Abstract
A new species of the gekkonid genus Hemiphyllodactylus is described from limestone karst forest of Tuyen Quang Province, northern Vietnam based on morphological differences and molecular divergence. Hemiphyllodactylus nahangensis sp. nov. is distinguished from the remaining congeners by having the unique combination of the following characters: a bisexual taxon; SVL of adults 41.4–43.6 mm; dorsal scale rows 18–23; ventral scale rows 9–13; chin scales bordering mental and first infralabial distinctly enlarged; 22–24 pore-bearing femoral and precloacal scales, in a continuous row, absent in females; digital lamella formula 3-4-5-4 (forefoot) and 4-5-5-5 (hindfoot); cloacal spur single, present in both sexes; dark lateral head stripe indistinct; postsacral mark cream and bearing anteriorly projecting arms. In phylogenetic analyses, the new species is placed in a clade that includes H. dushanensis, H. hongkongensis, H. huishuiensis, H. ngocsonensis and H. zugi. In terms of pairwise genetic distance, the new species is at least 5.9%–6.6% divergent from other congeners based on a fragment of the mitochondrial gene ND2.
References
Agarwal, I., Bauer, A.M., Pal, S.N., Srikanthan, A.N. & Khandekar, A. (2020) Two more new Hemiphyllodactylus Bleeker, 1860 (Squamata: Gekkonidae) from Tamil Nadu, India. Zootaxa, 4729 (2), 249–265.
https://doi.org/10.11646/zootaxa.4729.2.6
Agarwal, I., Khandekar, A., Giri, V.B. & Ramakrishnan, U. (2019) The hills are alive with geckos! A radiation of a dozen species on sky islands across peninsular India (Squamata: Gekkonidae, Hemiphyllodactylus) with the description of three new species, Organisms Diversity & Evolution, 19 (2), 341–361.
https://doi.org/10.1007/s13127-019-00392-5
Bain, R.H. & Hurley, M.M. (2011) A biogeographic synthesis of the amphibians and reptiles of Indochina. Bulletin of the American Museum of Natural History, 360, 1–138.
Barbour, T. (1924) A Yunnan gecko. Occasional Papers of the Boston Society of Natural History, 5, 133–135.
Boulenger, G.A. (1903) Descriptions of new lizards in the collection of the British Museum. The Annals and Magazine of Natural History, Series 7, 12 (70), 429–435.
https://doi.org/10.1080/00222930308678877
Bourret, R. (2009) Les lézards de l’Indochine. Edition Chimaira, Frankfurt am Main, 624 pp.
Cobos, A.L., Grismer, L.L., Wood, P.L.Jr, Quah, E.S.H., Anuar, S. & Muin, M.A. (2016) Phylogenetic relationships of geckos of the Hemiphyllodactylus harterti group, a new species from Penang Island, Peninsular Malaysia, and a likely case of true cryptic speciation. Zootaxa, 4107 (3), 367–380.
https://doi.org/10.11646/zootaxa.4107.3.5
Darriba, D., Taboada, G.L., Doallo, R. & Posada, D. (2012) jModelTest 2: more models, new heuristics and parallel computing. Nature Methods, 9 (8), 772.
https://doi.org/10.1038/nmeth.2109
Eliades, S.J., Phimmachak, S., Sivongxay, N., Siler, C.D. & Stuart, B.L. (2019) Two new species of Hemiphyllodactylus (Reptilia: Gekkonidae) from Laos. Zootaxa, 4577 (1), 131–147.
https://doi.org/10.11646/zootaxa.4577.1.8
Hoang, D.T., Chernomor, O., von Haeseler, A., Bui, M.Q. & Le, V.S. (2018) UFBoots: Improving the ultrafast bootstrap approximation. Molecular Biology and Evolution, 35, 518–522.
https://doi.org/10.1093/molbev/msx281
Grismer, L.L., Riyanto, A., Iskandar, D.T. & McGuire, J.A. (2014a) A new species of Hemiphyllodactylus Bleeker, 1860 (Squamata: Gekkonidae) from Pulau Enggano, southwestern Sumatra, Indonesia. Zootaxa, 3821 (4), 485–495.
https://doi.org/10.11646/zootaxa.3821.4.7
Grismer, L.L., Wood, P.L. Jr., Anuar, S., Muin, M.A., Quah, E.S.H. & Mcguire, J.A. (2013) Integrative taxonomy uncovers high levels of cryptic species diversity in Hemiphyllodactylus Bleeker, 1860 (Squamata: Gekkonidae) and the description of a new species from Peninsular Malaysia. Zoological Journal of the Linnean Society, 169, 849–880.
https://doi.org/10.1111/zoj.12064
Grismer, L.L., Wood, P.L., Anuar, S., Quah, E.S.H., Muin, M.A., Onn, C.K., Sumarli, A.X. & Loredo, A.I. (2015) Repeated evolution of sympatric, palaeoendemic species in closely related, co-distributed lineages of Hemiphyllodactylus Bleeker, 1860 (Squamata: Gekkonidae) across a sky-island archipelago in Peninsular Malaysia. Zoological Journal of the Linnean Society, 174, 859–876.
https://doi.org/10.1111/zoj.12254
Grismer, L.L., Wood, P.L. Jr. & Cota, M. (2014b) A new species of Hemiphyllodactylus Bleeker, 1860 (Squamata: Gekkonidae) from northwestern Thailand. Zootaxa, 3760 (1), 67–78.
https://doi.org/10.11646/zootaxa.3760.1.4
Grismer, L.L., Wood, P.L. Jr., Quah, S.H.E., Thura, K.M., Oaks, J.R. & Lin, A. (2020) Four new Burmese species of Hemiphyllodactylus Bleeker (Squamata: Gekkonidae) from distantly related parapatric clades from the Shan Plateau and Salween Basin. Zootaxa, 4758 (1), 45–82.
https://doi.org/10.11646/zootaxa.4758.1.2
Grismer, L.L., Wood, P.L. Jr., Thura, K.M., Zin, T., Quah, S.H.E., Murdoch, L.M., Grismer, S.M., Li, A., Kyaw, H. & Lwin, N. (2018a) Phylogenetic taxonomy of Hemiphyllodactylus Bleeker, 1860 (Squamata: Gekkonidae) with descriptions of three new species from Myanmar. Journal of Natural History, 2017, 881–915.
https://doi.org/10.1080/00222933.2017.1367045
Grismer, L.L., Wood, P.L. Jr., Zug, G.R., Thura, M.K., Grismer, M.S., Murdoch, M.L., Quah, E.S.H. & Lin, A. (2018b) Two more new species of Hemiphyllodactylus Bleeker (Squamata: Gekkonidae) from the Shan Hills of eastern Myanmar (Burma). Zootaxa, 4483 (2), 295–316.
https://doi.org/10.11646/zootaxa.4483.2.4
Guo, W., Zhou, K., Yan, J. & Li, P. (2015) A new species of Hemiphyllodactylus Bleeker, 1860 (Squamata: Gekkonidae) from western Yunnan, China. Zootaxa, 3974 (3), 377–390.
Le, M., Raxworthy, C.J., McCord, W.P. & Mertz, L. (2006) A molecular phylogeny of tortoises (Testudines: Testudinidae) based on mitochondrial and nuclear genes. Molecular Phylogenetics and Evolution, 40, 517−531.
https://doi.org/10.1016/j.ympev.2006.03.003
Macey, J.R., Larson, A., Ananjeva, N.B., Fang, Z. & Papenfuss, T.J. (1997) Two novel gene orders and the role of light-strand replication in rearrangement of the vertebrate mitochondrial genome. Molecular Biology and Evolution, 14, 91–104.
https://doi.org/10.1093/oxfordjournals.molbev.a025706
Ngo, T.V., Grismer, L.L., Pham, T.H. & Wood, P.L. Jr. (2014) A new species of Hemiphyllodactylus Bleeker, 1860 (Squamata: Gekkonidae) from Ba Na-Nui Chua Nature Reserve, Central Vietnam. Zootaxa, 3760 (4), 539–552.
https://doi.org/10.11646/zootaxa.3760.4.3
Nguyen, T.Q., Botov, A., Le, M.D., Nophaseud, L., Zug, G., Bonkowski, M. & Ziegler, T. (2014) A new species of Hemiphyllodactylus (Reptilia: Gekkonidae) from northern Laos. Zootaxa, 3827 (1), 45–56.
https://doi.org/10.11646/zootaxa.3827.1.4
Nguyen, T.Q., Do, Q.H., Ngo, H.T. Pham, A.V., Pham, C.T., Le, M.D. & Ziegler, T. (2020) Two new species of Hemiphyllodactylus (Squamata: Gekkonidae) from Hoa Binh Province, Vietnam. Zootaxa, 4801 (3), 513–536.
https://doi.org/10.11646/zootaxa.4801.3.5
Nguyen, T.Q., Lehmann, T., Le, M.D., Duong, H.T., Bonkowski, M. & Ziegler, T. (2013) A new species of Hemiphyllodactylus (Reptilia: Gekkonidae) from northern Vietnam. Zootaxa, 3736 (1), 89–98.
https://doi.org/10.11646/zootaxa.3736.1.5
Ronquist, F., Teslenko, M., van der Mark, P., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology, 61, 539−542.
https://doi.org/10.1093/sysbio/sys029
Smith, M.A. (1935) The Fauna of British India, including Ceylon and Burma. Reptiles and Amphibia. Vol. II. Sauria. Taylor and Francis, London, 440 pp.
Sukprasert, A., Sutthiwises, S., Lauhachinda, V. & Taksintum,W. (2018) Two new species of Hemiphyllodactylus Bleeker (Squamata: Gekkonidae) from Thailand. Zootaxa, 4369 (3), 363–376.
https://doi.org/10.11646/zootaxa.4369.3.4
Swofford, D.L. (2001) PAUP*. Phylogenetic Analysis Using Parsimony (* and Other Methods). Version 4. Sinauer Associates, Sunderland, Massachusetts. [program]
Taylor, E.H. (1963) The lizards of Thailand. The University of Kansas Science Bulletin, 44, 687–1077.
Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F. & Higgins, D.G. (1997) The ClustalX windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research, 25, 4876−4882.
https://doi.org/10.1093/nar/25.24.4876
Uetz, P., Hosek, J. & Hallermann, J. (Eds.) (2020) The Reptile Database. Available from: http://www.reptile-database.org (accessed 17 March 2020)
Yan, J., Lin, Y., Guo, W., Li, P. & Zhou, K. (2016) A new species of Hemiphyllodactylus Bleeker, 1860 (Squamata: Gekkonidae) from Guizhou, China. Zootaxa, 4117 (4), 543–554.
https://doi.org/10.11646/zootaxa.4117.4.6
Zhou, K.-Y., Liu, Y.-Z. & Yang, G.-P. (1981) Three new subspecies of Hemiphyllodactylus yunnanensis (Boulenger) from China. Acta Zootaxonomica Sinica, 6, 202–209. [in Chinese, English translation by H. Ota (1996) in Smithsonian Herpetological Information Service, 110, 8 pp., 1 pl.]
Zug, G.R. (2010) Speciation and dispersal in a low diversity taxon: The Slender Geckos Hemiphyllodactylus (Reptilia, Gekkonidae). Smithsonian Contributions to Zoology, 631, 1–70.