Skip to main content Skip to main navigation menu Skip to site footer
Article
Published: 2022-01-18

The phylogenetic status of Limnonectes liui (Yang, 1983) (Anura: Dicroglossidae) based on mitochondrial genes and its taxonomic implications

Key Laboratory of Conserving Wildlife with Small Populations in Yunnan, Southwest Forestry University, Kunming 650224, Yunnan, China
School of Life Science, Henan University, Kaifeng 475004, Henan, China
Key Laboratory of Conserving Wildlife with Small Populations in Yunnan, Southwest Forestry University, Kunming 650224, Yunnan, China
North Carolina Museum of Natural Sciences, 11 West Jones Street, Raleigh, NC 27601, USA
Key Laboratory of Conserving Wildlife with Small Populations in Yunnan, Southwest Forestry University, Kunming 650224, Yunnan, China. Key Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, Southwest University, Chongqing 400715, China
School of Life Science, Henan University, Kaifeng 475004, Henan, China
Amphibia China Laos Myanmar phylogeny synonymy

Abstract

Limnonectes liui (Yang, 1983) is a poorly known frog from Yunnan Province, China, with a confusing taxonomic history. We use mitochondrial (mt) DNA sequence data from newly collected specimens to reconstruct the phylogenetic position of the species. Limnonectes liui was found to be deeply nested within the geographically widespread dicroglossid frog L. limborgi (Sclater, 1892), verifying its placement within the genus Limnonectes but casting doubt on its distinction as a species. Morphological comparisons of the holotype and newly collected specimens of L. liui to specimens of L. limborgi from across its range also corroborated the hypothesis that these two species are conspecific, and we recommend treating L. liui as a junior synonym of L. limborgi. Our study underscores the importance of making comparisons with species found beyond a country’s border when describing new species.

 

References

  1. Borah, M.M., Bordoloi, S., Purkayastha, J., Das, M., Dubois, A. & Ohler, A. (2013) Limnonectes (Taylorana) medogensis (Fei, Ye & Huang, 1997) from Arunachal Pradesh (India), and on the identity of some diminutive ranoid frogs (Anura: Dicroglossidae, Occidozygidae). Herpetozoa, 26 (1/2), 39–48.
    Burland, T.G. (2000) DNASTAR’s Lasergene sequence analysis software. Molecular Biology and Evolution, 132, 71–91. https://doi.org/10.1385/1-59259-192-2:71
    Darriba, D., Taboada, G.L., Doallo, R. & Posada, D. (2012) jModelTest 2: more models, new heuristics and parallel computing. Nature methods, 9 (8), 772–772. https://doi.org/10.1038/nmeth.2109
    Dubois, A. (1987) Miscellanea taxinomica batrachologica (I). Alytes, 5, 7–95.
    Fei, L., Hu, S.Q., Ye, C.Y. & Huang, Y.Z. (2009) Fauna Sinica. Amphibia. Vol. 2 Anura. Science Press, Beijin, 957 pp.
    Fei, L., Ye, C.Y., Huang, Y.Z. & Liu, M.Y. (1999) Atlas of amphibians of China. Henan Science and Technology Press, Zhengzhou, Henan, 432 pp.
    Inger, R.F. & Stuart, B.L. (2010) Systematics of Limnonectes (Taylorana) Dubois. Current Herpetology, 29 (2), 51–68. https://doi.org/10.3105/018.029.0201
    Köhler, G., Vargas, J., Than, N.L., Schell, T., Janke, A., Pauls, S.U. & Thammachoti, P. (2021) A taxonomic revision of the genus Phrynoglossus in Indochina with the description of a new species and comments on the classification within Occidozyginae (Amphibia, Anura, Dicroglossidae). Vertebrate Zoology, 71, 1–26. https://doi.org/10.3897/vz.71.e60312
    Kozlov, A.M., Darriba, D., Flouri, T., Morel, B. & Stamatakis, A. (2019) RAxML-NG: a fast, scalable and user-friendly tool for maximum likelihood phylogenetic inference. Bioinformatics, 35 (21), 4453–4455. https://doi.org/10.1093/bioinformatics/btz305
    Kumar, S., Stecher, G., Li, M., Knyaz, C. & Tamura, K. (2018) MEGA X: molecular evolutionary genetics analysis across computing platforms. Molecular biology and evolution, 35 (6), 1547–1549. https://doi.org/10.1093/molbev/msy096
    Ohler, A. (2007) New synonyms in specific names of frogs (Raninae) from the border regions between China, Laos and Vietnam. Alytes, 25 (1), 55–74. https://doi.org/10.1093/molbev/msy096
    Ronquist, F., Teslenko, M., Van Der Mark, P., Ayres, D.L., Darling, A., Höhna, S. & Huelsenbeck, J.P. (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic biology, 61 (3), 539–542. https://doi.org/10.1093/sysbio/sys029
    Sclater, W.L. (1892) On some specimens of frogs in the Indian Museum, Calcutta, with descriptions of several new species. Proceedings of the zoological Society of London, 1892, 341–348.
    Yang, D. (1983) A new species of the genus Platymantis (Amphibia: Ranidae). Acta Herpetologica Sinica, 2 (2), 53–56.
    Zhao, E.M. & Adler, K. (1993) Herpetology of China. Contributions to herpetology, 10. Society for the Study of Amphibians and Reptiles in cooperation with ChineseSociety for the Study of Amphibians and Reptiles, Oxford, Ohio, 522 pp.

  2.  

  3.  

  4.