Abstract
Aphanolaimus strilliae n. sp. and Makatinus africanus n. sp. are described from freshwater sources in the Telperion Nature Reserve, Mpumalanga, South Africa. Aphanolaimus strilliae n. sp. is characterised by a body length of 1240–1613 μm, more than 800 body annules, lateral field originating between the first and second lateral body pore at the 34th–46th annule, first lateral body pore located at the 25th–35th annule, vagina V-shaped and bent anteriorly, 142–195 µm long uterus and 165–207 µm long tail with spinneret offset. This species is ovoviviparous and no males were found. Makatinus africanus n. sp. is characterised by a large, thick body (3228–4128 μm long, a = 30–39 wide), slightly set off lip region with amalgamated lips; 31–34 μm long odontostyle; long tongue-shaped cardia, and stout, short tail (30–43 μm long) with a small peg / digitate extension and male absent. Populations of three known species, Chronogaster africana, Eutobrilus annetteae and Neotobrilus ampiei, from fresh water at the Telperion Nature Reserve are described and scanning electron microscope graphs of these species published for the first time.
References
Ahmad, W. (1997) Observations on the genus Makatinus Heyns, 1965 (Aporcelaimidae: Dorylaimida) with comments on the status of Aporcelaimus minor Loos, 1945. Pakistan Journal of Nematology, 15 (1/2), 1–5.
Ahmad, W. & Ahmad, I. (1992) Makatinus heynsi n. sp. (Dorylaimida: Aporcelaimidae) from Goa, India. Fundamental and Applied Nematology, 15 (2), 149–152.
Ahmad, W., Sturhan, D. & Wouts, W.M. (2003) Four new species of Dorylaimida (Nematoda) from New Zealand. New Zealand Journal of Nematology, 30 (1), 43–55.
https://doi.org/10.1080/03014223.2003.9518323
Andrássy, I. (1964) Ein Versuchsschlüssel zur Bestimmung der Tobrilus–Arten. Annales Universitatis Scientiarum Budapestinensis, 7, 3–18
Andrássy, I. (1970) Nematoden aus einigen Fluss–Systemen Südafrikas. Opuscula Zoologica Instituti Zoosystematici et Oecologici Universitatis Budapestinensis, 10, 179–219.
Andrássy, I. (1986) The genus Eudorylaimus Andrássy, 1959 and the present status of its species (Nematoda: Qudsianematidae). Opuscula Zoologica Instituti Zoosystematici et Oecologici Universitatis Budapestinensis, 22, 1–42.
Andrássy, I. (2005) Free–living nematodes of Hungary (Nematoda errantia), Volume I, Hungarian Natural History Museum & Systematic Zoology Research Group of the Hungarian Academy of Sciences, Budapest, 518 pp.
Andrássy, I. (2007) Free–living nematodes of Hungary, (Nematoda Errantia) Volume II. Hungarian Natural History Museum & Systematic Zoology Research Group of the Hungarian Academy of Sciences, Budapest, 496 pp.
Andrássy, I. (2009) Free–living nematodes of Hungary, (Nematoda Errantia) Volume III. Hungarian Natural History Museum & Systematic Zoology Research Group of the Hungarian Academy of Sciences, Budapest, 608 pp.
Argo, A.D. & Heyns, J. (1973) New and little known species of the nematode families Monhysteridae and Tripylidae from South Africa. Phytophylactica, 5, 149–154.
Botha, A. & Heyns, J. (1992) Further records and descriptions of nematodes from rivers in the Kruger National Park (orders Enoplida, Chromadorida, Monhysterida, Mononchida and Araeolaimida). Koedoe, 35 (2), 11–25.
https:// doi.org/10.4102/koedoe.v35i2.401
Cobb, N.A. (1913) New nematode genera found inhabiting fresh water and non–brackish soils. Journal of the Washington Academy of Science, 3, 432–444.
https://doi.org/10.5962/bhl.part.20323
Cobb, N.A. (1914) The North American free–living freshwater nematodes. Transactions of American Microscopical Society, 33 (2), 70–118.
https://doi.org/10.2307/3221617
Coetzee, V. (1965) South African species of the genus Cobbonchus Andrássy, 1958 (Nematoda: Mononchidae). Nematologica, 11 (2), 281–290.
https://doi.org/10.1163/187529265X00186
Coetzee, V. (1966) Species of the genera Granonchulus and Cobbonchus (Mononchidae) occurring in southern Africa. Nematologica, 12 (2), 302–312.
https://doi.org/10.1163/187529266X00725
Coetzee, V. (1967a) Species of the genus Mylonchulus (Nematoda: Mononchidae) occurring in southern Africa. Nematologica, 12 (4), 557–567.
https://doi.org/10.1163/187529266X00392
Coetzee, V. (1967b) Species of the genus Iotonchus (Nematoda: Mononchidae) occurring in southern Africa. Nematologica, 13 (3), 367–377.
https://doi.org/10.1163/187529267X00599
Coetzee, V. (1968a) Southern Africa species of the genera Mononchus and Prionchulus (Mononchidae). Nematologica, 14 (1), 63–76.
https://doi.org/10.1163/187529268X00651
Coetzee, V. (1968b) Mononchidae (Nematoda) of southern Africa. South African Journal of Agricultural Science, 11 (2), 403–414.
https://doi.org/10.1163/187529268X00651
Coomans, A. & De Waele, D. (1983) Species of Aphanolaimus (Nematoda: Araeolaimida) from Belgium. Hydrobiologia, 101 (3), 165–178.
https://doi.org/10.1007/BF00009872
Daday, J. (1894) Újabb adatok a Balaton mikrofaunájának ismeretéhez. Mathematikai és Természettudományi Értesítő, 12, 122–145.
Daday, E. (1905) Untersuchungen über die Süsswasser–Mikrofauna Paraguays. Zoologica, Stuttgart, 18 (44), 1–374.
https://doi.org/10.5962/bhl.title.11316
Danovaro, R., Gambi, C., Höss, S., Mirto, S., Traunspurger, W. & Zullini, A. (2009) Case studies using nematode assemblage analysis in aquatic habitats. In: Wilson, M.J. & Kakouli–Duarte, T. (Eds.), Nematodes as environmental indicators. CAB International, Wallingford, pp. 146–171.
https://doi.org/10.1079/9781845933852.0000
Dassonville, A.F. (1981) ’n Taksonomie en ekologiese studie van varswater–nematode in Skinnerspruit, Pretoria. Unpublished Ph.D. Thesis, Rand Afrikaans University, Johannesburg.
de Man, J.G. (1880) Die einheischen, frei in der reinen Erde und im süssen Wasser lebenden Nematoden. Vorläufiger Bericht und descriptivsystematischer Theil. Tijdschrift der Nederlandsche Dierkundige Vereeniging, 5, 1–104.
de Man, J.G. (1884) Die Frei in der reinen Erde und in süssen Wasser lebenden Nematoden der Niederländishen Fauna, Eine systematische–faunistische Monographie. Brill, Leiden.
https://doi.org/10.5962/bhl.title.46884
De Waele, D. & Coomans, A.V. (1993) Four new species of Aphanolaimus de Man, 1880 (Nematoda: Araeolaimida) from South America. Fundamental and Applied Nematology, 16 (1), 87–95.
Eyualem-Abebe & Coomans, A. (1995) Freshwater nematodes of the Galápagos. Hydrobiologia, 299 (1), 1–51.
Eyualem-Abebe, Decraemer, W. & De Ley, P. (2008) Global diversity of nematodes (Nematoda) in freshwater. Hydrobiologia, 595, 67–78.
Eyualem-Abebe, Mees, J. & Coomans, A. (2001) Nematode communities of Lake Tana and other inland water bodies of Ethiopia. Hydrobiologia, 462 (1–3), 41–73.
https://doi.org/10.1023/A:1013176117586
Eyualem-Abebe, Traunspurger, W. & Andrássy, I. (2006) Freshwater nematodes: ecology and taxonomy. CAB International: Wallingford, 752 pp.
https://doi.org/10.1079/9780851990095.0000
Goodey, T. (1963) Soil and freshwater nematodes (2nd edition), revised and rewritten by Goodey, J.B. Methuen and Co, London, 544 pp.
Grobler, A. (1999) Phytosociology & veld condition assessment of Ezemvelo Game Reserve. Unpublished B.Sc. Honours thesis, University of Pretoria, Pretoria.
Heyns, J. (1965) On the morphology and taxonomy of the Aporcelaimidae, a new family of dorylaimoid nematodes. Entomology Memoirs, Department of Agriculture Technical Services. Republic of South Africa, 10, 1–51.
Heyns, J. (2002a) Checklist of free living nematodes recorded from freshwater habitats in southern Africa. Water SA, 28 (4), 449–456.
https://doi.org/10.4314/wsa.v28i4.4918
Heyns, J. (2002b) Nematoda. In: Day, J.A. & De Moor, I.J. (Eds.), Guides to the freshwater invertebrates of southern Africa, Volume 5: Non–Arthropods. Water Research Commission, Pretoria, pp. 136–161.
Heyns, J. & Coomans, A. (1980) Freshwater nematodes from South Africa 5. Chronogaster Cobb, 1913. Nematologica, 26 (2), 187–208.
https://doi.org/10.1163/187529280X00080
Hirschmann, H. (1983) Scanning electron microscopy as a tool in nematode taxonomy. In: Stone, A.R., Platt, H.M. & Khall, L.F. (Eds.), Concepts in nematode systematics. The Systematics Association special volume No 22. Academic Press, London, pp. 95–111.
Höfmanner, R. & Menzel, R. (1914) Neue Arten freilebenden Nematoden aus der Schweiz. Zoologischer Anzeiger, 44, 80–91.
Holovachov, O. & De Ley, P. (2006) Order Plectida In: Eyualem–Abebe, Traunspurger, W. & Andrássy, I., (Eds), Freshwater nematodes: Ecology and taxonomy. CAB International, Wallingford, pp. 611–647.
https://doi.org/10.1079/9780851990095.0611
Jägerskiöld, L.A. (1909) Freilebende Süßwassernematoden. In: Brauer, Die Süßwasserfauna Deutschlands, Heft 15 Nematodes, Mermithidae und Gordiidae. Verlag von Gustav, Jena, 92 pp.
Jiménez-Guirado, D. (1994) Observations of the genus Makatinus Heyns, 1965 (Nematoda: Aporcelaimidae) and description of M. aquaticus sp. n. from Spain. Afro–Asian Journal of Nematology, 4 (1), 1–6.
Joubert, A.P. & Heyns, J. (1979) Freshwater nematodes from South Africa. 3. Tobrilus Andrássy, 1959. Journal of the Limnological Society of Southern Africa, 5 (1), 17–26.
https://doi.org/10.1080/03779688.1979.9633176
Kreis, H.A. (1924) Contribution á la connaissance des Nématodes libres du Surinam (Guyane Hollandaise). Annales de Biologie Lacustre, 13 (1–2), 123–136.
Leidy, M.D. (1852) Helminthological contributions. Proceediпgs of the Aсаdеmу of Natural Sсiепсеs. Philadelphia, 5, 224 –227.
Loof, P.A.A. & Riemann, F. (1976) Taxonomy of the Tobrilus longus group (Enoplida: Tripylidae). Nematologica, 22 (1), 15–48.
https://doi.org/10.1163/187529276X00030
Loos, C.A. (1945) Notes on free–living and plant–parasitic nematodes of Ceylon. 1. Ceylon. Journal of Science. Section B. Zoology and Geology, 23 (1), 1–7.
Marais, M., Swart, A., Fourie, H., Berry, S.D., Knoetze, R. & Malan, A.P. (2017) Techniques and procedures. In: Fourie, H., Spaull, V.W., Jones, R.K., Daneel, M.S. & De Waele, D. (Eds.), Nematology in South Africa: A view from the 21st century. Springer International Publishing, pp. 73–118.
https://doi.org/10.1007/978-3-319-44210-5_4
Micoletzky, H. (1922) Die freilebenden Erd–Nematoden mit besonderer Berücksichtigung der Steiermark und der Bukowina, zugleich mit einer Revision sämtlicher, nicht mariner, freilebender Nematoden in Form von Genus–Beschreibungen und Bestimmungsschlusseln. Archiv für Naturgeschichte Berlin Abteilung A, 87 (8), 1–649.
Micoletzky, H. (1925) Die freilebenden Süsswasser– und Moornematoden Dänemarks. Det Kongelige Danske Videnskabberns Selskab Kobenhavn, Series 8, 210, 57–310.
Mobara, A. (2014) A taxonomical and ecological study of nematodes from the Seekoeivlei Nature Reserve, Memel (Unpublished Master’s thesis). University of the Free State, Bloemfontein.
Mucina, L., Hoare, D.B., Lötter, M.C., Rutherford, M.C., Scott–Shaw, C.R., Bredenkamp, G.J., Powrie, L.W., Scott, L., Camp K.G.T., Cilliers, S.S., Bezuidenhout, H., Mostert, T.H., Siebert, S.J., Winter, P.J.D., Burows, J.E., Dobson, L., Ward, R.A., Stalmans, M., Oliver, E.G.H., Sibert, F., Schmidt, E., Kobisi, K. & Kose, L. (2006) Grassland Biome. In: Mucina, L. & Rutherford, M.C.& Rutherford, M.C. (Eds.), The vegetation of South Africa, Lesotho and Swaziland, Strelitzia 19. South African National Biodiversity Institute, Pretoria, pp. 349–436.
Peña-Santiago, R. & Varela, I. (2017) Taxonomy and systematics of the genus Makatinus Heyns, 1965 (Nematoda: Dorylaimida: Aporcelaimidae). Journal of Nematology, 49 (3), 245–253.
Plotnikov, W. (1899) Nematoda, Oligochaeta u. Hirudines. Arb. D. Naturforscherges. St. Petersburg, Bd. XXX
Raski, D.J. & Coomans, A. (1990) Five new species of Aphanolaimus (Nemata: Araeolaimida) with a key to the species. Nematologica, 36 (1), 22–54.
https://doi.org/10.1163/002925990X00022
Schneider, G. (1916) Synopsis öfver de I östra, norra och mellersta Europas farskvatten fritt lefvande nematoderna. Acta Societatis pro Fauna et Flora Fennica, 44, 1–83.
Schneider, G. (1922) Das mannchen von Aphanolaimus viviparus Plotnikov. Sitzungsberichte der Naturforscher–Gesellschaft Dorpat, 28, 22–23.
Shorthouse, D.P. (2010) SimpleMappr, an online tool to produce publication–quality point maps. Available from: http://www.simplemappr.net (accessed 20 November 2017)
Swart, A. & Heyns, J. (1988) Redescription of Eutobrilus heptapapillatus (Joubert and Heyns, 1979) Tsalolikhin, 1981 with notes on its morphology and a possible excretory system (Nematoda: Tobrilidae). Phytophylactica, 20, 161–168.
Traunspurger, W. (1989) Systematik und Ökologie der Nematoda des Königssees. (Unpublished doctoral thesis). Ludwig Maximilians-University of Munich, 305 pp.
Traunspurger, W. (2000) The biology and ecology of lotic nematodes. Freshwater Biology, 44 (1), 29–45.
Tsalolikhin, S.Ya. (1981) Revision of the genus Tobrilus (Nematoda–Tobrilidae). Zoologicheskii Zhurnal, 60, 1302–1313. [in Russian]
Тsаlоlikhin, S.Ya. (1983) Nematodes from families Tobrilidae аnd Tipylidae of all world fаunа. Leningrad: Nauka, 232 pp. [in Russian]
Tsalolikhin, S.Ya. & Shoshin, A.V. (2009) Rеviеw of the genus Neotobrilus Tsalolikhin, 1981 (Nematoda, Enoplida: Tobrilidae). Russian Journal of Nematology, 17 (1), 59–72.
Yunliang, P. & Coomans, A. (2000) Three species of Tobrilidae (Nematoda: Enoplida) from Li river at Guiling China. Hydrobiologia, 421 (1), 77–90.
Zullini, A. (1988) A new genus and five species of nematodes from Ethiopian lakes. Revue de Nématologie, 11 (3), 279–288.