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Type: Article
Published: 2023-08-14
Page range: 106-116
Abstract views: 199
PDF downloaded: 19

Divergence time analysis of the Neotropical wasp genus Protopolybia Ducke, 1905 (Vespidae, Polistinae, Epiponini) using a Multilocus Phylogenetic Approach

Universidade Federal do Sul and Sudeste do Pará, Instituto de Estudos do Xingu, R. Constantino Viana, s/n, 68380-000, São Félix do Xingu, PA, Brazil.
Coordenação de Zoologia, Museu Paraense Emílio Goeldi, MCTI, Av. Perimetral s/n, Campus de Pesquisa, 66040-170, Belem, PA, Brazil.
Division of Invertebrate Zoology, American Museum of Natural History.
Universidade Federal do Sul and Sudeste do Pará, Instituto de Estudos do Xingu, R. Constantino Viana, s/n, 68380-000, São Félix do Xingu, PA, Brazil.
Hymenoptera Neotropics Social wasps Molecular Phylogeny Divergence time Protopolybia


The genus Protopolybia Ducke was analyzed using a multilocus phylogenetic approach, which confirmed its monophyly. In contrast with the arrangements derived solely from morphological data, however, this analysis divides the genus into only two major clades. The study also presents divergence times for the origin and diversification of Protopolybia, which are related to possible vicariant events occurring in the Amazon biome.



  1. Arevalo, E., Zhu, Y., Carpenter, J.M. & Strassmann, J.E. (2004) The phylogeny of the social wasp subfamily Polistinae: evidence from microsatellite flanking sequences, mitochondrial COI sequence, and morphological characters. BMC Evolutionary Biology, 4 (8), 1–16.
  2. Bouckaert, R. & Heled, J. (2014) DensiTree 2: Seeing Trees Through the Forest. bioRxiv. [published online]
  3. Carpenter, J.M., Corbara, B. & Dejean, A. (2001) Protopolybia duckei (Du Buysson), a valid species, not a synonym of Protopolybia emortualis (de Saussure). Journal of the New York Entomological Society, 109, 179–181.[0179:PDDBAV]2.0.CO;2
  4. Carpenter, J.M. & Grimaldi, D.A. (1997) Social Wasp in Amber. American Museum Novitates, 3203, 1–10.
  5. Carpenter, J.M. & Wenzel, J.W. (1989) Synonymy of the genera Protopolybia and Pseudochartergus (Hymenoptera, Vespidae, Polistinae). Psyche, 96, 177–186.
  6. Drummond, A.J., Ho, S.Y.W., Phillips, M.J. & Rambaut, A. (2006) Relaxed Phylogenetics and Dating with Confidence. PLoS Biology, 4 (5), e88.
  7. Drummond, A.J., Suchard, M.A., Xie, D. & Rambaut, A. (2012) Bayesian phylogenetics with BEAUti and the BEAST 1.7. Molecular Biology and Evolution, 29 (8), 1969–73.
  8. Ducke, A. (1905) Nouvelles contributions a la connaissance des Vespides sociaux de l’Amerique du Sud. Revue d’Entomologie, 24, 1–24.
  9. Fox, W.J. (1898) Contributions to a knowledge of the Hymenoptera of Brazil, N° 5 Vespidae. Proceedings of the Academy of Natural Sciences of Philadelphia, 1898, 445–460.
  10. Hall, T.A. (1999) BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41, 95–98.
  11. Hoorn, C., Wesselingh, F.P., ter Steege, H., Bermudez, M.A., Mora, A., Sevink, J., Sanmartin, I., Sanchez-Meseguer, A., Anderson, C.L., Figueiredo, J.P., Jaramillo, C., Riff, D., Negri, F.R., Hooghiemstra, H., Lundberg, J., Stadler, T., Sarkinen, T. & Antonelli, A. (2010) Amazonia through time: Andean uplift, climate change, landscape evolution, and biodiversity. Science, 12, 927–931.
  12. Lagomarsino, L.P, Condamine, F.L, Antonelli, A., Mulch, A. & Davis, C.C. (2016) The abiotic and biotic drivers of rapid diversification in Andean bellflowers (Campanulaceae). New Phytologist, 210, 1430–1442.
  13. Lepeletier de Saint-Fargeau, A.L.M. (1836) Histoire naturelle des insects. Hyménoptères. Vol. 1. Roret’s Suites à Buffon, Paris, 547 pp.
  14. Menezes, R.S.T., Brady, S.G., Carvalho, A.F., Del Lama, M.A. & Costa, M.A. (2015) Molecular phylogeny and historical biogeography of the neotropical swarm-founding social wasp genus Synoeca (Hymenoptera: Vespidae). PLoS ONE, 4, e0119151.
  15. Menezes, R.S.T., Lloyd, M.W. & Brady, S.G. (2020) Phylogenomics indicates Amazonia as the major source of Neotropicalswarm-founding social wasp diversity. Proceedings of the Royal Society B, 287, 20200480.
  16. Perrard, A., Nel, A. & Carpenter, J.M. (2014) A new paper wasp from Late Eocene of France (Hymenoptera: Vespidae: Polistinae). Zootaxa, 3753 (6), 542–548.
  17. Piekarski, P.K., Carpenter, J.M., Lemmon, A.R., Lemmon, E.M. & Sharanowski, B.J. (2018) Phylogenomic Evidence Overturns Current Conceptions of Social Evolution in Wasps (Vespidae), Molecular Biology and Evolution, 35 (9), 2097–2109.
  18. Pickett, K.M. & Wenzel, J.W. (2004) Phylogenetic analysis of the New World Polistes (Hymenoptera: Vespidae; Polistinae) using morphology and molecules. Journal of the Kansas Entomological Society, 77 (4), 742–760.
  19. Pickett, K.M., Carpenter, J.M. & Wheeler, W.C. (2006) Systematics of Polistes (Hymenoptera: Vespidae), with a phylogenetic consideration of Hamilton`s haplodiploidy hypothesis. Annales Zoologici Fennici, 43, 390–406.
  20. Pickett, K.M. & Carpenter, J.M. (2010) Simultaneous Analysis and the origin of Eusociality in the Vespidae (Insecta: Hymenoptera). Arthropod Systematics & Phylogeny, 68 (1), 3–33.
  21. Richards, O.W. (1978) The social wasps of the Americas excluding the Vespinae. British Museum (Natural History), London, 580 pp.
  22. Posada, D. & Buckley, T. R. (2004) Model selection and model averaging in phylogenetics: advantages of Akaike Information Criterion and Bayesian approaches over likelihood ratio tests. Systematic Biology, 53,793–808.
  23. Rambaut, A. (Ed.) (2016) FigTree. Version Available from: (accesed 24 July 2023)
  24. Rambaut, A., Suchard, M.A., Xie, W. & Drummond, A.J. (Eds.) (2014) Tracer MCMC trace analysis tool. Version 1.6 [Internet]. Available from: (accessed 24 July 2023)
  25. Ronquist, F., Teslenko, M., van der Mark, P., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology, 61 (3), 539–42. [Epub 22 February 2012, PMID: 22357727, PMCID: PMC3329765]
  26. Santos-Junior, J.N.A., Silveira, O.T. & Carpenter, J.M. (2015) Phylogeny of Protopolybia Ducke, 1905 and taxonomic revision of the Protopolybia exigua species-group (Hymenoptera, Vespidae, Polistinae) with description of four new species. Zootaxa, 3956, (2), 151–182.
  27. Santos-Junior, J.N.A., Silveira, O.T. & Carpenter, J.M. (2018) Taxonomic revision of the Protopolybia sedula species-group (Hymenoptera, Vespidae, Polistinae), with an new identification key to species. Zootaxa, 4403 (1), 87–98.
  28. Santos-Junior, J.N.A., Silveira, O.T. & Carpenter, J.M. (2020) Taxonomic revision of the Protopolybia picteti-emortualis species-group (Richards, 1978), with descriptions of two new species (Hymenoptera: Vespidae, Polistinae). Zootaxa, 4729 (2), 228–248.
  29. Santos-Junior, J.N.A., Silveira, O.T. & Carpenter, J.M. (2020) Taxonomic revision of the P. chartergoides species-group of the genus Protopolybia Ducke, 1905 (Hymenoptera: Vespidae, Polistinae). Zootaxa, 4858 (4), 542–554.
  30. Silveira, O.T. (2004) Protopolybia alicie, a new polistine social wasp from Brazilian Amazonia (Hym., Vespidae, Polistinae, Epiponini). Zootaxa, 598 (1), 1–5.
  31. Silveira, O.T. & Carpenter, J.M. (1995) Protopolybia bituberculata, a new neotropical social wasp (Hymenoptera: Vespidae: Polistinae). Journal of the New York Entomological Society, 103 (1), 48–54.
  32. Silveira, O.T. & Silveira, A.T. (1994) Comparative morphology of skeletal parts of the sting apparatus in Neotropical polistine social wasps (Hymenoptera, Vespidae, Polistinae). Sociobiology, 25 (2), 295–327.
  33. Simon, C., Frati, F., Beckenbach, B., Crespi, B., Liu, H. & Flook, P. (1994) Evolution, Weighting, and Phylogenetic Utility of Mitochondrial Gene Sequences and a Compilation of Conserved Polymerase Chain Reaction Primers. Annals of the Entomological Society of America, 87 (6), 651–701.
  34. Silvestro, D. & Michalak, I. (2012) raxmlGUI: a graphical front-end for RAxML. Organisms Diversity & Evolution, 12, 335–337.
  35. Shneider, H., Bernardi, J.A, Cunha, D.B, Tagliaro, C.H., Vallinoto, M., Ferrari, S. & Sampaio, I. (2011) A molecular analysis of the evolutionary relationships in the Callitrichinae, with emphasis on the position of the dwarf marmoset. Zoologica Scripta, 41 (1), 1–10.
  36. Stamatakis, A. (2014) RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics, 30 (9), 1312–1313.
  37. Swofford, D.L. (2003) PAUP*: Phylogenetic Analysis Using Parsimony (*and Other Methods). Version 4.0b10. Computer software, No Manual. Sinauer Associates, Sunderland, Massachusettes. Available from: (accessed 24 July 2023)
  38. Tanabe, A.S. (2007) kakusan: a computer program to automate the selection of a nucleotide substitution model and the configuration of a mixed model on multilocus data. Molecular Ecology Notes, 7, 962–964.
  39. Thompson, J.D., Higgins, D.G. & Gibson, T.J. (1994) ClustalW: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleics Acids Research, 22, 4673–4680.
  40. Troanskaya, O., Cantor, M., Sherlock, G., Brown, P., Hastie, T., Tibshirani, R., Botstein, D. & Altman, R.B. (2001) Missing value estimation methods for DNA microarrays. Bioinformatics, 17 (6), 520–525.
  41. Vallinoto, M., Sequeira, F., Sodré, D., Bernardi, J., Sampaio, I. & Schneider, H. (2010) Phylogeny and biogeography of the Rhinella marina species complex (Amphibia, Bufonidae) revisited: implications for Neotropical diversification hypotheses. Zoologica Scripta, 39 (2), 128–140.
  42. Varón, A., Vinh, L.S. & Wheeler, W.C. (2004) POY version 4: phylogenetic analysis using dynamic homologies. Cladistics, 26, 72–85.
  43. Wenzel, J.W. (1991) Evolution of nest architecture. In: Ross, K.G. & Matthews, R.W. (Eds.), The social biology of wasps. Cornell University Press, Ithaca, New York, pp. 480–519.
  44. Wenzel, J.W. (1998) A Generic Key to the Nests of Hornets, Yellow jackets, and Paper Wasps Worldwide (Vespidae: Vespinae, Polistinae). American Museum Novitates, 3224, 1–39.
  45. Xiao, J.-H., Jia, J.-G., Murphy, R.W. & Huang, D.-W. (2011) Rapid Evolution of the Mitochondrial Genome in Chalcidoid Wasps (Hymenoptera: Chalcidoidea) Driven by Parasitic Lifestyles. PLoS ONE, 6 (11), e26645.