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Type: Articles
Published: 2012-11-30
Page range: 67–78
Abstract views: 292
PDF downloaded: 4

Redescription of Leptus kattikus Haitlinger, 2009 (Actinotrichida, Parasitengona, Erythraeidae) and molecular identification of its host from DNA barcoding

Institute of Biology, Department of Invertebrate Systematics and Ecology, Wrocław University of Environmental and Life Sciences, Kożuchowska 5b, 51-631 Wrocław, Poland
Institute of Biology, Department of Invertebrate Systematics and Ecology, Wrocław University of Environmental and Life Sciences, Kożuchowska 5b, 51-631 Wrocław, Poland
Institute of Biology, Department of Invertebrate Systematics and Ecology, Wrocław University of Environmental and Life Sciences, Kożuchowska 5b, 51-631 Wrocław, Poland
Institute of Biology, Department of Invertebrate Systematics and Ecology, Wrocław University of Environmental and Life Sciences, Kożuchowska 5b, 51-631 Wrocław, Poland
Acari Leptus taxonomy morphology host identification COI Phasmida

Abstract

A morphology-based redescription of Leptus kattikus Haitlinger, 2009, including re-examination of the holotype and supplemented with data of a series of specimens collected in Vietnam, is provided. Larvae at different levels of engorgement, all separated from one phasmid host, were examined. A polymerase chain reaction, conducted in order to amplify and sequence the DNA of parasitic larvae, resulted in molecular identification of the host species. This represents an alternative method of establishing the host identification for larvae which have already detached from the host prior to examination, and can therefore be employed in further studies of host spectrum.

References

  1. Boline Lassen, S., Nielsen, S.A., Skovgård, H. & Kristensen, M. (2011) Molecular identification of bloodmeals from biting midges (Diptera: Ceratopogonidae: Culicoides Latreille) in Denmark. Parasitology Research, 108, 823–829.

    Dabert, J., Ehrnsberger, R. & Dabert M. (2008) Glaucalgales tytonis sp. n. (Analgoidea, Xolalgidae) from the barn owl Tyto alba (Strigiformes, Tytonidea): compiling morphology with DNA barcode data for the taxon description in mites (Acari). Zootaxa, 1719, 41–52.

    Dabert, M., Witaliński, W., Kaźmierski, A., Olszanowski, Z. & Dabert, J. (2010) Molecular phylogeny of acariform mites (Acari, Arachnida): Strong conflict between phylogenetic signal and long-branch attraction artifacts. Molecular Phylogeny and Evolution, 56, 222–241.

    De Geer, C. (1778) ‘Mèmoires pour Servir a l’Histoire des Insectes.’ Vol. VIII (Stockholm), 1–950+49 Pl.

    Gabryś, G., Felska, M., Kłosińska, A., Staręga, W. & Mąkol, J. (2011) Harvestmen (Opiliones) as hosts of Parasitengona (Acari: Actinotrichida, Prostigmata) larvae. Journal of Arachnology, 39, 349–351.

    Haitlinger, R. (2000) Four new species of Leptus Latreille, 1796 (Acari, Prostigmata, Erythraeidae) from Central America. Systematic and Applied Acarology, 5, 131–142.

    Haitlinger, R. (2009) New records of mites (Acari: Prostigmata: Erythraeidae) from Nepal, with a description of Leptus (Leptus) kattikus sp. nov. Systematic and Applied Acarology, 14, 60–64.

    Humair, P.F., Douet, V., Morán Cadenas, F., Schouls, L.M., Van De Pol, I. & Gern, L. (2007) Molecular identification of bloodmeal source in Ixodes ricinus ticks using 12S rDNA as a genetic marker. Journal of Medical Entomology, 44, 869–80.

    Kneeland, S.C. & Rhymer, J.M. (2007) A molecular identification key for freshwater mussel glochidia encysted on naturally parasitized fish hosts in Maine, USA. Journal of Molluscan Studies, 73, 279–282.

    Łaydanowicz, J. & Mąkol, J. (2010) Correlation of heteromorphic life instars in terrestrial Parasitengona mites and its impact on taxonomy—the case of Leptus molochinus (C. L. Koch, 1837) and L. ignotus (Oudemans, 1903) (Acari: Actinotrichida: Prostigmata: Erythraeidae). Journal of Natural History, 44, 669–697.

    Mąkol, J. (2010) A redescription of Balaustium murorum (Hermann, 1804) (Acari: Prostigmata: Erythraeidae) with notes on related taxa. Annales Zoologici, 60, 439–454.

    Mąkol, J. & Felska, M. (2011) New records of spiders (Araneae) as hosts of terrestrial Parasitengona mites (Acari: Actinotrichida, Prostigmata). Journal of Arachnology, 39, 352–354.

    Mąkol, J., Łaydanowicz, J. & Kłosińska, A. (2009) A redescription of Neothrombium neglectum (Bruyant, 1909) with revision of Neothrombium (Acari: Actinotrichida: Prostigmata: Neothrombiidae) and notes on the biology of species. International Journal of Acarology, 35, 275–301.

    Mąkol, J., Gabryś, G. & Łaydanowicz, J. (2011) Leptus phalangii (De Geer, 1778) (Acari: Actinotrichida: Prostigmata)—redescription, ecology and taxonomic notes on its relatives. Annales Zoologici, 61, 535–546.

    Mąkol, J., Kłosińska, A. & Łaydanowicz, J. (2012) Host–parasite interactions within terrestrial Parasitengona (Acari, Trombidiformes, Prostigmata). International Journal of Acarology, 38, 18–22.

    Mayoral, J.G. & Barranco, P. (2011) New species of larval Leptus (Parasitengona: Erythraeidae) and new records of larval Erythraeidae parasitizing Orthoptera from French Guiana. Acarologia, 51, 411–417.

    Murdock, C.C., Olival, K.J. & Perkins, S.L. (2010) Molecular Identification of Host Feeding Patterns of Snow-Melt Mosquitoes (Diptera: Culicidae): Potential Implications for the Transmission Ecology of Jamestown Canyon Virus.Journal of Medical Entomology, 47, 226–229.

    Southcott, R.V. (1961) Studies on the systematics and biology of the Erythraeoidea (Acarina), with a critical revision of the genera and subfamilies. Australian Journal of Zoology, 9, 367–610.

    Southcott, R.V. (1984) Acari from operation Drake in New Guinea. 4. A new species of Leptus (Erythraeidae). Acarologia, 25, 351–358.

    Southcott, R.V. (1992) Revision of the larvae of Leptus Latreille (Acarina, Erythraeidae) of Europe and North America, with descriptions of post-larval instars. Zoological Journal of the Linnean Society, 105, 1–153.

    Southcott, R.V. (1999) Larvae of Leptus (Acarina: Erythraeidae), free-living or ectoparasitic on arachnids and lower insects of Australia and Papua New Guinea, with description of reared post-larval instars. Zoological Journal of the Linnean Society, 127, 113–276.

    Swan, D.C. (1936) Berlese's Fluid: Remarks upon its preparation and use as a mounting medium. Bulletin of Entomological Research, 27, 389–391.

    Tamura, K., Dudley, J., Nei, M. & Kumar, S. (2007) MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Molecular Biology and Evolution, 24, 1596–1599.

    Walter, D.E. & Krantz, G.W. (2009) Collection, rearing, and preparing specimens. In: Krantz G.W. & Walter D.E. (Eds.) A manual of Acarology. 3rd edn. Texas Tech University Press, pp. 83–96.

    Wohltmann, A. (2000) The evolution of life histories in Parasitengona (Acari: Prostigmata). Acarologia, 41, 145–204.