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Type: Article
Published: 2023-03-15
Page range: 93-100
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New Earthworm Record from Division Muzaffarabad, Azad Kashmir, Pakistan Supported by Molecular Markers

1Microbial Biotechnology and Vermitechnology laboratory, Department of Zoology, University of Azad Jammu and Kashmir, King Abdullah Campus, Chattar Kalas, Muzaffarabad, 13100, Pakistan.
1Microbial Biotechnology and Vermitechnology laboratory, Department of Zoology, University of Azad Jammu and Kashmir, King Abdullah Campus, Chattar Kalas, Muzaffarabad, 13100, Pakistan.
2Departamento de Ecoloxia e Bioloxia Animal, Universidade de Vigo, Vigo, E-36310, Spain.
3Department of Computer Sciences and Information Technology, University of Azad Jammu and Kashmir, King Abdullah Campus, Chattar Kalas, Muzaffarabad, 13100, Pakistan.
4Department of Zoology, Government College University Lahore, Pakistan.
Departamento de Biodiversidad, Ecología y Evolución, Facultad de Ciencias Biológicas, Universidad Complutense de Madrid, Madrid, Spain.
Annelida Azad Kashmir earthworms Crassiclitellata Perelia integrative systematics molecular barcoding

Abstract

Earthworm diversity and ecology in Pakistan is poorly known, especially in the region of Azad Jammu & Kashmir. An earthworm community survey assisted by genetic barcoding detected an unidentified species which could constitute a new record for Pakistan. Morphological study revealed its identity as Perelia kaznakovi. Additionally, Bayesian phylogenetic inference based on five mitochondrial and nuclear molecular markers was performed. Results provided a phylogenetic placement of the genus Perelia within Lumbricidae for the first time, indicating a close relationship with Eophila. This approach should be implemented to Perelia arnoldiana and further representatives of the genus in order to understand their biogeography, diversity and evolutionary history.

 

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