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Article
Published: 2022-09-21

Systematic review of Myotis (Chiroptera, Vespertilionidae) from Chile based on molecular, morphological, and bioacoustic data

Fundação Oswaldo Cruz, Fiocruz Mata Atlântica, 22713-375, Rio de Janeiro, RJ, Brazil
Universidad Santo Tomás, Centro de Investigación e Innovación Para el Cambio Climático, 8370003, Santiago, Chile 3Bioecos E.I.R.L., Las Condes, Santiago, Chile. Programa para la Conservación de los Murciélagos de Chile, Santiago, Chile
Universidad Bernardo O’Higgins, Centro de Investigación en Recursos Naturales y Sustentabilidad, Santiago, Chile
Bioecos E.I.R.L., Las Condes, Santiago, Chile
Smithsonian Institution, National Museum of Natural History, 20560, Washington, DC, USA
Fundação Oswaldo Cruz, Fiocruz Mata Atlântica, 22713-375, Rio de Janeiro, RJ, Brazil
Mammalia Echolocation calls mitochondrial gene multivariate analysis Myotinae Myotis arescens phylogenetics South America

Abstract

Myotis is the most diverse genus of bats in the world, with more than 30 species recognized in the Neotropics. However, many of these species represent cryptic complexes and are evidence of the existence of hidden diversity in several regions. Using an integrative approach based on molecular, morphological, and bioacoustic data, we performed a systematic review of Myotis species from Chile. Phylogenetic inference using cytochrome-b indicated the existence of three monophyletic lineages, and qualitative and quantitative morphological analyses supported these lineages as distinct and morphologically diagnosable taxa. Analysis of discriminant functions using parameters of echolocation calls also indicates the existence of three distinct bioacoustic clusters. Thus, all lines of evidence congruently indicate the existence of three distinct taxa. As a result, we recognize Myotis arescens as a valid and distinct species and define its taxonomic limits from the other species from Chile, Myotis atacamensis and Myotis chiloensis.

 

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