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Issue: Vol. 4299 No. 2: 27 Jul. 2017
Type: Article
Published: 2017-07-27
DOI: 10.11646/zootaxa.4299.2.6
Page range: 263–270
Abstract views: 72
PDF downloaded: 1

Life-stage association of black flies, using a fast-evolving nuclear gene sequence, and description of the larva of Simulium lampangense Takaoka & Choochote (Diptera: Simuliidae) from Thailand

Department of Biology, Faculty of Science, Mahasarakham University, Kantharawichai District, Maha Sarakham 44150, Thailand.
Department of Biology, Faculty of Science, Mahasarakham University, Kantharawichai District, Maha Sarakham 44150, Thailand.
Department of Plant & Environmental Sciences, Clemson University, Clemson, SC 29634-0310 USA
Diptera DNA barcode ECP1 sequence phylogeny Simulium multistriatum species group Thailand

Abstract

Larval black flies, especially earlier instars, can be difficult to associate morphologically and chromosomally with other life stages. We used sequences of mitochondrial cytochrome c oxidase I (COI) and nuclear elongation complex protein 1 (ECP1) to associate unknown larvae with known species of the Simulium multistriatum species group in Thailand. COI barcode sequences failed to differentiate closely related species (S. chaliowae, S. lampangense, and S. fenestratum) and unknown larvae. In contrast, ECP1 sequences clearly separated these species, and revealed that the unknown larvae clustered with pupae of S. lampangense. The larva of S. lampangense is described morphologically for the first time. It is similar to that of S. chaliowae, but can be distinguished by a pair of dorsal protuberances on segments 3–7 rather than on segments 2–6.

 

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