Abstract
Three new entodiniomorphid species, Buetschlia minuta n. sp., Buetschlia cirrata n. sp., and Charonina elephanti n. sp., were described from the hindgut of Asian elephants (Elephas maximus) from Gaziantep, Turkey. B. minuta n. sp. has an ovoid body shape with a truncated anterior end and a rounded posterior end, an adoral ciliary zone surrounding the cytostome, somatic ciliary rows in the anterior two thirds of the body, an ovoid macronucleus without a constant position, and a concretion vacuole in the anterior one third of the body. B. cirrata n. sp. has an ovoid body shape with the anterior end truncated and the posterior end rounded, an adoral ciliary zone surrounding the cytostome, unevenly distributed somatic cilia, an ovoid macronucleus without a constant position, and a concretion vacuole in the anterior one third of the body. C. elephanti n. sp. has an ovoid body shape with both ends rounded, an ovoid macronucleus without a constant position, two buccal ciliary zones, an adoral ciliary zone, a vestibular ciliary zone, three somatic ciliary zones, a dorsal ciliary zone, two posterior ciliary zones, dorsal and ventral, and a vestibulum with a Y-shaped infraciliature.
References
Anderson, E. & Dumont, J. (1966) A comparative study of the concrement vacuole of certain endocommensal ciliates-a so-called mechanoreceptor. Journal of Ultrastructure Research, 15, 414–450.
https://doi.org/10.1016/S0022-5320(66)80117-X
Bretschneider, L.H. (1962) Das paralabialorgan der ophryoscoleciden. Proceedings of the Academy of Sciences Amsterdam, Series C, 65, 423–437.
Curds, C.R. (1985) The species problem in ciliates – a taxonomist’s view. Atti della Societa Toscana di Scienza Naturali, Memorie Serie B, 92, 29–41.
Dehority, B.A. (1986) Protozoa of the digestive tract of herbivorous mammals. Insect Science and Its Application, 7, 279–296.
https://doi.org/10.1017/S1742758400009346
Dehority, B.A. (1994) Rumen ciliate protozoa of the blue duiker (Cephalophus monticola), with observations on morphological variation lines within the species Entodinium dubardi. Journal of Eukaryotic Microbiology, 41, 103–111.
https://doi.org/10.1111/j.1550-7408.1994.tb01481.x
Dehority, B.A. (2006) Studies on the variation in caudal spination of Epidinium. Zootaxa, 1305, 33–39.
Dogiel, V.A. (1928) La faune d’infusoires habitant l’estomac du buffle et du dromadaire. Annales de Parasitologie Humaine et Comparee, 6, 323–338.
https://doi.org/10.1051/parasite/1928063323
Dogiel, V.A. (1929) Die sog. “Konkrementenvakuole” der infusoiren al eine statocyste betrachtet. Archiv für Protistenkunde, 63, 319–348.
Eloff, A.K. & Van Hoven, W. (1980) Intestinal protozoa of the African elephant Loxodonta africana (Blumenbach). South African Journal of Zoology, 15, 83–90.
https://doi.org/10.1080/02541858.1980.11447691
Fiorentini, A. (1890) Sur les protistes de l’estomac de bovidés. Journal de Micrographie, 14, 23–28 + 79–83 + 178–183.
Gilchrist, F.M.C., Van Hoven, W. & Stenson, M.O. (1994) Five new species of Trichostomatida (ciliated protozoa) from the colon of wild African rhinoceroses. Systematic Parasitology, 28, 187–196.
https://doi.org/10.1007/BF00009517
Gürelli, G. (2016) Intestinal ciliate fauna of the Asian elephant from Gaziantep, Turkey and the description of Brevitentaculum antebum n. g., n. sp. European Journal of Protistology, 56, 26–31.
https://doi.org/10.1016/j.ejop.2016.05.001
Gürelli, G. (2017) Rumen Ciliate Fauna of Domestic Sheep (Ovis aries) in İzmir, Turkey and Scanning Electron Microscopic Observations. Zootaxa, 4286 (4), 545–554.
https://doi.org/10.11646/zootaxa.4286.4.7
Gürelli, G. & Ito, A. (2014) Intestinal ciliated protozoa of the Asian elephant Elephas maximus Linnaeus, 1758 with the description of Triplumaria izmirae n. sp. European Journal of Protistology, 56, 25–32.
https://doi.org/10.1016/j.ejop.2013.10.002
Hsiung, T.-S. (1930) A monograph on the protozoa of the large intestine of the horse. Iowa State College Journal of Science, 4, 359–423.
Hsiung, T.-S. (1932) A general survey of the protozoan fauna of the rumen of Chinese cattle. Bulletin of the Fan Memorial Institute of Biology, 3, 87–103.
Imai, S., Tsutsumi, Y., Yumura, S. & Mulenga, A. (1992) Ciliate protozoa in the rumen of Kafue lechwe, Kobus leche kafuensis, in Zambia, with the description of four new species. Journal of Protozoology, 39, 564–572.
https://doi.org/10.1111/j.1550-7408.1992.tb04852.x
Ito, A. & Imai, S. (1998) Infraciliary bands in the rumen ophryoscolecid ciliate Ostracodinium gracile (Dogiel, 1925), observed by light microscopy. Journal of Eukaryotic Microbiology, 45, 628–636.
https://doi.org/10.1111/j.1550-7408.1998.tb04559.x
Ito, A. & Imai, S. (2003) Light microscopical observation of infraciliary bands of Eodinium posterovesiculatum in comparison with Entodinium bursa and Diplodinium dentatum. Journal of Eukaryotic Microbiology, 50, 34–42.
https://doi.org/10.1111/j.1550-7408.2003.tb00103.x
Ito, A. & Imai, S. (2006) Infraciliary band pattern of rumen ophryoscolecid ciliates. Endocytobiosis and Cell Research, 17, 103–110.
Ito, A., Ishihara, M. & Imai, S. (2014) Bozasella gracilis n. sp. (Ciliophora, Entodiniomorphida) from Asian elephant and phylogenetic analysis of entodiniomorphids and vestibuliferids. European Journal of Protistology, 50, 134–152.
https://doi.org/10.1016/j.ejop.2014.01.003
Ito, A., Mishima, T., Nataami, K., Ike, K. & Imai, S. (2011) Infraciliature of eight Triplumaria species (Ciliophora, Entodiniomorphida) from Asian elephants with the description of six new species. European Journal of Protistology, 47, 256–273.
https://doi.org/10.1016/j.ejop.2011.06.002
Ito, A., Yutaka, M. & Imai, S. (2001) Light microscopic observations of infraciliature and morphogenesis in six species of rumen Ostracodinium ciliates. Journal of Eukaryotic Microbiology, 48, 440–448.
https://doi.org/10.1111/j.1550-7408.2001.tb00177.x
Ito, A., Yutaka, M. & Imai, S. (2002) Descriptions of new Parentodinium ciliates in the family Parentodiniidae n. fam. from Hippopotamus amphibius in comparison with some entodiniomorphs from horses and cattle. European Journal of Protistology, 37, 405–426.
https://doi.org/10.1078/0932-4739-00828
Jameson, A.P. (1925) A new ciliate, Charon ventriculi n. g., n. sp. from the stomach of ruminants. Parasitology, 17, 403–405.
https://doi.org/10.1017/S0031182000004807
Kofoid, C.A. (1935) On two remarkable ciliate protozoa from the caecum of the Indian elephant. Proceedings of the National Academy of Sciences, 21, 501–506.
https://doi.org/10.1073/pnas.21.7.501
Kornilova, O.A. (2004) History of Study of Endobiotic Ciliates of Mammalia. Tessa Press, St-Petersburg, 349 pp.
Latteur, B. (1958) Les ciliates Polydiniinae Thoracodinium vorax n. gen., n. sp. Cellule, 59, 271–296.
Latteur, B. (1967) Helicozoster indicus n. gen, n. sp. ciliate holotriche du caecum de l’éléphant des Indes. Acta Zoologica et Pathologica Antverpiensia, 43, 93–103.
Latteur, B. & Bousez, M.P. (1970) Rhabdothorax macrostegon n. gen., n. sp. cilié Cycloposthiidae du colon de l’éléphant d’Asie. Annales de la Société Royale Zoologique de Belgique, 99, 193–214.
Latteur, B., Tuffrau, M. & Wespes, G. (1970) Triplumaria selenica n. sp. cilié spirotriche du colon de l’éléphant d’Afrique. Protistologica, 6, 319–327.
Lynn, D.H. (2008) The Ciliated Protozoa, Characterization, Classification and Guide to the Literature. Springer, Dordrecht, 605 pp.
Mandal, D. & Choudhury, A. (1983) On two new cycloposthiid ciliates from Indian elephant Elephas maximus L. Journal of the Bengal Natural History Society, 2, 13–18.
Miltko, R., Michalowski, T., Pristas, P., Javorsky, P. & Hackstein, J.H.P. (2006) Factors influencing morphological variability of rumen ciliates from the genus Ophryoscolex. Journal of Animal and Feed Sciences, 15, 35–38.
https://doi.org/10.22358/jafs/70137/2006
Moon-van der Staay, S.Y., van der Staay, G.W.M., Michalowski, T., Jouany, J.-P., Pristas, P., Javorský, P., Kišidayová, S., Varadyova, Z., McEwan, N.R., Newbold, C.J., van Alen, T., de Graaf, R., Schmid, M., Huynen, M.A. & Hackstein, J.H.P. (2014) The symbiotic intestinal ciliates and the evolution of their hosts. European Journal of Protistology, 50, 166–173.
https://doi.org/10.1016/j.ejop.2014.01.004
Ogimoto, K. & Imai, S. (1981) Atlas of Rumen Microbiology. Japan Scientific Societies Press, Tokyo, 231 pp.
Regensbogenova, M., Svetlana, K., Michalowski, T., Javorsky, P., Moon-van der Staay, S.Y., Moon-van der Staay, G.W.M., Hackstein, J.H.P., McEwan, N.R., Jouany, J.-P., Newbold, J.C. & Pristas, P. (2004) Rapid identification of rumen protozoa by restriction analysis of amplified 18S rRNA gene. Acta Protozoologica, 43, 219–224.
Rossi, M.F., Dias, R.J.P., Senra, M.V.X., Martinele, I., Soares, C.A.G. & D’Agosto, M. (2015) Molecular phylogeny of the family Ophryoscolecidae (Ciliophora, Litostomatea) inferred from 18S rDNA Sequences. Journal of Eukaryotic Microbiology, 62, 584–590.
https://doi.org/10.1111/jeu.12211
Schmidt, S.L., Bernhard, D., Schlegel, M. & Fried, J. (2006) Fluorescence in situ hybridization with specific oligonucleotide rRNA probes distinguishes the sibling species Stylonychia lemnae and Stylonychia mytilus (Ciliophora, Spirotrichea). Protist, 157, 21–30.
https://doi.org/10.1016/j.protis.2005.09.004
Schrenk, H.-G. & Bardele, C.F. (1987) The fine structure of the paralabial organelle in the rumen ciliate Ophryoscolex purkinjei Stein, 1858. Journal of Protozoology, 34, 97–104.
https://doi.org/10.1111/j.1550-7408.1987.tb03141.x
Schuberg, A. (1888) Die protozoen des wiederkäuermagens. 1. Butschlia, Isotricha, Dasytricha, Entodinium. Zoologische Jahrbuecher, 3, 365–418.
Strelkow, A. (1939) Parasitological infusoria from the intestine of Ungulata belonging to the family Equidae. Uchenye Zapiski Leningrad Pedagog Institute Gertsena, 17, 1–262.
Thurston, J.P. & Grain, J. (1971) Holotrich ciliates from the stomach of Hippopotamus amphibious, with descriptions of two new genera and four new species. Journal of Protozoology, 18, 133–141.
https://doi.org/10.1111/j.1550-7408.1971.tb03295.x
Timoshenko, O. & Imai, S. (1995) Eleven new species of the genus Triplumaria (Entodiniomorphida) from Asian elephant, Elephas maximus and African elephant Loxodonta africana. Journal of Protozoology Research, 5, 157–175.
Timoshenko, O. & Imai, S. (1997) Three new intestinal protozoan species of the genus Latteuria n. g. (Ciliophora: Trichostomatia) from Asian and African elephants. Parasitology International, 46, 297–303.
https://doi.org/10.1016/S1383-5769(97)00040-8
Vďačný, P. (2018) Evolutionary associations of endosymbiotic ciliates shed light on the timing of the marsupial-placental split. Molecular Biology and Evolution, 35 (7), 1757–1769.
https://doi.org/10.1093/molbev/msy071
Williams, A.G. & Coleman, G.S. (1992) The Rumen Protozoa. Springer, New York, 442 pp.
https://doi.org/10.1007/978-1-4612-2776-2
Wolska, M. (1964) Infraciliature of Didesmis ovalis Fior. and Blepharozoum trizonum (Hsiung)-fam. Buetschliidae (Ciliata, Rhabdophorina). Acta Protozoologica, 2, 153–158.
Wolska, M. (1967a) Study on the family Blepharocorythidae Hsiung. III. Raabena bella gen. n., sp. n. from the intestine of the Indian elephant. Acta Protozoologica, 4, 284–290.
Wolska, M. (1967b) Study on the family Blepharocorythidae Hsiung. II. Charonina ventriculi (Jameson). Acta Protozoologica, 4, 279–283.
Wolska, M. (1968) Study on the family Blepharocorythidae Hsiung. IV. Pararaabena dentata gen. n., sp. n. from the intestine of the Indian elephant. Acta Protozoologica, 5, 219–224.
Wolska, M. (1970) Spirocorys indicus Wolska, 1969 a ciliate from the intestine of Indian elephant, its systematic position. Acta Protozoologica, 7, 143–148.
Wolska, M. (1971a) Studies on the family Blepharocorythidae Hsiung. V. A review of genera and species. Acta Protozoologica, 9, 23–40.
Wolska, M. (1971b) Studies on the family Blepharocorythidae Hsiung. VI. Phylogenesis of the family and the description of the new genus Circodinium gen. n. with the species C. minimum (Gassovsky, 1918). Acta Protozoologica, 9, 171–194.
Wolska, M. (1986) Pseudoentodinium elephantis gen. n., sp. n. from the order Entodiniomorphida. Proposition of the new family Pseudoentodiniidae. Acta Protozoologica, 25, 139–146.
Wright, A.-D.G. (1999) Analysis of intraspecific sequence variation among eight isolates of the rumen symbiont, Isotricha prostoma (Ciliophora), from two continents. Journal of Eukaryotic Microbiology, 46 (4), 445–446.
https://doi.org/10.1111/j.1550-7408.1999.tb04627.x