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Issue: Vol. 5293 No. 1: 19 May 2023
Type: Article
Published: 2023-05-19
DOI: 10.11646/zootaxa.5293.1.6
Page range: 145-160
Abstract views: 1133
PDF downloaded: 42

Reassessment of species delimitation using nuclear markers in three lentic-breeding salamanders from the Chugoku District of Japan (Amphibia: Caudata: Hynobiidae)

Graduate School of Human and Environmental Studies; Kyoto University; Yoshida Nihonmatsu-cho; Sakyo-ku; Kyoto 606-8501; JAPAN; Graduate School of Global Environmental Studies; Kyoto University; Yoshida Honmachi; Sakyo-ku; Kyoto 606-8501; JAPAN
Graduate School of Human and Environmental Studies; Kyoto University; Yoshida Nihonmatsu-cho; Sakyo-ku; Kyoto 606-8501; JAPAN
Oshigome 4–42–1; Kure; Hiroshima 737–0915; JAPAN
Graduate School of Global Environmental Studies; Kyoto University; Yoshida Honmachi; Sakyo-ku; Kyoto 606-8501; JAPAN
Graduate School of Global Environmental Studies; Kyoto University; Yoshida Honmachi; Sakyo-ku; Kyoto 606-8501; JAPAN
Kuta 435–19–103; Izuhara-Machi; Tsushima; Nagasaki 817-0032; JAPAN
Amphibia Hynobius akiensis Chugoku District Japan taxonomic reexamination specific status

Abstract

Hynobius akiensis sensu lato has recently been split into three species based on short sequence analyses of cyt-b gene of mtDNA and without data of nuclear DNA, and strange sympatric distribution in some areas has been indicated in two species. We analyzed nuclear DNA marker (SNPs) and complete sequence of cyt-b in H. akiensis sensu lato to reassess species delimitation and genetic introgression among species. As a result, we found two lineages with discordant mitochondrial and nuclear DNA in some areas. Of H. akiensis sensu lato, each of the two contains the type locality of two species recently reported (H. sumidai and H. geiyoensis), and the use of these names has been previously advocated. However, their sympatric distribution was rejected based on nuclear DNA data, which we consider is more reliable than mtDNA. We thus clarify geographic boundary of these two species and revise the species delimitations.

 

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