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Type: Article
Published: 2021-05-20
Page range: 333–360
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A description of the second species of the genus Bleptus Eaton, 1885 (Ephemeroptera: Heptageniidae) from Japan, and phylogenetic relationships of two Bleptus mayflies inferred from mitochondrial and nuclear gene sequences

Department of Biology, Faculty of Science, Shinshu University, Asahi 3-1-1, Matsumoto, Nagano 390-8621, Japan Department of Environmental System Science, Graduate School of Science and Technology, Shinshu University, Asahi 3-1-1, Matsumoto, Nagano 390-8621, Japan. Institute of Mountain Science, Shinshu University, Asahi 3-1-1, Matsumoto, Nagano 390-8621, Japan.
Department of Environmental System Science, Graduate School of Science and Technology, Shinshu University, Asahi 3-1-1, Matsumoto, Nagano 390-8621, Japan.
Department of Environmental System Science, Graduate School of Science and Technology, Shinshu University, Asahi 3-1-1, Matsumoto, Nagano 390-8621, Japan.
Department of Biology, Faculty of Science, Shinshu University, Asahi 3-1-1, Matsumoto, Nagano 390-8621, Japan
Institute of Mountain Science, Shinshu University, Asahi 3-1-1, Matsumoto, Nagano 390-8621, Japan.
Ephemeroptera aquatic insects Bayesian analysis geohistory phylogeography taxonomy

Abstract

A new mayfly species, Bleptus michinokuensis sp. nov. (Ephemeroptera: Heptageniidae) is described on the basis of specimens of male and female adults and mature nymphs collected at a seepage zone of a small freshwater branch of the ‘Tachiya-zawa-gawa’ River located amongst the northern foothills of Mt. Gassan (Shonai-machi Town, Yamagata Prefecture, Japan). This new Bleptus species is characterized by its clear fore and hind wings. That is, they neither exhibit the distinct black band on the fore wings, nor the characteristic darkened margins along the edges of both the fore and hind wings. Rather it has a blackish colored terminal half of its fore legs (i.e., tibial, tarsal and pretarsal segments). These features differ clearly when comparing them to the other known species, Bleptus fasciatus Eaton. The information and data describing the habitat and distribution range of this new species are also noted. We also examined and discussed the genetic relationship of two Bleptus mayflies to settle the taxonomic status, inferred from the partially sequenced cytochrome c oxidase subunit I (COI) and large mitochondrial ribosomal subunit (16S rRNA) genes, and also the nuclear internal transcribed spacer 2 (ITS2) gene sequences. Consequently, phenetic and molecular phylogenetic analyses agreed well in terms of clustering.

 

References

  1. Bae, Y.J., Yoon, I.B. & Chun, D.J. (1994) A catalogue of the Ephemeroptera of Korea. Entomological Research Bulletin, 20, 31−51.

    De Haan, W. (1841) Crustacea. In: von Siebold, P.F. (Ed.), 1883−1850, Fauna Japonica sive descriptio animalium, quate in itinere per Japoniam, jussu et auspiciis superiorum, qui summum in India Batava Imperium tenent, suscepto, annis 1823–1830 collegit, nois, observationibus et adumbrationibus illustravit (Crustacea). Lugundi-Batavorum, Leiden, pp. 243.

    Drummond, A.J, Suchard, M.A., Xie, D. & Rambaut, A. (2012) Bayesian phylogenetics with BEAUti and the BEAST 1.7. Molecular Biology and Evolution, 29, 69−1973.

    https://doi.org/10.1093/molbev/mss075

    Eaton, A.E. (1885) A revisional monograph of recent Ephemeridae or mayflies. Part 4. The Transactions of the Linnean Society of London, 1885, 229−281.

    https://doi.org/10.1111/j.1096-3642.1885.tb01550d.x

    Felsenstein, J. (1981) Evolutionary trees from DNA sequences: a maximum likelihood approach. Journal of Molecular Evolution, 17, 368−376.

    https://doi.org/10.1007/BF01734359

    Felsenstein, J. (1985) Confidence limits on phylogenies: An approach using the bootstrap. Evolution, 39, 783−791.

    https://doi.org/10.1111/j.1558-5646.1985.tb00420.x

    Fitch, W.M. (1971) Towards defining the course of evolution: minimum change for a specific tree topology. Systematic Zoology, 20, 406−416.

    https://doi.org/10.1093/sysbio/20.4.406

    Folmer, O., Black, M., Hoeh, W., Lutz R. & Vrijenhoek, R. (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3, 294–299. [https://www.mbari.org/wp-content/uploads/2016/01/Folmer_94MMBB.pdf]

    Gose, K. (1985) Ephemeroptera. In: Kawai, T. (Ed.), An Illustrated Book of Aquatic Insects of Japan. 1st Edition. Tokai University Press, Tokyo, pp. 7−32.

    Hall, B.G. (2001) Phylogenetic trees made easy: a how-to manual for molecular biologists. Available from: http://paup.phylosolutions.com (accessed 19 April 2021)

    Huelsenbeck, J.P. & Ronquist, F. (2001) MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics, 17, 754−755. [http://146.6.100.192/users/CH391L/Handouts/MRBAYES.pdf]

    https://doi.org/10.1093/bioinformatics/17.8.754

    Imanishi, K. (1934) Mayflies from Japanese torrents. IV. Notes on the genus Epeorus. Annotationes Zoologicae Japoneses, 14, 381−395.

    https://ci.nii.ac.jp/naid/10015544711/

    Ishiwata, S. (2001) A checklist of Japanese Ephemeroptera. Proceedings of the 1st Symposium of the Aquatic Entomological Society of East Asia, 1, 55−84.

    Jordan, D.S. & Snyder, J.O. (1901) List of fishes collected in 1883 and 1885 by Pierre Louis Jouy and preserved in the United States National Museum, with descriptions of six new species. Proceedings of the United States National Museum, 23, 739−769. [https://repository.si.edu/bitstream/handle/10088/13601/1/USNMP-23_1235_1901.pdf]

    https://doi.org/10.5479/si.00963801.23-1235.739

    Kato, Y., Mori, Y. & Tojo, K. (2013) Molecular phylogeographic analysis of East Asian cryptoperlan stoneflies (Insecta: Plecoptera, Peltoperlidae). Limnology, 14, 179−194.

    https://doi.org/10.1007/s10201-012-0395-3

    Kitagawa, T., Watanabe, M., Kitagawa, E., Yoshioka, M., Kashiwagi, M. & Okazaki, T. (2003) Phylogeography and the maternal origin of the tetraploid form of the Japanese spined loach, Cobitis biwae, revealed by mitochondrial DNA analysis. Ichthyological Research, 50, 318−325.

    https://doi.org/10.1007/s10228-003-0174-6

    Koizumi, I., Usio, N., Kawai, T., Azuma, N. & Masuda, R. (2012) Loss of genetic diversity means loss of geological information: The endangered Japanese crayfish exhibits remarkable historical footprints. PLoS ONE, 7, e33986.

    https://doi.org/10.1371/journal.pone.0033986

    Maddison, D.R., Swofford, D.J. & Maddison, W.P. (1997) NEXUS: an extensible file format for systematic information. Systematic Biology, 46, 590−621.

    https://doi.org/10.1093/sysbio/46.4.590

    Matsumura, S. (1931) 6000 Illustrated Insects of the Japanese Empire. Toko Shoin, Tokyo, 1689 pp.

    Minato, M. (1974) Quaternary of Japan. Tokyo. Tsukiji-Shokan, Tokyo, 167 pp.

    Miyairi, K. & Tojo, K. (2007a) Biology of the mayfly Bleptus fasciatus Eaton (Insecta: Ephemeroptera, Heptageniidae), with special reerence to the distribution, habitat environment, life cycle, and nuptial behavior. Limnology, 8, 85−93.

    https://doi.org/10.1007/s10201-007-0203-7

    Miyairi, K. & Tojo, K. (2007b) Notes of the mayfly Bleptus fasciatus Eaton (Insecta: Ephemeroptera, Heptageniidae), with reference to the type locality (Tabuhara, Kiso, Nagano Prefecture), distribution and biology. Bulletin of Shiojiri City Museum Naturak History, 9, 46−51. [in Japanese]

    Needham, J.G. & Betten, C. (1901) Aquatic insects in the Adirondacks. Bulletin of New York State Museum, 47, 383−612.

    Ogitani, M., Sekiné, K. & Tojo, K. (2011) Habitat segregation and genetic relationship of two heptageniid mayflies, Epeorus latifolium and Epeorus l-nigrus, in the Shinano-gawa River basin. Limnology, 12, 117−125.

    https://doi.org/10.1007/s10201-010-0328-y

    Otofuji, Y. (1996) Large tectonic movement of the Japan Arc in late Cenozoic times inferred from paleomagnetism: review and synthesis. Island Arc, 5, 229−249.

    https://doi.org/10.1111/j.1440-1738.1996.tb00029.x

    Otofuji, Y., Matsuda, T. & Nohda, S. (1985) Opening mode of the Japan Sea inferred from paleomagnetism of the Japan arc. Nature, 317, 603−604.

    https://doi.org/10.1038/317603a0

    Otofuji, Y., Itaya, T. & Matsuda, T. (1991) Rapid rotation of Southwest Japan-paleomagnetism and K-Ar ages of Miocenevolcanic rocks of Southwest Japan. Geophysical Journal International, 105, 397−405.

    https://doi.org/10.1111/j.1365-246X.1991.tb06721.x

    Papadopoulou, A., Anastasiou, L. & Vogler, P.A. (2010) Revisiting the insect mitochondrial molecular clock: The Mid-Aegean Trench calibration. Molecular Biology and Evolution, 27, 1659−1672.

    https://doi.org/10.1093/molbev/msq051

    Poyarkov, N.A. Jr, Che, J., Min, M.S., Yan, F., Li, C., Iizuka, K. & Vieites, D.R. (2012) Review of the systematics, morphology and distribution of Asian clawed Salamanders, genus Onychodactylus (Amphibia, Caudata: Hynobiidae), with the description of four new species. Zootaxa, 3465 (1), 1−106.

    https://doi.org/10.11646/zootaxa.3465.1.1

    Rambaut, A. (2009) FigTree version 1.3.1. Available from: http://tree.bio.ed.ac.uk/software/figtree/ (accessed 19 Apirl 2021)

    Rambaut, A. & Drummond, A.J. (2007) Tracer. Version 1.5. MCMC Trace analysis tool. Available from: http://tree.bio.ed.ac.uk/software/tracer/ (accessed 19 April 2021)

    Saitou, N. & Nei, M. (1987) The neighbor-joining method: A new method for reconstructing phylogenetic trees. Molecular Biology and Evolution, 4, 406−425.

    https://doi.org/10.1093/oxfordjournals.molbev.a040454

    Schwarz, G. (1978) Estimating the dimension of a model. The Annals of Statistics, 6, 461−464. [https://projecteuclid.org/euclid.aos/1176344136]

    https://doi.org/10.1214/aos/1176344136

    Stamatakis, A. (2006) RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics Applications Note, 22, 2688−2690.

    https://doi.org/10.1093/bioinformatics/btl446

    Strahler, A.N. (1957) Quantitative analysis of watershed geomorphology. Transactions American Geophysical Union, 38, 913−920.

    https://doi.org/10.1029/TR038i006p00913

    Suzuki, K., Watanabe, Y. & Tojo, K. (2020) Embryogenesis of the damselfly Euphaea yayeyamana Oguma (Insecta: Odonata: Euphaeidae), with special reference to the formation of their larval abdominal ‘gill-like’ appendages. Entomological Science, 23, 280−293.

    https://doi.org/10.1111/ens.12421

    Takenaka, M. & Tojo, K. (2019) The ancient origin and large genetic differentiation across the geotectonic faults of a dipteromimid mayfly family endemic to the Japanese Islands. Biological Journal of the Linnean Society, 126, 555−573.

    https://doi.org/10.1093/biolinnean/bly192

    Takenaka, M., Tokiwa, T. & Tojo, K. (2019) Concordance between molecular biogeography of Dipteromimus tipuliformis and geological history in the local fine scale (Ephemeroptera, Dipteromimidae). Molecular Phylogenetics & Evolution, 139, 106547.

    https://doi.org/10.1016/j.ympev.2019.106547

    Tamura, K., Stecher, G., Peterson, D., Filipski, A. & Kumar, S. (2013) MEGA6: Molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution, 30, 2725−2729.

    https://doi.org/10.1093/molbev/mst197

    Tanabe, A.S. (2007) KAKUSAN: A computer program to automate the selection of a nucleotide substitution model and the configuration of a mixed model on multilocus data. Molecular Ecology Notes, 7, 962−964.

    https://doi.org/10.1111/j.1471-8286.2007.01807.x

    Tanabe, A.S. (2008) MrBayes5D. Available from: http://www.fifthdimension.jp/ (accessed 19 April 2021)

    Thompson, J.D., Higgins, D.G. & Gibson, T.J. (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research, 22, 4673−4680.

    https://doi.org/10.1093/nar/22.22.4673

    Tojo, K. (2005) Genetic diversity in a headwater-specific aquatic insect Dipteromimus tipuliformis McLachlan (Ephemeroptera, Dipteromimidae), based on the mitochondrial 16S rRNA gene sequences. Ecology and Civil Engineering, 7, 119−127. [in Japanese with English abstract]

    Tojo, K. (2008) Genetic diversity in headwater-specific mayflies based on the mitochondrial 16S rRNA gene sequences. In: Hauer, F.R., Stanford, A. & Newell, R.L. (Eds.), International Advances in the Ecology, Zoogeography and Systematics of Mayflies and Stoneflies. University of California Press, Berkeley, Los Angeles, California, pp. 117−123. [https://escholarship.org/content/qt2cd0m6cp/qt2cd0m6cp.pdf#page=128]

    https://doi.org/10.1525/california/9780520098688.003.0009

    Tojo, K. (2010) Chapter 7. The current distribution of aquatic insects inhabiting river systems, with respect to the population and genetic structures. In: Harris, E.L. & Davies, N.E. (Ed.), Insect Habitats: Characteristics, Diversity and Management. Nova Science Publishers, Inc., New York, pp. 157−161.

    Tojo, K. & Machida, R. (2003) Techniques in embryological studies of mayflies (Insecta: Epehemeroptera). In: Gaino, E. (Ed.), Research Update on Ephemeroptera & Plecoptera. University of Perugia Press, Perugia, pp. 205−209. [https://www.researchgate.net/profile/Koji_Tojo/publication/228470216_Techniques_in_embryological_studies_of_mayflies_Insecta_Ephemeroptera/links/0046352b8cc7119542000000/Techniques-in-embryological-studies-of-mayflies-Insecta-Ephemeroptera.pdf]

    Tojo, K. & Matsukawa, K. (2003) A description of the second species of the family Dipteromimidae (Insecta, Ephemeroptera), and genetic relationship of two dipteromimid mayflies inferred from mitochondrial 16S rRNA gene sequences. Zoological Science, 20, 1249−1259.

    https://doi.org/10.2108/zsj.20.1249

    Tojo, K., Tanaka, Y., Kuranishi, R.B. & Kanada, S. (2010) Reproductive biology and adaptability of the invasive alien freshwater amphipod Crangonyx floridanus (Crustacea: Amphipoda, Crangonyctidae). Zoological Science, 27, 522−527.

    https://doi.org/10.2108/zsj.27.522

    Tojo, K., Sekiné, K., Takenaka, M., Isaka, Y., Komaki, S., Suzuki, T. & Schoville, S.D. (2017) Species diversity of insects in Japan: Their origins and diversification processes. Entomological Science, 20, 357−381.

    https://doi.org/10.1111/ens.12261

    Uéno M. (1928) Some Japanese mayfly nymphs. Memoirs of the College of Science, Kyoto Imperial University, Series B, 4 (1), 19–63, pls. 3–17. [http://www.ephemeroptera-galactica.com/pubs/pub_u/pubuenom1928p19.pdf]

    White, T., Bruns, T., Lee, S. & Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M.A., Gelfand, D.H., Sninsky, J.J. & White, T.J. (Eds.), PCR Protocols. Academic Press, San Diego, pp. 315–322.

    https://doi.org/10.1016/B978-0-12-372180-8.50042-1

    Yoshikawa, N. & Matsui, M. (2013) A new salamander of the genus Onychodactylus from Tsukuba Mountains, Eastern Honshu, Japan (Amphibia, Caudata, Hynobiidae). Current Herpetology, 32, 9−25.

    https://doi.org/10.5358/hsj.32.9