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Type: Article
Published: 2018-11-05
Page range: 145–150
Abstract views: 52
PDF downloaded: 1

A new record of Neoperla obliqua Banks, 1930 (Plecoptera: Perlidae) from Mt. Malindang, Mindanao, Philippines and association of life stages using DNA barcodes

Department of Life Science, National Taiwan Normal University, No. 88, Sec. 4, Tingzhou Rd., Taipei 11677, Taiwan Department of Biological Sciences, Mindanao State University—Iligan Institute of Technology, A. Bonifacio Ave., Tibanga, Iligan City 9200, Philippines Department of Biology, Caraga State University, Ampayon, Butuan City 8600, Philippines
Department of Biological Sciences, Mindanao State University—Iligan Institute of Technology, A. Bonifacio Ave., Tibanga, Iligan City 9200, Philippines
Department of Life Science, National Taiwan Normal University, No. 88, Sec. 4, Tingzhou Rd., Taipei 11677, Taiwan
DNA barcode Neoperla nymph ova Philippines Plecoptera

Abstract

The nymph of the perlid Neoperla obliqua Banks, 1913 from Mt. Malindang, Mindanao Island, the Philippines is described and associated with the male and female adults using DNA barcodes. Using pairwise CO1, the nymph was associated with 99.9 ± 0.14% interspecific similarity, while comparison between sexes generated a 0.2% intraspecific divergence between males and females of putative conspecifics. Additionally, the mature ova from female adults are described using scanning electron microscopy (SEM). The distinctive chorionic surface is rugose with longitudinal ridge-like pattern with a bare and flat collar.

 

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