Abstract
Isthmian Central America boasts a diverse array of salamanders, with a total of 72 species spanning three genera. Among these, a subset of the subgenus Bolitoglossa (Eladinea), stands out as particularly rare within the Cordillera de Talamanca. Many of its species have been discovered since 2009, with the majority documented from fewer than five specimens. In our endeavor to elucidate the taxonomy of this species group, we conducted fieldwork at Tapantí and the summits of Utyum, Echandi, and Fábregas, aiming to locate specimens. Our efforts have led to the expansion of the distributional range and the elucidation of the known body size of Bolitoglossa bramei, while Bolitoglossa robinsoni underwent redefinition. Furthermore, we describe two additional species herein, including a miniature salamander from Cerro Utyum and another salamander of moderate size from Valle del Silencio. As result of this study, the Bolitoglossa diminuta species group is now recognized to encompass seven species, all confined within the limits of the Cordillera de Talamanca.
References
- AmphibiaWeb (2024) AmphibiaWeb: Information on Amphibian Biology and Conservation. Web application. Berkeley, California. Available from: http://amphibiaweb.org (accessed 16 February 2024)
- Arias, E., Chaves, G. & Parra-Olea, G. (2023) A new species of salamander (Caudata: Plethododntidae: Bolitoglossa) from the subalpine rain páramo of the Cordillera de Talamanca, Costa Rica. Amphibian and Reptile Conservation, 17, 143–160.
- Arias, E., Hertz, A. & Parra-Olea, G. (2019)a Taxonomic assessment of Craugastor podiciferus (Anura: Craugastoridae) in lower Central America with the description of two new species. Amphibian and Reptile Conservation, 13, 173–197.
- Arias, E., Chaves, G., Salazar, S., Salazar-Zúñiga, J.A. & García-Rodríguez, A. (2019)b A new species of dink frog, genus Diasporus (Anura: Eleutherodactylidae), from the Caribbean foothills of the Cordillera de Talamanca, Costa Rica. Zootaxa, 4609 (2), 269–288. https://doi.org/10.11646/zootaxa.4609.2.4
- Bolanos, F. & Wake, D.B. (2009) Two new species of montane web-footed salamanders (Plethodontidae: Bolitoglossa) from the Costa Rica-Panama border region. Zootaxa, 1981 (1), 57–68. https://doi.org/10.11646/zootaxa.1981.1.5
- Bolaños, R., Watson, V. & Tosi, J. (2005) Mapa ecológico de Costa Rica (Zonas de Vida), según el sistema de clasificación de zonas de vida del mundo de L.R. Holdridge, Escala 1:750 000. Centro Científico Tropical, San José, Costa Rica. [map]
- Boza-Oviedo, E., Rovito, S.M., Chaves, G., García-Rodríguez, A., Artavia, L.G., Bolaños, F. & Wake, D.B. (2012) Salamanders from the eastern Cordillera de Talamanca, Costa Rica, with descriptions of five new species (Plethodontidae: Bolitoglossa, Nototriton, and Oedipina) and natural history notes from recent expeditions. Zootaxa, 3309 (1), 36–61. https://doi.org/10.11646/zootaxa.3309.1.2
- Drummond, A.J., Suchard, M.A., Xie, D. & Rambaut, A. (2012) Bayesian phylogenetics with BEAUti and the BEAST 1.7. Molecular Biology and Evolution, 29, 1969–1973. https://doi.org/10.1093/molbev/mss075
- Edgar, R.C. (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research, 32, 1792–1797. https://doi.org/10.1093/nar/gkh340
- Fouquet, A., Gilles, A., Vences, M., Marty, C., Blanc, M. & Gemmell, N.J. (2007) Underestimation of species richness in Neotropical frogs revealed by mtDNA analyses. PLoS ONE, 2 (10), e1109. https://doi.org/10.1371/journal.pone.0001109
- Frost, D.R. (2024) Amphibian Species of the World: an Online Reference. Version 6.0. American Museum of Natural History, New York, New York. Available from: http://research.amnh.org/herpetology/amphibia/index.html (accessed 16 February 2024)
- Hertz, A., Lotzkat, S. & Koehler, G. (2013) A new species of Bolitoglossa (Caudata, Plethodontidae) from the continental divide of western Panama. Zootaxa, 3636 (3), 463–475. https://doi.org/10.11646/zootaxa.3636.3.5
- Holdridge, L.R. (1967) Life Zone Ecology. Revised Edition. Tropical Science Center, San José, Costa Rica. 206 pp.
- Kubicki, B. & Arias, E. (2016) A beautiful new yellow salamander, genus Bolitoglossa (Caudata: Plethodontidae), from the northeastern slopes of the Cordillera de Talamanca, Costa Rica. Zootaxa, 4184 (2), 329–346. https://doi.org/10.11646/zootaxa.4184.2.5
- Kubicki, B., Reyes, A. & Arias, E. (2022) Revised taxonomy and distributions of Costa Rican moss salamanders (Caudata: Plethodontidae: Nototriton), with descriptions of new taxa. Zootaxa, 5194 (4), 451–496. https://doi.org/10.11646/zootaxa.5194.4.1
- Lanfear, R., Calcott, B., Ho, S.Y. & Guindon, S. (2012) PartitionFinder: combined selection of partitioning schemes and substitution models for phylogenetic analyses. Molecular Biology and Evolution, 29, 1695–1701. https://doi.org/10.1093/molbev/mss020
- Lanfear, R., Frandsen, P.B., Wright, A.M., Senfeld, T. & Calcott, B. (2017) PartitionFinder 2: new methods for selecting partitioned models of evolution for molecular and morphological phylogenetic analyses. Molecular Biology and Evolution, 34, 772–773. https://doi.org/10.1093/molbev/msw260
- Miller, M.A., Pfeiffer, W. & Schwartz, T. (2010) Creating the CIPRES Science Gateway for inference of large phylogenetic trees. Proceedings of the Gateway Computing Environments Workshop (GCE), 14 November 2010, 1–8. https://doi.org/10.1109/GCE.2010.5676129
- Moritz, C., Schneider, C.J. & Wake, D.B. (1992) Evolutionary relationships within the Ensatina eschscholtzii complex confirm the ring species interpretation. Systematic Biology, 41, 273–291. https://doi.org/10.1093/sysbio/41.3.273
- Palumbi, S., Martin, A., Romano, S., McMillan, W.O., Stice, L. & Grabowski, G. (1991) The simple fool’s guide to PCR. Version 2.0. Special Publication. Department of Zoology and Kewalo Marine Laboratory, University of Hawaii, Honolulu, Hawaii, 45 pp.
- Parra-Olea, G., García-París, M. & Wake, D.B. (2004) Molecular diversification of salamanders of the tropical American genus Bolitoglossa (Caudata: Plethodontidae) and its evolutionary and biogeographical implications. Biological Journal of the Linnean Society, 81, 325–346. https://doi.org/10.1111/j.1095-8312.2003.00303.x
- Ponce, M., Navarro, D., Morales, R. & Batista, A. (2022) A new salamander of the genus Bolitoglossa (Caudata: Plethodontidae) from of the highlands of western Panama. Zootaxa, 5129 (4), 543–556. https://doi.org/10.11646/zootaxa.5129.4.4
- Robinson, D.C. (1976) A new dwarf salamander of the genus Bolitoglossa (Plethodontidae) from Costa Rica. Proceedings of the Biological Society of Washington, 89, 289–294.
- Rambaut, A., Suchard, M.A., Xie, W. & Drummond, A.J. (2014) Tracer. Version 1.6.1. Available from: http://beast.bio.ed.ac.uk/Tracer (accessed 20 September 2022)
- Ronquist, F., Teslenko, M., Van Der Mark, P., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M. & Huelsenbeck, J.P. (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology, 61, 539–542. https://doi.org/10.1093/sysbio/sys029
- Rovito, S.M. (2017) The geography of speciation in Neotropical salamanders. Herpetologica, 73 (3), 229–241. https://doi.org/10.1655/HERPETOLOGICA-D-16-00077.1
- Rovito, S.M., Parra‐Olea, G., Recuero, E. & Wake, D.B. (2015) Diversification and biogeographical history of Neotropical plethodontid salamanders. Zoological Journal of the Linnean Society, 175, 167–188. https://doi.org/10.1111/zoj.12271
- Sambrook, J. & Russell, D.W. (2006) Purification of nucleic acids by extraction with phenol: chloroform. Cold Spring HarborProtocols, 2006, pdb-prot4455. https://doi.org/10.1101/pdb.prot4455
- Savage, J.M. (1997) A new species of rainfrog of the Eleutherodactylus diastema group from the Alta Talamanca region of Costa Rica. Amphibia-Reptilia, 18, 241–247. https://doi.org/10.1163/156853897X00125
- Sites, J.W. & Marshall, J.C. (2003) Delimiting species: a Renaissance issue in systematic biology. Trends in Ecology & Evolution, 18 (9), 462–470. https://doi.org/10.1016/S0169-5347(03)00184-8
- Sukumaran, J.M. & Holder, M.T. (2010)a SumTrees: Phylogenetic Tree Summarization. Version 4.4.0. Available from: https://github.com/jeetsukumaran/DendroPy (accessed 20 September 2022)
- Sukumaran, J.M. & Holder, M.T. (2010)b DendroPy: A Python library for phylogenetic computing. Bioinformatics, 26, 1569–1571. https://doi.org/10.1093/bioinformatics/btq228
- Tamura, K., Stecher, G., Peterson, D., Filipski, A. & Kumar, S. (2013) MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution, 30, 2725–2729. https://doi.org/10.1093/molbev/mst197
- Vences, M., Thomas, M., Bonett, R.M. & Vieites, D.R. (2005) Deciphering amphibian diversity through DNA barcoding: chances and challenges. Philosophical Transactions of the Royal Society B: Biological Sciences, 360 (1462), 1859–1868. https://doi.org/10.1098/rstb.2005.1717
- Wake, D.B., Savage, J.M. & Hanken, J. (2007) Montane salamanders from the Costa Rica–Panamá border region, with descriptions of two new species of Bolitoglossa. Copeia, 2007, 556–565. https://doi.org/10.1643/0045-8511(2007)2007[556:MSFTCR]2.0.CO;2
- Zwickl, D.J. (2006) Genetic algorithm approaches for the phylogenetic analysis of large biological sequence datasets under the maximum likelihood criterion. Ph.D. Dissertation, The University of Texas, Austin, Texas, 125 pp.