Abstract
The Oxynoemacheilus angorae species group is revised and 10 species are recognised, two of which are described herein as new. Oxynoemacheilus anatolicus, O. angorae, O. eregliensis, O. germencicus, O. isauricus, O. nasreddini, O. mediterraneus, and O. theophilii are endemic to west and Central Anatolia. Oxynoemacheilus cinicus and O. mesudae, both from Büyük Menderes River drainage, are synonyms of O. germencicus. As First Revisers, we give priority to Oxynoemacheilus germencicus over O. cinicus. Oxynoemacheilus atili described from Lake Beyşehir basin is a synonym of O. eregliensis. Oxynoemacheilus axylos, new species, from endorheic Lake Tuz basin (Cihanbeyli, Gölyazı, Melendiz, and Samsam) is distinguished by possessing an almost truncate caudal-fin, a stout caudal peduncle, and elevated dorsal and ventral adipose crests. The new species is also distinguished by a minimum K2P sequence divergence of 1.6% in the mtDNA-COI barcode region from O. eregliensis, its closest relative. Oxynoemacheilus eliasi, new species, from the Küçük Menderes and Gediz River drainages, is distinguished by having small, embedded scales on the belly, a narrow suborbital groove in male, an emarginate caudal fin, the caudal peduncle depth 1.3–1.5 times in its length, and the body depth decreasing between the dorsal and caudal-fin bases. The new species is also distinguished by a minimum K2P sequence divergence of 2.7% in the mtDNA-COI barcode region from O. theophilii, its closest relative.
References
Aksu, S., Turan, D. & Emiroğlu, Ö. (2020) Current status freshwater fishes of Nilüfer Stream (North-western Turkey). Biyolojik Çeşitlilik ve Koruma, 13 (3), 351–360. https://doi.org/10.46309/biodicon.2020.836206
Bănărescu, P.M., Nalbant, T.T. & Balik, S. (1978) Süßwasserfische der Türkei. 11. Teil. Die Gattung Orthrias in der Türkei und in Südbulgarien (Pisces, Cobitidae, Noemacheilinae). Mitteilungen aus dem Hamburgischen Zoologischen Museum und Institut, 75, 255–266.
Barbieri, R., Zogaris, S., Kalogianni, E., Stoumboudi, M. Th., Chatzinikolaou, Y., Giakoumi, S., Kapakos, Y., Kommatas, D., Koutsikos, N., Tachos, V., Vardakas, L. & Economou, A.N. (2015) Freshwater fishes and lampreys of Greece: An annotated checklist. Monographs on Marine Sciences No. 8. Hellenic Center for Marine Research, Athens, Greece, 96 pp. [ISBN: 978-960-9798-06-8]
Barlas, M. & Dirican, S. (2004) The fish fauna of the Dipsiz-Çine (Muğla-Aydin) stream. Gazi Üniversitesi Fen Bilimleri Dergisi, 17, 35–48.
Bayari, C.S., Ozyurt, N.N. & Kilani, S. (2009) Radiocarbon age distribution of groundwater in the Konya Closed Basin, central Anatolia, Turkey. Hydrogeology Journal, 17, 347–365. https://doi.org/10.1007/s10040-008-0358-2
Berber, S., Şaşı, H., Topkara, E.T. & Cengiz, Ö. (2011) Apolyont Gölü balık faunasının belirlenmesi. Istanbul Üniversitesi Su Ürünleri Dergisi, 26, 27–55.
Biomatters (2013) Geneious Pro. Available from: http://www.geneious.com (accessed 15 August 2018)
Çiçek, E., Eagderi, S. & Sungur, S. (2019) Oxynoemacheilus phoxinoides (Erk’akan, Nalbant & Özeren, 2007): a junior synonym of Oxynoemacheilus angorae (Steindachner, 1897). FishTaxa, 4 (1), 13–17.
Edgar, R.C. (2004) MUSCLE: Multiple sequence alignment with high accuracy and high throughput. Nucleic acids research, 32, 1792–1797. https://doi.org/10.1093/nar/gkh340.
Ekmekçi, F.G. & Yoğurtçuoğlu, B. (2014) Endemik Bir Balık Türü ile Istilacı Bir Balık Türünün Sakarya Havzası’ndaki Dağılımlarının Belirlenmesi. Hacettepe Üni. Bilimsel Araştırmalar Hızlı Destek Projesi, 013 D05 601 007 Nolu Proje Sonuç Raporu. [unknown pagination, in Turkish]
Erk’akan, F., Nalbant, T.T. & Özeren, S.C. (2007) Seven new species of Barbatula, three new species of Schistura and a new species of Seminemacheilus (Ostariophsi: Balitoridae: Nemachilinae) from Turkey. Journal of Fisheries International, 2, 69–85.
Erk`akan, F. (2012) Two new Oxynoemacheilus (Teleostei: Nemacheilidae) species from Western Turkey. Research Journal of Biological Sciences, 7, 97–101. https://doi.org/10.3923/rjbsci.2012.97.101
Erol, O. (1984) Geomorphology and neotectonics of the pluvial lake basins in the Taurus Belt and South Central Anatolia. In: Tekeli, O. & Göncüoğlu, C. (Eds.), Geology of the Taurus Belt International Symposium, Ankara, 1984, pp. 119–124.
Erol, O. (1986) The relationships between the phases of the development of the Konya-Karapinar obruks and the Pleistocene Tuz Golu and Konya pluvial lakes. In: Karst water Resources (Proc the Ankara-Antalya Symposium – 1985, Gunay, G., Johnson, I. E. (eds.), IAHS Publ. No.161:207–213
Fagundes, P.C., Pereira, E.H.L. & Reis, R.E. (2020) Iterative taxonomic study of Pareiorhaphis hystrix (Siluriformes, Loricariidae) suggests a single, yet phenotypically variable, species in south Brazil. PLoS One, 15, e0237160 https://doi.org/10.1371/journal.pone.0237160
Freyhof, J., Esmaeili, H.R., Sayyadzadeh, G. & Geiger, M. (2014) Review of the crested loaches of the genus Paracobitis from Iran and Iraq with the description of four new species (Teleostei: Nemacheilidae). Ichthyological Exploration of Freshwaters, 25 (1), 11–38.
Freyhof, J., Geiger, M.F., Golzarianpour, K. & Patimar, R. (2016) Sasanidus, a new generic name for Noemacheilus kermanshahensis Bǎnǎrescu & Nalbant, with discussion of Ilamnemacheilus and Schistura (Teleostei; Nemacheilidae). Zootaxa, 4107 (1), 65–80. https://doi.org/10.11646/zootaxa.4107.1.3
Freyhof, J., Kaya, C., Turan, D. & Geiger, M.F. (2019) Review of the Oxynoemacheilus tigris group with the description of two new species from the Euphrates drainage (Teleostei: Nemacheilidae). Zootaxa, 4612 (1), 29–57. https://doi.org/10.11646/zootaxa.4612.1.2
Freyhof, J. & Geiger, M.F. (2021) Oxynoemacheilus shehabi, a new nemacheilid loach from the upper Orontes in southern Syria (Teleostei: Nemacheilidae). Zootaxa, 4908 (4), 571–583. https://doi.org/10.11646/zootaxa.4908.4.9
Freyhof, J., Kaya, C., Epitashvili, G. & Geiger, M.F. (2021) Oxynoemacheilus phasicus, a new nemacheilid loach from the eastern Black Sea basin with some remarks on other Caucasian Oxynoemacheilus (Teleostei: Nemacheilidae). Zootaxa, 4952 (1), 135–151. https://doi.org/10.11646/zootaxa.4952.1.8
Freyhof, J., Kaya, C. & Geiger, M.F. (2022) A practical approach to revise the Oxynoemacheilus bergianus species group (Teleostei: Nemacheilidae). Zootaxa, 5128 (2), 151–194 https://doi.org/10.11646/zootaxa.5128.2.1
Geiger, M.F., Herder, F., Monaghan, M.T., Almada, V., Barbieri, R., Bariche, M., Berrebi, P., Bohlen, J., Casal-Lopez, M., Delmastro, G.B., Denys, G.P.J., Dettai, A., Doadrio, I., Kalogianni, E., Kärst, H., Kottelat, M., Kovačić, M., Laporte, M., Lorenzoni, M., Marčić, Z., Özuluğ, M., Perdices, A., Perea, S., Persat, H., Porcelotti, S., Puzzi, C., Robalo, J., Šanda, R., Schneider, M., Šlechtová, V., Stoumboudi, M., Walter, S. & Freyhof, J. (2014) Spatial heterogeneity in the Mediterranean Biodiversity Hotspot affects barcoding accuracy of its freshwater fishes. Molecular Ecology Resources, 14, 1210–1221. https://doi.org/10.1111/1755-0998.12257
Güçlü, S.S., Küçük, F., Ertan, Ö.O. & Güçlü, Z. (2013) The fish fauna of the Büyük Menderes River (Turkey): taxonomic and zoogeographic features. Turkish Journal of Fisheries and Aquatic Sciences, 13 (4), 685–698. https://doi.org/10.4194/1303-2712-v13_4_14
İlhan, A., Balık, S., (2008). Batı Karadeniz Bölgesi İçsularının Balık Faunası. E.Ü. Su Ürünleri Dergisi, 25 (1), 75–82.
Ivanova, N.V., Zemlak, T.S., Hanner, R.H. & Hebert, P.D.N. (2007) Universal primer cocktails for fish DNA barcoding. Molecular Ecology Notes, 7, 544–548. https://doi.org/10.1111/j.1471-8286.2007.01748.x
Kapli, P., Lutteropp, S., Zhang, J., Kobert, K., Pavlidis, P., Stamatakis, A. & Flouri, T. (2017) Multi-rate Poisson Tree Processes for single-locus species delimitation under Maximum Likelihood and Markov Chain Monte Carlo. Bioinformatics, 33 (11), 1630–1638. https://doi.org/10.1093/bioinformatics/btx025
Kaya, C., Turan, D., Bayçelebi, E., Kalaycı, G. & Freyhof, J. (2020) Oxynoemacheilus cilicicus, a new nemacheilid loach from the Göksu River in southern Anatolia (Teleostei: Nemacheilidae). Zootaxa, 4808 (2), 284–300.
https://doi.org/10.11646/zootaxa.4808.2.3
Kelleci, M., Seçer, B., Çiçek, E. & Sungur, S. (2021) Aksaray İli (Türkiye) İhtiyofaunası. Acta Aquatica Turcica, 17 (2), 279–289. https://doi.org/10.22392/actaquatr.800582
Kimura, M. (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution, 16 (2), 111–120. https://doi.org/10.1007/BF01731581
Kottelat, M. (1990) Indochinese nemacheilines. A revision of nemacheiline loaches (Pisces: Cypriniformes) of Thailand, Burma, Laos, Cambodia and Southern Vietnam. Verlag Dr, Friedrich Pfeil, München, 262 pp.
Kottelat, M. & Freyhof, J. (2007) Handbook of European freshwater fishes. Kottelat, Cornol and Freyhof, Berlin, xiv + 646 pp.
Kottelat, M. (2012) Conspectus Cobitidum: an inventory of the loaches of the world (Teleostei: Cypriniformes: Cobitoidei). Raffles Bulletin of Zoology, 26 (Supplement), 1–199.
Kumar, S., Stecher, G., Li, M., Kınyaz, C. & Tamura, K. (2018) MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms. Molecular Biology and Evolution, 35, 1547–1549. https://doi.org/10.1093/molbev/msy096
Küçük, F., Sarı, H.M., Demir, O. & Gülle, İ. (2009) Review of the ichthyofaunal changes in Lake Eğirdir between 1915 and 2007. Turkish Journal of Zoology, 33, 277–286 https://doi.org/10.3906/zoo-0811-16
Küçük, F., Güçlü, S.S. & Gülle, İ. (2020) Manavgat Irmağı (Antalya) balık faunasının çeyrek asırlık değişimi. Acta Aquatica Turcica, 16 (4), 433–446. https://doi.org/10.22392/actaquatr.693792
Kuzucuoglu, C. (2004) Upper and Late Pleistocene environmental changes in Anatolia, reconstructed from sediment sequences and landforms studies. Proceedings of the International Symposium on Earth System Sciences, Istanbul, Turkey, 8–10 September 2004, 19–27
Malabarba, L.R., Chuctaya, J., Hirschmann, A., Oliveira, E.B. & Thomaz, A.T. (2021) Hidden or unnoticed? Multiple lines of evidence support the recognition of a new species of Pseudocorynopoma (Characidae: Corynopomini). Journal of Fish Biology, 98, 219–236. https://doi.org/10.1111/jfb.14572
Masters, B.C., Fan, V. & Ross, H.A. (2011) Species delimitation—a geneious plugin for the exploration of species boundaries. Molecular Ecology Resources, 11, 154–157. https://doi.org/10.1111/j.1755-0998.2010.02896.x
Saitou, N. & Nei, M. (1987) The Neighbor-Joining Method—a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution, 4, 406–425.
Saylar, Ö., Gül, G., Yılmaz, M. & Gül, A. (2018) Asartepe Baraj Gölü (Ankara) Balık Faunası. Journal of Limnology and Freshwater Fisheries Research, 4 (2), 90–97. https://doi.org/10.17216/limnofish.426094
Steindachner, F. (1897) Bericht über die von Dr. Escherich in der Umgebung von Angora gesammelten Fische und Reptilien. Denkschriften der Kaiserlichen Akademie der Wissenschaften in Wien, Mathematisch-Naturwissenschaftliche Classe, 64, 685–699, pls. 1–4.
Stoumboudi, M.T., Kottelat, M. & Barbieri, R. (2006) The fishes of the inland waters of Lesbos Island, Greece. Ichthyological Exploration of Freshwaters, 17 (2), 129–146.
Sungur, S., (2020) Oxynoemacheilus kosswigi a junior synonym of O. seyhanensis (Teleostei: Nemacheilidae). Iranian Journal of Science and Technology Transaction A-science, 44, 563–573.
https://doi.org/10.1007/s40995-020-00857-9
Swofford, D.L. (2002) PAUP*. Phylogenetic Analysis Using Parsimony (*and Other Methods). Version 4. Sinauer Associates, Sunderland, Massachusetts. [program]
Turan, D., Kaya, C., Kalaycı, G., Baycelebi, E. & Aksu, İ. (2019) Oxynoemacheilus cemali, a new species of stone loach (Teleostei: Nemacheilidae) from the Çoruh River drainage. Journal of Fish Biology, 94 (3), 458–468. https://doi.org/10.1111/jfb.13909
Van Neer, W., Wildekamp, R.H., Küçük, F. & Ünlüsayın, M. (2008) The 1997-1999 surveys of the Anatolian fish fauna and their relevance to the interpretation of trade at Sagalassos. In: Degryse, P. & Waelkens, M. (Eds.), Geo-and Bio-Archeology at Sagalassos and in its Territory. Leuven University Press, Leuven, pp. 299–323. https://doi.org/10.2307/j.ctt9qdxzk.18
Yeates, D.K., Seago, A., Nelson, L., Cameron, S.L., Joseph, L. & Trueman, J.W.H. (2011) Integrative taxonomy, or iterative taxonomy? Systematic Entomology, 36, 209–217. https://doi.org/10.1111/j.1365-3113.2010.00558.x
Yeğen, V., Balık, S., Bilçen, E., Sarı, H.M., Uysal, R., İlhan, A. & Bostan, H. (2007) Afyon ili akarsularında yayılış gösteren balık türleri ve dağılımları [in Turkish]. Türk Sucul Yaşam Dergisi, 3, 419–428.
Yeğen, V., Balık, S., Bilcen, E., Sarı, H.M., Uysal, R. & Yağcı, A. (2008) Fish species of streams in Denizli provinces and their distribution (Turkey). Journal of FisheriesSciences.com, 2, 301–311.
Yoğurtçuoğlu, B. & Freyhof, J. (2018) Aphanius irregularis, a new killifish from south-western Anatolia (Cyprinodontiformes:Aphaniidae) Zootaxa, 4410 (2), 319–330. https://doi.org/10.11646/zootaxa.4410.2.4
Yoğurtçuoğlu, B., Kaya, C., Geiger, M.F. & Freyhof, J. (2020) Revision of the genus Seminemacheilus, with the description of three new species (Teleostei: Nemacheilidae). Zootaxa, 4802 (3), 477–501. https://doi.org/10.11646/zootaxa.4802.3.5
Yoğurtçuoğlu, B., Kaya, C. & Freyhof, J. (2021a) Oxynoemacheilus nasreddini, a new nemacheilid loach from the Central Anatolia (Teleostei: Nemacheilidae). Zootaxa, 4974 (1), 135–150. https://doi.org/10.11646/zootaxa.4974.1.5
Yoğurtçuoğlu, B., Kaya, C., Özuluğ, M. & Freyhof, J. (2021b) Oxynoemacheilus isauricus, a new nemacheilid loach from the Central Anatolia (Teleostei: Nemacheilidae). Zootaxa, 4975 (2), 369–378. https://doi.org/10.11646/zootaxa.4975.2.7
Zhang, J., Kapli, P., Pavlidis, P. & Stamatakis, A. (2013) A general species delimitation method with applications to phylogenetic placements. Bioinformatics, 29 (22), 2869–2876. https://doi.org/10.1093/bioinformatics/btt499