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Article
Published: 2016-05-03

Phylogenetic relationships of geckos of the Hemiphyllodactylus harterti group, a new species from Penang Island, Peninsular Malaysia, and a likely case of true cryptic speciation

Department of Biology, La Sierra University, 4500 Riverwalk Parkway, Riverside, California 92515 USA.
Department of Biology, La Sierra University, 4500 Riverwalk Parkway, Riverside, California 92515 USA.
Department of Biology, Brigham Young University, 150 East Bulldog Boulevard, Provo, Utah 84602 USA.
School of Biological Sciences, Universiti Sains Malaysia, 11800 USM, Pulau Pinang, Penang, Malaysia.
School of Biological Sciences, Universiti Sains Malaysia, 11800 USM, Pulau Pinang, Penang, Malaysia. Center for Marine and Coastal Studies, Universiti Sains Malaysia, 11800 USM, Pulau Pinang, Malaysia.
School of Biological Sciences, Universiti Sains Malaysia, 11800 USM, Pulau Pinang, Penang, Malaysia.
Hemiphyllodactylus Malaysia Penang phylogeny new species cryptic speciation Reptilia

Abstract

An integrative taxonomic analysis based on the mitochondrial gene ND2 and its flanking tRNAs, morphology, and color pattern indicates that a newly discovered gecko described herein as Hemiphyllodactylus cicak sp. nov. from Penang Hill on the Island of Penang, Peninsular Malaysia is a member of the H. harterti group. Hemiphyllodactylus cicak sp. nov. is most closely related to the clade composed of the sister species H. harterti from Bukit Larut, Perak in the Bintang Mountain Range and H. bintik from Gunung Tebu, Terengganu from the Timur Mountain Range. These three allopatric species form a monophyletic group that extends approximately 270 km across three isolated mountain ranges in northern Peninsular Malaysia. The molecular analysis also indicates that H. titiwangsaensis from the Titiwangsa Mountain Range is composed of three genetically distinct allopatric populations. The southern two populations from Fraser’s Hill and Genting Highlands, Pahang have an uncorrected pairwise sequence divergence of 3.5% whereas these two populations have 12.4 and 12.8 % sequence divergences, respectively, from the northern population at Cameron Highlands, Pahang. Although the high sequence divergence clearly distinguishes the southern two populations from the former as a different species, all three populations are morphologically indistinguishable, leading to the hypothesis of a true, cryptic speciation event.

References

  1. Bickford, D., Lohman, D.J., Sodhi, N.S., Ng, P.K.L., Meier, R., Winker, K., Ingram, K.K. & Das, I. (2007) Cryptic species as a window on diversity and conservation. Trends in Ecology & Evolution, 22, 148–155.
    http://dx.doi.org/10.1016/j.tree.2006.11.004

    Grismer, L.L. (2011) Lizards of Peninsular Malaysia, Singapore and their Adjacent Archipelagoes. Their Description, Distribution, and Natural History. Frankfurt am Main, Edition Chimaira, 728 pp.

    Grismer, L.L., Wood Jr., P.L., Anuar, S., Muin, M.A., Quah, E.S.H., McGuire, J.A., Brown, R.A., Ngo, V.T. & Pham, H.T. (2013) Integrative taxonomy uncovers high levels of cryptic species diversity in Hemiphyllodactylus Bleeker, 1860 (Squamata: Gekkonidae) and the description of a new species from Peninsular Malaysia. Zoological Journal of the Linnean Society, 169, 849–880.
    http://dx.doi.org/10.1111/zoj.12064

    Grismer, L.L., Riyanto, A., Djoko, I.T. & McGuire, J.A. (2014) A new species of Hemiphyllodactylus Bleeker, 1860 (Squamata: Gekkonidae) from Pulau Enggano, southwestern Sumatra, Indonesia. Zootaxa, 3821 (4), 485–495
    http://dx.doi.org/10.11646/zootaxa.3821.4.7

    Grismer, L.L., Wood Jr., P.L. & Cota, M. (2014a) A new species of Hemiphyllodactylus Bleeker, 1860 (Squamata: Gekkonidae) from northwestern Thailand. Zootaxa, 3760 (1), 67–68.
    http://dx.doi.org/10.11646/zootaxa.3760.1.4

    Grismer, L.L., Wood Jr., P.L., Anuar, S., Riyanto, A., Ahmad, N., Muin, M.A., Sumontha, M., Grismer, J.L., Chan, K.O., Quah, E.S.H. & Pauwels, O.S.G. (2014b) Systematics and natural history of Southeast Asian Rock Geckos (genus Cnemaspis Strauch, 1887) with descriptions of eight new species from Malaysia, Thailand, and Indonesia. Zootaxa, 3880 (1), 1–147.

    http://dx.doi.org/10.11646/zootaxa.3880.1.1

    Grismer, L.L., Wood Jr., P.L., Anuar, S., Mohd., M.A., Quah, E.H., McGuire, J.A., Brown, R.M., Ngo, V.T. & Hong, P. (2014c) Integrative taxonomy uncovers high levels of cryptic species diversity in Hemiphyllodactylus Bleeker, 1860 (Squamata: Gekkonidae) and the description of a new species from Peninsular Malaysia. Zoological Journal of the Linnean Society, 169, 849–880.
    http://dx.doi.org/10.1111/zoj.12064

    Grismer, L.L., Wood Jr., P.L., Anuar, S., Quah, E.S.H., Muin, M.A., Chan, KO., Sumarli, A.X., & Loredo, A.I. (2015) Repeated evolution of sympatric, paleoendemic species in closely related, co –distributed lineages of Hemiphyllodactylus Bleeker, 1860 (Squamata: Gekkonidae) across a sky –island archipelago in Peninsular Malaysia. Zoological Journal of the Linnean Society, 174, 859–876.
    http://dx.doi.org/10.1111/zoj.12254

    Heinicke, M.P., Greenbaum, E., Jackman, T.R., & Bauer, A.M. (2011) Phylogeny of a trans-Wallacean radiation (Squamata, Gekkonidae, Gehyra) supports a single early colonization of Australia. Zoologica Scripta, 40, 584–602.
    http://dx.doi.org/10.1111/j.1463-6409.2011.00495.x

    Huelsenbeck, J.P. & Ronquist, F. (2001) MRBAYES: Bayesian inference of phylogeny. Bioinformatics, 17, 754−755. Available from: http://dx.doi.org/10.1093/bioinformatics/17.8.754 (accessed June 2015)

    Macey, J. & Schulte, J. (1999) Molecular phylogenetics, trna evolution, and historical biogeography in anguid lizards and related taxonomic families. Molecular Phylogenetics and Evolution, 12 (3), 250–272.
    http://dx.doi.org/10.1006/mpev.1999.0615

    Miller, M.A., Pfeiffer, W. & Schwartz, T. (2010) Creating the CIPRES Science Gateway for inference of large phylogenetic trees in Proceedings of the Gateway Computing Environments Workshop (GCE), New Orleans, LA, 1–8, Available from: https://www.phylo.org/portal2/login!input.action/ (accessed June 2015)

    Ngo, V.T., Grismer, L.L., Thai, P.H. & Wood Jr., P.L. (2014) A new species of Hemiphyllodactylus Bleeker, 1860 (Squamata: Gekkonidae) from Ba Na-Nui Nature reserve, central Vietnam, Zootaxa, 3760 (4), 539–552
    http://dx.doi.org/10.11646/zootaxa.3760.4.3

    Nguyen, T.Q., Lehmann, T., Le, M.D., Duong H.T., Bonkowski, M. & Ziegler, T. (2013) A new species of Hemiphyllodactylus (Reptilia: Gekkonidae) from northern Vietnam. Zootaxa, 3736 (1), 89–98.
    http://dx.doi.org/10.11646/zootaxa.3736.1.5

    Nguyen, T.Q., Botoc, A., Le, M.D., Nophaseud, L., Zug, G., Bonkowski, M. & Ziegler, T. (2014) A new species of Hemiphyllodactylus (Reptilia: Gekkonidae) from northern Laos. Zootaxa, 3827 (1), 45–56.
    http://dx.doi.org/10.11646/zootaxa.3827.1.4

    Rambaut, A., Suchard, M.A., Xie, D. & Drummond, A.J. (2014) Tracer v1.6. http://beast.bio.ed.ac.uk/Tracer

    Ronquist, F., Teslenko, M., Van der Mark, P., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology, 61, 539−542.

    http://dx.doi.org/10.1093/sysbio/sys029

    Sabaj-Pérez, M.H. (Ed) (2010) Standard symbolic codes for institutional resource collections in herpetology and ichthyology: An Online Reference, Version 2.0, American Society of Ichthyologists and Herpetologists, Washington, DC. Available from: http://www.asih.org

    Siler, C.D., Oaks, J.R., Esselstyn, J.A., Diesmos, A.C. & Brown, R.M. (2010) Phylogeny and biogeography of Philippine Bent–toed Geckos (Gekkonidae: Cyrtodactylus) contradict a prevailing model of Pleistocene diversification. Molecular Phylogenetics and Evolution, 55, 699–710.
    http://dx.doi.org/10.1016/j.ympev.2010.01.027

    Stamatakis (2014) RAxML Version 8: A tool for phylogenetic analysis and post–analysis of large phylogenies, Oxford Journals, Bioinformatics. Available from: http://bioinformatics.oxfordjournals.org/content/early/2014/01/21/bioinformatics.btu033.abstract? keytype=ref&ijkey=VTEqgUJYCDcf0kP/ (accessed June 2015)

    Tamura, K., Stecher, G., Peterson, D., Filipski, A. & Kumar, S. (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution, 30 (12), 2725–2729.

    http://dx.doi.org/10.1093/molbev/mst197

    Wilcox, T.P., Zwickl, D.L., Heath, T.A. & Hillis, D.M. (2002) Phylogenetic relationships of the Dwarf Boas and a comparison of Bayesian and bootstrap measures of phylogenetic support. Molecular Phylogenetics and Evolution, 25, 361–371.

    http://dx.doi.org/10.1016/S1055-7903(02)00244-0

    Zug, G.R. (2010) Speciation and dispersal in a low diversity taxon: The Slender geckos Hemiphyllodactylus (Reptilia, Gekkonidae). Smithsonian Contributions to Zoology, 631, 1–70.
    http://dx.doi.org/10.1016/S1055-7903(02)00244-0

How to Cite

COBOS, A., GRISMER, L. L., WOOD, JR., P. L., QUAH, E. S. H., ANUAR, S., & MUIN, M. A. (2016). Phylogenetic relationships of geckos of the <em>Hemiphyllodactylus harterti </em>group, a new species from Penang Island, Peninsular Malaysia, and a likely case of true cryptic speciation. Zootaxa, 4107(3), 367–380. https://doi.org/10.11646/zootaxa.4107.3.5