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Type: Article
Published: 2017-10-30
Page range: 124–134
Abstract views: 129
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Taxonomy of the zodiac loaches, Mesonoemacheilus triangularis, M. tambaraparniensis and M. periyarensis, endemic to the Western Ghats of India (Teleostei: Nemacheilidae)

School of Ocean Science and Technology, Kerala University of Fisheries and Ocean Studies (KUFOS), Kochi, India
Indian Institute of Science Education and Research (IISER), Pune, India
Department of Fisheries Resource Management, Kerala University of Fisheries and Ocean Studies (KUFOS), Kochi, India Center for Excellence in Aquatic Resource Management and Conservation, Kerala University of Fisheries and Ocean Studies (KUFOS), Kochi, India
Department of Fisheries Resource Management, Kerala University of Fisheries and Ocean Studies (KUFOS), Kochi, India Center for Excellence in Aquatic Resource Management and Conservation, Kerala University of Fisheries and Ocean Studies (KUFOS), Kochi, India
Pisces freshwater fish India nemacheilidae taxonomy Western Ghats


The Nemacheilid loaches, Noemacheilus triangularis tambaraparniensis and Nemacheilus periyarensis have been widely considered to be synonyms of the zodiac loach, Mesonoemacheilus triangularis. An integrative taxonomic investigation based on topotypic material, involving molecular analysis of two mitochondrial genes (cytochrome oxidase subunit 1 and cytochrome b), multivariate morphometric analysis based on non-metric multidimensional scaling and non-parametric multivariate analysis of variance suggests that the three species are in fact distinct.



  1. Ali, A., Philip, S., Dahanukar, N., Renjithkumar, C.R., Bijukumar, A. & Raghavan, R. (2013) Distribution, threats and conservation status of Hypselobarbus thomassi (Day, 1874), a poorly known cyprinid fish of the Western Ghats freshwater ecoregion. Journal of Threatened Taxa, 5, 5202–5213.

    Anderson, M.J. (2001) A new method for non parametric multivariate analysis of variance. Austral Ecology, 26 (1), 32–46.

    Arunachalam, M. & Sankaranarayanan, A. (1999) Fishes of Gadana River in Kalakad Mundanthurai Tiger Reserve. Journal of Bombay Natural History Society, 96 (2), 232–238.

    Clarke, K.R. (1993) Non-parametric multivariate analyses of changes in community structure. Australian Journal of Ecology, 18, 117–143.

    Dahanukar, N. & Raghavan, R. (2013) Freshwater fishes of the Western Ghats, Checklist V 1.0, August 2013. MIN, Newsletter of IUCN SSC/WI Freshwater Fish Specialist Group - South Asia and the Freshwater Fish Conservation Network of South Asia (FFCNSA), 1, 6–16.

    Day, F. (1865a) On the fishes of Cochin, on the Malabar coast of India. Part II, Anacanthini. Proceedings of the Zoological Society of London, 33, 286–318.

    Day, F. (1865b) The fishes of Malabar. Bernard Quaritch, London, 293 pp.

    Day, F. (1876–1878) The fishes of India; being a natural history of the fishes known to Inhabit the seas and fresh waters of India, Burma, and Ceylon. Bernard Quaritch, London, 816 pp.

    Day, F. (1889) Fishes. In: Blanford, W.T. (Ed.), The Fauna of British India, including Ceylon and Burma. Taylor & Francis, London, 479 pp.

    Devi, K.R. & Indra, T.J. (2002) A note on Mesonoemacheilus herrei, Nalbant and Banarescu (Cypriniformes: Balitoridae: Noemacheilinae). Journal of the Bombay Natural History Society, 99 (2), 333–337.

    Edgar, R.C. (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research, 32 (5), 1792–1797.

    Hammer, Ø., Harper, D.A.T. & Ryan, P.D. (2001) PAST: paleontological statistics software package for education and data analysis. Palaeontologia Electronica, 4, 1–9.

    Hora, S.L. & Law, N.C. (1941) The freshwater fishes of Travancore. Records of the Indian Museum, 43, 233–256.

    Jayaram, K.C. (2010) Freshwater Fishes of the Indian Region. 2nd Edition. Narendra Publishing House, New Delhi, 616 pp.

    Johnson, J.A. & Arunachalam, M. (2009) Diversity, distribution and assemblage structure of fishes in streams of southern Western Ghats, India. Journal of Threatened Taxa, 1 (10), 507–513.

    Katwate, U., Paingankar, M.S., Jadhav, S. & Dahanukar, N. (2013) Phylogenetic position and osteology of Pethia setnai (Chhapgar & Sane, 1992), an endemic barb (Teleostei: Cyprinidae) of the Western Ghats, India, with notes on its distribution and threats. Journal of Threatened Taxa, 5, 5214–5227.

    Keskar, A., Kumkar, P., Katwate, U., Ali, A., Raghavan, R. & Dahanukar, N. (2015) Redescription of Nemachilichthys rueppelli, a senior synonym of N. shimogensis (Teleostei: Nemacheilidae). Zootaxa, 4059 (3), 555–568.

    Kottelat, M. (2012) Conspectus Cobitidium: an inventory of the loaches of the world (Teleostei: Cypriniformes: Cobitoidea). Raffles Bulletin of Zoology, 26 (Supplement), pp 1–199.

    Kumar, S., Stecher, G. & Tamura, K. (2016) MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Molecular Biology and Evolution, 33, 1870–1874.

    Kurup, B.M. & Radhakrishnan, K.V. (2005) Fishes of the genus Nemacheilus (Bleeker 1863) in Kerala with a description of a new species, Nemacheilus periyarensis. Journal of the Bombay Natural History Society, 102 (1), 7578.

    Lanfear, R., Calcott, B., Ho, S.Y. & Guindon, S. (2012) PartitionFinder: combined selection of partitioning schemes and substitution models for phylogenetic analyses. Molecular Biology and Evolution, 29 (6), 1695–1701.

    Menon, A.G.K. (1987) The fauna of India and the adjacent countries. Pisces. Vol. IV, Teleostei – Cobitoidea. Part 1. Homalopteridae. Zoological Survey of India, New Delhi, 259 pp.

    Minh, B.Q., Nguyen, M.A.T. & von Haeseler, A. (2013) Ultrafast approximation for phylogenetic bootstrap. Molecular Biology and Evolution, 30, 1188–1195.

    Nei, M. & Kumar, S. (2000) Molecular Evolution and Phylogenetics. Oxford University Press, Oxford, 333 pp.

    Nguyen, L.T., Schmidt, H.A., von Haeseler, A. & Minh, B.Q. (2015) IQ-TREE: A fast and effective stochastic algorithm for estimating maximum likelihood phylogenies. Molecular Biology and Evolution, 32, 268–274.

    Rambaut, A. (2009) FigTree. v1. 3.1. Available from: (accessed 2 November 2016)

    Schwarz, G. (1978) Estimating the dimension of a model. Annals of Statistics, 6, 461–464.

    Singh, A., Sen, N., Bănărescu, P. & Nalbant, T.T. (1982) New noemacheiline loaches from India (Pisces: Cobitidae). Travaux du Muséum d’Histoire Naturelle “Grigore Antipa”, 23, 201–212.

    Talwar, P.K. & Jhingran, A.G. (1991) Inland fishes of India and adjacent countries. Vols. 1 & 2. Oxford and IBH Publishing Company, New Delhi, Bombay & Calcutta, 1158 pp.

    Tamura, K. & Nei, M. (1993) Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Molecular Biology and Evolution, 10 (3), 512–526.