Abstract
A new species of the genus Amaurobius C.L. Koch, 1837, Amaurobius pesarinii sp. n., from the Apennine Mountains (Italy) is described on the basis of both sexes. The male of A. pavesii Pesarini, 1991, previously unknown, is also described. Females of A. pavesii and both sexes of the similar A. scopolii Thorell, 1871 are re-described. Detailed description of morphological characters allow clear separation between these species and the closely related A. ferox (Walckenaer, 1820). Their phylogenetic relationships among the most common Italian Amaurobius species are discussed on the basis of molecular data (partial fragments of the genes COI, 16S and H3), together with their distribution across the Italian Peninsula. This is the first contribution to a multi-locus phylogenetic tree of European Amaurobius species. The synonymy of A. sciakyi Pesarini, 1991 with A. ruffoi Thaler, 1990 is proposed based on morphological characters.
References
Arnedo, M.A., Oromí, P. & Ribera, C. (2001) Radiation of the spider genus Dysdera (Araneae, Dysderidae) in the Canary Islands: cladistic assessment based on multiple data sets. Cladistics, 17, 313–353.
https://doi.org/10.1111/j.1096-0031.2001.tb00129.xBacelar, A. (1927) Aracnidios Portugueses II. Bulletin da Sociedade Portuguesa de Sciencias Naturais, 10, 129–138.
Ballarin, F., Pantini, P. & Hansen, H. (2011) Catalogo ragionato dei ragni (Arachnida, Araneae) del Veneto. Memorie del Museo Civico di Storia Naturale di Verona 2aserie. Sezione Scienze della Vita, 21, 1–151.
Barrett, R.D.H. & Hebert, P.D.N. (2005) Identifying spiders through DNA barcodes. Canadian Journal of Zoology, 83, 481–491.
https://doi.org/10.1139/z05-024Barrientos, J.A. & Ferrandez, M.A. (1982) La coleccion de Araneidos del Departamento de Zoologia de la Universidad de Salamanca, III: aranas migalomorfas, haploginas y cribeladas. Boletin de la Asociacion Espanola de Entomologia, 5, 75–86.
Brignoli, P.M. (1972) Catalogo dei ragni cavernicoli italiani. Quaderni di speleologia. Circolo Speleologico Romano, 1, 5–211.
Caoduro, G., Osella, G. & Ruffo, S. (1994) La fauna cavernicola della regione veronese. Memorie del Museo Civico di Storia Naturale di Verona 2aserie. Sezione Scienze della Vita, 11, 1–144.
Colgan, D., McLauchlan, A., Wilson, G., Livingston, S., Edgecombe, G., Macaranas, J., Cassis, G. & Gray, M. (1998) Histone H3 and U2 snRNA DNA sequences and arthropod molecular evolution. Australian Journal of Zoology, 46, 419–437.
https://doi.org/10.1071/ZO98048Caporiacco, L. di (1930) Un nuovo opilone cavernicolo. Monitore Zoologico Italiano, 41 (5), 125–127.
Caporiacco, L. di (1940) Arachniden aus der Province Verona (Norditalien). Folia Zoologica et Hydrobiologica, 10 (1), 1–37.
Dresco, E. (1949) Note sur les araignées de quelques grottes de l’Italie Méridionale et description d’une espèce nouvelle. Studi Speleologici e Faunistici sull’Italia Meridionale, supplemento al Bollettino della Società dei Naturalisti in Napoli, 12, 1–6.
Dresco, E. & Hubert, M. (1969) Araneae speluncarum Italiae I. Fragmenta Entomologica, 6 (2), 167–181.
Folmer, O., Black, M., Hoeh, W., Lutz, R. & Vrijenhoek, R. (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3, 294–299.
GenBank (2016) Available from: https://www.ncbi.nlm.nih.gov/genbank/ (accessed 20 March 2016)
Hall, T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Sympposium Series, 41, 95–98.
Hedin, M.C. & Maddison, W.P. (2001) A combined molecular approach to phylogeny of the jumping spider subfamily Dendryphantinae (Araneae: Salticidae). Molecular Phylogenetics and Evolution, 18, 386–403.
https://doi.org/10.1006/mpev.2000.0883Hubert, M. (1965) Remarques sur quelques espèces d'araignées du genre Amaurobius C. Koch, 1837 et description d'une espèce nouvelle. Bulletin du Muséum National d’Histoire Naturelle, 2e Série, 36, 784–796.
Isaia, M., Pantini, P., Beikes, S. & Badino, G. (2007) Catalogo ragionato dei ragni (Arachnida, Araneae) del Piemonte e della Lombardia. Memorie dell’Associazione Naturalistica Piemontese, 9, 1–162.
Katoh, K. & Standley, D.M. (2013) MAFFT Multiple Sequence Alignment Software Version 7: Improvements in Performance and Usability. Molecular Biology and Evolution, 30, 772–780.
https://doi.org/10.1093/molbev/mst010Kratochvíl, J. (1935) Araignees nouvelles ou non encore signalees en Yougoslavie. Folia Zoologica, 3, 10–25.
Maddison, W.P. & Maddison D.R. (2015) Mesquite: a molecular system for evolutionary analysis. Version 3.04. Available from: http://mesquiteproject.org (accessed 15 May 2017)
Miller, J.A., Carmichael, A., Ramirez, M.J., Spagna, J.C., Haddad, C.R., Rezac, M., Johannesen, J., Kral, J., Wang, X.P. & Griswold, C.E. (2010) Phylogeny of entelegyne spiders: affinities of the family Penestomidae (NEW RANK), generic phylogeny of Eresidae, and asymmetric rates of change in spinning organ evolution (Araneae, Araneoidea, Entelegynae). Molecular Phylogenetics and Evolution, 55, 786–804.
https://doi.org/10.1016/j.ympev.2010.02.021Nentwig, W., Blick, T., Gloor, D., Hänggi, A. & Kropf, C. (2016) Araneae. Spiders of Europe. Version 01.2016. Available from: http://www.araneae.unibe.ch/ (accessed 20 March 2016)
Nikolic, F. & Polenec, A. (1981) Aranea. Catalogus Faunae Jugoslaviae, 3 (4), 1–135.
Pantini, P. & Isaia, M. (2016) Checklist of the Italian Spiders. Version April 2016. Available from: http://www.museoscienzebergamo.it/web/index.php?option=com_content&view=category&layout=blog&id=96&Itemid=94 (accessed 20 April 2016)
Peru, B. le (2007) Catalogue et répartition des araignées de France. Revue Arachnologique, 16, 1–468.
Pesarini, C. (1991) The Amaurobiidae of northern Italy. Atti della Società Italiana di Scienze Naturali e del Museo Civico di Storia Naturale di Milano, 131, 261–276.
Simon, E. (1914) Les Arachnides de France. 6. 1è partie, Le Synopsis général et le catalogue des espèces françaises de l'ordre des Araneae. Roret, Paris, 308 pp.
Simon, C., Frati, F., Beckenbach, A., Crespi, B., Liu, H. & Flook, P. (1994) Evolution, weighting, and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers. Annals of the entomological Society of America, 87, 651–701.
https://doi.org/10.1093/aesa/87.6.651Stamatakis, A. (2014) RAxML Version 8: A tool for Phylogenetic Analysis and Post-Analysis of Large Phylogenies. Bioinformatics, 30, 1312–1313.
https://doi.org/10.1093/bioinformatics/btu033Strand, E. (1916) XX. Spinnen und Opilionen aus Griechenland, Albanien und Kleinasien. Archiv für Naturgeschichte, Abteilung A., 8 (2), 158–167.
Tamura, K., Stecher, G., Peterson, D., Filipski, A. & Kumar, S. (2013) MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0. Molecular Biology and Evolution, 30, 2725–2729.
https://doi.org/10.1093/molbev/mst197Thaler, K. (1990). Amaurobius ruffoi n.sp., eine weitere Reliktart der Südalpen—mit Bemerkungen über die Amaurobiidae der Alpen (Arachnida: Aranei). Zoologischer Anzeiger, 225, 241–252.
Thaler, K. & Knoflach, B. (1998) Phyxelida anatolica Griswold, new to Cyprus (Arachnida Araneae: Amaurobiidae, Phyxelidinae). Bulletin of the British Arachnological Society, 11, 36–40.
Thorell, T. (1871) Remarks on synonyms of European spiders. Part II. C.J. Lundström, Uppsala, 138 pp. [pp. 97–228]
Trotta, A. (2005) Introduzione ai Ragni italiani (Arachnida Araneae). Memorie della Società Entomologica Italiana, 83, 3–178.
https://doi.org/10.4081/memorieSEI.2004.3World Spider Catalog (2016) World Spider Catalog. Natural History Museum Bern. Version 17.0. Available from: http://wsc.nmbe.ch (accessed 20 March 2016)