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Published: 2018-01-08

A new moss salamander, genus Nototriton (Caudata: Plethodontidae), from the Cordillera de Talamanca, in the Costa Rica-Panama border region

Departamento de Zoología, Instituto de Biología, UNAM, AP 70-153 Ciudad Universitaria, CP 04510, México, D.F., México. Escuela de Biología, Universidad de Costa Rica, San Pedro, 11501–2060 San José, Costa Rica.
Costa Rican Amphibian Research Center, Guayacán, Provincia de Limón, Costa Rica.
Amphibia caudate Central America Nototriton picadoi species group Talamanca


A new salamander belonging to the genus Nototriton, subgenus Nototriton, is described from the Caribbean slopes of the southeastern Cordillera de Talamanca in Costa Rica, within Parque Internacional La Amistad, at an elevation ca. 1500 m a.s.l. This new taxon is distinguished from its congeners by its morphological characteristics and by its differentiation in DNA sequences of the 16S rRNA, cytochrome oxidase subunit I (COI), and cytochrome b mitochondrial genes. This new species represents the southernmost extension known for the genus Nototriton.



  1. AmphibiaWeb (2017) AmphibiaWeb: Information on Amphibian Biology and Conservation [web application]. Berkeley, California. Available from: (accessed 13 April 2017)

    Arias, E. (2017) New distribution records for two uncommon amphibian species on the Caribbean slopes of the Cordillera de Talamanca, Costa Rica. Mesoamerican Herpetology, 4, 446–449.

    Bolaños, F. & Wake, D.B. (2009) Two new species of montane web-footed salamanders (Plethodontidae: Bolitoglossa) from the Costa Rica-Panamá border region. Zootaxa, 1981, 57–68.

    Boza-Oviedo, E., Rovito, S.M., Chaves, G., García-Rodríguez, A., Artavia, L.G., Bolaños, F. & Wake, D.B. (2012) Salamanders from the eastern Cordillera de Talamanca, Costa Rica, with descriptions of five new species (Plethodontidae: Bolitoglossa, Nototriton, and Oedipina) and natural history notes from recent expeditions. Zootaxa, 3309, 36–61.

    Clare, E.L., Lim, B.K., Fenton, M.B. & Hebert, P.D.N. (2011) Neotropical bats: estimating species diversity with DNA barcodes. PloS one, 6, e22648.

    Dubois, A. & Raffaëlli, J. (2012) A new ergotaxonomy of the order Urodela Duméril, 1805 (Amphibia, Batrachia). Alytes, 28, 77–161.

    Edgar, R.C. (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research, 32, 1792–1797.

    Fouquet, A., Gilles, A., Vences, M., Marty, C., Blanc, M. & Gemmell, N.J. (2007) Underestimation of species richness in Neotropical frogs revealed by mtDNA analyses. PLoS one, 2, e1109.

    Frost, D.R. (2017) Amphibian Species of the World: an Online Reference. Version 6.0. American Museum of Natural History, New York, USA. Available from: (accessed 23 August 2017)

    García-París, M. & Wake, D.B. (2000) Molecular phylogenetic analysis of relationships of the tropical salamander genera Oedipina and Nototriton, with descriptions of a new genus and three new species. Copeia, 2000, 42–70.[0042:MPAORO]2.0.CO;2

    García-París, M., Parra-Olea, G. & Wake, D.B. (2008) Description of a new species of the Bolitoglossa subpalmata group (Caudata: Plethodontidae) from Costa Rica. Herpetological Journal, 18, 23–31.

    Good, D.A. & Wake, D.B. (1993) Systematic studies of the Costa Rican moss salamanders, genus Nototriton, with descriptions of three new species. Herpetological Monographs, 7, 131–159.

    Holdridge, L.R. (1967) Life Zone Ecology. Tropical Science Center, San José, Costa Rica, 206 pp.

    Johns, G.C. & Avise, J.C. (1998) A comparative summary of genetic distances in the vertebrates from the mitochondrial cytochrome b gene. Molecular Biology and Evolution, 15, 1481–1490.

    Köhler, G. (2012) Color Catalogue for Field Biologists. Herpeton, Offenbach, Germany, 49 pp.

    Kubicki, B. (2016) A new species of salamander (Caudata: Plethodontidae: Oedipina) from the central Caribbean foothills of Costa Rica. Mesoamerican Herpetology, 3, 819–840.

    Kubicki, B. & Arias, E. (2016) A beautiful new yellow salamander, genus Bolitoglossa (Caudata: Plethodontidae), from the northeastern slopes of the Cordillera de Talamanca, Costa Rica. Zootaxa, 4184 (2), 329–346.

    Lanfear, R., Calcott, B., Ho, S.Y.W. & Guindon, S. (2012) PartitionFinder: combined selection of partitioning schemes and substitution models for phylogenetic analyses. Molecular Biology and Evolution, 29, 1695-–1701.

    Meyer, C.P. (2003) Molecular systematics of cowries (Gastropoda: Cypraeidae) and diversification patterns in the tropics. Biological Journal of the Linnean Society, 79, 401–459.

    Meyer, C.P. & Paulay, G. (2005) DNA barcoding: error rates based on comprehensive sampling. PLoS biology, 3, e422.

    Miller, M.A., Pfeiffer, W. & Schwartz, T. (2010) Creating the CIPRES Science Gateway for inference of large phylogenetic trees. Proceedings of the Gateway Computing Environments Workshop (GCE), 1–8.

    Moritz, C., Schneider, C.J. & Wake, D.B. (1992) Evolutionary relationships within the Ensatina eschscholtzii complex confirm the ring species interpretation. Systematic Biology, 41, 273–291.

    Nicolas, V., Schaeffer, B., Missoup, A.D., Kennis, J., Colyn, M., Denys, C., Tatard, C., Cruaud, C. & Laredo, C. (2012) Assessment of three mitochondrial genes (16S, Cytb, CO1) for identifying species in the Praomyini tribe (Rodentia: Muridae). PLoS one, 7, e36586.

    Padial, J.M. & De La Riva, I. (2010) A response to recent proposals for integrative taxonomy. Biological Journal of the Linnean Society, 101, 747–756.

    Palumbi, S., Martin, A., Romano, S., McMillan, W.O., Stice, L. & Grabowski, G. (1991) The simple fool’s guide to PCR, version 2.0. Special Publication, Department of Zoology and Kewalo Marine Laboratory, University of Hawaii, Honolulu, Hawaii, USA.

    Rambaut, A., Suchard, M.A., Xie, W. & Drummond, A.J. (2014) Tracer v1.6.1.

    Ronquist, F., Teslenko, M., Van Der Mark, P., Ayres, D. L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M. & Huelsenbeck, J.P. (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic biology, 61, 539–542.

    Rovito, S.M., Parra-Olea, G., Hanken, J., Bonett, R.M. & Wake, D.B. (2013) Adaptive radiation in miniature: the minute salamanders of the Mexican highlands (Amphibia: Plethodontidae: Thorius). Biological Journal of the Linnean Society, 109, 622–643.

    Rovito, S.M., Parra-Olea, G., Recuero, E. & Wake, D.B. (2015) Diversification and biogeographical history of Neotropical plethodontid salamanders. Zoological Journal of the Linnean Society, 175, 167–188.

    Sambrook, J. & Russell, D.W. (2006) Purification of nucleic acids by extraction with phenol: chloroform. Cold Spring Harbor Protocols, pdb-prot 4455.

    Savage, J.M. (2002) The Amphibians and Reptiles of Costa Rica: A Herpetofauna between Two Continents, between Two Seas. The University of Chicago Press, Illinois, United States, 934 pp.

    Shen, X.X., Liang, D., Chen, M.Y., Mao, R.L., Wake, D.B. & Zhang, P. (2015) Enlarged multilocus data set provides surprisingly younger time of origin for the Plethodontidae, the largest family of salamanders. Systematic biology, 65, 66–81.

    Stamatakis, A. (2014) RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics, 30, 1312–1313.

    Stejneger, L. (1911) Description of three new batrachians from Costa Rica and Panama. Proceedings of the United States National Museum, 41, 285–288.

    Tamura, K. (1992) Estimation of the number of nucleotide substitutions when there are strong transition–transversion and G + C–content biases. Molecular Biology and Evolution, 9, 678–687.

    Tamura, K., Stecher, G., Peterson, D., Filipski, A. & Kumar, S. (2013) MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution, 30, 2725–2729.

    Taylor, E.H. (1948) New Costa Rican salamanders. Proceedings of Biological Society of Washington, 61, 177–180.

    Taylor, E.H. (1949) New salamanders of Costa Rica. University of Kansas Science Bulletin, 33, 279–288.

    Townsend, J.H. (2016) Taxonomic revision of the moss salamander Nototriton barbouri (Schmidt) (Caudata: Plethodontidae), with description of two new species from the Cordillera Nombre de Dios, Honduras. Zootaxa, 4196 (4), 511–528.

    Townsend, J.H., Butler, J.M., Wilson, L.D. & Austin, J.D. (2010) A distinctive new species of moss salamander (Caudata: Plethodontidae: Nototriton) from an imperiled Honduran endemism hotspot. Zootaxa, 2434, 1–16.

    Townsend, J.H., Medina-Flores, M., Murillo, J.L. & Austin, J.D. (2011) Cryptic diversity in Chortís Highland moss salamanders (Caudata: Plethodontidae: Nototriton) revealed using mtDNA barcodes and phylogenetics, with a new species from eastern Honduras. Systematics and Biodiversity, 9, 275–287.

    Townsend, J.H., Medina-Flores, M., Reyes-Calderón, O. & Austin, J.D. (2013) A new Nototriton (Caudata: Plethodontidae) from Parque Nacional Montaña de Botaderos in northeastern Honduras. Zootaxa, 3666 (3), 358–368.

    Wake, D.B. & Elias, P. (1983) New genera and a new species of Central American salamanders, with a review of the tropical genera (Amphibia, Caudata, Plethodontidae). Contributions in Science. Natural History Museum of Los Angeles County, 345, 1–19.

    Wake, D.B., Savage, J.M. & Hanken, J. (2007) Montane salamanders from the Costa Rica–Panamá border region, with descriptions of two new species of Bolitoglossa. Copeia, 2007, 556–565.[556:MSFTCR]2.0.CO;2