Abstract
A new species, Polycaena eckweileri sp. n., is described from Gansu Province, China. Based on the analysis of the barcode region of the mitochondrial COI gene, the new species belongs to the P. lama Leech, 1893 species group. The new species differs from all the known species of the genus in peculiar characters of the male genitalia. Polycaena eckweileri sp. n. is known to date only from the type locality in Têwo County. The phylogenetic reconstruction of the main lineages of the genus Polycaena based on analysis of a 658 bp region of the COI mitochondrial gene is provided for the first time. Lectotype of Polycaena lua Grum-Grshimailo, 1891 is designated from a series of syntypes deposited in the collection of the Zoological Institute, Russian Academy of Science, St. Petersburg, Russia.
References
- Bartoňová, A., Konvička, M., Marešová, J., Wiemers, M., Ignatev, N., Wahlberg, N., Schmitt, T. & Fric, Z. (2021) Wolbachia affects mitochondrial population structure in two systems of closely related Palaearctic blue butterflies. Scientific Reports, 11, 3019. https://doi.org/10.1038/s41598-021-82433-8
- Espeland, M., Hall, J.P.W., DeVries, P.J., Lees, D.C., Cornwall, M., Hsu, Y.-F., Wu, L.-W., Campbell, D.L., Talavera, G., Vila, R., Salzman, S., Ruehr, S., Lohman, D.J. & Pierce, N.E. (2015) Ancient Neotropical origin and recent recolonisation: Phylogeny, biogeography and diversification of the Riodinidae (Lepidoptera: Papilionoidea). Molecular Phylogenetics and Evolution, 93, 296–306. https://doi.org/10.1016/j.ympev.2015.08.006
- Folmer, O., Black, M., Hoeh, W., Lutz, R. & Vrijenhoek, R.C. (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3, 294– 299.
- Grieshuber, J. & Churkin, S. (2003) Grum-Grshimailo’s journey through China with notes on some Colias taxa. Helios, 4, 224–243, 1 map.
- Grum-Grshimailo, G. (1891) Lepidoptera nova in Asia centrali novissime lecta et descripta. Horae societatis entomologicae Rossicae, 25, 445–465.
- Grum-Grshimailo, G. (1899) Description of a journey in Western China. Vol. II. Across the Bei Shan and the Nan Shan to the Yellow River Valley. Imperial Russian Geographical Society, St. Petersburg, 445 pp. [in Russian]
- Hall, T.A. (2011) BioEdit: An important software for molecular biology. GERF Bulletin of Biosciences, 2 (1), 60–61.
- Hoang, D.T., Chernomor, O., von Haeseler, A., Minh, B.Q. & Vinh, L.S. (2018) UFBoot2: improving the ultrafast bootstrap approximation. Molecular Biology and Evolution, 35 (2), 518–522. https://doi.org/10.1093/molbev/msx281
- Huang, H. & Li, Yu.-F. (2016) Notes on the genus Polycaena Staudinger (Lepidoptera: Riodinidae) with descriptions of two new species from China. Zootaxa, 4105 (6), 575–590. https://doi.org/10.11646/zootaxa.4105.6.4
- Huang, H. (2019) New or little known butterflies from China—3. Neue Entomologische Nachrichten, 77, 203–255.
- Huang, H. (2021) New or little known butterflies from China—4. Atalanta, 52 (3), 338–406.
- ICZN (1999) International Code of Zoological Nomenclature. Fourth edition adopted by the International Union of Biological Sciences. The International Trust for Zoological Nomenclature, London, xxix + 306 pp.
- Ivanova, N.V., de Waard, J.R. & Hebert P.D.N. (2006) An inexpensive, automation-friendly protocol for recovering high-quality DNA. Molecular Ecology Notes, 6, 998–1002. https://doi.org/10.1111/j.1471-8286.2006.01428.x
- Kearse, M., Moir, R., Wilson, A., Stones-Havas, S., Cheung, M., Sturrock, S., Buxton, S, Cooper, A., Markowitz, S., Duran, C., Thierer, T., Ashton, B., Meintjes, P. & Drummond, A. (2012). Geneious Basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics, 28, 1647–1649. https://doi.org/10.1093/bioinformatics/bts199
- Korb, S.K. (2012) The genus Polycaena Staudinger, 1886 (Lepidoptera: Riodinidae) in the Middle Asia: one or two species? Caucasian Entomological Bulletin, 8, 141–143. [in Russian]
- Krupitsky, A.V., Shapoval, N.A., Schepetov, D.M., Ekimova, I.A. & Lukhtanov, V.A. (2022) Phylogeny, species delimitation and biogeography of the endemic Palaearctic tribe Tomarini (Lepidoptera: Lycaenidae). Zoological Journal of the Linnean Society, 196, 630–646. https://doi.org/10.1093/zoolinnean/zlab055
- Krupitsky, A., Shapoval, N. & Shapoval, G. (2023) DNA barcoding of the Palaearctic elfin butterflies (Lepidoptera, Lycaenidae) with a description of four new species from Vietnam. Insects, 14, 352. https://doi.org/10.3390/insects14040352
- Lukhtanov, V.A. & Shapoval, N.A. (2008) Detection of cryptic species in sympatry using population analysis of unlinked genetic markers: A study of the Agrodiaetus kendevani species complex (Lepidoptera: Lycaenidae). Doklady Biological Sciences, 423, 432–436. https://doi.org/10.1134/S0012496608060197
- Lukhtanov, V.A., Dantchenko, A.V., Vishnevskaya, M.S. & Saifitdinova, A.F. (2015a) Detecting cryptic species in sympatry and allopatry: Analysis of hidden diversity in Polyommatus (Agrodiaetus) butterflies (Lepidoptera: Lycaenidae). Biological Journal of the Linnean Society, 116, 468–485. https://doi.org/10.1111/bij.12596
- Lukhtanov, V.A., Shapoval, N.A., Anokhin, B.A., Saifitdinova, A.F. & Kuznetsova, V.G. (2015b) Homoploid hybrid speciation and genome evolution via chromosome sorting. Proceedings of the Royal Society B: Biological Sciences, 282, 20150157. https://doi.org/10.1098/rspb.2015.0157
- Miller, L.D. (1970) Nomenclature of wing veins and cells. Journal of Research on the Lepidoptera, 8 (2), 37–48. https://doi.org/10.5962/p.333547
- Murray, M.G. & Thompson, W.F. (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Research, 8, 4321–4325. https://doi.org/10.1093/nar/8.19.4321
- Nekrutenko, Yu. P. (1998) A catalogue of the type specimens of Riodinidae and Lycaenidae deposited in the collection of Zoologisches Forschungsinstitut und Museum Alexander Koenig (Bonn). Nota Lepidopterologica, 21 (2), 119–148.
- Nordström, F. (1935) Schwedisch-chinesische wissènschaftliche expedition nach den nordwestlichen provinzen Chinas, unter leitung von Dr. Sven Hedin und Prof. Sü Ping-chang. Insekten gesammelt vom schwedischen arzt der expedition Dr. David Hummel 1927–1930. 21. Lepidoptera. 1. Diurna. 2. Sphinges. Arkiv för Zoologi, 27A, 1–37.
- Seraphim, N., Kaminski, L.A., Devries, P.J., Penz, C., Callaghan, C., Wahlberg, N., Silva‐Brandão, K.L. & Freitas, A.V. (2018) Molecular phylogeny and higher systematics of the metalmark butterflies (Lepidoptera: Riodinidae). Systematic Entomology, 43 (2), 407–425. https://doi.org/10.1111/syen.12282
- Shapoval, N.A., Yakovlev, R.V., Kuftina, G.N., Lukhtanov, V.A., Knyazev, S.A., Romanovich, A.E. & Krupitsky, A.V. (2021) Identification of Natural Hybrids between Ahlbergia frivaldszkyi (Lederer, 1853) and Callophrys rubi (Linnaeus, 1758) (Lepidoptera, Lycaenidae) Using Mitochondrial and Nuclear Markers. Insects, 12, 1124. https://doi.org/10.3390/insects12121124
- Staudinger, O. (1886) Centralasiatische Lepidopteren. Stettiner Entomologische Zeitung, 47 (7–9), 225–256.
- Sugiyama, H. (2015) Notes on new and interesting Sino-Himalayan butterflies. Pallarge, 9, 29–53.
- Talavera, G., Lukhtanov, V.A., Rieppel, L., Pierce, N.E. & Vila, R. (2013) In the shadow of phylogenetic uncertainty: the recent diversification of Lysandra butterflies through chromosomal change. Molecular Phylogenetics and Evolution, 69, 469–478. https://doi.org/10.1016/j.ympev.2013.08.004
- Talavera, G., Kaliszewska, Z.A., Heath, A. & Pierce, N.E. (2020) Recent diversification of Chrysoritis butterflies in the South African Cape (Lepidoptera: Lycaenidae). Molecular Phylogenetics and Evolution, 148, 106817. https://doi.org/10.1016/j.ympev.2020.106817
- Tamura, K., Stecher, G. & Kumar, S. (2021) MEGA11: Molecular Evolutionary Genetics Analysis Version 11. Molecular Biology and Evolution. 38 (7), 3022–3027. https://doi.org/10.1093/molbev/msab120
- ten Hagen, W. & Miller, M.A. (2010) Molekulargenetische Untersuchungen der paläarktischen Arten des Genus Callophrys Billberg, 1820 mit Hilfe von mtDNA-COI-Barcodes und taxonomische Überlegungen (Lepidoptera: Lycaenidae). Nachrichten des Entomologischen Vereins Apollo, 30, 177–197.
- Trifinopoulos, J., Nguyen, L.T., von Haeseler, A. & Minh, B.Q. (2016) W-IQ-TREE: a fast online phylogenetic tool for maximum likelihood analysis. Nucleic Acids Research, 44 (W1), W232–W235. https://doi.org/10.1093/nar/gkw256
- Zhang, J., Cong, Q., Shen, J., Opler, P. & Grishin, N. (2021) Genomics-guided refinement of butterfly taxonomy. The Taxonomic Report, 9 (3), 1–54.