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Issue: Vol. 2509 No. 1: 17 Jun. 2010
Type: Articles
Published: 2010-06-17
DOI: 10.11646/zootaxa.2509.1.3
Page range: 39–52
Abstract views: 45
PDF downloaded: 3

Morphological and molecular characterization of Aphelenchoides fujianensis n. sp. (Nematoda: Aphelenchoididae) from Pinus massoniana in China

Laboratory of Plant Nematology, South China Agricultural University, Guangzhou 510642, China
Laboratory of Plant Nematology, South China Agricultural University, Guangzhou 510642, China Guangdong Entry-Exit Inspection and Quarantine Bureau, Guangzhou, China
Nematode Assay Section, Agronomic Division, North Carolina Department of Agriculture & Consumer Services, 4300 Reedy Creek Road, NC 27607, USA
Laboratory of Plant Nematology, South China Agricultural University, Guangzhou 510642, China Zhongkai University of Agriculture and Engineering, Guangzhou, China
Laboratory of Plant Nematology, South China Agricultural University, Guangzhou 510642, China
Guangdong Entry-Exit Inspection and Quarantine Bureau, Guangzhou, China
Laboratory of Plant Nematology, South China Agricultural University, Guangzhou 510642, China
Nematoda Aphelenchoididae Aphelenchoides fujianensis n. sp. China description DNA sequencing molecular phylogeny morphology SEM taxonomy

Abstract

Aphelenchoides fujianensis n. sp. is described and illustrated from a dead Pinus massoniana based on morphology and molecular analyses of the near-full-length small subunit rDNA gene and partial cytochrome oxidase subunit I of mitochondrial DNA. This new species belongs to the Group 3 of Aphelenchoides species sensu Shahina with star-shaped tail terminus and is characterised by a relatively long body (653–843 μ m in the male and 803–941 μ m in the female) and four lateral incisures in the lateral field. The male has relatively large spicules (24–30 μ m). The female has elongate postvulval uterine sac (extending ca 32–44% of vulva-anus distance), usually with sperms. Both male and female have star-shaped mucro. It is distinguished from other species by postvulval uterine sac length, a and c ratios, and spicule size and shape. Molecular analysis reveals that this species has unique 18S and mt-DNA sequences, and is closest to Aphelenchoides besseyi in dendrograms inferred using both markers. The identification codes of OEPP/EPPO for A. fujianensis n. sp. are: A1-B2-C1-D1/3-E1-F1/2.

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