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Type: Article
Published: 2024-04-02
Page range: 349-378
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The six-spotted spider mite, Eotetranychus sexmaculatus (Riley) (Trombidiformes: Tetranychidae) in Australia, New Zealand, Japan and USA: a revised morphological and molecular-based concept, synonyms, and related species

Queensland Museum; P.O. Box 3300; South Brisbane; Queensland; 4101; Australia
Canadian National Collection of Insects; Arachnids and Nematodes; Ottawa Research and Development Centre; Agriculture and Agri- Food Canada; K.W. Neatby bldg.; 960 Carling Avenue; Ottawa; ON; K1A 0C6; Canada
Canadian National Collection of Insects; Arachnids and Nematodes; Ottawa Research and Development Centre; Agriculture and Agri- Food Canada; K.W. Neatby bldg.; 960 Carling Avenue; Ottawa; ON; K1A 0C6; Canada
Faculty of Economics; Ryutsu Keizai University; Hirahata 120; Ryugasaki; Ibaraki 301-8555; Japan
Acari avocado pest E. queenslandicus spider mites taxonomy

Abstract

Based on morphological and molecular evidence, Eotetranychus asiaticus Ehara is confirmed as a junior synonym of E. sexmaculatus (Riley). Eotetranychus queenslandicus Manson differs to E. sexmaculatus in some morphological characters states, and currently there is insufficient evidence to confirm if it is also a junior synonym. We therefore propose that it remains a valid species until fresh material can be collected from the type host (Codiaeum variegatum (L.) A.Juss.; Euphorbiaceae) in the type location (Freshwater, Queensland, Australia) for molecular analysis and further comparisons. Eotetranychus sexmaculatus is confirmed as present in Australia and New Zealand. Our analyses indicate that specimens identified as E. sexmaculatus from avocado (Persea americana Mill.; Lauraceae) in Western Australia and New Zealand, and specimens of E. asiaticus from the type host (Ficus erecta Thunb.; Moraceae) and type location (Tomigusuku, Okinawa, Japan) are conspecific with specimens of E. sexmaculatus recently collected from the type host (Citrus sp.; Rutaceae) and type location (Florida, USA), with 0–3.6% divergence in a fragment (451 bp) of cytochrome oxidase subunit I (COI), and 0–0.3% in the ribosomal internal transcribed spacer II (ITS2) gene. Herein we redefine E. sexmaculatus, providing a revised diagnosis, notes on leg chaetotaxy, and variation in the shape of the aedeagus—which is a keystone feature critical for determining species boundaries of spider mite species that have males. Our findings also reinforce the potential of the eupathidion on the palptarsus suζ (i.e. the spinneret) for species diagnoses.

References

  1. Banks, N. (1900) The red spiders of the United States (Tetranychus and Stigmaeus). United States Department of Agriculture Division of Entomology Technical Series, 8, 65–77.
  2. Beard, J.J., Beaulieu, F., Knee, W. & Gotoh, T. (2022) Morphological and molecular analyses of the six-spotted spider mite, Eotetranychus sexmaculatus (Riley) (Tetranychidae)—a pest more widespread than anticipated? In: Zhang, Z.-Q., Fan, Q.-H., Heath, A.C.G. & Minor, M.A. (Eds.), Acarological Frontiers: Proceedings of the XVI International Congress of Acarology (1–5 Dec. 2022, Auckland, New Zealand). Zoosymposia 22: 192. https://doi.org/10.11646/zoosymposia.22.1.125
  3. Blommers, L. & Gutierrez, J. (1975) Les tétranyques vivant sur agrumes et avocatiers dans la région de Tamatave (Madagascar-Est) et quelques-uns de leurs prédateurs. Fruits, 30 (3), 191–200.
  4. Bolland, H.R., Gutierrez, J. & Flechtmann, C.H.W. (1998) World Catalogue of the Spider Mite Family (Acari: Tetranychidae). Brill Academic Publishers, Leiden, 392 pp.
  5. Boyle, W.W. (1957) On the mode of dissemination of the two-spotted spider mite, Tetranychus telarius (L.) (Acarina: Tetranychidae). Proceedings of the Hawaiian Entomological Society, 16 (2), 261–268.
  6. Bragard, C., Baptista, P., Chatzivassiliou, E., Di Serio, F., Gonthier, P., Jaques, J.A.M., Justesen, A.F., Magnusson, C.S., Milonas, P., Navas-Cortes, J.A., Parnell, S., Potting, R., Reignault, P.L., Stefani, E., Thulke, H.-H., Van der Werf, W., Civera, A.V., Yuen, J., Zappalà, L., Grégoire, J.-C., Malumphy, C., Kertesz, V., Maiorano, A. & MacLeod, A. (2023) Pest categorisation of Eotetranychus sexmaculatus. European Food Safety Authority Journal, 21, 7898. https://doi.org/10.2903/j.efsa.2023.7898
  7. Collyer, E. (1964) Phytophagous mites and their predators in New Zealand orchards. New Zealand Journal of Agricultural Research, 7 (4), 551–568. https://doi.org/10.1080/00288233.1964.10416383
  8. Davis, J.J. (1968) Studies of Queensland Tetranychidae 7. Records of the genus Eotetranychus (Acarina: Tetranychidae). Journal of the Australian Entomological Society, 7, 127–129. https://doi.org/10.1111/j.1440-6055.1968.tb00717.x
  9. DeLeon, D. (1957) Two new Eotetranychus and a new Oligonychus from southern Florida (Acarina: Tetranychidae). Florida Entomologist, 40 (3), 111–113. https://doi.org/10.2307/3492511
  10. Ehara, S. (1955) On two spider mites parasitic on Japanese Citrus. Annotations Zoologicae Japonenses, 28, 178–182.
  11. Ehara, S. (1966) The tetranychoid mites of Okinawa Island (Acarina: Prostigmata). Journal of the Faculty of Science, Hokkaido University, Series VI (Zoology), 16, 1–22.
  12. Ehara, S. (1969a) Tetranychoid mites of Taiwan. The Journal of the Faculty of Education, Tottori University, Natural Science, 20, 79–103.
  13. Ehara, S. (1969b) Three spider mites of the genus Eotetranychus infesting fruit trees in Japan (Acarina: Tetranychidae). Applied Entomology and Zoology, 4 (1), 16–22. https://doi.org/10.1303/aez.4.16
  14. Ehara, S. (1973) Tetranychoid mites of fruit trees in Japan, with records of their host plants. In: Iwata, S., Nomura, K., Okudai, S., Sugawara, H., Tanaka, M. & Tsuya, T. (Eds.), Acaricide Resistance in Orchard Mites in Japan. Japan Plant Protection Association, Tokyo, pp. 7–10. [in Japanese]
  15. Ehara, S. (1989) Four new species of spider mites (Acarina: Tetranychidae) from Japan. Proceedings of the Japanese Society of Systematic Zoology, 40, 28–38.
  16. Ehara, S. (Ed.) (1993) Plant Mites of Japan in Colors. Zenkoku Noson Kyoiku Kyokai, Tokyo, 298 pp.
  17. Ehara, S. (1996) Systematics of Tetranychoidea. In: Ehara, S. & Shinkaji, N. (Eds.), Principles of Plant Acarology. Zenkoku Noson Kyoiku Kyokai, Tokyo, pp. 39–74. [in Japanese]
  18. Ehara, S. (1999) Revision of the spider mite family Tetranychidae of Japan (Acari, Prostigmata). Species Diversity, 4, 63–141. https://doi.org/10.12782/specdiv.4.63
  19. Ehara, S. & Gotoh, T. (Eds.) (2009) Colored Guide to the Plant Mites of Japan. Zenkoku Noson Kyoiku Kyokai, Tokyo, 349 pp.
  20. Ehara, S. & Gotoh, T. (2006) Description of a new Eotetranychus from Japan with notes on two other species (Acari: Prostigmata: Tetranychidae). International Journal of Acarology, 32 (1), 39–44. https://doi.org/10.1080/01647950608684441
  21. Froggatt, W.W. (1921) Orchard and garden mites. No. 2—Spinning mites (Family Tetranychidae). Agricultural Gazette Sydney, 32, 130–135.
  22. Garrett, L.E. & Haramoto, F.H. (1967) A catalog of Hawaiian Acarina. Proceedings of the Hawaiian Entomological Society, 19 (3), 381–414.
  23. Gotoh, T., Noda, H. & Hong, X.Y. (2003) Wolbachia distribution and cytoplasmic incompatibility based on a survey of 42 spider mite species (Acari: Tetranychidae) in Japan. Heredity, 91, 208–216. https://doi.org/10.1038/sj.hdy.6800329
  24. Grandjean, F. (1940) Les poils et les organes sensitifs portés par les pattes et le palpe chez les Oribates, 2e partie. Bulletin de la Société Zoologique de France, 65, 32–44.
  25. Grandjean, F. (1958) Sur le comportement et la notation des poils accessoires postérieurs aux tarses des nothroïdes et d'autres acariens. Archives de Zoologie Expérimentale et Générale, 96, 277–308.
  26. Guanilo, A.D., de Moraes, G.J., Flechtmann, C.H.W. & Knapp, M. (2012) Phytophagous and fungivorous mites (Acari: Prostigmata, Astigmata) from Peru. International Journal of Acarology, 38 (2), 120–134. https://doi.org/10.1080/01647954.2011.595735
  27. Gupta, Y.N. (1984) On a collection of tetranychoid mites from Tamil Nadu with description of a new species of Aponychus (Acari: Tetranychidae). Bulletin of the Zoological Survery of India, 6 (1–3), 237–245.
  28. Gupta, S.K. & Gupta, Y.N. (1994) A taxonomic review of Indian Tetranychidae (Acari: Prostigmata) with descriptions of new species, re-descriptions of known species and key to genera and species. Memoirs of the Zoological Survey of India, 18 (1), 1–196.
  29. Goff, M.L. (1986) Spider mites (Acari: Tetranychidae) in the Hawaiian Islands. International Journal of Acarology, 12, 43–49. https://doi.org/10.1080/01647958608683437
  30. Hirst, S. (1920) Revision of the English species of red spider (Genera Tetranychus and Oligonychus). Proceedings of the Zoological Society of London, 4, 49–60. https://doi.org/10.1111/j.1469-7998.1920.tb07633.x
  31. Houston, K. & Passlow, T. (1986) Sixspotted mite in Australia. Bulletin of the Entomological Society of Queensland, 14 (6), 100.
  32. Huelsenbeck, J.P. & Ronquist, F. (2001) MRBAYES: Bayesian inference of phylogeny. Bioinformatics, 17, 754–755. https://doi.org/10.1093/bioinformatics/17.8.754
  33. Karuppuchamy, P. & Mohanasundaram, M. (1988) Three new species and records of tetranychid mites (Acari: Tetranychidae) from India. Progress in Acarology, 1, 395–401.
  34. Knee, W., Beaulieu, F., Skevington, J.H., Kelso, S., Cognato, A.L. & Forbes, M.R. (2012) Species boundaries and host range of tortoise mites (Uropodoidea) phoretic on bark beetles (Scolytinae), using morphometric and molecular markers. PLoS ONE, 7(10), e47243. https://doi.org/10.1371/journal.pone.0047243
  35. Lamb, K.P. (1952) A preliminary list of New Zealand Acarina. Transactions of the Royal Society of New Zealand, 79 (3–4), 370–375.
  36. Lo, P.K.C. & Hsia, D.N.T. (1967) Tenuipalpid and tetranychid mites infesting Citrus in Taiwan, and life history study of the Citrus green mite, Schizotetranychus baltazarae Rimando. Bulletin Sun Yat-sen Cultural Foundation, 1, 253–274.
  37. Lo, P.K.C. (1968b) Tetranychoid mites infesting fruit plants in Taiwan. Bulletin Sun Yat-sen Cultural Foundation, 2, 97–137.
  38. Ma, E.P., Yuan, Y.L. & Lin, Y.M. (1979) The spider mites of Hainan Island (Acarina: Tetranychidae). Journal of Jianxi University, 3, 39–49.
  39. Maddison, W.P. & Maddison, D.R. (2021) Mesquite: a modular system for evolutionary analysis. Version 2.75. Available from: http://www.mesquiteproject.org (accessed 14 February 2024)
  40. Manson, D.C.M. (1963) Mites of the families Tetranychidae and Tenuipalpidae associated with Citrus in South East Asia. Acarologia, 5, 351–364.
  41. Manson, D.C.M. (1967) Mites of the families Tenuipalpidae and Tetranychidae intercepted entering New Zealand from overseas. New Zealand Journal of Science, 10, 664–674.
  42. Manson, D.C.M. (1983) List of new mites hostplant records from New Zealand. New Zealand Entomologist, 7, 401–403. https://doi.org/10.1080/00779962.1983.9722429
  43. Mathews, A. (2022) Six-spotted mite—a pest of avocados and grapevines. Available from: https://www.agric.wa.gov.au/avocados/six-spotted-mite-pest-avocados-and-grapevines (accessed 8 February 2023)
  44. Matsuda, T., Morishita, M., Hinomoto, N. & Gotoh, T. (2014) Phylogenetic analysis of the spider mite sub-family Tetranychinae (Acari: Tetranychidae) based on the mitochondrial CO1 gene and the 18S and the 5’ end of the 28S rRNA genes indicates that several genera are polyphyletic. PloS ONE, 9 (10), e108672. https://doi.org/10.1371/journal.pone.0108672
  45. McGregor, E.A. (1919) The red spiders of America and a few European species likely to be introduced. Proceedings of the U.S. National Museum, 56, 641–685. https://doi.org/10.5479/si.00963801.56-2303.641
  46. McGregor, E.A. (1950) Mites of the family Tetranychidae. The American Midland Naturalist, 44, 257–420. https://doi.org/10.2307/2421963
  47. Migeon, A. & Dorkeld, F. (2023) Spider Mites Web: a comprehensive database for the Tetranychidae. Available from: https://www1.montpellier.inrae.fr/CBGP/spmweb (accessed 7 November 2023)
  48. Navajas, M., Lagnel, J., Gutierrez & Boursot, P. (1998) Species-wide homogeneity of nuclear ribosomal ITS2 sequences in the spider mite Tetranychus urticae contrasts with extensive mitochondrial COI polymorphism. Heredity, 80, 742–752. https://doi.org/10.1046/j.1365-2540.1998.00349.x
  49. Nylander, J.A.A. (2004) MrModetest. Version 2. Program Distributed by the Author. Evolutionary Biology Centre, Uppsala University, Uppsala. [program]
  50. Oldham, H.T. & Thorne, F.T. (1947) Six-spotted mite on avocado. Journal of Economic Entomology, 40 (2), 279. https://doi.org/10.1093/jee/40.2.279
  51. Oudemans, A.C. (1931) Acarologische Aanteekeningen. CVII. Entomologische Berichten, 8, 221–236.
  52. Pope, W.T. (1934) Citrus culture in Hawaii. Hawaii Agricultural Experiment Station Bulletin, 71, 1–37. https://doi.org/10.5962/bhl.title.87633
  53. Pritchard, A.E. & Baker, E.W. (1955) A revision of the spider mite family Tetranychidae. Pacific Coast Entomology Society Memoirs, 2, 1–472. https://doi.org/10.5962/bhl.title.150852
  54. Quayle, H.J. (1912) Red spiders and mites of Citrus trees. Bulletin of Agricultural Experiment Station of California, Berkeley, No. 234, 483–530. https://doi.org/10.5962/bhl.title.59342
  55. Riley, C.V. (1890a) The six-spotted spider mite of the orange (Tetranychus 6-maculatus, n. sp.). Insect Life, 2, 225–226.
  56. Riley, C.V. (1890b) The six-spotted mite of the orange (Tetranychus 6-maculatus Riley). Order Acarina; Family Tetranychidae. U.S. Department of Agriculture Report of The Entomologist for the Year 1889, 1890, 340–345.
  57. Rimando, L. (1962) Four new species of spider mites of the genera Eotetranychus and Schizotetranychus (Tetranychihdae, Acarina). Philippine Agriculturist, 45 (10), 535–544.
  58. Ronquist, F. & Huelsenbeck, J.P. (2003) MRBAYES 3: Bayesian phylogenetic inference under mixed models. Bioinformatics, 19, 1572–1574. https://doi.org/10.1093/bioinformatics/btg180
  59. Ronquist, F., Teslenko, M., van der Mark, P., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) MRBAYES 3.2: Efficient Bayesian phylogenetic inference and model selection across a large model space. Systematic Biology, 61, 539–542. https://doi.org/10.1093/sysbio/sys029
  60. Seeman, O.D., Beard, J.J. & Zhang, L. (2017) A new Australian species of Eotetranychus (Acari: Tetranychidae) from buck spinifex Triodia mitchelli (Poaceae), intraspecific variation in Eotetranychus, and the synonymy of Platytetranychus with Eotetranychus. Zootaxa, 4324 (3), 491–517. https://doi.org/10.11646/zootaxa.4324.3.5
  61. Sievers, F., Wilm, A., Dineen, D.G., Gibson, T.J., Karplus, K., Li, W., Lopez, R., McWilliam, H., Remmert, M., Söding, J., Thompson, J.D. & Higgins, D.G. (2011) Fast, scalable generation of high-quality protein multiple sequence alignments using Clustal Omega. Molecular Systems Biology, 7, 539. https://doi.org/10.1038/msb.2011.75
  62. Swofford, D.L. (2003) PAUP*. Phylogenetic analysis using parsimony (*and other methods). Version 4.0b10. Sinauer Associates, Sunderland, Massachusetts. [program]
  63. Tamura, K., Stecher, G. & Kumar, S. (2021) MEGA11: molecular evolutionary genetics analysis version 11. Molecular Biology and Evolution, 38, 3022–3027. https://doi.org/10.1093/molbev/msab120
  64. Tomkins, A.R. (2002) Sustainable management of sixspotted spider mite (Eotetranychus sexmaculatus (Riley)) on avocados. New Zealand Avocado Growers’ Association Annual Research Report, 2, 28–31.
  65. Tuttle, D.M. & Baker, E.W. (1964) The spider mites of Arizona (Acarina: Tetranychidae). Agricultural Experiment Station, University of Arizona, Technical Bulletin, 158, 1–41.
  66. Wang, H.F. (1980) Notes on the genus Eotetranyhcus in China with descriptions of four new species (Acarina: Tetranychidae). Acta Entomologica Sinica, 23, 216–223.