Abstract
The Dichroplini genera Scotussa, Leiotettix, Ronderosia, Atrachelacris, Chlorus, Eurotettix and Dichromatos have been grouped into the “Paranaense-Pampeano” informal genus group, based on characters of the male genitalia. However, recent molecular phylogenetic analyses showed weak support values or no support at all for this group. In this study, we used molecular and morphological characters to test the monophyly of this informal genus group. Morphological characters included aspects of the general morphology, and male and female genitalia as well. Whereas the molecular data was based on one mitochondrial gene: cytochrome oxidase I. Independent and combined phylogenetic analyses of the data were performed under both unweighted and implied weighting parsimony. Our results showed that, when only molecular data is considered, the “Paranaense-Pampeano” informal genus group is not recovered. However, the group is monophyletic according to morphological and combined analyses. The “Paranaense-Pampeano” informal genus group is considered to be a natural clade; therefore, we propose the genus group name Scotussae. As a final remark, the molecular data provided in most cases the same evidence of relationships as morphology.References
Amedegnato, C. (1977) Etude des Acridoidea centre et sud americains (Catantopinae sensu lato). Anatomie des genitalia, classification, repartition, phylogenie. These, Université de Paris, France.
Amedegnato, C., Chapco, W. & Litzenberger, G. 2003. Out of South America? Additional evidence of a southern origin of melanopline grasshoppers. Molecular Phylogenetics and Evolution 29:115–119.
Bensansson, D., Zhang, D., Hewitt, G.M. (2000) Frequent assimilation of mitochondrial DNA by grasshopper nuclear genomes. Molecular Biology and Evolution, 17, 406–415.
Carbonell, C.S., Cigliano, M.M. & Lange, C.E. (2006) Acridomorph (Orthoptera) species of Argentina and Uruguay. CD-ROM. Publications on Orthopteran Diversity. The Orthopterists’ Society at the Museo de La Plata.
Chapco, W. (2006) A note on the phylogenetic position of Duartettix montanus within the subfamily Melanoplinae. Journal of Orthoptera Research, 15, 59–63
Cigliano, M.M. & de Wysiecki, M.L. & Lange, C.E. (2000) Grasshopper (Orthoptera, Acridoidea) species diversity in the Pampas, Argentina. Journal of Diversity and Distributions 6, 81–93
Cigliano, M.M. (2007) Review of the South American genus Eurotettix Bruner (Orthoptera, Acridoidea, Melanoplinae). Systematic Entomology, 32, 176–195.
Cigliano, M.M. & Ronderos, R.A. (1994) Revision of the South American grasshopper genera Leiotettix Bruner and Scotussa Giglio Tos (Orthoptera, Acrididae, Melanoplinae). Transactions of the American Entomological Society, 120, 145–180.
Colombo, P., Cigliano, M, Sequeira, A., Lange, C., Vilardi, J. & Confalonieri, V.A. (2005) Molecular Phylogenetics of Dichoplus and related genera: karyotype diversification. Cladistics, 21, 375–381.
Eades, D.C. & Otte, D. (2009) Orthoptera Species File Online. Version 2.0/3.4. <http://Orthoptera.SpeciesFile.org>.
Goloboff, P.A. (1993) Estimating character weights during tree search. Cladistics, 9, 83–91.
Goloboff, P.A. (1997) Self-weighted optimization: Tree searches and character state reconstructions under implied transformation costs. Cladistics, 13, 225–245.
Goloboff, P.A., Farris, S. & Nixon, K (2003) Tree Analysis using New Technology. Published by the authors. http://www.cladistics.com/aboutTNT.html
Goloboff P.A, Farris, S., Källersjö, M., Oxelman, B., Ramírez, M.J. & Szumik C.A. (2003) Improvements to resampling measures of group support. Cladistics, 19, 324–332
Hillis, B., Mable, K. & Moritz, C. (Eds) Molecular systematics, 2nd.ed., Sinauer, Sunderland, Massachusetts, pp 407–514.
Huelsenbeck, J.P. & Ronquist, F. (2001) MrBayes: Bayesian inference of phylogenetic trees. Bioinformatics, 17, 754–755.
Kitching, I.A., Forey, P.L, Humphries, C.J. & Williams, D.M. (1998) Cladistics: The Theory and Practice of Parsimony Analysis. Oxford University Press Inc., New York, 252 pp.
Lunt, D.H., Zhand, D.X., Szymura, J.M. & Hewitt, G.M. (1996) The insect cytochrome oxidase I gene evolutionary patterns and conserved primers for phylogenetic studies. Insect MoleclarBiology, 5(3), 153–165.
Nixon, K.C. (2002) WinClada, Version 1.00.08. Published by the author, Ithaca, NY.
Normark, B.B. (1996) Phylogeny and evolution of parthenogenetic weevils of the Aramigus tessellatus species complex (Coleoptera: Curculionidae: Naupactini): Evidence from mitochondrial DNA sequences. Evolution, 50, 734–745.
Posada, D. & Crandall, K.A. (1998) Modeltest: testing the model of DNA substitution. Bioinformatics, 14, 817–818.
Reiss, R.A., Schwert, D.P. & Ashworth, A.C., (1995) Field preservation of Coleoptera for molecular genetics analyses. Environmental Entomology, 24, 716–719.
Ronderos, R.A. (1985) Consideraciones sobre la biogeografía de los Melanoplinae en Sudamérica (Orthoptera: Acrididae). Proceedings of the 3rd. Triennial Meeting of the Pan American Acridiological Society, 5–10 July 1981, pp. 53–54.
Ronquist, F. (1999) Phylogeny, classification and evolution of the Cynipoidea. Zoologica Scripta 28, 139–164.
Sorenson, M.D. & Fleischer, R.C. (1996) Multiple independent transposition of mitochondrial DNA control region sequences to the nucleus. Proceedings of the National Academy of Sciences of USA, 93, 15239–15243.
Scataglini, M. A., Lanteri, A. & Confalonieri, V.A. (2005) Phylogeny of the Pantomorus-Naupactus complex based on morphological and molecular data (Coleoptera: Curculionidae). Cladistics, 21, 131–142.
Swofford, D.L., Olsen, G.J., Waddell, P.J. & Hillis M. (1996) Phylogenetic inference. In: Zhang, D.M. & Hewitt, G.M. (eds.), Nuclear integrations: challenges for mitochondrial DNA markers. Trends in Ecology and Evolution 11, 247–251.
Zwickl, D.J. & Hillis, D.M. (2002) Increased taxon sampling greatly reduces phylogenetic error. Systematic Biology, 51, 588–598.