Abstract
Nematocysts (stinging organelles) are an important part of cnidarian identification, but the cnidomes of many species remain poorly known because of identification difficulties and confusing nomenclature. Currently, the cnidomes of 20 cubozoan species are documented in whole or in part (including four undescribed species and five newly described species); 18 of these are re-examined in detail, and the cnidomes of 13 species are newly presented. Type material was used for these studies where possible. A new category of nematocyst is designated, namely ‘p-rhopaloid’, characterized by having a v-shaped notch in the undischarged shaft, as in p-mastigophores, but having a lobed discharged shaft, as in rhopaloids; this type of nematocyst is found in both the Carybdeida and Chirodropida. Measurements, shape descriptions, and photographs are presented for all cnidomes studied. Also presented are a glossary to nematocyst terminology, descriptive sections on nematocyst nomenclature and preparation, and a key to the nematocysts of theCubozoa. Special remarks sections for taxonomists and forensic specialists are given, along with a summary of phylogenetic patterns. This catalogue will be useful to taxonomists, envenomation physicians, and toxinologists, as well as anyone wishing to identify cubozoan specimens or stings.References
Acuna, F.H. & Zamponi, M.O. (1997) The use of cnidocysts for ecological races identification from sea anemones populations (Anthozoa, Actiniidae). Iheringia Serie Zoologia, 82, 9–18.
Arneson, A.C. (1976). Life history of Carybdea alata (Reynaud, 1831). (Univ. Puerto Rico: Mayaguez). MS thesis. 43 pp., 2 pls.
Arneson, A.C. & Cutress, C.E. (1976) Life history of Carybdea alata Reynaud, 1831 (Cubomedusae). In: Mackie, G.O. (Ed.), Coelenterate Ecology and Behavior. Plenum Press, New York, 227–236.
Avian, M., Budri, N. & Rottini-Sandrini, L. (1997) The nematocysts of Carybdea marsupialis Linnaeus, 1758 (Cubozoa). In: den Hartog, J.C. (Ed.), Proc. 6th Int’l Conference on Coelenterate Biology. Nationaal Natuurhistorisch Museum, Leiden, The Netherlands, The Leeuwenhorst, Noordwijkerhout, The Netherlands, 21–26.
Berger, E.W. (1900) Physiology and histology of the Cubomedusae, including Dr. F.S. Conant’s notes on the physiology. Memoirs from the Biological Laboratory of the Johns Hopkins University, 4, 1–84.
Bloom, D.A., Burnett, J.W. & Alderslade, P. (1998) Partial purification of box jellyfish (Chironex fleckeri) nematocyst venom isolated at the beachside. Toxicon, 36, 1075–1085.
Bouillon, J. & Boero, F. (2000) Phylogeny and classification of Hydroidomedusae. Thalassia Salentina, 24, 1–296.
Calder, D.R. (1974) Nematocysts of the coronate scyphomedusa, Linuche unguiculata, with a brief reexamination of scyphozoan nematocyst classification. Chesapeake Science, 15, 170–173.
Calder, D.R. & Peters, E.C. (1975) Nematocysts of Chiropsalmus quadrumanus with comments on the systematic status of the Cubomedusae. Helgolaender wissenschaftliche Meeresuntersuchungen, 27, 364–369.
Campbell, S. (2005) Box jelly alert. Cairns Post, 29 November 2005, 1–2.
Carrette, T., Alderslade, P. & Seymour, J. (2002) Nematocyst ratio and prey in two Australian cubomedusans, Chironex fleckeri and Chiropsalmus sp. Toxicon, 40, 1547–1551.
Chapman, D.M. (1978) Microanatomy of the cubopolyp, Tripedalia cystophora (Class Cubozoa). Helgolaender wissenschaftliche Meeresuntersuchungen, 31, 128–168.
Cleland, J.B. & Southcott, R.V. (1965) Injuries to Man from Marine Invertebrates in the Australian Region. Commonwealth of Australia, Canberra, 282 pp.
Cornelius, P.F.S., Fenner, P.J. & Hore, R. (2005) Chiropsalmus maculatus sp. nov., a cubomedusa from the Great Barrier Reef. Memoirs of the Queensland Museum, 51, 399–405.
Currie, B., McKinnon, M., Whelan, B. & Alderslade, P. (2002) The Gove chirodropid: a box jellyfish appearing in the "safe season" [letter]. Medical Journal of Australia, 177, 649.
Currie, B. & Wood, Y.K. (1995) Identification of Chironex fleckeri envenomation by nematocyst recovery from skin. Medical Journal of Australia, 162, 478–480.
Cutress, C.E. & Studebaker, J.P. (1973) Development of the Cubomedusae, Carybdea marsupialis. Proceedings of the Association of Island Marine Laboratories of the Caribbean, 9, 25.
Endean, T.R. & Rifkin, J. (1975) Isolation of different types of nematocyst from the cubomedusan Chironex fleckeri. Toxicon, 13, 375–376.
Fenner, P.J., Fitzpatrick, P.F., Hartwick, R.J. & Skinner, R. (1985) "Morbakka", another cubomedusan. Medical Journal of Australia, 143, 550–555 + 536.
Gershwin, L. (2005a). Taxonomy and phylogeny of Australian Cubozoa. (James Cook University: Townsville, Queensland). PhD thesis. 221 pp., 49 plates.
Gershwin, L. (2005b) Two new species of jellyfishes (Cnidaria: Cubozoa: Carybdeida) from tropical Western Australia, presumed to cause Irukandji Syndrome. Zootaxa, 1084, 1–30.
Gershwin, L. (2005c) Carybdea alata auct. and Manokia stiasnyi, reclassification to a new family with description of a new genus and two new species. Memoirs of the Queensland Museum, 51, 501–523.
Gershwin, L. (In review) Comments on Chiropsalmus (Cnidaria: Cubozoa: Chirodropida): a preliminary revision of the Chiropsalmidae, with descriptions of two new species. Zootaxa.
Gershwin, L. & Alderslade, P. (2005) A new genus and species of box jellyfish (Cubozoa: Carybdeida) from tropical Australian waters. The Beagle, 21, 27–36.
Gravier-Bonnet, N. (1987) Nematocysts as taxonomic discriminators in thecate hydroids. In: Bouillon, J., Boero, F., Cicogna, F. & Cornelius, P.F.S. (Ed.), Modern Trends in the Systematics, Ecology, and Evolution of Hydroids and Hydromedusae. Clarendon Press, Oxford, 43–55.
Halstead, B.W. (1965) Poisonous and venomous marine animals of the world. Vol. 1 — Invertebrates. U.S. Government Printing Office, Washington D.C., 994 pp.
Hartwick, R. (unpublished) Nematocyst Identification Poster. James Cook University. Townsville, Queensland.
Hartwick, R.F. (1991) Observations on the anatomy, behaviour, reproduction and life cycle of the cubozoan Carybdea sivickisi. Hydrobiologia, 216/217, 171–179.
Hidaka, M. (1992) Use of nematocyst morphology for taxonomy of some related species of scleractinian corals. Galaxea, 11, 21–28 [Not seen].
Huynh, T.T., Seymour, J., Pereira, P., Mulcahy, R., Cullen, P., Carrette, T. & Little, M. (2003) Severity of Irukandji syndrome and nematocyst identification from skin scrapings. Medical Journal of Australia, 178, 38–41.
Kingston, C.W. & Southcott, R.V. (1960) Skin histiopathy in fatal jellyfish stinging. Transactions of the Royal Society of Tropical Medicine and Hygiene, 54, 373–384.
Kinsey, B.E. (1986) Barnes on Box Jellyfish. Sir George Fisher Centre for Tropical Marine Studies, James Cook University, Townsville, 76 pp., plus Appendix of 7 tape transcripts, 87 pp.
Little, M., Mulcahy, R.F. & Wenck, D.J. (2001) Life-threatening cardiac failure in a healthy young female with Irukandji syndrome. Anaesthesia and Intensive Care, 29, 178–180.
Little, M. & Seymour, J. (2003) Another cause of “Irukandji stingings”. Medical Journal of Australia, 179, 654.
Mariscal, R.N. (1971) Effect of a disulfide reducing agent on the nematocyst capsules from some coelenterates, with an illustrated key to nematocyst classification. In: Lenhoff, H.M., Muscatine, L. & Davis, L.V. (Ed.), Experimental Coelenterate Biology. University of Hawaii Press, Honolulu, 157–168.
Marques, A.C., Morandini, A.C. & Pinto, M.M. (1997) Cnidome of Chiropsalmus quadrumanus (Cnidaria, Cubozoa) from Brazil. Boletim de Resumos Expandidos VII COLACMAR, 136–138.
Matsumoto, G.I. (1995) Observations on the anatomy and behaviour of the cubozoan Carybdea rastonii Haacke. Marine & Freshwater Behaviour and Physiology, 26, 139–148.
Moore, S.J. (1988) A new species of cubomedusan (Cubozoa: Cnidaria) from northern Australia. The Beagle, Records of the Northern Territory Museum of Arts and Sciences, 5, 1–4.
Mulcahy, R. (1999) A severe case of Irukandji syndrome. In: (Ed.), Neurological Emergencies Handbook, Winter Symposium. Australasian College for Emergency Medicine, Melbourne, 88.
Oba, A., Hidaka, M. & Iwanaga, S. (2004) Nematocyst composition of the cubomedusan Chiropsalmus quadrigatus changes with growth. Hydrobiologia, 530/531, 173–177.
Okada, Y.K. (1927) Note sur l’ontogénie de Carybdea rastonii Haacke. Bulletin Biologique de la France, Tome 61, 241–249.
O’Reilly, G.M., Isbister, G.K., Lawrie, P.M., Treston, G.T. & Currie, B.J. (2001) Prospective study of jellyfish stings from tropical Australia, including the major box jellyfish Chironex fleckeri. Medical Journal of Australia, 175, 652–655.
Östman, C. (1983). Taxonomy of Scandinavian hydroids (Cnidaria, Campanulariidae): A study based on nematocyst morphology and isoenzymes. (Acta Universitatis Upsaliensis: Uppsala). PhD thesis. 22 pp.
Östman, C. (2000) A guideline to nematocyst nomenclature and classification, and some notes on the systematic value of nematocysts. Scientia Marina, 64, 31–46.
Östman, C., Piraino, S. & Roca, I. (1987) Nematocyst comparisons between some Mediterranean and Scandinavian campanulariids (Cnidaria, Hydrozoa). In: Bouillon, J., Boero, F., Cicogna, F. & Cornelius, P.F.S. (Ed.), Modern Trends in the Systematics, Ecology, and Evolution of Hydroids and Hydromedusae. Clarendon Press, Oxford, 299–310.
Rifkin, J. & Endean, R. (1983) The structure and function of the nematocysts of Chironex fleckeri Southcott, 1956. Cell & Tissue Research, 233, 563–577.
Schnadig, V.J., Burke, S.S., Bengston, K., Davie, K.D. & Nichols, M.M. (1991) Cytologic identification of jellyfish nematocysts in skin scraping. Acta Cytologica, 35, 629 [Abstract].
Shostak, S. (1996) Cnidocyst database, Available from: http://www.pitt.edu/sshostak/cnidocyst_database/cubozoa.html (accessed 10 December 2004)
Southcott, R.V. (1967) Revision of some Carybdeidae (Scyphozoa: Cubomedusae), including a description of the jellyfish responsible for the "Irukandji syndrome". Australian Journal of Zoology, 15, 651–671.
Stangl, K., Salvini-Plawen, L.v. & Holstein, T.W. (2002) Staging and induction of medusa metamorphosis in Carybdea marsupialis (Cnidaria, Cubozoa). Life & Environment, 52, 131–140.
Studebaker, J.P. (1972). Development of the cubomedusa, Carybdea marsupialis. (University of Puerto Rico: Mayaguez). MSc thesis. 52pp, 4 pls.
Weill, R. (1934) Contribution à l’étude des Cnidaires et de leurs Nématocystes. 2. Valeur taxonomique du cnidome. Travaux de la Station Zoologique de Wimereux, 11, 349–701.
Werner, B. (1975) Bau und Lebensgeschichte des Polypen von Tripedalia cystophora (Cubozoa, class nov., Carybdeidae) und seine Bedeutung für die Evolution der Cnidaria. Helgoländer wissenschaftliche Meeresuntersuchungen, 27, 461–504.
Werner, B. (1984) Klasse Cubozoa. In: Gruner, H.E. (Ed.), Lehrbuch der Speziellen Zoologie. Gustav Fischer Verlag, Stuttgart, 106–133.
Williamson, J., Fenner, P., Burnett, J. & Rifkin, J. (1996) Venomous and poisonous marine animals: a medical and biological handbook. NSW University Press, Sydney, Australia, 504 pp.
Wiltshire, C.J., Sutherland, S.K., Fenner, P.J. & Young, A.R. (2000) Optimization and preliminary characterization of venom isolated from 3 medically important jellyfish: the box (Chironex fleckeri), Irukandji (Carukia barnesi), and blubber (Catostylus mosaicus) jellyfish. Wilderness & Environmental Medicine, 11, 241–250.
Yamaguchi, M. & Hartwick, R. (1980) Early life history of the sea wasp, Chironex fleckeri (Class Cubozoa). In: Tardent, P. & Tardent, R. (Ed.), Developmental and Cellular Biology of Coelenterates. Elsvier/North Holland Biomedical Press, 11–16.
Yanagihara, A.A., Kuroiwa, J.M.Y., Oliver, L.M., Chung, J.J. & Kunkel, D.D. (2002) Ultrastructure of a novel eurytele nematocyst of Carybdea alata Reynaud (Cubozoa, Cnidaria). Cell & Tissue Research, 308, 307–318.
Zamponi, M.O. & Genzano, G.N. (1990) The use of nematocysts for identification of the common medusa-stage of the genus Obelia Peron & Lesueur, 1810 (Leptomedusae, Campanulariidae) from the subantarctic region. Plankton Newsletter, 13, 21–23.