Abstract
A new species of Crenicichla Heckel, C. hu, is described from the arroyo Piray–Miní, a left-hand tributary of the río Paraná, Misiones province, Argentina. This new species is easily distinguished from its congeners in the La Plata basin and adjacent coastal rivers by the dark coloration (dark grey or dark brown to black), a color pattern consisting of 7 to 9 black irregular blotches on the flank, and 47–54 scales in the E1 row. Adult females have dorsal fin with an irregular color pattern formed by wide black and white longitudinal stripes and blotches. In addition to standard morphological comparisons, a brief molecular phylogenetic analysis of Crenicichla species from the province of Misiones is also introduced.References
Bertonatti, C. & Corcuera, J. (2000) Situación Ambiental Argentina. Fundación Vida Silvestre, Buenos Aires, 400 pp.
Casciotta, J.R. (1987) Crenicichla celidochilus n. sp. from Uruguay and a multivariate analysis of the lacustris group (Perciformes, Cichlidae). Copeia, 1987, 883–891.
Casciotta, J. & Almirón, A. (2008) Crenicichla tesay, a new species of cichlid (Perciformes: Labroidei) from the río Iguazú basin in Argentina. Revue suisse de Zoologie, 115, 651–659.
Casciotta, J.R., Almirón, A.E. & Gómez, S.E. (2006) Crenicichla yaha sp. n. (Perciformes: Labroidei: Cichlidae), a new species from the río Iguazú and arroyo Urugua–í basins, northeastern Argentina. Zoologische Abhandlungen, Staatliche Naturhistorische Sammlungen Dresden, Museum für Tierkunde, 56, 107–112.
Casciotta, J.R. & Arratia, G. (1993) Jaws and teeth of American Cichlids (Pisces: Labroidei). Journal of Morphology, 217, 1–36.
Farias, I.P., Ortí, G. & Meyer, A. (2000) Total evidence: molecules, morphology, and the phylogenetics of cichlid fishes. Journal of Experimental Zoology, 288, 76–92.
Farias, I.P., Ortí, G., Sampaio, I., Schneider, H. & Meyer, A. (1999) Mitochondrial DNA phylogeny of the family Cichlidae: monophyly and fast molecular evolution of the Neotropical assemblage. Journal of Molecular Evolution, 48, 703–711.
Felsenstein, J. (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution, 39, 783–791.
Hall, T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41, 95–98.
Hall, T.A. (2001) Biolign alignment and multiple contig editor. Available from: http://en.bio-soft.net/dna/BioLign.html (accessed 19 June 2009).
Huelsenbeck, J.P. & Ronquist, F. (2001) MrBayes: Bayesian inference of phylogenetics trees. Bioinformatics, 17, 754–755.
Kullander, S.O. (1981) Cichlid fishes from the La Plata basin. part I. Collections from Paraguay in the Muséum d'Histoire naturelle de Genève. Revue suisse de Zoologie, 88, 675–692.
Kullander, S.O. (1982) Cichlid fishes from the La Plata basin. Part 3. The Crenicichla lepidota species group (Teleostei: Cichlidae). Revue suisse de Zoologie, 89, 627–661.
Kullander, S.O. (1986) Cichlid fishes of the Amazon River drainage of Peru. Swedish Museum of Natural History, Stockholm, 431 pp.
Kullander, S.O. (2009) Crenicichla mandelburgeri, a new species of cichlid fish (Teleostei: Cichlidae) from the Paraná River drainage in Paraguay. Zootaxa, 2006, 41–50.
Kullander, S.O. & Lucena, C.A.S. (2006) A review of the species of Crenicichla (Teleostei: Cichlidae) from the Atlantic coastal rivers of southeastern Brasil from Bahia to Rio Grande do Sul State, with description of three new species. Neotropical Ichthyology, 4, 127–146.
Kullander, S.O., Norén, M., Friðriksson, G.B. & Lucena, C.A.S. (in press) Phylogenetic relationships of species of Crenicichla (Teleostei: Cichlidae) from southern South America based on the mitochondrial cytochrome b gene. Journal of Zoological Systematics and Evolutionary Research, doi 10.1111/j.1439-0469.2009.00557.x.
Kutty, V. (2000) An introduction to the pike cichlids of the genus Crenicichla. Cichlid News, 9, 6–14.
Leviton, A.E., Gibbs Jr., R.H., Heal, E. & Dawson, C.E. (1985) Standards in herpetology and ichthyology: Part I. Standard symbolic codes for institutional resource collections in herpetology and ichthyology. Copeia, 1985, 802–832.
López, H.L., Morgan, C.C. & Montenegro, M.J. (2002) Ichthyological ecoregions of Argentina. Probiota: Serie Documentos N° 1, La Plata, Buenos Aires, Argentina, 53 pp.
López-Fernández, H., Honeycutt, R.L. & Winemiller, K.O. (2005) Molecular phylogeny and evidence for an adaptive radiation of geophagine cichlids from South America (Perciformes: Labroidei). Molecular Phylogenetics and Evolution, 34, 227–244.
Lucena, C.A.S. (2007) Two new species of the genus Crenicichla Heckel, 1840 from the upper rio Uruguay drainage (Perciformes: Cichlidae). Neotropical Ichthyology, 5, 449–456.
Lucena, C.A.S. & Kullander, S.O. (1992) The Crenicichla (Teleostei: Cichlidae) species of the Uruguai River drainage in Brazil. Ichthyological Exploration of Freshwaters, 3, 97–160.
Miquelarena, A.M., Protogino, L.C., Filiberto, R. & López, H.L. (2002) A new species of Bryconamericus (Characiformes: Characidae) from the Cuña–Pirú creek in north–eastern Argentina, with comments on accompanying fishes. Aqua, Journal of Ichthyology and Aquatic Biology, 6, 69–82.
Montaña, C.G., López-Fernández, H. & Taphorn, D.C. (2008) A new species of Crenicichla (Perciformes: Cichlidae) from the Ventuari River, Upper Orinoco River basin, Amazonas State, Venezuela. Zootaxa, 1856, 33–40.
Nylander, J.A.A. (2004) MrModeltest, Evolutionary Biology Centre, Uppsala Univ, Sweden. Available from: http://www.abc.se/~nylander (accessed 19 June 2009).
Pereyra, S. & García, G. (2008) Patterns of genetic differentiation in the Gymnogeophagus gymnogenys species complex, a neotropical cichlid from South American basins. Environmental Biology of Fishes, 83, 245–257.
Ploeg, A. (1991) Revision of the South American cichlid genus Crenicichla Heckel, 1840, with description of fifteen new species and consideration on species groups, phylogeny and biogeography (Pisces, Perciformes, Cichlidae). Univ. Amsterdam, Netherlands, 153 pp.
Posada, D. & Crandall, K.A. (1998) Modeltest: testing the model of DNA substitution. Bioinformatics, 14, 817–818.
Reis, R.E., Kullander, S.O. & Ferraris Jr., C.J. (2003) Check list of the freshwater fishes of South and Central America. Edipucrs, Porto Alegre, Brazil, 729 pp.
Resende, E.K. (2003) Migratory Fishes of the Paraguay–Paraná Basin, Excluding the Upper Paraná Basin. In: Carolsfeld, J., Harvey, B., Ross, C. & Baer, A. (Eds.), Migratory fishes of South America: Biology, Fisheries and Conservation Status. International Development Research Center and The World Bank, pp. 99–156.
Rodríguez, M.S. & Miquelarena, A.M. (2005) A new species of Rineloricaria (Siluriformes: Loricariidae) from the Paraná and Uruguay River basins, Misiones, Argentina. Zootaxa, 945, 1–15.
Ronquist, F. & Huelsenbeck, J.P. (2003) Mrbayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics, 19, 1572–1574.
Stawikowski, R. & Werner, U. (2004) Die Buntbarsche Amerikas. Band 3: Erdfresser, Hecht- und Kammbuntbarsche. Eugen Ulmer, Stuttgart, 478 pp.
Swofford, D.L. (2001) Paup*. Phylogenetic Analysis Using Parsimony (*and Other Methods), Sinauer Associates, Inc. Publishers, Sunderland, Massachusetts. Available from: http://paup.csit.fsu.edu (accessed 19 June 2009).
Taylor, W.R. & Van Dyke, G.C. (1985) Revised procedures for staining and clearing small fi shes and other vertebrates for bone and cartilage study. Cybium, 9, 107–119.
Wimberger, P.H., Reis, R.E. & Thornton, K.R. (1998) Mitochondrial Phylogenetics, Biogeography, and Evolution of Parental Care and Mating Systems in Gymnogeophagus (Perciformes: Cichlidae). In: Malabarba L.R., Reis, R.E., Vari, R.P., Z. M. S. Lucena & C. A. S. Lucena (Eds.), Phylogeny and Classification of Neotropical fishes. Porto Alegre, Edipucrs, pp. 69–84.
Zaniboni Filho, E. & Schulz, U.H. (2003) Migratory fishes of the Uruguay river. In: Carolsfeld, J., Harvey, B., Ross, C. & Baer, A. (Eds.), Migratory fishes of South America: Biology, Fisheries and Conservation Status. International Development Research Center and The World Bank, pp. 157–194.