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Article
Published: 2021-08-06

A new species of Leptobrachella (Anura, Megophryidae) from Mount Pu Ta Leng, northwest Vietnam

EDGE of Existence Programme, Zoological Society of London, Regent’s Park, London, UK. Asian Turtle Program of Indo-Myanmar Conservation 1806, CT1–C14 Bac Ha Building, To Huu Street, Nam Tu Liem District, Hanoi, Vietnam.
Zoological Society of London, Regent’s Park, London, UK.
Hoang Lien National Park, Sa Pa, Lao Cai, Vietnam.
Hoang Lien National Park, Sa Pa, Lao Cai, Vietnam.
Australian Museum Research Institute, Australian Museum, 1 William St, Sydney, NSW, 2010, Australia. 6Centre for Ecosystem Science, School of Biological, Earth and Environmental Sciences, University of New South Wales, Sydney NSW 2052, Australia.
Amphibian Frog Hoang Lien Leptobrachiinae Leptolalax

Abstract

The Hoang Lien Range in northwest Vietnam is known to harbour five species of Leptobrachella, a specious genus of terrestrial frogs. We collected specimens of Leptobrachella from Mount Pu Ta Leng on the second highest peak in the Hoang Lien Range and use morphological and molecular data to show that this population represents a previously undescribed species which we name Leptobrachella graminicola sp. nov. after the abundance of calling males on sedge-like plants. This new species is closely related to L. bourreti but the new species is distinguished from L. bourreti and other congeneric species by a combination of the following characters: (1) a body size range of 23.1–24.6 mm in six adult males and 28.6–32.9 mm in five adult females; (2) skin on dorsum smooth, with many tubercles and lacking dermal ridges; (3) toes with rudimentary webbing and broad lateral fringes; (4) belly white with brown spots; (5) throat dark brown with light grey-brown flecks and spots; (6) a row of large white spots on the outer edge of the tarsus extending from the heel to the inner metatarsal tubercle, sometimes forming a long white stripe; and (7) a bicoloured iris. The new, high-elevation species is likely to be range-restricted and threatened by habitat loss and the activities of tourists.

 

References

  1. Altschul, S.F., Gish, W., Miller, W., Myers, E.W. & Lipman, D.J. (1990) Basic local alignment search tool. Journal of Molecular Biology, 215, 403–410. https://doi.org/10.1016/S0022-2836(05)80360-2
    Anderson, J. (1871) A list of the reptilian accession to the Indian Museum, Calcutta, from 1865 to 1870, with a description of some new species. Journal of the Asiatic Society of Bengal, 40 (2), 12–39.
    Bain, R.H., Nguyen, T.Q. & Doan, V.K. (2007) New herpetofaunal records from Vietnam. Herpetological Review, 38 (1), 107–117.
    Benson, D.A., Cavanaugh, M., Clark, K., Karsch-Mizrachi, I., Lipman, D.J., Ostell, J. & Sayers, E.W. (2017) GenBank. Nucleic Acids Research, 45 (database issue), D37–D42. https://doi.org/10.1093/nar/gkw1070
    Boulenger, G.A. (1893) Concluding report on the reptiles and batrachians obtained in Burma by Signor L. Fea dealing with the collection made in Pegu and the Karin Hills in 1887–88. Annali del Museo Civico di Storia Naturale di Genova, Serie 2, 13, 304–347.
    Bourret, R. (1937) Notes herpétologiques sur l’Indochine française. XIV. Les batraciens de la collection du Laboratoire des Sciences Naturelles de l’Université. Descriptions de quinze espèces ou variétés nouvelles. Annexe du Bulletin de l’Instruction Publique Hanoi, 1937, 5–56. [in French]
    Bourret, R. (1942) Les Batraciens de l’Indochine. Institut Océanographique de l’Indochine, Hanoi, 547 pp. [in French]
    Chen, J.M., Poyarkov, N.A., Suwannapoom, C., Lathrop, A., Wu, Y.H., Zhou, W.W., Yuan, Z.Y., Jin, J.Q., Chen, H.M., Liu, H.Q., Nguyen, T.Q., Nguyen, S.N., Duong, T.V., Eto, K., Nishikawa, K., Matsui, M., Orlov, N.L., Stuart, B.L., Brown, R.M., Rowley, J.J.L., Murphy, R.W., Wang, Y.Y. & Che, J. (2018) Large-scale phylogenetic analyses provide insights into unrecognized diversity and historical biogeography of Asian leaf-litter frogs, genus Leptolalax (Anura: Megophryidae). Molecular Phylogenetics and Evolution, 124, 162–171. https://doi.org/10.1016/j.ympev.2018.02.020
    Chen, J.M., Xu, K., Poyarkov, N.A., Wang, K., Yuan, Z.Y., Hou, M., Suwannapoom, C., Wang, J. & Che, J. (2020) How little is known about “the little brown frogs”: description of three new species of the genus Leptobrachella (Anura: Megophryidae) from Yunnan Province, China. Zoological Research, 41 (3), 292–313. https://doi.org/10.24272/j.issn.2095-8137.2020.036
    Chen, W.C., Liao, X., Zhou, S.C. & Mo, Y.M. (2019) A new species of Leptobrachella (Anura: Megophryidae) from southern Guangxi, China. Zootaxa, 4563 (1), 67–82. https://doi.org/10.11646/zootaxa.4563.1.3
    Chen, J.M., Zhou, W.W., Poyarkov, N.A., Stuart, B.L., Brown, R.M., Lathrop, A., Wang, Y.Y., Yuan, Z.Y., Jiang, K., Hou, M., Chen, H.M., Suwannapoom, C., Nguyen, S.N., Duong, T. Van, Papenfuss, T.J., Murphy, R.W., Zhang, Y.P. & Che, J. (2017) A novel multilocus phylogenetic estimation reveals unrecognized diversity in Asian horned toads, genus Megophrys sensu lato (Anura: Megophryidae). Molecular Phylogenetics and Evolution, 106, 28–43. https://doi.org/10.1016/j.ympev.2016.09.004
    Cheng, Y.L., Shi, S.C., Li, J., Liu, J., Li, S.Z. & Wang, B. (2021) A new species of the Asian leaf litter toad genus Leptobrachella Smith, 1925 (Anura, Megophryidae) from northwest Guizhou Province, China. ZooKeys, 1021, 81–107. https://doi.org/10.3897/zookeys.1021.60729
    Das, I., Tron, R.K.L., Rangad, D. & Hooroo, R.N. (2010) A new species of Leptolalax (Anura: Megophryidae) from the sacred groves of Mawphlang, Meghalaya, north-eastern India. Zootaxa, 2339 (1), 44–56. https://doi.org/10.11646/zootaxa.2339.1.2
    Delorme, M., Dubois, A., Grosjean, S. & Ohler, A. (2006) Une nouvelle ergotaxinomie des Megophryidae (Amphibia, Anura). Alytes, 24, 6–21. [in French]
    Dubois, A. (1983) Note préliminaire sur le genre Leptolalax Dubois, 1980 (amphibiens, anoures), avec diagnose d’une espèce nouvelle du Vietnam. Alytes, 2, 147–153. [in French]
    Duong, T.V., Do, D.T., Ngo, C.D., Nguyen, T.Q. & Poyarkov, N.A. (2018) A new species of the genus Leptolalax (Anura: Megophryidae) from southern Vietnam. Zoological research, 39, 185–201.
    https://doi.org/10.24272/j.issn.2095-8137.2018.009
    Eto, K., Matsui, M. & Nishikawa, K. (2015) Description of a new species of the genus Leptobrachella (Amphibia, Anura, Megophryidae) from Borneo. Current Herpetology, 34 (2), 128–139. https://doi.org/10.5358/hsj.34.128
    Eto, K., Matsui, M. & Nishikawa, K. (2016) A new highland species of dwarf litter frog genus Leptobrachella (Amphibia: Anura: Megophryidae) from Sarawak. Raffles Bulletin of Zoology, 64, 194–203.
    Fei, L., Ye, C.Y. & Huang, Y.Z. (1990) Key to Chinese Amphibians. Publishing House for Scientific and Technological Literature, Chongqing, 364 pp. [in Chinese]
    Fei, L., Ye, C.Y. & Jiang, J.P. (2012) Colored Atlas of Chinese Amphibians and their Distributions. Sichuan Publishing House of Science & Technology. Chengdu, 620 pp. [in Chinese]
    Felsenstein, J. (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution, 39, 783–791.
    https://doi.org/10.1111/j.1558-5646.1985.tb00420.x
    Frost, D.R. (2021) Amphibian Species of the World: an online reference. Version 6.1. Available from: http://research.amnh.org/ herpetologyamphibia/index.html (accessed 7 July 2021)
    Frost, D.R., Grant, T., Faivovich, J., Bain, R.H., Haas, A., Haddad, C.F., De Sa, R.O., Channing, A., Wilkinson, M., Donnellan, S.C. & Raxworthy, C.J. (2006) The amphibian tree of life. Bulletin of the American Museum of natural History, 2006 (297), 1–291. https://doi.org/10.1206/0003-0090(2006)297[0001:TATOL]2.0.CO;2
    Grosjean, S., Ohler, A., Chuaynkern, Y., Cruaud, C. & Hassanin, A. (2015) Improving biodiversity assessment of anuran amphibians using DNA barcoding of tadpoles. Case studies from Southeast Asia. Comptes rendus biologies, 338 (5), 351–361. https://doi.org/10.1016/j.crvi.2015.03.015
    Hall, T.A. (1999) BioEdit: A User-Friendly Biological Sequence Alignment Editor and Analysis Program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41, 95–98.
    Hedges, S.B. (1994) Molecular evidence for the origin of birds. Proceedings of the National Academy of Sciences of the United States of America, 91, 2621–2624. https://doi.org/10.1073/pnas.91.7.2621
    Hedges, S.B. & Maxson, L.R. (1993) A molecular perspective on lissamphibian phylogeny. Herpetolgical Monographs, 7, 27–42. https://doi.org/10.2307/1466949
    Hoang, C.V., Pahm, C.T. & Nguyen, T.T. (2016) Species composition of amphibians from Bat Xat nature reserve Lao Cai Province Vietnam. Proceedings of the 1st National Scientific Conference on Amphibians and Reptiles in Vietnam, 2016, 41–46. [in Vietnamese]
    Hoang, C.V., Nguyen, T.T., Luu, Q.V., Nguyen T.Q. & Jiang, J. (2019) A new species of Leptobrachella Smith 1925 (Anura: Megophryidae) from Thanh Hoa Province, Vietnam. Raffles Bulletin of Zoology, 67, 536–556
    Hoang, D.T., Chernomor, O., Von Haeseler, A., Minh, B.Q. & Vinh, L.S. (2018) UFBoot2: improving the ultrafast bootstrap approximation. Molecular Biology Evolution, 35 (2), 518−522. https://doi.org/10.1093/molbev/msx281
    Hou, Y., Zhang, M.E., Hu, F., Li, S., Shi, S., Chen, J., Mo, X.I. & Wang, B. (2018) A new species of the genus Leptolalax (Anura, Megophryidae) from Hunan, China. Zootaxa, 4444 (3), 247–266. https://doi.org/10.11646/zootaxa.4444.3.2
    Huelsenbeck, J.P., Ronquist, F., Nielsen, R. & Bollback, J.P. (2001) Bayesian inference of phylogeny and its impact on evolutionary biology. Science, 294, 2310–2314. https://doi.org/10.1126/science.1065889
    Inger, R.F., Orlov, N. & Darevsky, I. (1999) Frogs of Vietnam: a report on new collections. Fieldiana Zoology, 92, 1–46.
    IUCN (2012) IUCN Red List Categories and Criteria: Version 3.1. 2nd Edition. IUCN, Gland and Cambridge, iv + 32pp.
    IUCN SSC (2015) Leptolalax botsfordi. The IUCN Red List of Threatened Species 2015: e.T73727195A73727483. Available from: https://doi.org/10.2305/IUCN.UK.2015-4.RLTS.T73727195A73727483.en (accessed 20 February 2021)
    Jiang, K., Yan, F., Suwannapoom, C., Chomdej, S. & Che, J. (2013) A new species of the genus Leptolalax (Anura: Megophryidae) from northern Thailand. Asian Herpetological Research, 4 (2), 100–108. https://doi.org/10.3724/SP.J.1245.2013.00100
    Katoh, K., Rozewicki, J. & Yamada, K.D. (2019) MAFFT online service: multiple sequence alignment, interactive sequence choice and visualization. Briefings in bioinformatics, 20 (4), 1160–1166. https://doi.org/10.1093/bib/bbx108
    Khuc, V.Q., Tran, B.Q., Meyfroidt, P. & Paschke, M.W. (2018) Drivers of deforestation and forest degradation in Vietnam: An exploratory analysis at the national level. Forest Policy and Economics, 90, 128–141. https://doi.org/10.1016/j.forpol.2018.02.004
    Kumar, S. Stecher, G. & Tamura, K. (2016) MEGA7: molecular Evolutionary Genetics Analysis version 7.0for bigger datasets. Molecular Biology and Evolution, 33, 1870–1874. https://doi.org/10.1093/molbev/msw054
    Kropachev, I.I., Orlov, N.L., Ninh, H.T. & Nguyen, T.T. (2019) A new species of Rhacophorus genus (Amphibia: Anura: Rhacophoridae: Rhacophorinae) from Van Ban District, Lao Cai Province, northern Vietnam. Russian Journal of Herpetology, 26 (6), 325–334. https://doi.org/10.30906/1026-2296-2019-26-6-325-334
    Lathrop, A., Murphy, R.W., Orlov, N. & Ho, C.T. (1998) Two new species of Leptolalax (Anura: Megophryidae) from northern Vietnam. Amphibia-Reptilia, 19 (3), 253–267. https://doi.org/10.1163/156853898X00160
    Li, S.Z., Liu, J., Wei, G. & Wang, B. (2020) A new species of the Asian leaf litter toad genus Leptobrachella (Amphibia, Anura, Megophryidae) from southwest China. ZooKeys, 943, 91–118. https://doi.org/10.3897/zookeys.943.51572
    Liu, C.C. (1950) Amphibians of Western China. Fieldiana: Zoological Memoirs, 2, 1–400, 10 pl. https://doi.org/10.5962/bhl.part.4737
    Liu, C.C., Hu, S.Q. & Yang, F.H. (1960) Amphibia of Yunnan collected in 1958. Acta Zoologica Sinica, 12, 149–174.
    Luo, T., Xiao, N., Gao, K. & Zhou, J. (2020) A new species of Leptobrachella (Anura, Megophryidae) from Guizhou Province, China. ZooKeys, 923, 115–140. https://doi.org/10.3897/zookeys.923.47172
    Luong, A.M., Pham, A.V., Nguyen, T.T. & Nguyen, T.Q. (2019) First record of Megophrys gigantica Liu, Hu & Yang, 1960 (Anura: Megophryidae) from Vietnam. Russian Journal of Herpetology, 26, 201–204. https://doi.org/10.30906/1026-2296-2019-26-4-201-204
    Lyu, J.C., Dai, L.L., Wei, P.F., He, Y.H., Yuan, Z.Y., Shi, W.L., Zhou, S.L., Ran, S.Y., Kuang, Z.F., Guo, X., Wei, G. & Yuan, G. (2020) A new species of the genus Leptobrachella Smith, 1925 (Anura, Megophryidae) from Guizhou, China. ZooKeys, 1008, 139–157. https://doi.org/10.3897/zookeys.1008.56412
    Mahony, S., Foley, N.M., Biju, S.D. & Teeling, E.C. (2017) Evolutionary history of the Asian Horned Frogs (Megophryinae): integrative approaches to timetree dating in the absence of a fossil record. Molecular biology and evolution, 34 (3), 744–771. https://doi.org/10.1093/molbev/msw267
    Mathew, R. & Sen, N. (2010) Description of a new species of Leptobrachium Tschudi, 1838 (Amphibia: Anura: Megophryidae) from Meghalaya, India. Records of the Zoological Survey of India, 109, 91–108.
    Matsui, M. (2006) Three new species of Leptolalax from Thailand (Amphibia, Anura, Megophryidae). Zoological Science, 23 (9), 821–830. https://doi.org/10.2108/zsj.23.821
    Matsui, M., Hamidy, A., Murphy, R.W., Khonsue, W., Yambun, P., Shimada, T., Ahmad, N., Belabut, D.M. & Jiang, J.P. (2010) Phylogenetic relationships of megophryid frogs of the genus Leptobrachium (Amphibia, Anura) as revealed by mtDNA gene sequences. Molecular Phylogenetics and Evolution, 56 (1), 259–272. https://doi.org/10.1016/j.ympev.2010.03.014
    Matsui, M., Nishikawa, K. & Yambun, P. (2014) A new Leptolalax from the mountains of Sabah, Borneo (Amphibia, Anura, Megophryidae). Zootaxa, 3753 (3), 440–452. https://doi.org/10.11646/zootaxa.3753.5.3
    Matsui, M., Ohler, A., Eto, K. & Nguyen, T.T. (2017a) Distinction of Gracixalus carinensis from Vietnam and Myanmar, with description of a new species. Alytes, 33 (1–4), 25–37.
    Matsui, M., Eto, K., Nishikawa, K., Hamidy, A., Belabut, D., Ahmad, N., Panha, S., Khonsue, W. & Grismer, L.L. (2017b) Mitochondrial phylogeny of Leptolalax from Malay Peninsula and Leptobrachella (Anura, Megophryidae). Current herpetology, 36 (1), 11–21. https://doi.org/10.5358/hsj.36.11
    Minh, B.Q., Schmidt, H.A., Chernomor, O., Schrempf, D., Woodhams, M.D., Von Haeseler, A. & Lanfear, R. (2020) IQ-TREE 2: New models and efficient methods for phylogenetic inference in the genomic era. Molecular Biology and Evolution, 37 (5), 1530−1534. https://doi.org/10.1093/molbev/msaa015
    Nguyen, L.T., Poyarkov, N.A., Le, D.T., Vo, B.D., Phan, H.T., Duong, T.V., Murphy, R.W. & Nguyen, S.N. (2018) A new species of Leptolalax (Anura: Megophryidae) from Son Tra Peninsula, central Vietnam. Zootaxa, 4388 (1), 1–21. https://doi.org/10.11646/zootaxa.4388.1.1
    Nguyen, S.V., Ho, T.C. & Nguyen, T.Q. (2009) Herpetofauna of Vietnam, Edition Chimaira, Frankfurt am Main, 768 pp.
    Nguyen, T.Q., Phung, T.M., Le, M.D., Ziegler, T. & Böhme, W. (2013) First record of the genus Oreolalax (Anura: Megophryidae) from Vietnam with description of a new species. Copeia, 2013, 213–222. https://doi.org/10.1643/CH-12-021
    Nguyen, L.T., Tapley. B., Cutajar, T., Nguyen, C.T., Portway, C., Harding, L., Luong, H.V. & Rowley, J.J.L. (2020) A description of the tadpole of the Critically Endangered Botsford’s leaf-litter frog (Leptobrachella botsfordi) with comments on the distribution and conservation status of the species. Zootaxa, 4860 (2), 293–300. https://doi.org/10.11646/zootaxa.4860.2.10
    Oberhummer, E., Barten, C., Schweizer, M., Das, I., Haas, A. & Hertwig, S.T. (2014) Description of the tadpoles of three rare species of megophryid frogs (Amphibia: Anura: Megophryidae) from Gunung Mulu, Sarawak, Malaysia. Zootaxa, 3835 (1), 59–79. https://doi.org/10.11646/zootaxa.3835.1.3
    Ohler, A., Marquis, O., Swan, S. & Grosjean, S.T. (2000) Amphibian biodiversity of Hoang Lien Nature Reserve (Lao Cai Province, northern Vietnam) with description of two new species. Herpetozoa, 13 (1/2), 71–87.
    Ohler, A., Wollenberg, K.C., Grosjean, S., Hendrix, R., Vences, M., Ziegler, T. & Dubois, A. (2011) Sorting out Lalos: description of new species and additional taxonomic data on megophryid frogs from northern Indochina (genus Leptolalax, Megophryidae, Anura). Zootaxa, 3147 (1), 1–83. https://doi.org/10.11646/zootaxa.3147.1.1
    Posada, D. & Crandall, K. A. (1998). Modeltest: testing the model of DNA substitution. Bioinformatics, 14 (9), 817–818. https://doi.org/10.1093/bioinformatics/14.9.817
    Poyarkov, N.A., Rowley, J.J., Gogoleva, S.I., Vassilieva, A.B., Galoyan, E.A. & Orlov, N.L. (2015) A new species of Leptolalax (Anura: Megophryidae) from the western Langbian Plateau, southern Vietnam. Zootaxa, 3931 (2), 221–252. https://doi.org/10.11646/zootaxa.3931.2.3
    Qian, T.Y., Xia, X., Cao, Y., Xiao, N.W. & Yang, D.D. (2020) A new species of Leptobrachella (Anura: Megophryidae) Smith, 1925 from Wuling Mountains in Hunan Province, China. Zootaxa, 4816 (4) 491–526. https://doi.org/10.11646/zootaxa.4816.4.4
    Ronquist, F., Teslenko, M., Van Der Mark, P., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology, 61, 539–542. https://doi.org/10.1093/sysbio/sys029
    Rowley, J.J. & Cao, T.T. (2009) A new species of Leptolalax (Anura: Megophryidae) from central Vietnam. Zootaxa, 2198 (1), 51–60. https://doi.org/10.11646/zootaxa.2198.1.5
    Rowley, J.J., Stuart, B.L., Richards, S.J., Phimmachak, S. & Sivongxay, N. (2010) A new species of Leptolalax (Anura: Megophryidae) from Laos. Zootaxa, 2681 (1), 35–46. https://doi.org/10.11646/zootaxa.2681.1.3
    Rowley, J.J., Stuart, B.L., Neang, T. & Emmett, D.A. (2010b) A new species of Leptolalax (Anura: Megophryidae) from northeastern Cambodia. Zootaxa, 2567 (1), 57–68. https://doi.org/10.11646/zootaxa.2567.1.3
    Rowley, J.J., Hoang, D.H., Le, T.T.D., Dau, Q.V. & Cao, T.T. (2010c) A new species of Leptolalax (Anura: Megophryidae) from Vietnam and further information on Leptolalax tuberosus. Zootaxa, 2660 (1), 33–45.
    Rowley, J.J., Le, D.T.T., Tran, D.T.A. & Hoang, H.D. (2011) A new species of Leptolalax (Anura: Megophryidae) from southern Vietnam. Zootaxa, 2796 (1), 15–28. https://doi.org/10.11646/zootaxa.2796.1.2
    Rowley, J.J., Hoang, H.D., Dau, V.Q., Le, D.T.T. & Cao, T.T. (2012) A new species of Leptolalax (Anura: Megophryidae) from central Vietnam. Zootaxa, 3321 (1), 56–68. https://doi.org/10.11646/zootaxa.3321.1.4
    Rowley, J.J., Dau, V.Q. & Nguyen T.T. (2013) A new species of Leptolalax (Anura: Megophryidae) from the highest mountain in Indochina. Zootaxa, 3737 (4), 415–428. https://doi.org/10.11646/zootaxa.3737.4.5
    Rowley, J.J., Stuart, B.L., Neang, T., Hoang, H.D., Dau, V.Q., Nguyen, T.T. & Emmett, D.A. (2015a) A new species of Leptolalax (Anura: Megophryidae) from Vietnam and Cambodia. Zootaxa, 4039 (3), 401–417. https://doi.org/10.11646/zootaxa.4039.3.1
    Rowley, J.J., Tran, D.T., Frankham, G.J., Dekker, A.H., Le, D.T., Nguyen, T.Q., Dau, V.Q. & Hoang, H.D. (2015b) Undiagnosed cryptic diversity in small, microendemic frogs (Leptolalax) from the Central Highlands of Vietnam. PloS One, 10 (5), e0128382. https://doi.org/10.1371/journal.pone.0128382
    Rowley, J.J., Tran, D.T.A., Le, D.T.T., Dau, V.Q., Peloso, P.L.V., Nguyen, T.Q., Hoang, H.D., Nguyen, T.T. & Ziegler, T. (2016) Five new, microendemic Asian Leaf-litter Frogs (Leptolalax) from the southern Annamite mountains, Vietnam. Zootaxa, 4085 (1), 63–102. https://doi.org/10.11646/zootaxa.4085.1.3
    Rowley, J.J., Dau, V.Q., Hoang, H.D., Le, D.T., Cutajar, T.P. & Nguyen, T.T. (2017a) A new species of Leptolalax (Anura: Megophryidae) from northern Vietnam. Zootaxa, 4243 (3), 544–564. https://doi.org/10.11646/zootaxa.4243.3.7
    Rowley, J.J., Dau, V.Q. & Cao, T.T. (2017b) A new species of Leptolalax (Anura: Megophryidae) from Vietnam. Zootaxa, 4273 (1), 61–79. https://doi.org/10.11646/zootaxa.4273.1.5
    Sengupta, S., Sailo, S., Lalremsanga, H.T., Das, A. & Das, I. (2010) A new species of Leptolalax (Anura: Megophryidae) from Mizoram, north-eastern India. Zootaxa, 2406 (1), 57–68. https://doi.org/10.11646/zootaxa.2406.1.3
    Shi, S., Hou, Y., Song, Z., Jiang, J. & Wang, B. (2021) A new leaf litter toad of Leptobrachella Smith, 1925 (Anura, Megophryidae) from Sichuan Province, China with supplementary description of L. oshanensis. Asian Herpetological Research, 12 (2), 143–166. https://doi.org/10.16373/j.cnki.ahr.200118
    Smith, M.A. (1925) Contributions to the herpetology of Borneo. Sarawak Museum Journal, 3 (11), 15–34.
    Stuart, B.L. & Rowley, J.J. (2020) A new Leptobrachella (Anura: Megophryidae) from the Cardamom Mountains of Cambodia. Zootaxa, 4834 (4), 556–572. https://doi.org/10.11646/zootaxa.4834.4.4
    Sung, Y.H., Yang, J. & Wang, Y. (2014) A new species of Leptolalax (Anura: Megophryidae) from southern China. Asian Herpetological Research, 5 (2), 80–90. https://doi.org/10.3724/SP.J.1245.2014.00080
    Tapley, B., Cutajar, T., Nguyen. L.T., Portway, C., Mahony, S., Nguyen, C.T., Harding, L., Luong, H.V. & Rowley, J.J.L. (2021) A new potentially Endangered species of Megophrys from Mount Ky Quan San, northwest Vietnam. Journal of Natural History, 54, 2543–2575. https://doi.org/10.1080/00222933.2020.1856952
    Taylor, E.H. (1962) The amphibian fauna of Thailand. University of Kansas Science Bulletin, 43, 265–599. https://doi.org/10.5962/bhl.part.13347
    Veith, M., Fromhage, L., Kosuch, J. & Vences, M. (2006) Historical biogeography of Western Palaearctic pelobatid and pelodytid frogs: a molecular phylogenetic perspective. Contributions to Zoology, 75 (03–04), 109–120. https://doi.org/10.1163/18759866-0750304001
    Vences, M., Thomas, M., Van der Meijden, A., Chiari, Y. & Vieites, D.R. (2005) Comparative performance of the 16S rRNA gene in DNA barcoding of amphibians. Frontiers in zoology, 2 (1), 1–12. https://doi.org/10.1186/1742-9994-2-5
    Wang, J., Yang, J., Li, Y., Lyu, Z., Zeng, Z., Liu, Z., Ye, Y. & Wang, Y. (2018) Morphology and molecular genetics reveal two new Leptobrachella species in southern China (Anura, Megophryidae). ZooKeys, 776, 105–137. https://doi.org/10.3897/zookeys.776.22925
    Wang, J., Li, Y.L., Li, Y., Chen, H.H., Zeng, Y.J., Shen, J.M. & Wang, Y.Y. (2019) Morphology, molecular genetics, and acoustics reveal two new species of the genus Leptobrachella from northwestern Guizhou Province, China (Anura, Megophryidae). ZooKeys, 848, 119–154. https://doi.org/10.3897/zookeys.848.29181
    Wang, J., Lyu, Z.T., Qi, S., Zeng, Z.C., Zhang, W.X., Lu, L.S. & Wang, Y.Y. (2020) Two new Leptobrachella species (Anura, Megophryidae) from the Yunnan-Guizhou Plateau, southwestern China. ZooKeys, 995, 97–125. https://doi.org/10.3897/zookeys.995.55939
    Xiang, T., Wang, B., Liang, X., Jiang, J., Li, C. & Xie, F. (2013) Complete mitochondrial genome of Paramegophrys oshanensis (Amphibia, Anura, Megophryidae). Mitochondrial DNA, 24 (5), 472–474. https://doi.org/10.3109/19401736.2013.766183
    Yang, D.T., Ma, D.S., Li, F.L. & Chen, H.J.J. (1983) Descriptions of two new pelobatid toads from Yunnan. Acta Zootaxonomica Sinica, 8, 323–327.
    Yang, J.H., Wang, Y.Y., Chen, G.L. & Rao, D.Q. (2016) A new species of the genus Leptolalax (Anura: Megophryidae) from Mt. Gaoligongshan of western Yunnan Province, China. Zootaxa, 4088 (3), 379–394. https://doi.org/10.11646/zootaxa.4088.3.4
    Yang, J.H., Zeng, Z.C. & Wang, Y.Y. (2018) Description of two new sympatric species of the genus Leptolalax (Anura: Megophryidae) from western Yunnan of China. PeerJ, 6, e4586. https://doi.org/10.7717/peerj.4586
    Yuan, Z., Sun, R., Chen, J. Rowley, J.J., Wu, Z., Hou, S. & Che, J. (2017) A new species of the genus Leptolalax (Anura: Megophryidae) from Guangxi, China. Zootaxa, 4300 (4), 551–570. https://doi.org/10.11646/zootaxa.4300.4.5
    Zheng, Y.C., Mo, B.H., Liu, Z.J. & Zeng, X.M. (2004) Phylogenetic relationships of megophryid genera (Anura: Megophryidae) based on partial sequences of mitochondrial 16 S rRNA gene. Zoological Research, 25 (3), 205–213.

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How to Cite

NGUYEN, L. T. ., TAPLEY, B. ., NGUYEN, C. T. ., LUONG, H. V. ., & ROWLEY, J. J. . (2021). A new species of <em>Leptobrachella</em> (Anura, Megophryidae) from Mount Pu Ta Leng, northwest Vietnam. Zootaxa, 5016(3), 301–332. https://doi.org/10.11646/zootaxa.5016.3.1