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Issue: Vol. 5493 No. 2: 9 Aug. 2024
Type: Article
Published: 2024-08-09
DOI: 10.11646/zootaxa.5493.2.2
Page range: 129-140
Abstract views: 9
PDF downloaded: 0

Morphology and DNA barcodes of four species of Bradysia hilaris group from China (Diptera, Sciaridae)

Department of Forestry Protection; School of Forestry and Biotechnology; Zhejiang A&F University; 666 Wusu Street; Linan; Hangzhou; Zhejiang 311300; P.R. China
Department of Forestry Protection; School of Forestry and Biotechnology; Zhejiang A&F University; 666 Wusu Street; Linan; Hangzhou; Zhejiang 311300; P.R. China
Stückenberg 58; 24226; Heikendorf; Germany
Department of Forestry Protection; School of Forestry and Biotechnology; Zhejiang A&F University; 666 Wusu Street; Linan; Hangzhou; Zhejiang 311300; P.R. China
Department of Forestry Protection; School of Forestry and Biotechnology; Zhejiang A&F University; 666 Wusu Street; Linan; Hangzhou; Zhejiang 311300; P.R. China
Department of Forestry Protection; School of Forestry and Biotechnology; Zhejiang A&F University; 666 Wusu Street; Linan; Hangzhou; Zhejiang 311300; P.R. China
Diptera Tianmu Mountain Bradysia tianmuensis Bradysia curvula Bradysia chikunae new species redescription

Abstract

Four morphologically allied species of the Bradysia hilaris group were studied from China. In a DNA metabarcoding based dipteran diversity study in Zhejiang, eastern China, a hyper-abundant sciarid species was discovered. It was further recognized in this study to be new to science, Bradysia tianmuensis Du & Huang sp. nov., as well as a morphologically similar species, Bradysia curvula Du & Huang sp. nov. Both new species were found to be fairly similar morphologically to the holotype of Bradysia noduspina Yang, Zhang & Yang, 1993 from Guizhou in western China. However, the paratype of B. noduspina appeared to be different from the holotype and determined to be new to science, Bradysia chikunae Du & Huang sp. nov. A phylogenetic tree of all the available 31 COI sequences of the Bradysia hilaris group was provided. Molecular work conducted in the current study also supports Bradysia tianmuensis Du & Huang sp. nov. and Bradysia curvula Du & Huang sp. nov. as new to science thus the four species were described or redescribed accompanied by detailed imagery of habitus and other characters useful for determination.

 

References

  1. Arthofer, W., Gollner, A., Heller, K., Steiner, F.M., Schlick-Steiner, B.C. & Seeber, J. (2021) Contribution to the molecular phylogeny of Sciaridae (Diptera) with special attention to the genera Bradysia and Corynoptera. Zootaxa, 4951 (2), 391–400. https://doi.org/10.11646/zootaxa.4951.2.12
  2. Babytskiy, A.I., Bezsmertna, O.O., Moroz, M.S., Pavliuk, S.D. & Honcharenko, B.V. (2020) The First records of Bradysia species (Diptera, Sciaridae) from Ukraine. Zoodiversity, 54 (4), 329–340. https://doi.org/10.15407/zoo2020.04.329
  3. Broadley, A., Kauschke, E. & Mohrig, W. (2016) Revision of the types of male Sciaridae (Diptera) described from Australia by FAA Skuse. Zootaxa, 4193 (3), 401–450. https://doi.org/10.11646/zootaxa.4193.3.1
  4. Broadley, A., Kauschke, E. & Mohrig, W. (2018). Black fungus gnats (Diptera: Sciaridae) found in association with cultivated plants and mushrooms in Australia, with notes on cosmopolitan pest species and biosecurity interceptions. Zootaxa, 4415 (2), 201–242. https://doi.org/10.11646/zootaxa.4415.2.1
  5. Chen, Z., Wang, L., Zhuo, D., Xu, C. & Liu, X. (2022) A New Cretaceous Dustywing Genus (Neuroptera: Coniopterygidae) with Peculiar Wing Venation. Insects, 13 (7), 654. https://doi.org/10.3390/insects13070654
  6. deWaard, J.R., Ivanova, N.V., Hajibabaei, M. & Hebert, P.D. (2008) Assembling DNA barcodes. In: Environmental genomics. Methods in Molecular Biology, 410, pp. 275–294. https://doi.org/10.1007/978-1-59745-548-0_15
  7. Hayashi, T., Reiter, N., Phillips, R. D., & Peakall, R. (2022) Sexual deception of male Bradysia (Diptera: Sciaridae) by floral odour and morphological cues in Pterostylis (Orchidaceae). Botanical Journal of the Linnean Society, 200 (3), 433–449. https://doi.org/10.1093/botlinnean/boac015
  8. Hebert, P.D., Braukmann, T.W., Prosser, S.W., Ratnasingham, S., DeWaard, J.R., Ivanova, N.V., Janzenm, D.H., Hallwachs, W., Naik, S., Sones, J.E. & Zakharov, E.V. (2018) A Sequel to Sanger: amplicon sequencing that scales. BMC Genomics, 19, 1–14. https://doi.org/10.1186/s12864-018-4611-3
  9. Hebert, P.D., Ratnasingham, S., Zakharov, E.V., Telfer, A.C., Levesque-Beaudin, V., Milton, M.A., Pedersen, S., Jannetta, P. & DeWaard, J.R. (2016) Counting animal species with DNA barcodes: Canadian insects. Philosophical Transactions of the Royal Society B: Biological Sciences, 371 (1702), 20150334. https://doi.org/10.1098/rstb.2015.0333
  10. Hippa, H. & Vilkamaa, P. (1991) The genus Prosciara Frey (Diptera, Sciraridae). Entomologica Fennica, 2, 133–155. https://doi.org/10.33338/ef.83544
  11. Huang, J., Miao, X., Wang, Q., Menzel, F., Tang, P., Yang, D., Wu, H. & Vogler, A.P. (2022) Metabarcoding reveals massive species diversity of Diptera in a subtropical ecosystem. Ecology and Evolution, 12 (1), e8535. https://doi.org/10.1002/ece3.8535
  12. Köhler, A. (2019) The genus Pseudolycoriella Menzel & Mohrig, 1998 (Diptera, Sciaridae) in New Zealand. Zootaxa, 4707 (1), 1–69. https://doi.org/10.11646/zootaxa.4707.1.1
  13. Leng, R., Heller, K., Huang, J., Ye, L. & Wu, H. (2018) DNA barcoding of the genus Dichopygina, with a new species from China (Diptera: Sciaridae). Zoological Systematics, 43 (1), 18–26. https://doi.org/10.11865/zs.201802
  14. Menzel, F. & Dettner, K. (2005) Black Fungus Gnats (Diptera: Sciaridae) collected at cantharidin baits in southern and central Europe. Studia dipterologica, 11, 359–368.
  15. Menzel, F. & Mohrig, W. (2000) Revision der paläarktischen Trauermücken (Diptera: Sciaridae). Studia dipterologica Supplement, 6, 1–761.
  16. Menzel, F., Smith, J.E. & Colauto, N.B. (2003) Bradysia difformis Frey and Bradysia ocellaris (Comstock)—two additional Neotropical species of Black Fungus Gnats (Diptera: Sciaridae) of economic importance: a redescription and review. Annals of the Entomological Society of America, 96 (4), 448–457. https://doi.org/10.1603/0013-8746(2003)096[0448:BDFABO]2.0.CO;2
  17. Miao, X., Huang, J., Menzel, F., Wang, Q., Wei, Q., Lin, X.L. & Wu, H. (2020) Five mitochondrial genomes of black fungus gnats (Sciaridae) and their phylogenetic implications. International Journal of Biological Macromolecules, 150, 200–205. https://doi.org/10.1016/j.ijbiomac.2020.01.271
  18. Mohrig, W., Heller, K., Hippa, H., Vilkamaa, P. & Menzel, F. (2012) Revision of the Black Fungus Gnats (Diptera: Sciaridae) of North America. Studia dipterologica, 19 (1–2), 141–286.
  19. Mohrig, W., Kauschke, E. & Broadley, A. (2018) New black fungus gnats (Diptera: Sciaridae) from Eastern Australia. Zootaxa, 4450 (2), 203–241. https://doi.org/10.11646/zootaxa.4450.2.3
  20. Qi, L., Huang, J., Wu, H. & Wang, Q. (2022) Two new species of Setostylus Matile, 1990 (Diptera: Keroplatidae) from China. Zootaxa, 5165 (3), 443–450. https://doi.org/10.11646/zootaxa.5165.3.9
  21. Roslin, T., Somervuo, P., Pentinsaari, M., Hebert, P.D., Agda, J., Ahlroth, P., Anttonen, P., Aspi, J., Blagoev, G., Blanco, S., Chan, D., Clayhills, T., deWaard, J., deWaard, S., Elliot, T., Elo, R., Haapala, S., Helve, E., Ilmonen, J. & Mutanen, M. (2022) A molecular-based identification resource for the arthropods of Finland. Molecular Ecology Resources, 22 (2), 803–822. https://doi.org/10.1111/1755-0998.13510
  22. Shin, S.G., Jung, S., Heller, K., Menzel, F., Hong, T.K., Shin, J.S., Lee, S.H., Lee, H. & Lee, S. (2015) DNA barcoding of Bradysia (Diptera: Sciaridae) for detection of the immature stages on agricultural crops. Journal of Applied Entomology, 139 (8), 638–645. https://doi.org/10.1111/jen.12198
  23. Shin, S. & Lee, S. (2012) DNA Barcoding of Bradysia (Diptera: Sciaridae) for the Identification of Larval Stages on Agriculture Plants. Annual Meeting and Spring Conference of The Korean Society of Applied Entomology, 2012, pp. 81–81.
  24. Steffan, W.A. (1974) Redescription of Bradysia tritici and Bradysia reynoldsi (Diptera: Sciaridae). Proceedings of the Hawaiian Entomological Society, 21 (3), 467–474.
  25. Vilkamaa, P., Burdíková, N. & Ševčík, J. (2023) The Genus Spinopygina gen. nov. (Diptera, Sciaridae) from Western North America: Preliminary Molecular Phylogeny and Description of Seven New Species. Insects, 14 (2), 173. https://doi.org/10.3390/insects14020173
  26. Wei, Q., Yin, R., Huang, J., Vogler, A.P., Li, Y., Miao, X. & Kardol, P. (2021) The diversity of soil mesofauna declines after bamboo invasion in subtropical China. Science of the Total Environment, 789, 147982. https://doi.org/10.1016/j.scitotenv.2021.147982
  27. Yang, X., Shi, K., Heller, K. & Menzel, F. (2019) Morphology and DNA barcodes of two species of Bradysia Winnertz from China (Diptera, Sciaridae), with the description of Bradysia minorlobus Yang, Shi & Huang sp. nov. Zootaxa, 4612 (1), 85–94. https://doi.org/10.11646/zootaxa.4612.1.5
  28. Yang, C., Zhang, X. & Yang, C. (1993) Classification of Sciaridae in Guizhou Province (Diptera: Nematocera). Entomotaxonomia, 15 (4), 283–318.
  29. Ye, L., Leng, R.X., Huang, J.H., Qu, C. & Wu, H. (2017) Review of three black fungus gnat species (Diptera: Sciaridae) from greenhouses in China. Journal of Asia-Pacific Entomology, 20 (1), 179–184. https://doi.org/10.1016/j.aspen.2016.12.012