Abstract
Paracobitis abrishamchiani, new species, is described from the southern Caspian Sea basin, where it is found in the Babol, Haraz, Siah, Tajan, Talar and Kashpal River drainages. It is distinguished from P. hircanica and P. atrakensis, the two other Paracobitis species known from the Caspian Sea basin, by having scales on the flank posterior to the dorsal-fin origin and from its congeners in the Middle East by the position of its dorsal-fin origin, having a roundish posterior narial opening, and a colour pattern consisting of many widely spaced brown spots and blotches. It is further characterized by six diagnostic nucleotide substitutions and a minimum K2P distance of 4.3% to P. persa and 4.6% to P. malapterura in the mtDNA COI barcode region.
References
Biomatters (2013) Geneious Pro. Available from: http://www.geneious.com (accessed 25 October 2018)
Edgar, R.C. (2004) MUSCLE: Multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research, 32, 1792–1797.
https://doi.org/10.1093/nar/gkh340
Esmaeili, H.R., Mousavi-Sabet, H., Sayyadzadeh, G., Vatandoust, S. & Freyhof, J. (2014) Paracobitis atrakensis, a new crested loach from northeast Iran (Teleostei: Nemacheiliidae). Ichthyological Exploration of Freshwaters, 25, 237–242.
Esmaeili, H.R., Pirvar, Z., Ebrahimi, M. & Geiger, M.F. (2015) Karyological and molecular analysis of three endemic loaches (Actinopterygii: Cobitoidea) from Kor River basin, Iran. Molecular biology research communications, 4 (1), 1.
Freyhof, J., Esmaeili, H.R., Sayyadzadeh, G. & Geiger, M. (2014) Review of the crested loaches of the genus Paracobitis from Iran and Iraq with the description of four new species (Teleostei: Nemacheilidae). Ichthyological Exploration of Freshwaters, 25, 11–38.
Freyhof, J., Geiger, M., Golzarianpour, K. & Patimar, R. (2016) Sasanidus, a new generic name for Noemacheilus kermanshahensis Bănărescu & Nalbant, with discussion of Ilamnemacheilus and Schistura (Teleostei; Nemacheilidae). Zootaxa, 4107 (1), 65–80.
https://doi.org/10.11646/zootaxa.4107.1.3
Hashemzadeh Segherloo, I., Ghaedrahmati, N. & Freyhof, J. (2016) Eidinemacheilus, a new generic name for Noemacheilus smithi Greenwood (Teleostei; Nemacheilidae). Zootaxa, 4147 (4), 466–476.
https://doi.org/10.11646/zootaxa.4147.4.7
Ivanova, N.V., Zemlak, T.S., Hanner R.H. & Hebert, P.D.N. (2007) Universal primer cocktails for fish DNA barcoding. Molecular Ecology Notes, 7, 544–548.
https://doi.org/10.1111/j.1471-8286.2007.01748.x
Kapli, P., Lutteropp, S., Zhang, J., Kobert, K., Pavlidis, P., Stamatakis, A. & Flouri, T. (2017) Multi-rate Poisson Tree Processes for single-locus species delimitation under Maximum Likelihood and Markov Chain Monte Carlo. Bioinformatics, 33 (11), 1630–1638.
https://doi.org/10.1093/bioinformatics/btx025.
Kimura, M. (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of molecular evolution, 16 (2), 111–120.
https://doi.org/10.1007/BF01731581
Kottelat, M. & Freyhof, J. (2007) Handbook of European freshwater fishes. Kottelat, Cornol and Freyhof, Berlin, xiv + 646 pp.
Kottelat, M. (1990) Indochinese nemacheilines. A revision of nemacheiline loaches (Pisces: Cypriniformes) of Thailand, Burma, Laos, Cambodia and southern Viet Nam. Verlag Dr. Friedrich Pfeil, München, 262 pp.
Masters, B.C., Fan, V. & Ross, H.A. (2011) Species delimitation—a geneious plugin for the exploration of species boundaries. Molecular Ecology Resources, 11, 154–157.
https://doi.org/10.1111/j.1755-0998.2010.02896.x
Mousavi-Sabet, H., Sayyadzadeh, G., Esmaeili, H.R., Eagderi, S., Patimar, R. & Freyhof, J. (2014) Paracobitis hircanica, a new crested loach from the southern Caspian Sea basin (Teleostei: Nemacheilidae). Ichthyological Exploration of Freshwaters, 25, 237–242.
Posada, D. & Crandall, K.A. (1998) MODELTEST: testing the model of DNA substitution. Bioinformatics, 14, 817–818.
https://doi.org/10.1093/bioinformatics/14.9.817
Saitou, N. & Nei, M. (1987) The Neighbor-Joining Method – a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution, 4, 406–425
Swofford, D.L. (2002) PAUP*. Phylogenetic analysis using parsimony (*and other methods). Version 4. Sinauer Associates, Sunderland, Massachusetts. [software]
Zhang, J., Kapli, P., Pavlidis, P. & Stamatakis, A. (2013) A general species delimitation method with applications to phylogenetic placements. Bioinformatics, 29 (22), 2869–2876.
https://doi.org/10.1093/bioinformatics/btt499