Skip to main content Skip to main navigation menu Skip to site footer
Type: Article
Published: 2017-11-17
Page range: 251–283
Abstract views: 133
PDF downloaded: 2

Morphological and molecular analyses of epikarstic Parastenocarididae (Copepoda: Harpacticoida) from two Sicilian caves, with description of a new Stammericaris

Department of Sustainable Ecosystems and Bioresources, Research and Innovation Centre, Fondazione Edmund Mach, Via E. Mach 1, 38010 S. Michele all’Adige (TN), Italy
Department for Innovation in Biological, Agro-food and Forest systems, Tuscia University, Largo dell’Università snc, 01100 Viterbo, Italy
Department of Biodiversity and Molecular Ecology, Research and Innovation Centre, Fondazione Edmund Mach, Via E. Mach 1, 38010 S. Michele all’Adige (TN), Italy
Department of Biodiversity and Molecular Ecology, Research and Innovation Centre, Fondazione Edmund Mach, Via E. Mach 1, 38010 S. Michele all’Adige (TN), Italy
Department of Sustainable Ecosystems and Bioresources, Research and Innovation Centre, Fondazione Edmund Mach, Via E. Mach 1, 38010 S. Michele all’Adige (TN), Italy
Department of Biological, Geological and Environmental Sciences, Catania University, Via Androne 81, 95124 Catania, Italy
Department of Biological, Geological and Environmental Sciences, Catania University, Via Androne 81, 95124 Catania, Italy
Crustacea 18S rDNA gene COI gene crustacean evaporitic cave stygofauna

Abstract

We describe Stammericaris destillans sp. nov., and re-describe Stammericaris trinacriae (Pesce, Galassi and Cottarelli 1988) based on new material. The two species were collected from epikarstic drips and pools on the floor of two different caves: a karstic (Molara Cave) and a gypsum (Entella Cave) cave, respectively, both located in Sicily, Italy. We also report the presence of previously undescribed structures for Stammericaris amyclaea (Cottarelli 1969) and Stammericaris orcina (Chappuis 1938). Phylogenetic analyses of the mitochondrial COI and ribosomal 18S genes attributed the new species to Stammericaris Jakobi 1972, therefore the structure of the male P4 endopod of S. destillans is interpreted as an autapomorphy; other morphological features (structure of male antennule and P3, of female P3; inner ornamentation of P1 basis, armature of caudal rami and shape and armature of P5 of both sexes) correspond to those typical of the genus. Hence, we slithgly amended the generic diagnosis. [zoobank.org:pub:4CC84A0C-C511-4388-9728-41647E58097A]

 

References

  1. Abbate, R. (2011) Conoscenza, salvaguardia e utilizzo didattico del geosito carsico Montagnola di Santa Rosalia (Palermo). Atti del XXI Congresso Nazionale di Speleologia – Sessione Didattica, Trieste 2–5 Giugno 2011, 125133.

    Blanco-Bercial, L., Cornils, A., Copley, N., Bucklin, A. (2014) DNA barcoding of marine Copepods: assessment of analytical approaches to species identification. PLOS Currents, 6.
    https://doi.org/10.1371/currents.tol.cdf8b74881f87e3b01d56b43791626d2

    Brancelj, A. (2002) Microdistribution and high diversity of copepoda (Crustacea) in a small cave in central Slovenia. Hydrobiologia, 477, 59–72.
    https://doi.org/10.1023/A:1021043014879

    Brancelj, A. (2003) Biological sampling for epikarst water. In: Jones WK, Culver DC, Herman JS, eds. Epikarst. Proceedings of the Karst Water Institute Symposium; 14 Octobre 2003, Sheperdstown (WV). Karts Water Institute Special Publication, 9, 99–103.

    Brancelj, A. (2009) Fauna of an unsaturated karstic zone in Central Slovenia: two new species of Harpacticoida (Crustacea: Copepoda), Elaphoidella millennii n. sp. and E. tarmani n. sp., their ecology and morphological adaptations. Hydrobiologia, 621, 85–104. https://doi.org/10.1007/s10750-008-9634-3

    Bruno, M.C. & Cottarelli, V. (2015) First record of Kinnecaris (Copepoda: Harpacticoida: Parastenocarididae) from Turkey and from Thailand; description of three new species and redefinition of the genus. Italian Journal of Zoology, 82, 1–26.
    https://doi.org/10.1651/09-3201.1

    Catalano, R. (1986) Le evaporiti messiniane. Loro ruolo nell’evoluzione geologica della Sicilia: Le Grotte d’Italia, 13, 109–122.

    Corgosinho, P.H.C., Martinéz Arbizu, P.M. & Dos Santos Silva, E.N. (2007) Redescription of Remaneicaris ignotus (Dussart, 1983), a Parastenocarididae (Copepoda, Harpacticoida) with an unusual set of plesiomorphic characters. Invertebrate Zoology, 4, 31–44.

    Corgosinho, P.H.C., Mercado-Salas, N.F., Martinéz Arbizu, P.M., Dos Santos Silva, E.N. & Kihara, T.C. (2017) Revision of the Remaneicaris argentina–group (Copepoda, Harpacticoida, Parastenocarididae): supplementary description of species, and description of the first semiterrestrial Remaneicaris from the tropical forest of Southeast Mexico. Zootaxa, 4238 (4), 499–530.
    https://doi.org/10.11646/zootaxa.4238.4.2

    Cottarelli, V. (1969) Nuove Parastenocaris (Copepoda, Harpacticoida) dell'Italia centromeridionale. Rivista di Idrobiologia, 8, 128.

    Cottarelli, V., Bruno, M.C., Spena, M.T. & Grasso, R. (2012) Studies on subterranean copepods from Italy, with descriptions of two new epikarstic species from a cave in Sicily. Zoological Studies, 51, 556–82.

    Cottarelli, V. & Drigo, E. (1972) Sulla presenza di Parastenocaris orcina Chappuis (Cop. Harpacticoida) in acque interstiziali del Lago di Bracciano. Notiziario del Circolo Speleologico Romano, 17, 51–54.

    Cottarelli, V., Bruno, M.C. & Venanzetti, F. (1994) Ricerche zoologiche della nave oceanografica "Minerva" (C.N.R.) sulle isole circumsarde. XVIII. First record in phreatic freshwater of harpacticoids belonging to the genus Arenopontia (Crustacea, Copepoda) and description of two new species. Annali del Museo Civico di Storia Naturale "G. Doria", 15, 471–484.

    Darriba, D., Taboada, G.L., Doallo, R. & Posada, D. (2012) jModelTest 2: more models, new heuristics and parallel computing. Nature Methods, 9, 772.
    https://doi.org/10.1038/nmeth.2109

    Delamare Deboutteville, C. (1960) Biologie des eaux souterraines littorales et continentals. Hermann, Paris, 740 pp.

    Di Maggio, C., Madonia, G., Parise, M. & Vattano, M. (2012) Karst of Sicily and its conservation. Journal of Cave and Karst Studies, 74, 157–172.
    https://doi.org/10.4311/2011JCKS0209

    Folmer, O., Black, M., Hoeh, W., Lutz, R. & Vrijenhoek, R. (1994) DNA primers for amplification of mithocondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3, 294–299.

    Galassi, D.M.P. (1997) Little known harpacticoid copepods from Italy, and description of Parastenocaris crenobia n. sp. (Copepoda, Harpacticoida). Crustaceana, 70, 694–709.
    https://doi.org/10.1163/156854097X00131

    Galassi, D.M.P. & De Laurentiis, P. (2004) Towards a revision of the genus Parastenocaris Kessler, 1913: establishment of Simplicaris gen. nov. from groundwaters in central Italy and review of the P. brevipes–group (Copepoda, Harpacticoida, Parastenocarididae). Zoological Journal of the Linnean Society, 140, 417–436.
    https://doi.org/10.1111/j.1096-3642.2003.00107.x

    Galassi, D.M.P., Stoch, F., Fiasca, B., Di Lorenzo, T. & Gattone, E. (2009) Groundwater biodiversity patterns in the Lessinian Massif of northern Italy. Freshwater Biology, 54, 830–847.
    https://doi.org/10.1111/j.1365-2427.2009.02203.x

    Grasso, R., Spena, M.T., Di Franco, D., Catalano, A., Messana, V., Polito, C., Di Maggio, G. & Cottarelli, V. (2015) Epikarst e vaschette di stillicidio: contributo alla conoscenza della meiofauna stigobia della Grotta di Entella (PA). In: Madonia, G., Panzica La Manna, M., Vattano, M. (Eds), Atti del 5 congresso regionale di speleologia della Sicilia, Santa Ninfa (TP), 23/24 Novembre 2013. Federazione Speleologica Regionale Siciliana, Palermo. [in press]

    Guindon, S., Dufayard, J.F., Lefort, V., Anisimova, M., Hordijk, W. & Gascuel, O. (2010) New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. Systematic Biology, 59, 307–321.
    https://doi.org/10.1093/sysbio/syq010

    Huelsenbeck, J.P. & Ronquist, F. (2001) MRBAYES: Bayesian inference of phylogeny. Bioinformatics, 17, 754–755.
    https://doi.org/10.1093/bioinformatics/17.8.754

    Huys, R. & Boxshall, G.A. (1991) Copepod Evolution. The Ray Society, London, 468 pp.

    Jakobi, H. (1972) Trends (Enp. P4) innerhalb der Parastenocarididen (Copepoda Harpacticoidea). Crustaceana, 22, 127–146.
    https://doi.org/10.1163/156854072X00390

    Karanovic, T. (2006) Subterranean copepods (Crustacea, Copepoda) from the Pilbara region in Western Australia. Records of the Western Australian Museum, Supplement, 70, 1–239. https://doi.org/10.18195/issn.0313-122x.70.2006.001-239

    Karanovic, T., Cho, J.-L. & Lee, W. (2012) Redefinition of the parastenocaridid genus Proserpinicaris (Copepoda: Harpacticoida), with description of three new species from Korea. Journal of Natural History, 46, 1573–1613.
    https://doi.org/10.1080/00222933.2012.681316

    Karanovic, T. & Cooper, S.J.B. (2011a) Molecular and morphological evidence for short range endemism in the Kinnecaris solitaria complex (Copepoda: Parastenocarididae), with descriptions of seven new species. Zootaxa, 3026, 1–64.

    Karanovic, T. & Cooper, S.J.B. (2011b) Third genus of parastenocaridid copepods from Australia supported by molecular evidence (Copepoda, Harpacticoida). In: Defaye, D., Suarez-Morales, E. & Vaupel Klein, J.C. (Eds.), Studies on Freshwater Copepoda: a Volume in Honour of Bernard Dussart. Brill, Leiden/Bostonpp, pp. 293–337.
    https://doi.org/10.1163/9789004188280_014

    Karanovic, T., Kim, K. & Grygier, M.J. (2015) A new species of Schizopera (Copepoda: Harpacticoida) from Japan, its phylogeny based on the mtCOI gene and comments on the genus Schizoperopsis. Journal of Natural History, 49, 1–32. https://doi.org/10.1080/00222933.2015.1028112

    Karanovic, T., Kim, K. & Lee, W. (2014) Morphological and molecular affinities of two East Asian species of Stenhelia (Crustacea, Copepoda, Harpacticoida). ZooKeys, 411, 105–143. https://doi.org/10.3897/zookeys.411.7346

    Kumar, S. & Gadagkar, S.R. (2001) Disparity Index: A simple statistic to measure and test the homogeneity of substitution patterns between molecular sequences. Genetics, 158, 1321–1327.

    Lefébure, T., Douady, C.J., Gouy, M. & Gibert, J. (2006) Relationship between morphological taxonomy and molecular divergence within Crustacea: proposal of a molecular threshold to help species delimitation. Molecular Phylogenetics and Evolution, 40, 435–447. https://doi.org/10.1016/j.ympev.2006.03.014

    Lopez, J.V., Yuhki, N., Masuda, R., Modi, W. & O’Brien, S.J. (1994) Numt, a recent transfer and tandem amplification of mitochondrial DNA to the nuclear genome of the domestic cat. Journal of Molecular Evolution, 39, 174–190.

    Mazzini, I., Marrone, F., Arculeo, M. & Rossetti, G. (2017) Revision of recent and fossil Mixtacandona Klie 1938 (Ostracoda, Candonidae) from Italy, with description of a new species. Zootaxa, 4221 (3), 323–340.
    https://doi.org/10.11646/zootaxa.4221.3.3

    Miller, M.A., Pfeiffer, W. & Schwartz, T. (2010) Creating the CIPRES Science Gateway for inference of large phylogenetic trees. In: Institute of Electrical and Electronics Engineers (Eds.), Proceedings of the Gateway Computing Environments Workshop (GCE), 14 Nov. 2010. New Orleans, LA, pp. 1–8.
    https://doi.org/10.1109/GCE.2010.5676129

    Pesce, G.L. (1988) A new species of Schizopera Sars 1905 from groundwaters of Sicily, Italy (Crustacea Copepoda: Diosaccidae). Senckenbergiana Biologica, 68, 413–417.

    Pesce, G.L. & Galassi, D.M.P. (1987) Copepodi di acque sotterranee della Sicilia. Animalia, 14, 193–235.

    Pesce, G.L. & Galassi, D.M.P. (1988) Una nuova Attheyella di acque sotterranee italiane: Attheyella (Brehmiella) paranaphtalica n. sp. (Crustacea Copepoda: Canthocamptidae). Fragmenta Entomologica, 21, 1–7.

    Pesce, G.L., Galassi, D.M.P. & Apostolov, A. (1987) The genus Elaphoidella Chappuis (Copepoda: Harpacticoida) in Italy, including the description of five new species. Bollettino di Zoologia, 54, 177–185.
    https://doi.org/10.1080/11250008709355579

    Pesce, G.L., Galassi, D.M.P. & Cottarelli, V. (1988) First representative of the family Parastenocaridae from Sicily (Italy), and description of two new species of Parastenocaris Kessler (Crustacea Copepoda: Harpacticoida). Bulletin Zoölogisch Museum, Universiteit van Amsterdam, 11, 137–141.

    Pipan, T. & Brancelj, A. (2004) Distribution patterns of copepods (Crustacea: Copepoda) in percolation water of the Postojnska Jama Cave System (Slovenia). Zoological Studies, 43, 206–210.

    Pipan, T. & Culver, D.C. (2006) Copepod distribution as an indicator of epikarst system connectivity. Hydrogeology Journal, 15, 817–822.
    https://doi.org/10.1007/s10040-006-0114-4

    Pipan, T., Holt, N. & Culver, D.C. (2010) How to protect a diverse, poorly known, inaccessible fauna: identification and protection of source and sink habitats in the epikarst. Aquatic Conservation: Marine & Freshwater Ecosystems, 20, 748–755.
    https://doi.org/10.1002/aqc.1148

    Rambaut, A. (2009) Tree figure drawing tool V 1.3.1. Available from: http://tree.bio.ed.ac.uk/ (15 July 2014)

    Schminke, H.K. (2010) High-level phylogenetic relationships within Parastenocarididae (Copepoda, Harpacticoida). Crustaceana, 83, 343–367.
    https://doi.org/10.1163/001121610X12627655658168

    Schminke, H.K. (2013) Stammericaris Jakobi, 1972 redefined and a new genus of Parastenocarididae (Copepoda, Harpacticoida). Crustaceana, 86, 704–717.
    https://doi.org/10.1163/15685403-00003196

    Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M. & Kumar, S. (2011) MEGA5: Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Molecular Biology and Evolution, 28, 2731–2739.
    https://doi.org/10.1093/molbev/msr121

    Tang, C.Q., Leasi, F., Obertegger, U., Kieneke, A., Barraclough, T.G. & Fontaneto, D. (2012) The widely used small subunit 18S rDNA molecule greatly underestimates true diversity in biodiversity surveys of the meiofauna. Proceedings of the National Academy of Sciences of the United States of America, 109, 16208–16212.
    https://doi.org/10.1073/pnas.1209160109

    Totakura, V.R., Ranga Reddy, Y. & Shaik, S. (2014) Three new species of Parastenocarididae (Crustacea, Copepoda, Harpacticoida) from India. Zootaxa, 3821 (5), 501–537.
    https://doi.org/10.11646/zootaxa.3821.5.1

    Villesen, P. (2007) FaBox: an online toolbox for fast sequences. Molecular Ecology Notes, 7, 965–968.
    https://doi.org/10.3389/fgene.2015.00172

    Zagoskin, M.V., Lazareva, V.I., Grishanin, A.K. & Mukha, D.V. (2014) Phylogenetic Information Content of Copepoda Ribosomal DNA Repeat Units: ITS1 and ITS2 Impact. BioMed Research International, 2014, 926342.
    https://doi.org/10.1155/2014/926342