams, M.A. (1986) Allozyme electrophoresis. Academic Press, Sydney, Australia, 410 pp.
Scott, E. (2005) A phylogeny of ranid frogs (Anura: Ranoidea: Ranidae), based on a simultaneous analysis of morphological and molecular data. Cladistics, 21, 507–574.
Statsoft Inc. (1997) STATISTICA for Windows [computer program manual]. Tulsa Okalahoma. StatSoft Inc. 2300 East 14th Street Tulsa OK 74104.
Swofford, D. (1999) PAUP*. Phylogenetic Analysis Using Parsimony. (* and Other Methods). Sinauer Associates, Sunderland, Masschusetts.
Swofford, D. & Selander, R. K. (1981) BIOSYS-1: a FORTRAN program for the comprehensive analysis of electrophoretic data in population genetics and systematics. The Journal of Heredity, 72, 281–283.
Tyler, M. J. (1963) An account of collections of frogs from central New Guinea. Records of the Australian Museum, 26, 113–130.