Skip to main content Skip to main navigation menu Skip to site footer
Type: Articles
Published: 2010-09-14
Page range: 1–15
Abstract views: 42
PDF downloaded: 1

A diploid surrounded by polyploids: tadpole description, natural history and cytogenetics of Odontophrynus maisuma Rosset from Uruguay (Anura: Cycloramphidae)

Museo Nacional de Historia Natural, 25 de Mayo 582, Montevideo, Uruguay
Museo Nacional de Historia Natural, 25 de Mayo 582, Montevideo, Uruguay Universidad Católica del Uruguay, Montevideo, Uruguay
Laboratorio de Genética Evolutiva y Molecular, Facultad de Ciencias Exactas Químicas y Naturales, Universidad Nacional de Misiones. Félix de Azara Nº 1552. CP: 3300, Posadas, Misiones, Argentina División de Herpetología, Museo Argentino de Ciencias Naturales ‘‘Bernardino Rivadavia’’-CONICET, Ángel Gallardo 470 (1405), Buenos Aires, Argentina
Sección Herpetología, Museo de La Plata, Universidad Nacional de La Plata, Paseo del Bosque s/nº (1900), La Plata, Buenos Aires, Argentina
Laboratorio de Genética Evolutiva y Molecular, Facultad de Ciencias Exactas Químicas y Naturales, Universidad Nacional de Misiones. Félix de Azara Nº 1552. CP: 3300, Posadas, Misiones, Argentina Instituto de Herpetología, Fundación Miguel Lillo-CONICET, Miguel Lillo 251, Tucumán, Argentina
Amphibia Odontophrynus tadpole advertisement call Ag-NORs C-banding erythrocyte measurements

Abstract

Odontophrynus maisuma is a recently described diploid species of the diploid-tetraploid O. americanus complex. It inhabits coastal regions of Uruguay and southern Brazil. Tadpoles of O. maisuma at stages 33–36 are about 47 mm of total length, and similarly to that of other Odontophrynus are typical pond dwelling anuran larvae; the oral disc is small and laterally emarginated, bearing a single row of marginal papillae interrupted by a large dorsal gap. The labial tooth row formula is 2(2)/3(1); sometimes A2 and P1 are not divided. The advertisement call consists of notes that last 570– 785 ms, composed of 43–57 pulses, and with a dominant frequency between 1124–1211 Hz. The chromosome complement is composed by 11 biarmed chromosome pairs (2n = 2x = 22; NF = 44), with evident positive C-bands in the centromeric regions of all chromosome pairs, in the short arms of pairs 2 and 4, and in the long arms of pair 6 and 9. Nucleolar Organizer Regions (Ag-NORs) are located interstitially in the short arms of both chromosomes of pair 4. Preliminary data suggests that erythrocyte morphology could be used to discriminate between diploid and tetraploid tadpoles of Odontophrynus.

References

  1. Altig, R. & Johnston, G.F. (1989) Guilds of anuran larvae: Relationships among developmental modes, morphologies, and habitats. Herpetological Monographs, 3, 81–109.

    Altig, R. & McDiarmid, R.W. (1999) Body Plan. Development and Morphology. In: McDiarmid, R.W. & Altig, R. (Eds.), Tadpoles: The Biology of Anuran Larvae. The University of Chicago Press, Chicago, Illinois, USA, pp. 24–51.

    Barrio, A. (1964) Caracteres eto-ecológicos diferenciales entre Odontophrynus americanus (Duméril et Bibron) y O. occidentalis (Berg) (Anura, Leptodactylidae). Physis, 24, 385–390.

    Barrio, A. & Pistol de Rubel, D. (1972) Encuesta cariotípica de poblaciones argentino-uruguayas de Odontophrynus americanus (Anura, Leptodactylidae) relacionada con otros rasgos taxonómicos. Physis, 31, 281–291.

    Beçak, W., Beçak, M.L., Lavalle, D. & Schreiber, G. (1967) Further studies on polyploid amphibians (Ceratophrydae). II. DNA content and nuclear volume. Chromosoma, 23, 14–23.

    Beçak, M.L., Beçak, W. & Rabello, M.N. (1966) Cytological evidence of constant tetraploidy in the bisexual south american frog Odontophrynus americanus. Chromosoma, 19, 188–193.

    Beçak, M.L., Denaro, I. & Beçak, W. (1970) Polyploidy and mechanisms of karyotypic diversification in Amphibia. Cytogenetics, 9, 225–238.

    Blair, W.F. (1958) Mating call in the speciation of anuran amphibians. The American Naturalist, 92, 27–51.

    Bogart, J.P. (1967) Chromosomes of the south american amphibian Family Ceratophridae with a reconsideration of the taxonomic status of Odontophrynus americanus. Canadian Journal of Genetics and Cytology, 9, 531–542.

    Bogart, J.P. & Wasserman, A.O. (1972) Diploid-ployploid cryptic species pairs: a possible clue to evolution by polyploidization in anuran amphibians. Cytogenetics, 11, 7–24.

    Borteiro, C., Cruz, J.C., Kolenc, F. & Aramburu, A. (2009) Chytridiomycosis in frogs from Uruguay. Diseases of Aquatic Organisms, 84, 159–162.

    Brandão, R.A. & Batista, C.G. (2000) Descrição do girino de Odontophrynus salvatori (Anura, Leptodactylidae). Iheringia (Zoologia), 89, 165–170.

    Broyles, R.H. (1981) Changes in the blood during amphibian metamorphosis. In: Gilbert, L.I. & Frieden, E. (Eds.), Metamorphosis, a Problem in Developmental Biology. Plenum Press, New York, pp. 461–490.

    Castellano, S., Rosso, A., Doglio, S. & Giacoma, C. (1999) Body size and calling variation in the green toad (Bufo viridis). Journal of Zoology, 248, 83–90.

    Cei, J.M. (1980) Amphibians of Argentina. Monitore Zoologico Italiano, N.S., Monografia, 2, 1–609.

    Cei, J.M. (1987) Additional notes to “Amphibians of Argentina”: an update, 1980–1986. Monitore Zoologico Italiano, N.S., Monografia, 21, 209–272.

    Cei, J.M. & Crespo, E.G. (1982) Differences in larval morphology of allopatric isolated populations of the Odontophrynus occidentalis group from western Argentina. Arquivos do Museu Bocage, Sér. A, 1, 335–340.

    Cianciarullo, A.M., Soares, M.J. & Beçak, W. (2000) Erythropoiesis in the diploid and tetraploid Odontophrynus americanus: an evolutionary approach in these cryptic species (Amphibia, Anura, Leptodactylidae). Comparative Haematology International, 10, 19–29.

    Colombo, P., Kindel, A., Vinciprova, G. & Krause, L. (2008) Composição e amenaças á conservação dos anfíbios anuros do Parque Estadual de Itapeva, Município de Torres, Rio Grande do Sul, Brasil. Biota Neotropica, 8, 229–240.

    de Almeida, T.M.B., Ruiz, I.R.G. & Beçak, W. (1986) Ribosomal gene activity detected by silver staining in two diploid populations of Odontophrynus americanus (Amphibia, Anura) from southern Brazil. Revista Brasileira de Genetica, 9, 433–437.

    de Barale, G.D., María, G.A., di Tada, I.E. & Lisanti, J.A. (1981) Presencia de Odontophrynus americanus (Anura, Leptodactylidae) tetraploide en la Provincia de Córdoba. Revista de la Universidad Nacional de Río Cuarto, 1, 121–125.

    Echeverría, D.D. & Filipello, A.M. (1991[1994]) Consideraciones acerca de la fórmula dentaria y del aparato bucal de las larvas de Odontophrynus occidentalis (Berg, 1896) (Anura, Leptodactylidae). Physis B, 49, 59–63.

    Echeverría, D.D. & Montanelli, S.B. (1992[1995]) Acerca del aparato bucal y de las fórmulas dentarias en Odontophrynus americanus (Duméril y Bibron, 1841) (Anura, Leptodactylidae). Physis B, 50, 37–43.

    Eikhorst, R. (1988) Die Verteilung von diploiden und triploiden Larven des Teichfrosches Rana esculenta Linnaeus, 1758 in einer reinen Bastardpopulation (Anura: Ranidae). Salamandra, 24, 59–68.

    Fernández, K. & Fernández, M. (1921) Sobre la biología y reproducción de algunos batracios argentinos. Anales de la Sociedad Científica Argentina, 91, 97–140.

    Frost, D.R. (2010) Amphibian Species of the World: an Online Reference Version 5.4, American Museum of Natural History, New York, USA. Available from http://research.amnh.org/herpetology/amphibia/index.php (accessed 5 May 2010)

    Gallardo, J.M., Echeverría, D.D., Montanelli, S.B. & Fiorito de López, L. (1990[1991]) Microanatomía del aparato bucal de las larvas de anuros de la fauna argentina. I) larvas de los alrededores de Buenos Aires. Boletín de la Asociación Herpetológica Argentina, 4, 2–3.

    Gosner, K.L. (1960) A simplified table for staging anuran embryos and larvae with notes on identification. Herpetologica, 16, 183–190.

    Green, D.M. & Sessions, S.K. (1991) Nomenclature for chromosomes. In: Green, D.M. & Sessions, S.K. (Eds.), Amphibian Cytogenetics and Evolution. Academic Press, San Diego, USA, pp. 431–432.

    Grenat, P.R., Bionda, C., Salas, N.E. & Martino, A.L. (2009a) Variation in red blood cells size between juveniles and adults of Odontophrynus americanus. Amphibia-Reptilia, 30, 141–145.

    Grenat, P., Salas, N.E. & Martino, A.L. (2009b) Erythrocyte size as diagnostic character for the identification of live cryptic Odontophrynus americanus and O. cordobae (Anura: Cycloramphidae). Zootaxa, 2049, 67–68.

    Grenat, P.R., Zavala Gallo, L.M., Salas, N.E. & Martino, A.L. (2009c) The tadpole of Odontophrynus cordobae Martino & Sinsch, 2002 (Anura: Cycloramphidae) from central Argentina. Zootaxa, 2151, 66–68.

    Heyer, W.R., Rand, A.S., Gonçalves da Cruz, C.A., Peixoto, O.L. & Nelson, C.E. (1990) Frogs of Boracéia. Arquivos de Zoologia, 31, 237–410.

    Holloway, A.K, Canatella, D.C., Gerhardt, H.C. & Hillis, D.M. (2006) Polyploids with different origins and ancestors from a single sexual polyploid species. The American Naturalist, 167, 88–101.

    Howell, W.M. & Black, D.A. (1980) Controlled silver-staining of nucleolus organizer regions with a protective colloidal developer: 1–step method. Experentia, 36, 1014–1015.

    Keller, M.J. & Gerhardt, H.C. (2001) Polyploidy alters advertisement call structure in gray tree frogs. Proceedings of the Royal Society of London B, 268, 341–345.

    Kolenc, F., Borteiro, C., Baldo, D., Ferraro, D.P. & Prigioni, C. (2009) The tadpoles and advertisement calls of Pleurodema bibroni Tschudi and Pleurodema kriegi (Müller), with notes on their geographic distribution and conservation status (Amphibia, Anura, Leiuperidae). Zootaxa, 1969, 1–35.

    Lannoo, M.J. (1987) Neuromast topography in anuran amphibians. Journal of Morphology, 191, 115–129.

    Lavilla, E.O. & Scrocchi, G.J. (1986) Morfometría larval de los géneros de Telmatobiinae (Anura: Leptodactylidae) de Argentina y Chile. Physis, 44, 39–43.

    Levan, A., Fredga, K. & Sandberg, A. (1964) Nomenclature for centromeric position on chromosomes. Hereditas, 52, 201–220.

    Martino, A.L. & Sinsch, U. (2002) Speciation by polyploidy in Odontophrynus americanus. Journal of Zoology (London), 257, 67–81.

    McDiarmid, R.W. & Altig, R. (1999) Research. Materials and Techniques. In: McDiarmid, R.W. & Altig, R. (Eds.), Tadpoles: The Biology of Anuran Larvae. The University of Chicago Press, Chicago, Illinois, USA, pp. 7–23.

    Mercadal, I.T. (1981) Determinación del nivel de ploidía en ejemplares preservados del género Ceratophrys. Amphibia-Reptilia, 1, 205–212.

    Montanelli, S.B. & Echeverría, D.D. (1990[1991]) Tres fórmulas dentarias en las larvas de Odontophrynus americanus (Duméril y Bibron, 1841) (Anura, Leptodactylidae). Boletín de la Asociación Herpetológica Argentina, 6, 3.

    Pereyra, M.O., Martí, D.A., Lescano, J.N., Rosset, S.D. & Baldo, D. (2009) Natural interspecific hybridization in Odontophrynus (Anura: Cycloramphidae). Amphibia-Reptilia, 30, 571–575.

    Polls-Pelaz, M. & Graf, J.D. (1988) Red blood cells size as an indicator of ploidy level in Rana kl. esculenta before and after metamorphosis. Alytes, 7, 53–61.

    Reeves, A. & Tear, J. (2000) Micromeasure version 3.3. Available from http://www.colostate.edu/Depts/Biology/MicroMeasure.

    Rosset, S. (2008) New species of Odontophrynus Reinhardt and Lütken 1862 (Anura: Neobatrachia) from Brazil and Uruguay. Journal of Herpetology, 42, 134–144.

    Rosset, S., Baldo, D., Lanzone, C. & Basso, N.G. (2006) Review of the geographic distribution of diploid and tetraploid populations of the Odontophrynus americanus species complex (Anura: Leptodactylidae). Journal of Herpetology, 40, 466–478.

    Rosset, S., Ferraro, D.P., Alcalde, L. & Basso, N.G. (2007) A revision of Odontophrynus barrioi (Anura: Neobatrachia): morphology, osteology, vocalizations, and geographic distribution. South American Journal of Herpetology, 2, 97–106.

    Ruiz, I.R.G., Cei, J.M. & Beçak, W. (1982) Chromosomal evolution in allopatric populations of the Odontophrynus occidentalis group (Amphibia, Anura) from western Argentina. Cytogenetics and Cell Genetics, 33, 303–311.

    Ruiz, I.R.G., de Almeida, T.M.B. & Beçak, W. (1984) Active nucleolar-organizer regions in polyploid populations of Odontophrynus americanus (Amphibia, Anura) from south Brazil. Genetica, 63, 31–37.

    Ruiz, I.R.G., Soma, M. & Beçak, W. (1981) Nucleolar organizer regions and constitutive heterochromatin in polyploid species of the genus Odontophrynus (Amphibia, Anura). Cytogenetics and Cell Genetics, 29, 84–98.

    Salas, N.E. (2006) Análisis cromosómico de Odontophrynus americanus, O. achalensis, O. cordobae y O. occidentalis (Anura: Leptodactylidae) de la Provincia de Córdoba, Argentina. Revista Española de Herpetología, 20, 31–38.

    Salas, N.E., Steinlein, C. & Schmid, M. (2000) Karyotype of Odontophrynus achalensis (Anura, Leptodactylidae). Amphibia-Reptilia, 21, 495–498.

    Salas, N.E., Zavattieri, M.V., di Tada, I.E., Martino, A.L. & Bridarolli, M.E. (1998) Bioacustical and etho-ecological features in amphibian communities of southern Córdoba Province (Argentina). Cuadernos de Herpetología, 12, 37–46.

    Salas, N.E. & di Tada, I.E. (1994) Análisis bioacústico del canto nupcial de Odontophrynus occidentalis y Odontophrynus achalensis (Anura: Leptodactylidae) en la Provincia de Córdoba. Revista de la Universidad Nacional de Río Cuarto, 14, 55–63.

    Salas, N.E. & di Tada, I.E. (1996) Análisis bioacústico en poblaciones tetraploides de Odontophrynus americanus (Anura: Leptodactylidae) en el Departamento de Río Cuarto, Provincia de Córdoba, Argentina. Revista de la Universidad Nacional de Río Cuarto, 16, 123–128.

    Savage, J.M. & Cei, J.M. (1965) A review of the leptodactylid frog genus Odontophrynus. Herpetologica, 21, 178–195.

    Schmid, M. (1978) Chromosome Banding in Amphibia. I. Constitutive heterochromatin and nucleolus organizer regions in Bufo and Hyla. Chromosoma, 66, 361–388.

    Schmid, M., Haaf, T. & Schempp, W. (1985) Chromosome banding in Amphibia IX. The polyploid karyotypes of Odontophrynus americanus and Ceratophrys ornata (Anura, Leptodactylidae). Chromosoma, 91, 172–184.

    Scott-Birabén, M.I. & Fernández-Marcinowski, K. (1921) Variaciones locales de caracteres específicos en larvas de anfibios. Anales de la Sociedad Científica Argentina, 22, 129–142.

    Stöck, M. (1998) Mating call differences between diploid and tetraploid green toads (Bufo viridis complex) in middle Asia. Amphibia-Reptilia, 19, 29–42.

    Straneck R., de Olmedo, E.V. & Carrizo, G.R. (1993) Catálogo de Voces de Anfibios Argentinos. Parte 1. Editorial L.O.L.A., Buenos Aires, Argentina, 131 pp.

    Sumner, A.T. (1972) A simple technique for demonstrating centromeric heterochromatin. Experimental Cell Research, 75, 304–306.

    White, M.J.D. (1954) Animal Cytology and Evolution. Cambridge University Press, Cambridge, England, 188 pp.