Abstract
Based on morphology, distribution, and on the presence of a novel diploid number (2n = 30 chromosomes), a new species of frog Alsodes norae from the Coastal Range, Valdivia Province temperate Nothofagus forest, Southern Chile, is described. Hypothetical chromosomal rearrangements and the origin of its unusual karyotype are discussed. Among the South American frogs, the genus Alsodes is recognized by its complex relationships, and based on karyological and geographical distribution information its species are grouped in two larger groups, herein: the Andean group which is conservative, and the Coastal group which is far more variable in gross chromosome morphology. Additionally, the inclusion of Alsodes in the new family Cycloramphidae is commented.References
Bogart, J.P. (1970) Systematic problems in the amphibian family Leptodactylidae (Anura) as indicated by karyotypic analysis. Cytogenetics, 9, 369-383.
Bogart, J.P. (1991) The influence of life history on karyotypic evolution in frogs. In: Green D. & Sessions S. (Eds). Amphibian Cytogenetics and Evolution. San Diego, Academic Press. pp. 233–255.
Cei, J.M. (1980) Amphibians of Argentina. Monitore Zoologico Italiano Monographs, 2, 1–609.
Cherry, L.M., Case, S.M. & Wilson, A.C. (1978) Frog perspective on the morphological difference between humans and chimpanzees. Science, 200, 209–211.
Cuevas, C.C. & Formas, J.R. (1996) Heteromorphic sex chromosomes in Eupsophus insularis (Amphibia, Anura, Leptodactylidae). Chromosome Research, 4, 467–470.
Cuevas, C.C. & Formas, J.R. (2001) A new species of Alsodes (Amphibia, Anura, Leptodactylidae) from Central Chile. Amphibia-Reptilia, 22, 187–198.
Cuevas, C.C. & Formas, J.R. (2003) Cytogenetic analysis of four species of the genus Alsodes (Anura, Leptodactylidae) with comments about the karyological evolution of the genus. Hereditas, 138, 138–146.
Cuevas, C.C. & Formas, J.R. (2005a) Novel diploid number and chromosomal formula in a South American frog of the genus Alsodes (Amphibia, Leptodactylidae). Italian Journal of Zoology, 72, 87–91.
Cuevas, C.C. & Formas, J.R. (2005b) A new frog of the genus Alsodes (Leptodactylidae) from the Tolhuaca National Park, Andes Range, southern Chile. Amphibia-Reptilia, 26, 39–48
Díaz, N.F. (1989) Phenetic and phylogenetic relationships of the Chilean Alsodes and Telmatobius (Amphibia, Anura, Leptodactylidae) and proposal of a new genus. Studies on the Neotropical Fauna and Environment, 24, 25–33.
di Castri, F. (1968) Esquisse écologique du Chili. In: Delamare Deboutteville, C. & Rapoport, E., (Eds). Biologie de l’Amerique australe. Francais Argile C. R. Reun Etud. Paris, pp 7–82.
Formas, J.R. (1982) Sinonimia e identidad de la rana austral chilena Eupsophus vittatus (Philippi, 1902) (Anura, Leptodactylidae). Boletín Sociedad de Biología de Concepción, 60, 123–127.
Formas, J.R. & Vera, M.I. (1980) Karyological relationships among frogs of the genus Alsodes, with description of the karyotypes of A. vanzolinii and A. verrucosus. Copeia, 1983, 1101–1107.
Formas, J.R. & Vera, M.I. (1982) The status of two Chilean frogs of the genus Eupsophus (Anura, Leptodactylidae). Proceedings of the Biological Society of Washington, 95, 594–601.
Formas, J.R., Cuevas, C.C. & Brieva, L.M. (2002) A new species of Alsodes (Anura, Leptodactylidae) from Cerro Mirador, Cordillera Pelada, southern Chile. Proceedings of the Biological Society of Washington, 115, 708–719.
Formas, J.R., Cuevas, C.C. & Núñez, J. (1998) A new species of Alsodes (Amphibia, Anura, Leptodactylidae) from Southern Chile. Proceedings of the Biological Society of Washington, 111, 521–530.
Formas, J.R., Úbeda, C., Cuevas, C.C. & Núñez J. (1997) Alsodes australis, a new species of leptodactylid frog from the temperate Nothofagus forest of southern Chile and Argentina. Studies on Neotropical Fauna and Environment, 32, 200–211.
Formas, J.R., Cuevas, C.C. & Núñez, J. (2006) A new species of Telmatobius (Anura: Leptodactylidae) from Chusmisa, northern Chile. Herpetologica, 72, 173–183.
Frost, D., Grant, T., Faivovich, J., et al. (2006) The amphibian tree of life. Bulletin of the American Museum of Natural History, 297, 1–370.
Gallardo, J.M. (1965) A propósito de los Leptodactylidae (Amphibia, Anura). Papéis Avulsos do Departamento de Zoología de Sâo Paulo, 17, 77–87.
Green, D.M., Sessions S.K. (1991) Nomenclature for chromosomes. In: Green, D. and Sessions, S., (Eds). Amphibians Cytogenetics and Evolution. Academic Press, San Diego, pp. 431–432.
Ibarra-Vidal, H, Ortiz J.C. & Torres-Perez, F. (2004) Eupsophus septentrionalis n. sp. nueva especie de Leptodactylidae (Amphibia) de Chile Central. Boletín de la Sociedad de Biologia de Concepción, 75, 91–102
King, M. (1980) C-banding studies on Australian hylid frogs: Secondary constriction structure and the concept of euchromatin transformation. Chromosoma, 80, 191–217.
Kuramoto, M. (1990) A list of chromosome numbers of anurans amphibians. Bulletin of the Fukuoka University of Education, 39, 83–127.
Lande, R. (1979) Effective deme sizes during long-term evolution estimated from rates of chromosomal rearrangement. Evolution, 38, 743–752.
Lynch, J. (1978) A re-assessment of the telmatobiinae leptodactylid frogs of Patagonia. Occasional Paper Museum of Natural History University of Kansas, 72, 1–57.
Oberdorfer, E. (1960) Pflanzensoziologische Studien in Chile. Ein Vergleich mit Europa, Band II. In: Herausgegeben Von R. T. (Ed). Flora et Vegetatio Mundi. Weinheim Verlag Von J. Cramer. Berlin, pp. 1-208.
Rufas, J.S., Iturra, P., De Souza, W. & Sponda, P. (1982) Simple silver staining procedures for the location of nucleolus and nucleolar organizer under light and electron microscopy. Archivos de Biologia, 93, 267-274.
Stuart, B. L., R. F. Inger, & Voris, H. K. (2006) High level of cryptic species diversity revealed by sympatric lineages of Southeast Asian forest frogs. Biology Letters, 2, 470–474.
Sumner, A.T. (1972) A simple technique for demonstrating centromeric heterochromatin. Experimental Cell Research, 75, 304–306.
Veblen, T.T., Schlegel, F.M. & Oltremari, J.V. (1980) Temperate broad-leaved evergreen forest of South America. In: Ovington J. D. (Ed).Temperate broad-leaved evergreen forests. Elsevier Science Publishers, Printed in The Netherlands. Amsterdam, pp. 5–31.
Veloso, A.M., Iturra, P. & Galleguillos, G. (1979) Evidencias cromosómicas en el género Alsodes (Amphibia, Leptodactylidae), con la descripción de una nueva especie. Physis, 94, 91-98.
Veloso, A.M., Díaz, N.P., Iturra, P. & Penna, M. (1981) Descripción de una nueva especie de Telmatobino del género Alsodes (Amphibia, Leptodactylidae) de la Cordillera de Nahuelbuta (Sur de Chile). Medio Ambiente, 51, 72–77.
Veloso, A.M., Celis-Diez, J.L., Guerrero, P., Méndez, M., Iturra, P. & Simonetti, J. (2005) Description of a new Eupsophus species (Amphibia, Leptodactylidae) from the remnants of Maulino forest, Central Chile. Herpetological Journal, 15, 159–165.
Wiens, J.J. (1993) Systematic of the Leptodactylid frog genus Telmatobius in the Andes of Northern Perú. Occasional Paper Museum Natural History University of Kansas, 162, 1–76.