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Type: Article
Published: 2021-11-18
Page range: 517-532
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New species of flea-toad, genus Brachycephalus (Anura: Brachycephalidae) from the Atlantic Forest of Espírito Santo, Brazil

Universidade Federal do ABC, Centro de Ciências Naturais e Humanas, Av. dos Estados, 5001, Santo André, 09210-580, São Paulo, Brazil.
Universidade Federal do Espírito Santo, Museu de História Natural do Sul do Estado do Espírito Santo, Jerônimo Monteiro, 29550-000, Espírito Santo, Brazil. Herpeto Capixaba, Enseada Azul, Guarapari, 29206-090, Espírito Santo, Brazil.
Universidade de São Paulo, Instituto de Biociências, Caixa Postal 11461, CEP 05422-970, São Paulo, Brazil.
Universidade Federal do ABC, Centro de Ciências Naturais e Humanas, Av. dos Estados, 5001, Santo André, 09210-580, São Paulo, Brazil.
Amphibia

Abstract

We describe a new species of dull-colored flea-toad, genus Brachycephalus, from the Atlantic Forest of Caparaó mountains in southeastern Brazil. The new species is characterized by its diminutive size, “leptodactyliform” body, brownish color with an inverted V-shaped dark mark on dorsum, skin smooth, hyperossification and dorsal shield absent, linea masculinea absent, Fingers I and IV vestigial, Toe I externally absent, Toe II reduced but functional, Toes III and IV with pointed tips, Toe V vestigial, and ventral color uniformly brown. It is a leaf litter dweller, known only from type locality in the humid forests on the eastern slopes of Parque Nacional do Caparaó mountains, a protected area in the states of Espírito Santo and Minas Gerais, southeastern Brazil. It is the third flea-toad occurring in the state of Espírito Santo recovered as sister to all other Brachycephalus distributed from the state of São Paulo northward in the Atlantic Forest.

 

References

  1. Bornschein, M.R., Firkowski, C.R., Belmonte-Lopes, R., Corrêa, L., Ribeiro, L.F., Morato, S.A.A, Antoniazzi-Jr, R.L., Reinert, B.L., Meyer, A.L.S., Cini, F.A. & Pie, M.R. (2016) Geographical and altitudinal distribution of Brachycephalus (Anura: Brachycephalidae) endemic to the Brazilian Atlantic Rainforest. PeerJ, 4, e2490. https://doi.org/10.7717/peerj.2490.
    Bornschein, M.R., Ribeiro, L.F., Teixeira, L., Belmonte-Lopes, R., de Moraes, L.A., Corrêa, L., Mauriìcio, G.N., Nadaline, J. & Pie, M.R. (2021) A review of the diagnosis and geographical distribution of the recently described flea toad Brachycephalus sulfuratus in relation to B. hermogenesi (Anura: Brachycephalidae). PeerJ, 9, e10983. https://doi.org/10.7717/peerj.10983
    Cadena, C.D., Kozak, K.H., Gomez, J.P., Parra, J.L., McCain, C.M., Bowie, R.C.K., Carnaval, A.C., Moritz, C., Rahbek, C., Roberts, T.E., Sanders, N.J., Schneider, C.J., VanDerWal, J., Zamudio, K.R. & Graham, C.H. (2012) Latitude, elevational climatic zonation and speciation in New World vertebrates. Proceedings of the Royal Society B, 279 (1726), 194–201. http://doi.org/10.1098/rspb.2011.0720
    Campos, L.A., Silva, H.R. & Sebben, A. (2010) Morphology and development of additional bony elements in the genus Brachycephalus (Anura: Brachycephalidae). The Biological Journal of the Linnean society, 99 (4), 752–767. https://doi.org/10.1111/j.1095-8312.2010.01375.x
    Clemente-Carvalho, R.B., Antoniazzi, M.M., Jared, C., Haddad, C.F., Alves, A.C., Rocha, H.S., Pereira, G.R., Oliveira, D.F., Lopes, R.T. & dos Reis, S.F. (2009) Hyperossification in miniaturized toadlets of the genus Brachycephalus (Amphibia: Anura: Brachycephalidae): microscopic structure and macroscopic patterns of variation. Journal of Morphology, 270 (11), 1285–1295. https://doi.org/10.1002/jmor.10755
    Clemente-Carvalho, R.B.G., Klaczko, J., Perez, S.I., Alves, A.C.R., Haddad, C.F.B. & dos Reis, S.F. (2011) Molecular phylogenetic relationships and phenotypic diversity in miniaturized toadlets, genus Brachycephalus (Amphibia: Anura: Brachycephalidae). Molecular Phylogenetics and evolution, 61 (1), 79–89. https://doi.org/10.1016/j.ympev.2011.05.017
    Condez, T.H., Clemente-Carvalho, R.B., Haddad, C.F.B. & dos Reis, S.F. (2014) A New Species of Brachycephalus (Anura: Brachycephalidae) from the Highlands of the Atlantic Forest, Southeastern Brazil. Herpetologica, 70, 89–99. https://doi.org/10.1655/HERPETOLOGICA-D-13-00044
    Condez, T.H., Monteiro, J.D.C., Comitti, E.J., Garcia, P.D.A., Amaral, I.B. & Haddad, C.F.B. (2016) A new species of flea-toad (Anura: Brachycephalidae) from southern Atlantic Forest, Brazil. Zootaxa, 4083 (1), 40–56. https://doi.org/10.11646/zootaxa.4083.1.2
    Condez, T.H., Haddad, C.F. & Zamudio, K.R. (2020) Historical biogeography and multi-trait evolution in miniature toadlets of the genus Brachycephalus (Anura: Brachycephalidae). Biological Journal of the Linnean Society, 129 (3), 664–686. https://doi.org/10.1093/biolinnean/blz200
    Ferreira, R.B., Beard, K.H. & Crump, M.L. (2016) Breeding guild determines frog distributions in response to edge effects and habitat conversion in the Brazil’s Atlantic Forest. PLoS ONE, 11, e0156781. https://doi.org/10.1371/journal.pone.0156781
    Ferreira, R.B., Mônico, A.T., Silva, E.T., Lirio, F.C.F., Zocca, C., Mageski, M.M., Tonini, J.F.R., Beard, K.H., Duca, C. & Silva-Soares, T. (2019) Amphibians of Santa Teresa, Brazil: the hotspot further evaluated. Zookeys, 857, 139–162. https://doi.org/10.3897/zookeys.857.30302
    Fitzinger, L.J.F.J. (1826) Neue Classification der Reptilien nach ihren Natürlichen Verwandtschaften nebst einer Verwandtschafts-Tafel und einem Verzeichnisse der Reptilien-Sammlung des K. K. Zoologisch Museum’s zu Wien. J. G. Heubner, Wien, 66 pp. https://doi.org/10.5962/bhl.title.4683
    Folly, M., Amaral, L.C., Carvalho-e-Silva, S.P. & Pombal Jr., J.P. (2020) Rediscovery of the toadlet Brachycephalus bufonoides Miranda-Ribeiro, 1920 (Anura: Brachycephalidae) with osteological and acoustic descriptions. Zootaxa, 4819 (2), 265–294. https://doi.org/10.11646/zootaxa.4819.2.3
    Frost, D.R. (2021) Amphibian Species of the World: An Online Reference. American Museum of Natural History, New York, New York. 21 May 2019. Available from: http://research.amnh.org/herpetology/amphibia/index.html (accessed 30 April 2021)
    Giaretta, A.A. & Sawaya, R.J. (1998) Second species of Psyllophryne (Anura: Brachycephalidae). Copeia, 4, 985–987. https://doi.org/10.2307/1447345
    ICMBio [Instituto Chico Mendes de Conservação da Biodiversidade] (2015) Plano de Manejo para o Parque Nacional do Caparaó. Brasília, 537 pp. Available from: https://www.icmbio.gov.br/parnacapara o/plano-de-manejo (accessed 4 January 2021)
    IPHAN [Instituto do Patrimônio Histórico e Artístico Nacional] (2017) Mapa etno-histórico do Brasil e regiões adjacentes. 2nd Edition. Instituto Brasileiro de Geografia e Estatística. Brasília, 120 pp.
    IUCN (2021) The IUCN Red List of Threatened Species. Version 2021-1. IUCN Red List Unit, Cambridge. Available from: https://www.iucnredlist.org (accessed 22 April 2021)
    Izecksohn, E. (1971). Nôvo gênero e nova espécie de Brachycephalidae do estado do Rio de Janeiro, Brasil (Amphibia, Anura). Boletim do Museu Nacional, Rio de Janeiro, Nova Série, Zoologia, 280, 1–12.
    Janzen, D.H. (1967) Why mountain passes are higher in the tropics. The American Naturalist, 101 (919), 233–249. https://doi.org/10.1086/282487
    Jetz, W. & Pyron, R.A. (2018) The interplay of past diversification and evolutionary isolation with present imperilment across the amphibian tree of life. Nature Ecology & Evolution, 2, 850–858. https://doi.org/10.1038/s41559-018-0515-5
    Kearse, M., Moir, R., Wilson, A., Stones-Havas, S., Cheung, M., Sturrock, S., Buxton, S., Cooper, A., Markowitz, S., Duran, C., Thierer, T., Ashton, B., Meintjes, P. & Drummond, A. (2012) Geneious Basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics, 28 (12), 1647–1649. https://doi.org/10.1093/bioinformatics/bts199
    Kozak, K.H. & Wiens, J.J. (2007) Climatic zonation drives latitudinal variation in speciation mechanisms. Proceedings of the Royal Society B, 274 (1628), 2995–3003. https://doi.org/10.1098/rspb.2007.1106
    Kumar, S., Stecher, G., Li, M., Knyaz, C. & Tamura, K. (2018) MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms. Molecular Biology and Evolution, 35, 1547–1549. https://doi.org/10.1093/molbev/msy096
    Lanfear, R., Frandsen, P.B., Wright, A.M., Senfeld, T. & Calcott, B. (2016) PartitionFinder 2: new methods for selecting partitioned models of evolution for molecular and morphological phylogenetic analyses. Molecular Biology and Evolution, 34, 772–773. https://doi.org/10.1093/molbev/msw260
    Li, W., Cowley, A., Uludag, M., Gur, T., McWilliam, H., Squizzato, S., Park, Y.M., Buso, N. & Lopez, R. (2015) The EMBL-EBI bioinformatics web and programmatic tools framework. Nucleic acids research, 43 (W1), W580–W584. https://doi.org/10.1093/nar/gkv279
    Miller, M.A., Pfeiffer, W. & Schwartz, T. (2010) Creating the CIPRES Science Gateway for inference of large phylogenetic trees. Proceedings of the Gateway Computing Environments Workshop. The CIPRES Science Gateway, New Orleans. Available from: http://www.phylo.org (accessed 20 March 2021) https://doi.org/10.1109/GCE.2010.5676129
    Moritz, C., Patton, J.L., Schneider, C.J. & Smith, T.B. (2000) Diversification of rainforest faunas: an integrated molecular approach. Annual review of ecology and systematics, 31 (1), 533–563. https://doi.org/10.1146/annurev.ecolsys.31.1.533
    Napoli, M.F., Caramaschi, U., Cruz, C.A.G. & Dias, I.R. (2011) A new species of flea-toad, genus Brachycephalus Fitzinger (Amphibia: Anura: Brachycephalidae), from the Atlantic rainforest of southern Bahia, Brazil. Zootaxa, 2739 (1), 33–40. https://doi.org/10.11646/zootaxa.2739.1.3
    Nunes, I., Guimarães, C.S., Moura, P.H.A.G., Pedrozo, M., Moroti, M.T., Castro, L.M., Stuginski, D.R. & Muscat, E. (2021) Hidden by the name: A new fluorescent pumpkin toadlet from the Brachycephalus ephippium group (Anura: Brachycephalidae). PLoS ONE, 16, e0244812. https://doi.org/10.1371/journal.pone.0244812
    Padial, J.M., Grant, T. & Frost, D.R. (2014) Molecular systematics of terraranas (Anura: Brachycephaloidea) with an assessment of the effects of alignment and optimality criteria. Zootaxa, 3825 (1), 1–132. https://doi.org/10.11646/zootaxa.3825.1.1
    Pie, M.R., Meyer, A.L.S., Firkowski, C.R., Ribeiro, L.F. & Bornschein, M.R. (2013) Understanding the mechanisms underlying the distribution of microendemic montane frogs (Brachycephalus spp., Terrarana: Brachycephalidae) in the Brazilian Atlantic Rainforest. Ecological Modelling, 250, 165–176. https://doi.org/10.1016/j.ecolmodel.2012.10.019
    Pie, M.R., Ribeiro, L.F., Confetti, A.E., Nadaline, M.J. & Bornschein, M.R. (2018) A new species of Brachycephalus (Anura: Brachycephalidae) from southern Brazil. PeerJ, 6, e5683. https://doi.org/10.7717/peerj.5683
    Polato, N.R., Gill, B.A., Shah, A.A., Gray, M.M., Casner, K.L., Barthelet, A., Messer, P.W., Simmons, M.P., Guayasamin, J.M., Encalada, A.C., Kondratieff, B.C., Flecker, A.S., Thomas, S.A., Ghalambor, C.K., Poff, N.L., Funk, W.C. & Zamudio, K.R. (2018) Narrow thermal tolerance and low dispersal drive higher speciation in tropical mountains. Proceedings of the National Academy of Sciences, 115 (49), 12471–12476. https://doi.org/10.1073/pnas.1809326115
    Pyron, R.A. & Wiens, J.J. (2011) A large-scale phylogeny of Amphibia including over 2,800 species, and a revised classification of extant frogs, salamanders, and caecilians. Molecular Phylogenetics and Evolution, 61, 543–583. https://doi.org/10.1016/j.ympev.2011.06.012
    Rambaut, A., Drummond, A.J., Xie, D., Baele, G. & Suchard, M.A. (2018) Posterior summarization in Bayesian phylogenetics using Tracer 1.7. Systematic biology, 67 (5), 901. https://doi.org/10.1093/sysbio/syy032
    Ribeiro, L.F., Bornschein, M.R., Belmonte-Lopes, R., Firkowski, C.R., Morato, S.A.A. & Pie, M.R. (2015) Seven new microendemic species of Brachycephalus (Anura: Brachycephalidae) from southern Brazil. PeerJ, 3, e1011. https://doi.org/10.7717/peerj.1011
    Ribeiro, L.F., Blackburn, D.C., Stanley, E.L., Pie, M.R. & Bornschein, M.R. (2017) Two new species of the Brachycephalus pernix group (Anura: Brachycephalidae) from the state of Paraná, southern Brazil. PeerJ, 5, e3603. https://doi.org/10.7717/peerj.3603
    Ronquist, F., Teslenko, M., Van Der Mark, P., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) Mrbayes 3.2: Efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology, 61, 539–542. https://doi.org/10.1093/sysbio/sys029
    Schneider, C.A., Rasband, W.S. & Eliceiri, K.W. (2012) NIH Image to ImageJ: 25 years of image analysis. Nature methods, 9 (7), 671–675. https://doi.org/10.1038/nmeth.2089
    Silva-Soares, T., Malagoli, L.R., Cruz, C.A.G. & Caramaschi, U. (2018) On the taxonomy and natural history of the secretive Ischnocnema lactea Miranda-Ribeiro, 1923) (Anura: Brachycephaloidea: Brachycephalidae). Zootaxa, 4444 (2), 137–153. https://doi.org/10.11646/zootaxa.4444.2.3
    Spix, J.B.D. (1824) Animalia nova sive species novae Testudinum et Ranarum, quas in itinere per Brasiliam annis MDCCCXVII–MDCCCXX jussu et auspiciis Maximiliani Josephi I. Bavariae Regis. Typis Franc. Seraph. Hübschmanni, Monachii, 53 pp., XXII tabs. https://doi.org/10.5962/bhl.title.3665
    Vaidya, G., Lohman, D.J. & Meier, R. (2011) SequenceMatrix: concatenation software for the fast assembly of multi-gene datasets with character set and codon information. Cladistics, 27, 171–180. https://doi.org/10.1111/j.1096-0031.2010.00329.x
    Verdade, V., Carnaval, A., Rodrigues, M., Schiesari, L., Pavan, D. & Bertoluci, J. (2011) Decline of Amphibians in Brazil. In: Heatwole, H. (Ed.), Amphibian Biology. Vol. 9. Status of Decline of Amphibians: Western Hemisphere. Part 2. Ecuador and Colombia. Surrey Beatty & Sons, Baulkham Hills, pp. 85–127.
    Watters, J.L., Cummings, S.T., Flanagan, R.L. & Siler, C.D. (2016) Review of morphometric measurements used in anuran species descriptions and recommendations for a standardized approach. Zootaxa, 4072 (4), 477–495. http://doi.org/10.11646/zootaxa.4072.4.6
    Wollenberg, K.C., Vieites, D.R., Glaw, F. & Vences, M. (2011) Speciation in little: the role of range and body size in the diversification of Malagasy mantellid frogs. BMC Evolutionary Biology, 11, 217. https://doi.org/10.1186/1471-2148-11-217
    Yeh, J. (2002) The effect of miniaturized body size on skeletal morphology in frogs. Evolution, 56 (3), 628–641. https://doi.org/10.1111/j.0014-3820.2002.tb01372.x
    Zornosa-Torres, C., Augusto-Alves, G., Lyra, M.L., Silva Júnior, J.C.D., Garcia, P.C., Leite, F., Verdade, V., Rodrigues, M.T., Gasparini, J.L., Haddad, C.F.B.. & Toledo, L.F. (2020) Anuros do Parque Nacional do Caparaó e arredores, sudeste brasileiro. Biota Neotropica, 20 (3), e20190882. https://doi.org/10.1590/1676-0611-BN-2019-0882

  2.