Skip to main content Skip to main navigation menu Skip to site footer
Responsive image
Issue: Vol. 4083 No. 2: 19 Feb. 2016
Type: Correspondence
Published: 2016-02-19
DOI: 10.11646/zootaxa.4083.2.9
Page range: 297–300
Abstract views: 52
PDF downloaded: 37

The effect of two fixation methods (TAF and DESS) on morphometric parameters of Aphelenchoides ritzemabosi  

Research Institute of Horticulture, Konstytucji 3 Maja 1/3 Skierniewice, Poland. Museum and Institute of Zoology, Wilcza 64, 00-679 Warsaw, Poland.
Research Institute of Horticulture, Konstytucji 3 Maja 1/3 Skierniewice, Poland.
Museum and Institute of Zoology, Wilcza 64, 00-679 Warsaw, Poland.
Museum and Institute of Zoology, Wilcza 64, 00-679 Warsaw, Poland.
Nematoda Aphelenchoides ritzemabosi Poland

Abstract

Identification of nematode species by using conventional methods requires fixation of the isolated material and a suitable preparation for further analyses. Tentative identification using microscopic methods should also be performed prior to initiating molecular studies. In the literature, various methods are described for the preparation of nematodes from the genus Aphelenchoides for identification and microscopic studies. The most commonly used fixatives are formalin (Timm 1969; Szczygieł & Cid del Prado Vera 1981, Crozzoli et al. 2008, Khan et al. 2008), FAA (Wasilewska 1969; Vovlas et al. 2005, Khan et al. 2007) and TAF (Hooper 1958, Chizhov et al. 2006, Jagdale & Grewal 2006).

 

References

  1. Brown, D.J.F. & Topham, P.B. (1984) A comparison of reported variation in the morphometrics of Xiphinema diversicaudatum (Nematoda: Dorylaimida) and the effects of some methods of reparing specimens for examination by optical microscopy. Nematologia Meditteranea, 12, 169–186.

    Chizhov, V.N., Chumakova, O.A., Subbotin, S.A. & Baldwin, J.G. (2006) Morphological and molecular characterization of foliar nematodes of the genus Aphelenchoides: A. fragariae and A. ritzemabosi (Nematoda: Aphelenchoididae) from the Main Botanical Garden of Russian Academy of Science, Moscow. Russian Journal of Nematology, 14 (2), 179–184.

    Courtney, W.D., Polley, D. & Miller, V.L. (1955) TAF, an improved fixative in nematode technique. Plant Disease Reporter, 39, 570–571.

    Crozzoli, R., Hurtado, T., Perichi, G. & Arcia, A. (2008) Characterization of a Venezuelan population of Aphelenchoides ritzemabosi on chrysanthemum. Nematologia Meditteranea, 36, 79–83.

    Dobosz, R., Winiszewska, G., Malewski, T., Rybarczyk-Mydłowska, K., Tereba, A., Kowalewska, K., Gawlak, M. & Bogdanowicz, W. (2013) Morphological and molecular features of Punctodera stonei Brzeski, 1998 (Nematoda: Heteroderidae) – species associated with roots of grasses. Annales Zoologici, 63, 157–162.
    http://dx.doi.org/10.3161/000345413X669487

    Grewal, P.S., Richardson, P.N. & Wright, D.J. (1990) Effects of killing, fixing and mounting methods on taxonomic characters of parthenogenetic adult female Caenorhabditis elegans (Nematoda: Rhabditidae). Revue de Nématologie, 13 (4), 437–444.

    Hooper, D.J. (1958) Aphelenchoides dactylocercus n. sp. and A. sacchari n. sp. (Nematoda: Aphelenchoidea). Nematologica, 3, 229–235.
    http://dx.doi.org/10.1163/187529258X00454

    Jagdale, G.B. & Grewal, P.S. (2006) Infection behaviour and overwintering survival of foliar nematodes, Aphelenchoides fragariae, on Hosta. Journal of Nematology, 38 (1), 130–136.

    Jiang, L., Li, X. & Zheng, J. (2007) First record of Bursaphelenchus rainulfi on pine trees from eastern China and its phylogenetic relationship with intro-genus species. Journal of Zheijang University SCIENCE B, 8 (5), 345–351.
    http://dx.doi.org/10.1631/jzus.2007.B0345

    Khan, M.R., Handoo, Z.A., Rao, U., Rao, S.B. & Prasad, J.S. (2012) Observations on the foliar nematode, Aphelenchoides besseyi, infecting Tuberose and Rice in India. Journal of Nematology, 44 (4), 391–398.

    Khan, Z., Son, S.H., Moon, H.S., Kim, S.G., Shin, H.D., Jeon, Y.H. & Kim, Y.H. (2007) Description of foliar nematode, Aphelenchoides fragariae (Nematoda: Aphelenchida) with additional characteristics from Korea. Journal of Asia-Pacific Entomology, 10 (4), 313–315.
    http://dx.doi.org/10.1016/S1226-8615(08)60368-4

    Khan, Z., Son, S.H., Shin, H.D. & Kim, Y.H. (2008) First report of a foliar nematode Aphelenchoides fragariae (Aphelenchidae) on Stachys riederi var. japonica, a medicinal plant, in Korea. Plant Pathology Journal, 24 (1), 97–100.
    http://dx.doi.org/10.5423/PPJ.2008.24.1.097

    Lamberti, F. & Sher, S.A. (1969) A Comparison of preparation techniques in taxonomic studies of Longidorus africanus Merny. Journal of Nematology, 1 (3), 193–200.

    Luc, M., Sikora, R.A. & Bridge, J. (2005) Plant Parasitic Nematodes in Subtropical and Tropical Agriculture, CABI, 871 pp.
    http://dx.doi.org/10.1079/9780851997278.0000

    Naem, S., Pagan, C. & Nadler, S.A. (2010) Structural restoration of nematodes and acanthocephalans fixed in high percentage alcohol using DESS solution and rehydration. Journal of Parasitology, 96 (4), 809–811.
    http://dx.doi.org/10.1645/GE-2402.1

    Schwartz, M. (1911) Die Aphelenchen der Veilchengallen und der Blattflecken an Farnen und Chrysanthemum. Arbeiten aus der Kaiserlichen Biologischen Anstalt für Land- und Forstwirtschaft, 8, 303–344.

    Seinhorst, J.W. (1962) On the killing, fixation and transferring to glycerin of nematodes. Nematologica, 8 (1), 29–32.
    http://dx.doi.org/10.1163/187529262X00981

    Seinhorst, J.W. (1966) Killing nematodes for taxonomic study with hot F.A. 4:1. Nematologica, 12 (1), 178.
    http://dx.doi.org/10.1163/187529266x00239

    Steiner, G. & Buhrer, E.M. (1932) Miscellaneous notes on nemic diseases. Plant Disease Reporter, 16, 137.

    Strona, G., Stefani, F. & Galli, P. (2009) Field preservation of monogenean parasites for molecular and morphological analyses. Parasitology International, 58, 51–54.
    http://dx.doi.org/10.1016/j.parint.2008.10.001

    Szczygieł A. & Cid del Prado Vera, I. (1981) Association of Aphelenchoides fragariae and A. ritzemabosi with strawberry plants in Mexico. Zeszyty Problemowe Postępów Nauk Rolniczych, 249, 81–85.

    Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M. & Kumar, S. (2011) MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance and maximum parsimony methods. Molecular Biology and Evolution, 28, 2731–2739.
    http://dx.doi.org/10.1093/molbev/msr121

    Timm, R.W. (1969) Two marine species of Aphelenchoides. Nematologica, 15, 370–375.
    http://dx.doi.org/10.1163/187529269X00434

    Vovlas, N., Minuto, A., Garibaldi, A., Troccoli, A. & Lamberti, F. (2005) Identification and histopathology of the foliar nematode Aphelenchoides ritzemabosi (Nematoda: Aphelenchoididae) on basil in Italy. Nematology, 7 (2), 301–308.
    http://dx.doi.org/10.1163/1568541054879548

    Wasilewska, L. (1969) Aphelenchoides dubius sp. n. from Poland (Nematoda, Aphelenchoidea). Bulletin de L'Academie Polonaise des Sciences, Série des Sciences Biologiques, 17 (7), 455–458.

    Yoder, M., Tandingan De Ley, I., King, I.W., Mundo-Ocampo, M., Mann, J., Blaxter, M., Potras, L. & De Ley, P. (2006) DESS: a versatile solution for preserving morphology and extractable DNA of nematodes. Nematology, 8 (3), 367–376.
    http://dx.doi.org/10.1163/156854106778493448