Skip to main content Skip to main navigation menu Skip to site footer
Responsive image
Issue: Vol. 4139 No. 1: 19 Jul. 2016
Type: Article
Published: 2016-07-19
DOI: 10.11646/zootaxa.4139.1.7
Page range: 117–127
Abstract views: 59
PDF downloaded: 1

Description of Cryptaphelenchus iranicus n. sp. (Nematoda: Ektaphelenchinae) recovered from bark samples of Pinus nigra from Iran 

Department of Plant Protection, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
Department of Plant Protection, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
Department of Nematology, Iranian Research Institute of Plant Protection, Tehran, Iran
Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, P.R. China
Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, P.R. China
Nematoda 28S D2/D3 fragment morphology new record new species phylogeny ribosomal DNA taxonomy

Abstract

Cryptaphelenchus iranicus n. sp., recovered from bark and wood samples of a weakened Pinus nigra in Kermanshah Province, western Iran, is described. The new species has females with body length of 250–330 µm and males 230–275 µm long, lip region set-off from body contour, 7–8 μm long stylet with small basal swellings, excretory pore located at 1.5–2.0 body diam. posterior to median bulb, post-vulval uterine sac short, 7–10 µm long and conical female posterior body end (tail) ending to a pointed tip. Males of the new species have seven (1+2+2+2) caudal papillae and a short mucro at tail tip. Based on morphological characters, the new species is close to C. cirrus, C. latus and C. leptocaudus. Phylogenetic analyses using the D2/D3 fragment of 28S rDNA show the new species forming a clade with other Cryptaphelenchus species with maximal (1.00) Bayesian posterior probability (BPP) in Bayesian inference and 100% bootstrap value (BS) in the maximum likelihood method. The Cryptaphelenchus clade forms a monophyletic group with members of subfamily Ektaphelenchinae.

 

References

  1. Aliramaji, F., Pourjam, E., Kargar, A., Atighi, M.R. & Pedram, M. (2015) Description of Ektaphelenchoides caspiensis n. sp. (Nematoda: Ektaphelenchinae) from Iran. Nematology, 17, 169–177.
    http://dx.doi.org/10.1163/15685411-00002862

    Aliramaji, F., Pourjam, E., Atighi, M.R., Ye, W., Roshan-Bakhsh, A. & Pedram, M. (2014a) Description of Ektaphelenchoides poinari sp. n. (Nematoda: Ektaphelenchinae) from Iran with compendium of the valid species of the genus Ektaphelenchoides Baujard, 1984. Russian Journal of Nematology, 22, 11–22.

    Aliramaji, F., Pourjam, E., Atighi, M.R., Kargar, A. & Pedram, M. (2014b) Devibursaphelenchus kheirii n. sp. (Nematoda: Ektaphelenchinae) from Iran with remarks on Devibursaphelenchus Kakuliya, 1967. Nematology, 16, 1069–1078.
    http://dx.doi.org/10.1163/15685411-00002833

    Atighi, M.R., Pourjam, E., Pedram, M., Ye, W. & Robbins, R.T. (2012) Molecular and morphological characterization of Ektaphelenchoides hunti sp. n. (Nematoda: Ektaphelenchinae) from northern Iran. Russian Journal of Nematology, 20 (1), 37–44.

    Atighi, M.R., Pourjam, E., Pedram, M., Ye, W., Robbins, R.T. & Namjou, S. (2013a) Molecular and morphological characterization of Ektaphelenchoides kelardashtensis sp. n. (Nematoda: Ektaphelenchinae) from northern Iran. Russian Journal of Nematology, 21 (1), 23–30.

    Atighi, M.R., Pourjam, F., Aliramaji, M., Ye, W. & Pedram, M. (2013b) Molecular and morphological characterization of Ektaphelenchoides andrassyi n. sp. (Nematoda: Ektaphelenchinae) from northern Iran. Journal of Nematode Morphology and Systematics, 16, 17–23.

    Bastian, H.C. (1865) Monograph on the Anguillulidae, or free nematoids, marine, land, and freshwater; with descriptions of 100 new species. Transactions of the Linnean Society of London, 25, 73–184.

    Baujard, P. (1984) Remarques sur la sous-famille des Ektaphelenchinae Paramonov, 1964 et proposition d’Ektaphelenchoides n. gen. (Nematoda: Aphelenchoididae). Revue de Nématologie, 7, 147–171.

    Braasch, H. (2009) Re-establishment of Devibursaphelenchus Kakuliya, 1967 (Nematoda, Aphelenchoididae) and proposal for a new combination of several Bursaphelenchus species. Journal of Nematode Morphology and Systematics, 12, 1–5.

    Castillo, P., Vovlas, N., Subbotin, S. & Troccoli, A. (2003) A new root-knot nematode, Meloidogyne baetica n. sp. (Nematoda: Heteroderidae), parasitizing wild olive in southern Spain. Phytopathology, 93, 1093–1102.
    http://dx.doi.org/10.1094/PHYTO.2003.93.9.1093

    Darriba, D., Taboada, G.L., Doallo, R. & Posada, D. (2012) jModelTest 2: more models, new heuristics and parallel computing. Nature Methods, 9, 772.
    http://dx.doi.org/10.1038/nmeth.2109

    De Grisse, A.T. (1969) Redescription ou modification de quelques techniques utilisées dans l’étude des nematodes phytoparasitaires. Mededelingen van de Rijksfaculteit der Landbouwwetenschappen Gent, 34, 351–369.

    Devdariani, Ts. G. (1974) [New nematode species from Monochamus sutor.] Soobschcheniya Akademii Nauk Gruzinskoi SSR, 76, 709–712. [in Russian]

    Esmaeili, M., Heydari, R., Pourjam, E. & Atighi, M.R. (2014) Description of Ektaphelenchoides fuchsi n. sp. (Nematoda: Ektaphelenchinae) from western Iran. Zootaxa, 3846 (3), 430–438.
    http://dx.doi.org/10.11646/zootaxa.3846.3.6

    Fuchs, A.G. (1930) Neue an Borken- und Rüsselkäfer gebundene Nematoden, halbparasitische und Wohnungseinmieter. Zoologische Jahrbücher, Abteilung für Systematik, Ökologie und Geographie der Tiere, 59, 505–646.

    Fuchs, A.G. (1937) Neue parasitische un halbparasitische Nematoden bei Borkenkäfern und einige andere Nematoden. I. Teil. Zoologische Jahrbücher, Abteilung für Systematik, Ökologie und Geographie der Tiere, 70, 291–380.

    Hall, T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41, 95–98.

    Hechler, H.C. & Taylor, D.P. (1965) Taxonomy of the genus Seinura (Nematoda: Aphelenchoididae), with descriptions of S. celeries n. sp. and S. steineri n. sp. Proceedings of the Helminthological Society of Washington, 32, 205–219.

    Heydari, R., Tanha Maafi, Z. & Decraemer, W. (2014) Morphological and molecular characterisation of Trichodorus golestanensis (Diphtheorphorina, Trichodoridae), a new species from Iran. European Journal of Plant Pathology, 140, 329–340.
    http://dx.doi.org/10.1007/s10658-014-0466-x

    Hunt, D.J. (1993) Aphelenchida, Longidoridae and Trichodoridae: Their systematics and bionomics. CABI Publishing, Wallingford, 352 pp.

    Hunt, D.J. (2008) A checklist of the Aphelenchoidea (Nematoda: Tylenchina). Journal of Nematode Morphology and Systematics, 10, 99–135.

    Kakulia, G.A. (1967) [New nematode genus, Devibursaphelenchus n. g. (Nematoda: Aphelenchoididae).] Soobschcheniya Akademii Nauk Gruzinskoi SSR, 47, 439–443. [in Russian]

    Kanzaki, N., Maehara, K., Aikawa, T., Masuya, H. & Giblin-Davis, R.M. (2011) Description of Bursaphelenchus kiyoharai n. sp. (Tylenchina: Aphelenchoididae) with remarks on the taxonomic framework of the Parasitaphelenchinae Rühm, 1956 and Aphelenchoidinae Fuchs, 1937. Nematology, 13, 787–804.

    Katoh, K. & Standley, D.M. (2013) MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Molecular Biology and Evolutions, 30, 772–780.
    http://dx.doi.org/10.1093/molbev/mst010

    Korenchenko, E.A. (1987) [Cryptaphelenchus diversispicularis n. sp. (Tylenchida, Aphelenchoididae) – a new nematode of the bark beetle, Ips subelongatus (Coleoptera: Ipidae).] Parazitologiya, 21, 73–78. [in Russian]

    Kurashvili, B.E., Kakulia, G.A. & Devdariani, Ts. G. (1980) [Parasitic nematodes of the bark-beetles in Georgia.] Metsniereba, Tbilisi, 172 pp. [in Russian]

    Larget, B. & Simon, D.L. (1999) Markov chain Monte Carlo algorithms for the Bayesian analysis of phylogenetic trees. Molecular Biology and Evolution, 16, 750–759.
    http://dx.doi.org/10.1093/oxfordjournals.molbev.a026160

    Massey, C.L. (1974) Biology and taxonomy of nematode parasites and associates of bark beetles in the United States. Agriculture Handbook No. 446. USDA, Forest Service, Washington, v + 233 pp.

    Nunn, G.B. (1992) Nematode Molecular Evolution. Ph.D. dissertation, University of Nottingham, 47 pp.

    Remillet, M. & Silvain, J.F. (1988) Noctuidonema guyanense n. g., n. sp. (Nematoda: Aphelenchoididae) ectoparasite of noctuids of the genus Spodoptera (Lepidoptera: Noctuidae). Revue de Nématologie, 11, 21–24.

    Pedram, M., Pourjam, E., Atighi, M.R., Ye, W. & Houshmand, A. (2012) Ektaphelenchoides sylvestris sp. nov. (Nematoda: Ektaphelenchinae) from Iran. Annales Zoologici, 62, 325–329.
    http://dx.doi.org/10.3161/000345412X652864

    Ronquist, F. & Huelsenbeck, J.P. (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics, 19, 1572–1574.
    http://dx.doi.org/10.1093/bioinformatics/btg180

    Rühm, W. (1956) Die Nematoden der Ipiden. Parasitologische SchriftenReihe, 6, 1–435.

    Silvestro, D. & Michalak, I. (2012) RaxmlGUI: a graphical front-end for raxML. Organisms Diversity & Evolution, 12, 335–337.

    Skarbilovich, T.S. (1947) [Revision of the systematics of the nematode family Anguillulinidae Baylis and Daubney, 1926.] Doklady Akademii Nauk SSR, 57, 307–308. [in Russian]

    Stöver, B.C. & Müller, K.F. (2010) TreeGraph 2: combining and visualizing evidence from different phylogenetic analyses. BMC Bioinformatics, 11, 7.

    Yaghoubi, A., Pourjam, E., Atighi, M.R. & Pedram, M. (2014) Molecular and morphological characterization of Ektaphelenchoides ruehmi sp. n. (Nematoda: Ektaphelenchinae) from southwestern Iran. Russian Journal of Nematology, 20 (1), 37–44.

    Zhao, Z.Q., Ye, W.M., Giblin-Davis, R.M., Li, D.M., Thomas, W.K., Davies, K.A. & Riley, I.T. (2008) Morphological and molecular analysis of six aphelenchoidoids from Australian conifers and their relationship to Bursaphelenchus (Fuchs, 1937). Nematology, 10, 663–678.
    http://dx.doi.org/10.1163/156854108785787299