Abstract
We describe Cycloporus pinkipus sp. n., a new polyclad flatworm species from the Adriatic coast of Croatia using live images, histological sections, and a molecular marker. It is the fifteenth described species of Cycloporus Lang, 1884 and the second described congener in the Mediterranean. The genus Cycloporus is characterised by a small oval body, tentacular bumps and the name-giving marginal pores. Cycloporus pinkipus sp. n. has a smooth dorsal surface, which is transparent creamy white with light brown to yellow spots, covered with prominent serial pink spots on the inner rim of the body margin. There is little variation of the genital organs between different species of the genus, therefore we recognise C. pinkipus sp. n. as a new species in particular due to its unique coloration, and a unique partial large nuclear ribosomal subunit (28S) sequence. In recent years the family Euryleptidae Stimpson, 1857, which also contains the genus Cycloporus, was discussed and revised in several molecular studies. In an updated molecular phylogeny of the Polycladida based on partial 18S and 28S rDNA marker genes, C. pinkipus sp. n. was recovered in a clade of many other Cycloporus species within Euryleptidae.
References
- Bahia, J., Padula, V. & Schrödl, M. (2017) Polycldida phylogeny and evolution: integrating evidence from 28S rDNA and morphology. Organisms Diversity & Evolution, 17, 653–678. https://doi.org/10.1007/s13127-017-0327-5 DOI: https://doi.org/10.1007/s13127-017-0327-5
- Bock, S. (1913) Studien über Polycladen. Zoologiska Bidrag från Uppsala, 2, 31–344.
- Cuadrado, D., Rodríguez, J., Moro, L., Grande, C. & Noreña, C. (2021) Polycladida (Platyhelminthes, Rhabditophora) from Cape Verde and related regions of Macaronesia. European Journal of Taxonomy, 736, 1–43. https://doi.org/10.5852/ejt.2021.736.1249 DOI: https://doi.org/10.5852/ejt.2021.736.1249
- Dalyell, J.G. (1853) The Powers of the Creator Displayed in the Creation: Or, Observations on Life Amidst the Various Forms of the Humbler Tribes of Animated Nature. Vol. 1. Van Voorst, London, 359 pp.
- Dittmann, I.L., Cuadrado, D., Aguado, M.T., Noreña, C. & Egger, B. (2019) Polyclad phylogeny persists to be problematic. Organisms Diversity & Evolution, 19 (4), 585–608. https://doi.org/10.1007/s13127-019-00415-1 DOI: https://doi.org/10.1007/s13127-019-00415-1
- Ehrenberg, C.P. (1831) Phytozoa Turbellaria africana et asiatica. In: Hemprich, F. & Ehrenberg, C.P. (Eds.), “Symbolae Physicae.”. Animalia evertebrata exclusis insectis recensuit Dr. CG Ehrenberg. Series Prima cum Tabularum Decade Prima. Officina Academica, Berolini, pp. 1–15.
- Faubel, A. (1984) The Polycladida, Turbellaria. Proposal and establishment of a new system. Part II.The Cotylea. Mitteilungen aus dem Hamburgischen Zoologischen Museum und Institut, 81, 189–259.
- Francotte, C.P. (1897) Recherches sur la maturation, la fécondation et la segmentation chez les polyclades. In: De Lacaze-Duthiers, H. & Pruvot, G. (Eds.), Archives de Zoologie expérimentale et générale: histoire naturelle-histologie-évolution des animaux. Troisième série. Librairie C. Reinwald Schleicher frerés, éditeurs, Paris, pp. 189–298.
- Giard, A. (1873) Contributions à l’histoire naturelle des synascidies. Archives de Zoologie expérimentale et générale, 2, 481–514.
- Grosbusch, A.L., Bertemes, P. & Egger, B. (2019) The adult musculature of two pseudostomid species reveals unique patterns for flatworms (Platyhelminthes, Prolecithophora). Journal of Morphology, 280 (9), 1393–1404. https://doi.org/10.1002/jmor.21039 DOI: https://doi.org/10.1002/jmor.21039
- Hallez, M.P. (1913) Vers polyclades et triclades maricoles. 2me Expédition Antarctique Française (1908-1910). Masson et Cie, Paris, 69 pp.
- Heath, H. & McGregor, E.A. (1912) New polyclads from Monterey Bay, California. Proceedings of the Academy of Natural Sciences of Philadelphia, 64, 455–488.
- ICZN (1999) International Code of Zoological Nomenclature. 4th Edition. The International Trust for Zoological Nomenclature, London, 306 pp.
- Kalyaanamoorthy, S., Minh, B.Q., Wong T.K.F., von Haeseler A. & Jermiin, L.S. (2017) ModelFinder: Fast model selection for accurate phylogenetic estimates. Nature Methods, 14, 587–589. https://doi.org/10.1038/nmeth.4285 DOI: https://doi.org/10.1038/nmeth.4285
- Kato, K. (1934) Polyclad turbellarians from the neighbourhood of the Mitsui Institute of Marine Biology. Japanese Journal of Zoology, 6, 123–138.
- Kato K. (1937) Polyclads collected in Idu, Japan. Japanese Journal of Zoology, 7, 211–232.
- Kato, K. (1944) Polyclads of Japan. The Journal of the Sigenkagaku Kenkyusyo, 1 (3), 257–318.
- Katoh, K. & Standley, D.M. (2013) MAFFT multiple sequence alignment software version 7: Improvements in performance and usability. Molecular Biology and Evolution, 30, 772–780. https://doi.org/10.1093/molbev/mst010 DOI: https://doi.org/10.1093/molbev/mst010
- Lang, A. (1881) Der Bau von Gunda segmentata und die Verwandtschaft der Plathelminthen mit Coelenteraten und Hirudineen. Mittheilungen aus der Zoologischen Station zu Neapel, 3, 187–251.
- Lang, A. (1884) Die Polycladen (Seeplanarien) des Golfes von Neapel und der angrenzenden Meeresabschnitte. Eine Monographie. Fauna und Flora des Golfes von Neapel. W. Engelmann, Leipzig, 688 pp. https://doi.org/10.5962/bhl.title.10545 DOI: https://doi.org/10.5962/bhl.title.10545
- Larsson, K. & Jondelius, U. (2008) Phylogeny of Catenulida and support for Platyhelminthes. Organisms Diversity & Evolution, 8 (5), 378–387. https://doi.org/10.1016/j.ode.2008.09.002 DOI: https://doi.org/10.1016/j.ode.2008.09.002
- Lee, K.-M., Beal, M. & Johnston, E.H. (2006) A new predatory flatworm (Platyhelminthes, Polycladida) from Botany Bay, New South Wales, Australia. Journal of Natural History, 39 (47), 3987–3995. https://doi.org/10.1080/00222930500485263 DOI: https://doi.org/10.1080/00222930500485263
- Litvaitis, M.K., Bolaños, D.M. & Quiroga, S.Y. (2019) Systematic congruence in Polycladida (Platyhelminthes, Rhabditophora): are DNA and morphology telling the same story? Zoological Journal of the Linnean Society, 186 (4), 865–891. https://doi.org/10.1093/zoolinnean/zlz007 DOI: https://doi.org/10.1093/zoolinnean/zlz007
- Marcus, E. (1950) Turbellaria brasileiros (8). Boletim da Faculdade de Filosofia, Ciências e Letras da Universidade de São Paulo, 15, 5–192. https://doi.org/10.11606/issn.2526-4877.bsffclzoologia.1950.125192 DOI: https://doi.org/10.11606/issn.2526-4877.bsffclzoologia.1950.125192
- Marquina, D., Fernández-Álvarez, F.Á. & Noreña, C. (2015) Five new records and one new species of Polycladida (Platyhelminthes) for the Cantabrian coast (North Atlantic) of the Iberian Peninsula. Journal of the Marine Biological Association of the United Kingdom, 95 (2), 311–322. https://doi.org/10.1017/S0025315414001106 DOI: https://doi.org/10.1017/S0025315414001106
- McNab, J.M., Briggs, M.T., Williamson, J.E., Hoffmann, P., Rodriguez, J. & Karuso, P. (2022) Structural characterization and spatial mapping of tetrodotoxins in Australian polyclads. Marine Drugs, 20 (12), 788. https://doi.org/10.3390/md20120788 DOI: https://doi.org/10.3390/md20120788
- Minh, B.Q., Schmidt, H.A., Chernomor, O., Schrempf, D., Woodhams, M.D., von Haeseler, A. & Lanfear, R. (2020) IQ-TREE 2: new models and efficient methods for phylogenetic inference in the genomic era. Molecular Biology and Evolution, 37 (5), 1530–1534. https://doi.org/10.1093/molbev/msaa015 DOI: https://doi.org/10.1093/molbev/msaa015
- Müller, O.F. (1776) Zoologiae Danicae prodromus, seu Animalium Daniae et Norvegiae indigenarum characteres, nomina, et synonyma imprimis popularium. Impensis auctoris, Havniae, 282 pp. DOI: https://doi.org/10.5962/bhl.title.63795
- Newman, L.J. & Cannon, L.R. (2000) A new genus of euryleptid flatworm (Platyhelminthes, Polycladida) from the Indo-Pacific. Journal of Natural History, 34 (2), 191–205. https://doi.org/10.1080/002229300299606 DOI: https://doi.org/10.1080/002229300299606
- Newman, L.J. & Cannon, L.R.G. (2002) The genus Cycloporus (Platyhelminthes: Polycladida) from Australasian waters. Raffles Bulletin of Zoology, 50 (2), 287–299.
- Noreña, C., Marquina, D., Perez, J. & Almon, B. (2014) First records of Cotylea (Polycladida, Platyhelminthes) for the Atlantic coast of the Iberian Peninsula. ZooKeys, 404, 1–22. https://doi.org/10.3897/zookeys.404.7122 DOI: https://doi.org/10.3897/zookeys.404.7122
- Pearse, A.S. (1938) Polyclads of the east coast of North America. Proceedings of the United States National Museum, 86, 67–98. https://doi.org/10.5479/si.00963801.86-3044.67 DOI: https://doi.org/10.5479/si.00963801.86-3044.67
- Pitale, R. & Apte, D. (2021) Further addition to the Indian fauna of Euryleptidae (Polycladida: Cotylea) with description of a new Cycloporus Lang, 1884 and Eurylepta Ehrenberg, 1831. Zootaxa, 5052 (4), 486–500. https://doi.org/10.11646/zootaxa.5052.4.2 DOI: https://doi.org/10.11646/zootaxa.5052.4.2
- Prudhoe, S. (1985) A monograph on Polyclad Turbellaria. British Museum of Natural History and Oxford University Press, London, 259 pp.
- Romeis, B. (1989) Mikroskopische Technik (17 Auflage). Verlag Oldenbourg, München, 697 pp.
- Sars, G.O. (1878) s.n. In: Jensen, O.S. Turbellaria ad litora Norvegiae occidentalia. Turbellarier ved Norges vestkyst. J. W. Eided Bogtrykkeri, Bergen, pp. 1–97.
- Schmarda, L.K. (1859) Neue Turbellarien, Rotatorien und Anneliden, beobachtet und gesammelt auf einer Reise um die Erde 1853 bis 1857. Erste Hälfte. W. Engelmann, Leipzig, 66 pp. https://doi.org/10.5962/bhl.title.85313 DOI: https://doi.org/10.5962/bhl.title.85313
- Schmidtlein, R. (1880) Vergleichende Übersicht über das Erscheinen größerer pelagischer Thiere und Bemerkungen. über Fortpflanzungsverhältnisse einiger Seethiere im Aquarium. Mittheilungen aus der zoologischen Station zu Neapel, 2, 162–175.
- Strand, E.H. (1928) Miscellanea nomenclatoria zoologica et palaeontologica. Archiv für Naturgeschichte, 92A, 30–75.
- Stimpson, W. (1857) Prodromus descriptionis animalium evertebratorum quae in Expeditione ad Oceanum Pacificum Septentrionalem a Republica Federata missa, Johanne Rodgers Duce, observavit et descripsit. Pars I, Turbellaria Dendrocoela. Proceedings of the Academy of Natural Sciences of Philadelphia, 9, 19–31. https://doi.org/10.5962/bhl.title.51447 DOI: https://doi.org/10.5962/bhl.title.51447
- Talavera, G. & Castresana, J. (2007) Improvement of phylogenies after removing divergent and ambiguously aligned blocks from protein sequence alignments. Systematic Biology, 56, 564–577. https://doi.org/10.1080/10635150701472164 DOI: https://doi.org/10.1080/10635150701472164
- Tsunashima, T., Hagiya, M., Yamada, R., Koito, T., Tsuyuki, N., Izawa, S., Kosoba, K., Itoi, S. & Sugita, H. (2017) A molecular framework for the taxonomy and systematics of Japanese marine turbellarian flatworms (Platyhelminthes, Polycladida). Aquatic Biology, 26, 159–167. https://doi.org/10.3354/ab00682 DOI: https://doi.org/10.3354/ab00682
- Tsuyuki, A., Oya, Y. & Kajihara, H. (2020) First record of Stylostomum ellipse (Dalyell, 1853) (Platyhelminthes, Polycladida) from the Pacific Ocean. Check List, 16 (3), 773–779. https://doi.org/10.15560/16.3.773 DOI: https://doi.org/10.15560/16.3.773