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Type: Article
Published: 2019-12-16
Page range: 275–292
Abstract views: 212
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A new species of Zhangixalus (Anura: Rhacophoridae), previously confused with Zhangixalus smaragdinus (Blyth, 1852)

Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, Guilin 541004, China Guangxi Key Laboratory of Rare and Endangered Animal Ecology, College of Life Science, Guangxi Normal University, Guilin 541004, China
State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China
Institute of Continuing Education, Sichuan Normal University, Chengdu, Sichuan 610068, China
Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, Guilin 541004, China Guangxi Key Laboratory of Rare and Endangered Animal Ecology, College of Life Science, Guangxi Normal University, Guilin 541004, China
State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China
Amphibia Zhangixalus pachyproctus sp. nov. Yunnan Southeast Asia

Abstract

We describe a new species of Zhangixalus from southern Yunnan of China, Vietnam, and Thailand based on morphological and molecular evidence. The new species had been confused with Zhangixalus smaragdinus (Blyth, 1852) in the past. Zhangixalus pachyproctus sp. nov. can be distinguished from Z. smaragdinus morphologically by the protruding vent in adult males, large thick grey reticular mottles below the white stripe on flank, more oblique snout in profile and wider head, longer snout, greater internarial distance, larger tympanum and longer hindlimb. The new species can be distinguished from other species of Zhangixalus with green dorsum by the following combination of characters: body size larger (SVL of adult males: 74.2–83.3 mm; SVL of adult female: 102.4 mm); dorsum smooth; narrow white stripes along edge of the lower jaw, body sides, outer side of limbs and above the vent; absence of brown bands on canthus rostralis, upper eyelid and supratympanic fold; webbing between fingers and toes complete except between the first two fingers; and internal single subgular vocal sac. Phylogeny based on comparison of 16S rRNA sequences suggests that the new species is the sister taxon to Z. smaragdinus and the two species differ by 7.63% in the uncorrected pairwise distance of 16S sequences.

 

References

  1. Ahmed, M.F., Das, A. & Dutta, S.K. (2009) Amphibians and Reptiles of Northeast India. A Photographic Guide. Aaranyak, Guwahati, 169 pp.

    AmphibiaChina (2019) The database of Chinese amphibians. Kunming Institute of Zoology (CAS), Kunming, Yunnan, China. Available from: http://www.amphibiachina.org/ (accessed 20 June 2019)

    Anders, C. & Rai, K.R. (2002) Rhacophorus maximus Günther, 1858. In: Schleich, H.H. & Kästle, W. (Eds.), Amphibians and Reptiles of Nepal: Biology, Systematics, Field Guide. A.R.G. Gantner Verlag K.G., Ruggell, pp. 337–340.

    Anderson, J. (1871) On some Indian Reptiles. Proceedings of the Zoological Society of London, 1871, 149–211.

    Andersson, L.G. (1939) Batrachians from Burma collected by Dr. R. Malaise, and from Bolivia and Ecuador collected by Dr. C. Hammarlund. Arkiv för Zoologi, Stockholm, 30 (23), 1–24.

    Blanford, W.T. (1881) On a collection of reptiles and frogs chiefly from Singapore. Proceedings of the Zoological Society of London, 1881, 215–226.

    https://doi.org/10.5962/bhl.title.101596

    Blyth, E. (1852) Report of Curator, Zoological Department. Journal of the Asiatic Society of Bengal, 21, 341–358.

    Bordoloi, S., Bortamuli, T. & Ohler A. (2007) Systematics of the genus Rhacophorus (Amphibia, Anura): identity of red-webbed forms and description of a new species from Assam. Zootaxa, 1653 (1), 1–20.

    https://doi.org/10.11646/zootaxa.1653.1.1

    Boulenger, G.A. (1893) Concluding report on the reptiles and batrachians obtained in Burma by Signor L. Fea dealing with the collection made in Pegu and the Karin Hills in 1887–88. Annali del Museo Civico di Storia Naturale di Genova, Serie 2, 13, 304–347.

    Boulenger, G.A. (1895) Descriptions of four new batrachians discovered by Mr. Charles Hose in Borneo. Annals and Magazine of Natural History, Series 6, 16, 169–171.

    https://doi.org/10.1080/00222939508680249

    Chan, K.O. & Grismer, L.L. (2010) Re-assessment of the Reinwardt’s gliding frog, Rhacophorus reinwardtii (Schlegel 1840) (Anura: Rhacophoridae) in southern Thailand and Peninsular Malaysia and its re-description as a new species. Zootaxa, 2505 (1), 40–50.

    https://doi.org/10.11646/zootaxa.2505.1.2

    Chan-ard, T. (2003) A photographic Guide to Amphibians in Thailand. Darnsutha Press, Bangkok, 175 pp.

    Deepak, C.K., Payra, A., Tripathy, B. & Chandra, K. (2019) Observation on rapid physiological color change in Giant tree frog Rhacophorus smaragdinus (Blyth, 1852) from Namdapha Tiger Reserve, Arunachal Pradesh, India. Herpetozoa, 32, 95–99.

    https://doi.org/10.3897/herpetozoa.32.e36023

    Dehling, J.M. (2011) Taxonomic status of the population of Rhacophorus angulirostris Ahl, 1927 (Anura: Rhacophoridae) from Sumatera Barat (West Sumatra) and its description as a new species. Salamandra, 47, 133–143.

    Dehling, J.M. (2015) A new species of Rhacophorus (Anura: Rhacophoridae) from Gunung Kinabalu, Borneo. Salamandra, 51, 1–11.

    Fei, L. (1999) Atlas of Amphibians of China. Henan Publishing House of Science and Technol­ogy, Zhengzhou, 432 pp.

    Fei, L., Ye, C. & Jiang, J. (2010) Colored Atlas of Chinese Amphibians. Sichuan Publishing House of Science and Technology, Chengdu, 464 pp.

    Frost, D.R. (2019) Amphibian Species of the World: and Online Reference. Version 6.0. Ameri­can Museum of Natural History, New York. Available from: http://research.amnh.org/herpetology/amphibia/index.html/ (accessed 3 April 2019)

    Günther, A. (1858) Neue Batrachier in der Sammlung des britischen Museums. Archiv für Naturgeschichte, 24, 319–328.

    https://doi.org/10.5962/bhl.part.5288

    Günther, A. (1869) First account of species of tailless batrachians added to the collection of the British Museum. Proceedings of the Zoological Society of London, 1868, 478–490.

    Hallowell, E. (1861) Report upon the Reptilia of the North Pacific Exploring Expedition, under command of Capt. John Rogers, U.S. N. Proceedings of the Academy of Natural Sciences of Philadelphia, 12, 480–510.

    Hedges, S.B. (1994) Molecular evidence for the origin of birds. Proceedings of the National Academy of Sciences of the United States of America, 91, 2621–2624.

    https://doi.org/10.1073/pnas.91.7.2621

    Huelsenbeck, J.P. & Ronquist, F. (2001) MrBayes: Bayesian inference of phylogeny. Bioinformatics, 17, 754–755.

    https://doi.org/10.1093/bioinformatics/17.8.754

    Jerdon, T.C. (1870) Notes on Indian herpetology. Proceedings of the Asiatic Society of Bengal, 1870, 66–85.

    Jiang, D., Jiang, K., Ren, J., Wu, J. & Li, J. (2019) Resurrection of the genus Leptomantis, with description of a new genus to the family Rhacophoridae (Amphibia: Anura). Asian Herpetological Research, 10 (1), 1–12.

    https://doi.org/10.16373/j.cnki.ahr.180058

    Kocher, T.D., Thomas, W.K., Meyer, A., Edwards, S.V., Pääbo, S., Villablanca, F.X. & Wilson, A.C. (1989) Dynamics of mitochondrial DNA evolution in animals: amplification and sequencing with conserved primers. Proceedings of the National Academy of Sciences of the United States of America, 86, 6196–6200.

    https://doi.org/10.1073/pnas.86.16.6196

    Kuhl, H. & van Hasselt, J.C. (1822) Uittreksels uit brieven van de Heeren Kuhl en van Hasselt, aan de Heeren C. J. Temminck, Th. van Swinderen en W. de Haan. Algemeene Konst-en Letter-Bode, 7, 99–104.

    Kuramoto, M. & Wang, C. (1987) A new rhacophorid treefrog from Taiwan, with comparisons to Chirixalus eiffingeri (Anura, Rhacophoridae). Copeia, 1987, 931–942.

    https://doi.org/10.2307/1445556

    Lalramdinfeli, M.H. & Lalremsanga, H.T. (2017) Observation on breeding behavior and analysis of the advertisement calls of Rhacophorus maximus Günther, 1858 (Anura: Rhacophoridae). Science Vision, 16 (4), 156–165.

    Liu, C. & Hu, S. (1960) Preliminary report of Amphibia from southern Yunnan. Acta Zoologica Sinica, 11, 508–538.

    Liu, C. & Hu, S. (1962) A herpetological report of Kwangsi. Acta Zoologica Sinica, 14, 73–104.

    Luu, V.Q., Calame, T., Nguyen, T.Q., Ohler, A., Bonkowsk, M. & Ziegler, T. (2014) First records of Gracixalus supercornutus (Orlov, Ho and Nguyen, 2004) and Rhacophorus maximus Günther, 1858 from Laos. Herpetology Notes, 7, 419–423.

    Myers, C.W. & Duellman, W.E. (1982) A new species of Hyla from Cerro Colorado, and other tree frog records and geographical notes from western Panama. American Museum Novitates, 2752, 1–32.

    Nguyen, T.Q., Pham, C.T., Le, D.T., Nguyen, B.V. & Nguyen, S.LH. (2015) Diversity of tree frogs (Amphibia: Anura: Rhacophoridae) from Dien Bien Province. In: Proceedings of the sixth National Scientific Conference on Ecology and Biological Resources. Agriculture Publishing House, Hanoi, pp. 954–959.

    Ohler, A. & Delorme, M. (2006) Well known does not mean well studied: Morphological and molecular support for existence of sibling species in the Javanese gliding frog Rhacophorus reinwardtii (Amphibia, Anura). Comptes Rendus Biologies, 329, 86–97.

    https://doi.org/10.1016/j.crvi.2005.11.001

    Ohler, A. & Deuti, K. (2018) Polypedates smaragdinus Blyth, 1852—a senior subjective synonym of Rhacophorus maximus Günther, 1858. Zootaxa, 4375 (2), 273–280.

    https://doi.org/10.11646/zootaxa.4375.2.7

    Ohler, A., Marquis, O., Swan, S.R. & Grosjean, S. (2000) Amphibian biodiversity of Hoang Lien Nature Reserve (Lao Cai Province, northern Vietnam) with description of two new species. Herpetozoa, Wien, 13, 71–87.

    Pan, T., Zhang, Y., Wang, H., Wu, J., Kang, X., Qian, L., Li, K., Zhang, Y., Chen, J., Rao, D., Jiang, J. & Zhang, B. (2017) A new species of the genus Rhacophorus (Anura: Rhacophoridae) from Dabie Mountains in East China. Asian Herpetological Research, 8, 1–13.

    Peters, W. (1867) Herpetologische Notizen. Monatsberichte der Königlichen Preussische Akademie des Wissenschaften zu Berlin, 1867, 13–37.

    Peters, W. (1871) Über neue Reptilien aus Ostafrika und Sarawak (Borneo), vorzüglich aus der Sammlung des Hrn. Marquis J. Doria zu Genua. Monatsberichte der Königlich Preussischen Akademie der Wissenschaften zu Berlin, 1871, 566–581.

    Pham, A.V., Nguyen, T.Q., Ziegler, T. & Nguyen, T.T. (2017a) New records of tree frogs (Anura: Rhacophoridae: Rhacophorus) from Son La Province, Vietnam. Herpetology Notes, 10, 379–386.

    Pham, C.T., An, H.T., Herbst, S., Bonkowski, M., Ziegler, T. & Nguyen, T.Q. (2017b) First report on the amphibian fauna of Ha Lang karst forest, Cao Bang Province, Vietnam. Bonn zoological Bulletin, 66, 37–53.

    Pham, C.T., Nguyen, T.Q., Hoang, C.V. & Ziegler, T. (2016) New records and an updated list of amphibians from Xuan Lien Nature Reserve, Thanh Hoa Province, Vietnam. Herpetology Notes, 9, 31–41.

    Posada, D. & Crandall, K.A. (1998) Modeltest: testing the model of DNA substitution. Bioinfor­matics, 14, 817–818.

    https://doi.org/10.1093/bioinformatics/14.9.817

    Roux J. (1904) Reptilien und Amphibien aus Celebes. Verhandlungen der Naturforschenden Gesellschaft in Basel, 15, 425–433.

    Rowley, J.J.L., Tran, D.T.A., Hoang, H.D. & Le, D.T.T. (2012) A new species of large flying frog (Rhacophoridae: Rhacophorus) from lowland forests in Southern Vietnam. Journal of Herpetology, 46, 480–487.

    https://doi.org/10.1670/11-261

    Shah, K.B. & Tiwari, S. (2004) Herpetofauna of Nepal. A conservation Companion. IUCN Nepal, Kathmandu, 237 pp.

    Silvestro, D. & Michalak, I. (2012) raxmlGUI: a graphical front-end for RAxML. Organisms Di­veristy & Evolution, 12, 335–337.

    https://doi.org/10.1007/s13127-011-0056-0

    Streicher, J.W., Hamidy, A., Harvery, M.B., Anders, B., Shaney, K.J., Kurniawan, N. & Smith, E.N. (2014) Mitochondrial DNA reveals a new species of parachuting frog (Rhacophoridae: Rhacophorus) from Sumatra. Zootaxa, 3878 (4), 351–365.

    https://doi.org/10.11646/zootaxa.3878.4.2

    Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M. & Kumar, S. (2011) MEGA5: molecu­lar evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution, 28, 2731–2739.

    https://doi.org/10.1093/molbev/msr121

    Temminck, C.J. & Schlegel, H. (1838) Fauna Japonica auctore Ph. Fr. De Siebold. Reptilia. In: Fauna Japonica sive Descriptio animalium, quae in itinere per Japoniam, jussu et auspiciis superiorum, qui summum in India Batava imperium tenent, suscepto, annis 1823–1830 collegit, notis, observationibus et adumbrationibus illustravit Ph. Fr. de Siebold. Vol. 3. J. G. Lalau, Leiden, pp. i–xxi + 1–144.

    Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, J. & Higgins, D.G. (1997) The CLUSTAL X windows interface: flexible strategies for multiple sequence alignment aided by qual­ity analysis tools. Nucleic Acids Research, 25, 4876–4882.

    https://doi.org/10.1093/nar/25.24.4876

    Wildenhues, M.J., Gawor, A., Nguyen, T.Q., Nguyen, T.T., Schmitz, A. & Ziegler, T. (2010) First description of larval and juvenile stages of Rhacophorus maximus Günther, 1859 “1858” (Anura: Rhacophoridae) from Vietnam. Revue Suisse De Zoologie, 117, 679–696.

    https://doi.org/10.5962/bhl.part.118618

    Yang, D. & Rao, D. (2008) Amphibia and Reptilia of Yunnan. Yunnan Science and Technology Press, Kunming, 409 pp.

    Youjaroen, M., Chuaynkern, C., Chuaynkern, Y., Duengkae, P., Taksintum, W. & Phochayavanich, R. (2018) Distribution of Rhacophorus feae Boulenger, 1893 and Rhacophorus maximus Günther, 1858 (Anura: Rhacophoridae) in Thailand. Russian Journal of Herpetology, 25 (3), 165–171.

    https://doi.org/10.30906/1026-2296-2018-25-3-165-171