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Type: Article
Published: 2021-12-17
Page range: 201-222
Abstract views: 685
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A new species of Ischnocnema (Anura: Brachycephalidae) from the mountainous region of Atlantic Forest, southeastern Brazil, with a new phylogeny and diagnose for Ischnocnema parva series

Herpeto Capixaba, Enseada Azul, Guarapari, CEP 29.206–090, Espírito Santo, Brazil. 2 Universidade Federal do Espírito Santo, Museu de História Natural do Sul do Estado do Espírito Santo, Jerônimo Monteiro, CEP 29550–000, Espírito Santo, Brazil.
Instituto Nacional da Mata Atlântica, Av. José Ruschi, nº 4, CEP 29650–000, Santa Teresa, ES, Brazil. 4 Projeto Bromelias Instituto Marcos Daniel, Av. Eugenio Pacheco de Queirós, s/n, CEP 29090–160, Vitória, ES, Brazil. CZZ
Projeto Bromelias Instituto Marcos Daniel, Av. Eugenio Pacheco de Queirós, s/n, CEP 29090–160, Vitória, ES, Brazil. Universidade Federal do Espírito Santo, Departamento de Biologia, CCHN, Laboratório de Mastozoologia e Biogeografia, Av. Fernando Ferrari, nº 514, CEP 29075–910, Vitória, ES, Brazil.
Instituto Nacional da Mata Atlântica, Av. José Ruschi, nº 4, CEP 29650–000, Santa Teresa, ES, Brazil. 4 Projeto Bromelias Instituto Marcos Daniel, Av. Eugenio Pacheco de Queirós, s/n, CEP 29090–160, Vitória, ES, Brazil.
Universidade Federal do Rio de Janeiro, Museu Nacional, Departamento de Vertebrados, Quinta da Boa Vista, São Cristóvão, CEP 20940–040, Rio de Janeiro, RJ, Brazil.
Universidade Federal do Rio de Janeiro, Museu Nacional, Departamento de Vertebrados, Quinta da Boa Vista, São Cristóvão, CEP 20940–040, Rio de Janeiro, RJ, Brazil.
Amphibia Ischnocnema crassa sp. nov. biodiversity Espírito Santo morphology taxonomy natural history conservation

Abstract

We describe a new species of Rain Frog from Brazil’s Atlantic Forest, which harbors high richness, endemism and threatened species of anurans. Ischnocnema crassa sp. nov. is a leaf litter breeder from the montane rainforest of Santa Teresa, state of Espírito Santo, southeastern Brazil. Remarkably, the new species is so far unique amongst Ischnocnema for having moderate hand webbing between fingers. Ischnocnema crassa sp. nov. is the third species belonging to I. parva series and we present a new phylogeny based on molecular data and propose a new diagnosis for the series. We provide field and laboratory observations regarding amplexus, antipredator mechanisms, microhabitat preferences and conservation remarks.  Although Santa Teresa is one of the most sampled areas in Brazil, new species are often discovered. Santa Teresa is a priority for amphibian conservation because it harbors 109 species of anurans (9% of Brazil’s species).

 

References

  1. Alvares, C.A., Stape, J.L., Sentelhas, P.C., Gonçalves, J.L.M. & Sparoveck, G. (2013) Köppen climate classification map for Brazil. Meteorologische Zeitschrift, 22, 711–728. https://doi.org/10.1127/0941-2948/2013/0507
    AVMA [American and Veterinary Medical Association] (2013) Guidelines for the euthanasia of animals. American and Veterinary Medical Association, Schaumberg, Illinois, 102 pp.
    Bokermann, W.C.A. (1975) Três espécies novas de Eleutherodactylus do sudeste da Bahia, Brasil (Anura: Leptodactylidae). Revista Brasileira de Biologia, 34, 11–18.
    Boulenger, G.A. (1888) On some reptiles and batrachians from Iguarasse, Pernambuco. Annals and Magazine of Natural History, 6, 40–43. https://doi.org/10.1080/00222938809460873
    Bruford, M.W., Hanotte, O., Brookfield, J.F.Y. & Burke, T. (1992) Single-Locus and DNA fingerprinting. In: Hoelzel, A.R. (Ed.), Molecular genetic analyses of populations. A practical approach. IRL Press, Oxford, 225–269.
    Brusquetti, F.A., Thomé, M.T.C., Canedo, C., Condez, T.H. & Haddad, C.F.B. (2013) A new species of Ischnocnema parva species series (Anura: Brachycephalidae) from northern state of Rio de Janeiro, Brazil. Herpetologica, 69, 175–185. https://doi.org/10.1655/herpetologica-d-12-00050 
    Canedo, C. & Haddad, C.F.B. (2012) Phylogenetic relationships within anuran clade Terrarana, with emphasis on the placement of Brazilian Atlantic rainforest frogs genus Ischnocnema (Anura: Brachycephalidae). Molecular Phylogenetics and Evolution, 65, 610–620. https://doi.org/10.1016/j.ympev.2012.07.016
    Castanho, L.M. & Haddad, C.F.B. (2000) New species of Eleutherodactylus (Amphibia: Leptodactylidae) from Guaraqueçaba, Atlantic Forest of Brazil. Copeia, 2000 (3), 777–781. https://doi.org/10.1643/0045-8511(2000)000[0777:NSOEAL]2.0.CO;2
    CEBEA/CFMV [Comissão de Ética, Bioética e Bem-Estar Animal] (2013) Guia brasileiro de boas práticas para eutanásia de animais. Conselho Federal de Medicina Veterinária do Brasil, Brasilia, 22 pp.
    Duellman, W.E. & Lehr, E. (2009) Terrestrial-Breeding Frogs (Strabomantidae) in Peru. Nature und Tier. Verlag GmbH, Münster, 382 pp.
    Edgar, R.C. (2004) MUSCLE: Multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research, 32 (No. 5), 1792–1797. https://doi.org/10.1093/nar/gkh340
    Ferreira, R.B., Beard, K.H. & Crump, M.L. (2016) Breeding guild determines frog distributions in response to edge effects and habitat conversion in the Brazil’s Atlantic Forest. PLoS ONE, 11, e0156781. https://doi.org/10.1371/journal.pone.0156781
    Ferreira, R.B., Mônico, A.T., Silva, E.T., Lirio, F.C.F., Zocca, C., Mageski, M.M., Tonini, J.F.R., Beard, K.H., Duca, C. & Silva-Soares, T. (2019a) Amphibians of Santa Teresa, Brazil: the hotspot further evaluated. Zookeys, 857, 139–162. https://doi.org/10.3897/zookeys.857.30302
    Ferreira, R.B., Lourenço-de-Moraes, R., Zocca, C., Duca, C., Beard, K.H. & Brodie, E.D. (2019b) Antipredator mechanisms of post-metamorphic anurans: a global database and classification system. Behavioral Ecology and Sociobiology, 73, 69. https://doi.org/10.1007/s00265-019-2680-1
    Fitzinger, L.J.F.J. (1826) Neue Classification der Reptilien nach ihren naturlichen Verwandtscaften. J. G. Heubner, Viena, 66 pp.
    Folmer, O., Black, M., Hoeh, W., Lutz, R. & Vrijenhoek, R. (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3, 294–299. https://doi.org/10.1071/ZO9660275
    Frost, D.R. (2021) Amphibian Species of the World: an Online Reference. American Museum of Natural History, New York, New York. Available from: http://research.amnh.org/herpetology/amphibia/index.php (accessed 15 October 2021)
    Garcia, P.C. de A. (1996) Nova espécie de Eleutherodactylus Duméril & Bibron, 1891 (Amphibia: Anura: Leptodactylidae) do Estado de Santa Catarina, Brasil. Biociências, 4, 57–68.
    Gehara, M., Barth, A., Oliveira, E.F. de, Costa, M.A., Haddad, C.F.B. & Vences, M. (2017) Model-based analyses reveal insular population diversification and cryptic frog species in the Ischnocnema parva complex in the Atlantic forest of Brazil. Molecular Phylogenetics and Evolution, 112, 68–78. https://doi.org/10.1016/j.ympev.2017.04.007
    Girard, C. (1853) Descriptions of new species of reptiles, collected by the U.S. Exploring Expedition, under the command of Capt. Charles Wilkes, U.S.N. Second part—including the species of batrachians, exotic to North America. Proceedings of the Academy of Natural Sciences of Philadelphia, 6, 420–424.
    Harrington, B., Gould, T., Jamison, B., Philips, J. & Spike, A. (2004-2005) Inkscape. Available from: http://www.inkscape.org/ (accessed 29 June 2020)
    Hedges, S.B., Duellman, W.E. & Heinicke, M.P. (2008) New World direct-developing frogs (Anura: Terrarana): Molecular phylogeny, classification, biogeography, and conservation. Zootaxa, 1737 (1), 1–182. https://doi.org/10.11646/zootaxa.1737.1.1
    Heyer, W.R. (1984) Variation, systematics, and zoogeography of Eleutherodactylus guentheri and closely related species (Amphibia: Anura: Leptodactylidae). Smithsonian Contributions to Zoology, 402, 1–42. https://doi.org/10.5479/si.00810282.402
    Heyer, W.R. (1985) New species of frogs from Boracéia, São Paulo, Brazil. Proceedings of the Biological Society of Washington, 98, 657–671.
    Heyer, W.R., Rand, A.S., Cruz, C.A.G., Peixoto, O.L. & Nelson, C.E. (1990) Frogs of Boracéia. Arquivos de Zoologia, 31, 235–410.
    Hoogmoed, M.S., Borges, D.M. & Cascon, P. (1994) Three new species of the genus Adelophryne (Amphibia: Anura: Leptodactylidae) from northeastern Brazil, with remarks on the other species of the genus. Zoologische Mededelingen, 68, 271–300.
    Izecksohn, E. (1971) Novo gênero e nova espécie de Brachycephalidae do Estado do Rio de Janeiro, Brasil (Amphibia: Anura). Boletim do Museu Nacional, Nova Série, Zoologia, 280, 1–12.
    Izecksohn, E. (1988) Algumas considerações sôbre o gênero Euparkerella, com a descrição de três novas espécies (Amphibia: Anura: Leptodactylidae). Revista Brasileira de Biologia, 48, 59–74.
    Katoh, K. & Standley, D.M. (2013) MAFFT multiple sequence alignment software version 7: Improvements in performance and usability. Molecular Biology and Evolution, 30, 772–780. https://doi.org/10.1093/molbev/mst010
    Kumar, S., Stecher, G., Li, M., Knyaz, C. & Tamura, K. (2018) MEGA X: Molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution, 35, 1547–1549. https://doi.org/10.1093/molbev/msy096
    Lirio, F.C.F., Ferreira, R.B., Caramaschi, U. & Silva-Soares, T. (2018) Ischnocnema verrucosa (Brazilian Big-headed Frog). Clutch size. Herpetological Review, 49, 302–303.
    Lourenço-de-Moraes, R., Ferreira, R.B., Fouquet, A. & Bastos, R.P. (2014) A new diminutive frog species of Adelophryne (Amphibia: Anura: Eleutherodactylidae) from the Atlantic Forest, southeastern Brazil. Zootaxa, 3846 (3), 348–360. https://doi.org/10.11646/zootaxa.3846.3.2
    Lourenço-de-Moraes, R., Ferreira, R.B., Mira-Mendes, C.V., Zocca, C.Z., Medeiros, T., Ruas, D.S., Rebouças, R., Toledo, L.F., Brodie Jr., E.D. & Solé, M. (2016) Escalated antipredator mechanisms of two neotropical marsupial treefrogs. Herpetological Journal, 26, 237–244.
    Martins, I.A. & Haddad, C.F.B. (2010) A new species of Ischnocnema from highlands of the Atlantic forest, Southeastern Brazil (Terrarana: Brachycephalidae). Zootaxa, 2617 (1), 55–65. https://doi.org/10.11646/zootaxa.2617.1.2
    Miller, M.A., Schwartz, T., Pickett, B.E., He, S., Klem, E.B., Scheuermann, R.H., Passarotti, M., Kaufman, S. & Oleary, M.A. (2015) A RESTful API for access to phylogenetic tools via the CIPRES science gateway. Evolutionary Bioinformatics, 11, 43–48. https://doi.org/10.4137/EBO.S21501
    Miranda-Ribeiro, A. (1920) Algumas considerações sobre Holoaden lüderwaldti e generos correlatos. Revista do Museu Paulista, 12, 319–320.
    Miranda-Ribeiro, A. (1923) Basanitia lactea (Um novo batracchio das collecções do Museu Paulista). Revista do Museu Paulista, 13, 851–852.
    Miranda-Ribeiro, A. (1937) Alguns batrachios novos das collecções do Museu Nacional. O Campo, Rio de Janeiro, 8, 66–69.
    Padial, J.M., Grant, T. & Frost, D.R. (2014) Molecular systematics of terraranas (Anura: Brachycephaloidea) with an assessment of the effects of alignment and optimality criteria. Zootaxa, 3825 (1), 1–132. https://doi.org/10.11646/zootaxa.3825.1.1
    Palumbi, S., Martin, A., Romano, S., McMillan, W.O., Stice, L. & Grabowski, G. (1991) Irish Veterinary Journal The Simple Fool’s Guide to PCR. Privately published document compiled by S. Palumbi, Department of Zoology, University of Hawaii, Honolulu, Hawaii, 43 pp.
    Parker, H.W. (1926) A new brachycephalid frog from Brazil. Annals and Magazine of Natural History Series 9, 18 (104), 201–203. https://doi.org/10.1080/00222932608633496
    Pombal Jr., J.P. (2001) A new species of Brachycephalus (Anura: Brachycephalidae) from Atlantic Rain Forest of southeastern Brazil. Amphibia-Reptilia, 22, 179–185. https://doi.org/10.1163/15685380152030409
    Posada, D. (2008) jModelTest: Phylogenetic model averaging. Molecular Biology and Evolution, 25, 1253–1256. https://doi.org/10.1093/molbev/msn083
    Reinhardt, J.T. & Lütken, C.F. (1862) Bidrag til Kundskab om Brasiliens Padder og Krybdyr. Förste Afdeling: Padderne og Öglerne. Videnskabelige Meddelelser fra Dansk Naturhistorisk Forening i Kjøbenhavn, 2, 143–242.
    Rizzini, C.T. (1979) Tratado de Fitogeografia do Brasil. EDUSP, São Paulo, 123 pp.
    Ronquist, F., Teslenko, M., Van Der Mark, P., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) Mrbayes 3.2: Efficient bayesian phylogenetic inference and model choice across a large model space. Systematic Biology, 61, 539–542. https://doi.org/10.1093/sysbio/sys029
    Sabaj, M.H. (2016) Standard symbolic codes for institutional resource collections in herpetology and ichthyology: an online reference. Version 6.5. American Society of Ichthyologists and Herpetologists, Washington. Available from: http://www.asih.org/ (accessed 20 August 2018)
    Silva-Soares, T., Malagoli, L.R., Cruz, C.A.G. & Caramaschi, U. (2018) On the taxonomy and natural history of the secretive Ischnocnema lactea (Miranda-Ribeiro, 1923) (Anura: Brachycephaloidea: Brachycephalidae). Zootaxa, 4444 (2), 137–153. https://doi.org/10.11646/zootaxa.4444.2.3
    Silva-Soares, T. & Scherrer, P.V. (2013) Amphibians of Parque Estadual do Forno Grande, State of Espírito Santo, Southeastern Brazil: Species composition and conservation. North-Western Journal of Zoology, 9, 113–120.
    Spix, J.B. von (1824) Animalia nova sive Species novae Testudinum et Ranarum quas in itinere per Brasiliam annis MDCCCXVII–MDCCCXX jussu et auspiciis Maximiliani Josephi I. Bavariae Regis. F. S. Hübschmann, München, 150 pp. https://doi.org/10.5962/bhl.title.63182
    Steindachner, F. (1864) Batrachologische Mittheilungen. Verhandlungen des Zoologisch-Botanischen Vereins in Wien, 14, 239–288.
    Targino, M., Costa, P.N. & de Carvalho-e-Silva, S.P. (2009) Two new species of the Ischnocnema lactea species series from Itatiaia highlands, Southeastern Brazil (Amphibia: Anura: Brachycephalidae). South American Journal of Herpetology, 4, 139–150. https://doi.org/10.2994/057.004.0205
    Taucce, P.P., Canedo, C., Parreiras, J.S., Drummond, L.O., Nogueira-Costa, P. & Haddad, C. F. (2018) Molecular phylogeny of Ischnocnema (Anura: Brachycephalidae) with the redefinition of its series and the description of two new species. Molecular Phylogenetics and Evolution, 128, 123–146. https://doi.org/10.1016/j.ympev.2018.06.042

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