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Type: Article
Published: 2019-12-09
Page range: 201–230
Abstract views: 62
PDF downloaded: 56

Taxonomic notes on the Ectoedemia suberis and angulifasciella species groups in Japan (Lepidoptera: Nepticulidae) 

Entomological Laboratory, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka, 819-0395 Japan.
3-9955, Hata, Matsumoto-shi, Nagano, 390-1401 Japan.
Entomological Laboratory, Faculty of Agriculture, Kyushu University, 744 Motooka.
Lepidoptera Ectoedemia chasanella Ectoedemia ortiva female genitalia Quercus taxonomy


Diagnoses and notes on biology and distribution of Japanese species of the suberis and angulifasciella groups of the genus Ectoedemia Busck, 1907 are given. Two species, E. chasanella Puplesis, 1984 and E. ortiva Rocienė & Stonis, 2013, are reported for the first time from Japan and their morphology is redescribed. In addition, we provide molecular identification of the two species and their relatives based on COI barcode sequences and the nuclear gene (EF1-α) sequences. The female of E. chasanella is described for the first time. We confirm that E. chasanella utilizes Quercus species (Fagaceae) as its hostplant: Q. dentata, Q. serrata, Q. crispula, and Q. acutissima. Ectoedemia ortiva was formerly placed in the suberis group, and its host plant was unknown. We move this species to the angulifasciella group and provide evidence that it utilizes Ulmus sp. (Ulmaceae) as its host plant.



  1. Akaike, H. (1974) A new look at the statistical model identification. IEEE Transactions on Automatic Control, 19, 716–723.

    Doorenweerd, C., van Nieukerken, E.J. & Menken, S.B.J. (2015) A global phylogeny of leafmining Ectoedemia moths (Lepidoptera: Nepticulidae): exploring host plant family shifts and allopatry as drivers of speciation PLoS ONE, 10, 1–20.

    Doorenweerd, C., van Nieukerken, E.J. & Hoare, R.J.B. (2017) Phylogeny, classification and divergence times of pygmy leafmining moths (Lepidoptera: Nepticulidae): the earliest lepidopteran radiation on Angiosperms? Systematic Entomology, 42 (1), 267–287.

    Hajibabaei, M., Janzen, D.H., Burns, J.M., Hallwachs, W. & Hebert, P.D.N. (2006) DNA barcodes distinguish species of tropical Lepidoptera. Proceedings of the National Academy of Sciences of the United States of America, 103, 968–971.

    Hebert, P.D., Cywinska, A., Ball, S.L. & deWaard, J.R. (2003) Biological identifications through DNA barcodes. Proceedings of the Royal Society of London, Series B: Biological Sciences, 270 (1512), 313–321.

    Hirano, N. (2009) Nepticulidae. In: Arita, Y. (Ed.), Smaller moths of the Nasu Imperial Villa, Tochigi-ken, Japan. Flora and Fauna of the Nasu Imperial Villa, II, pp. 50–51 + 108–109. [in Japanese]

    Hirano, N. (2013) Nepticulidae. In Hirowatari, T., Nasu, Y., Sakamaki, Y. & Kishida, Y. (Eds.), The standard of moths in Japan III. Gakken Education Publishing, Tokyo, pp. 80–96, pls. 14–16. [in Japanese]

    Hirano, N. (2014) Six new and an unrecorded species of Stigmella Schrank, 1802 from Japan (Lepidoptera, Nepticulidae). Tinea, 23 (1), 19–32. [in Japanese]

    Johansson, R., Nielsen, E.S., van Nieukerken, E.J. & Gustafsson, B. (1990) The Nepticulidae and Opostegidae (Lepidoptera) of north west Europe. Fauna Entomologica Scandinavica, 23 (2), 1–739.

    Kumar, S., Stecher, G. & Taamura, K. (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution, 33 (7), 1870–1874.

    Linnaeus, C. (1767) Systema naturae. Tom. I. Pars II. Editio duodecima reformata. Holmiaej, 796 pp. [pp. 533–1328 + 1–1336]

    Morgulis, A., Coulouris, G., Raytselis, Y., Madden, T.L., Agarwala, R. & Schäffer, A.A. (2008) Database indexing for production MegaBLAST searches. Bioinformatics, 24 (16), 1757–1764.

    Okaura, T., Quang, N.D., Ubukata, M. & Harada, K. (2007) Phylogeographic structure and late Quaternary population history of the Japanese oak Quercus mongolica var. crispula and related species revealed by chloroplast DNA variation. Genes & Genetic Systems, 82 (6), 465–477.

    Oku, T. (2003) Microlepidoptera of the Iwate Prefecture. Transactions of the Iwate Entomological Society, Supplement 2, 1–157. [in Japanese]

    Ohshima, I. (2013) In: Nasu, Y., Hirowatari, T., Sakamaki, Y. & Kishida, Y. (Eds.), The standard of moths in Japan IV. Gakken Education Publishing, Tokyo, pp. 10–13. [in Japanese]

    Owada, M., Arita, Y., Jinbo, U., Kishida, Y., Nakajima, H., Ikeda, M. & Hirano, N. (2006) Monitoring Survey (2000–2005) of Moths (Insecta, Lepidoptera) in the Garden of the Imperial Palace, Tokyo, Central Japan. Memoirs of the National Science Museum, 43, 37–136. [in Japanese]

    Puplesis, R.K. (1984) Novye vidy Molej-malyutok (Lepidoptera, Nepticulidae) iz juzhnogo Primor’ya. (New species of nepticulids (Lepidoptera, Nepticulidae) from southern Primorye). Entomologicheskoe Obozrenie, 63, 111–125. [in Russian]

    Puplesis, R.K. (1985) Novye vidy molej-malyutok (Lepidoptera, Nepticulidae) s yuga dal’nego vostoka i Tadzhikistana (New species of the nepticulid moths from Southern far east and Tadzhikistan). Trudy Zoologicheskogo Instituta Akademiya Nauk SSSR, 134, 59–72. [in Russian]

    Puplesis, R. (1988) New species of plant mining Lepidoptera (Nepticulidae, Tischeriidae) from central Asia. Stapfia, 16, 273–290. Available from: (Accessed 6 Dec. 2019)

    Puplesis, R. (1994) The Nepticulidae of eastern Europe and Asia. Western, central and eastern parts. Backhuys Publishers, Leiden, 290 pp.

    Rocienė, A. & Stonis, J.R. (2013) Nepticulidae (Lepidoptera) of East Asia (2). Study of a collection sample deposited at the Russian Academy of Sciences, with descriptions of new species and a checklist. Zootaxa, 3652 (2), 75–116.

    Ronquist, F., Teslenko, M., van der Mark, P., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) MrBayes 3.2: efficient bayesian phylogenetic inference and model choice across a large model space. Systematic Biology, 61, 539–542.

    Satake, Y., Hara, H., Watari, S. & Tominari, T. (1989) "Nihon no Yasei-shokubutsu Mokuhon I." Heibon-sha, Tokyo, 321 pp. [in Japanese]

    Stamatakis, A. (2014) RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies, Bioinformatics, 30, 1312–1313.

    Stonis, J.R. & Remeikis, A. (2018) Odd species of Nepticulidae (Lepidoptera) from the American rainforest and southern Andes. Zootaxa, 4392 (3), 458–468.

    Stonis, J.R. & Rocienė, A. (2013) Nepticulidae (Lepidoptera) of East Asia (1). Reexamination of the male genitalia of types deposited at the Russian Academy of Sciences. Zootaxa, 3652 (1), 1–59.

    Stonis, J.R. & Rocienë, A. (2014) Additions to the Nepticulidae (Lepidoptera) of East Asia, with descriptions of three new species from Primorskiy Kray. Zootaxa, 3846 (2), 204–220.

    Stonis, J.R., Navickaite, A. & Diškus, A. (2015) A puzzle regarding the Siberian Ectoedemia jacutica (Lepidoptera: Nepticulidae): re-examination and the first photographic documentation of the type series. Biologija, 61, 116–122.

    Stonis, J.R., Diškus, A., Remeikis, A. & Cumbicus, N. (2016) Rosaceae-feeding Nepticulidae (Lepidoptera) of South America: some taxonomic and trophic diversity revealed. Biologija, 62 (4), 215–232.

    Tanabe, A.S. (2011) Kakusan4 and Aminosan: two programs for comparing nonpartitioned, proportional, and separate models for combined molecular phylogenetic analyses of multilocus sequence data. Molecular Ecology Resources, 11, 914–921.

    van Nieukerken, E.J. (1985) A taxonomic revision of the western Palearctic species of the subgenera Zimmermannia Hering and Ectoedemia Busck s. str. (Lepidoptera, Nepticulidae), with notes on their phylogeny. Tijdschrift voor Entomologie, 128, 1–164. Available from: (Accessed 6 Dec. 2019)

    van Nieukerken, E.J. & Berggren, K. (2011) Ectoedemia rosae, a new species from the French Alps and Norway (Lepidoptera: Nepticulidae). Tijdschrift voor Entomologie, 154, 181–191.

    van Nieukerken, E.J., Laštůvka, A. & Laštůvka, Z. (2010) Western Palaearctic Ectoedemia (Zimmermannia) Hering and Ectoedemia Busck s. str. (Lepidoptera: Nepticulidae): five new species and new data on distribution, hostplants and recognition. ZooKeys, 32, 1–82.

    van Nieukerken, E.J., Doorenweerd, C., Stokvis, F.R. & Groenenberg, D.S.J. (2012) DNA barcoding of the leaf-mining moth subgenus Ectoedemia s. str. (Lepidoptera: Nepticulidae) with COI and EF1—α: two are better than one in recognising cryptic species. Contributions to Zoology, 81, 1–24. Available from: (Accessed 6 Dec. 2019)

    van Nieukerken, E.J., Doorenweerd, C., Hoare, R. & Davis, D. (2016) Revised classification and catalogue of global Nepticulidae and Opostegidae (Lepidoptera, Nepticuloidea). ZooKeys, 628, 65–246.

    van Nieukerken, E.J., Gilrein, D.O. & Eiseman, C.S. (2018) Stigmella multispicata Rociene. & Stonis, an Asian leafminer on Siberian elm, now widespread in eastern North America (Lepidoptera, Nepticulidae). ZooKeys, 784, 95–125.

    Wilkinson, C. & Newton, P.J. (1981) The micro-lepidopteran genus Ectoedemia Busck (Nepticulidae) in North America. Tijdschrift voor Entomologie, 124, 27–92. Available from: (Accessed 6 Dec. 2019)

    Yonekura, K. & Kajita, T. (2003) BG Plants wamei—gakumei index (YList). Available from: (accessed 4 March 2019) [in Japanese]

    Zhang, Z., Schwartz, S., Wagner, L. & Miller, W. (2000) A greedy algorithm for aligning DNA sequences. Journal of Computational Biology, 7 (1–2), 203–214.