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Type: Article
Published: 2020-06-08
Page range: 266–280
Abstract views: 119
PDF downloaded: 6

Molecular and morphological characters of Helicotylenchus Steiner, 1945 species from Iran with a note on the identity of H. pseudorobustus (Steiner, 1914) Golden, 1956

Plant Protection Department, Shahid Bahonar University, Kerman, Iran
Green Biotechnologies Research Centre of Excellence, University of Limpopo, Private Bag X1106, Sovenga 0727, South Africa
National Collection of Nematodes, Biosystematics, Agricultural Research Council, Plant Health and Protection, (ARC-PHP), Private Bag X134, Queenswood 0121, South Africa
Green Biotechnologies Research Centre of Excellence, University of Limpopo, Private Bag X1106, Sovenga 0727, South Africa
Nematoda 28S rDNA hierarchical clustering morphology phylogeny spiral nematode


Helicotylenchus species were recovered from natural areas of three provinces (Mazandaran, Kerman and Guilan) in Iran, with morphological and molecular characteristics suggesting that they were Helicotylenchus minzi and Helicotylenchus pseudorobustus. A phylogenetic analysis of the two Helicotylenchus species using 28S rDNA placed H. pseudrobustus and H. minzi in a clade with 1.00 posterior probability. Results suggest that the Helicotylenchus population identified using molecular characteristics as H. pseudorobustus is a paraphyletic group, with hierarchical clustering analysis indicating a close relationship between the Iranian and the Chinese populations of H. pseudorobustus. In conclusion, further molecular studies within the genus Helicotylenchus are needed, especially for morphologically similar populations that have been recognized as separate species.



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