Abstract
Based on morphological and molecular evidences, we describe a new species of Microhyla (Nanohyla) from Deo Ca Mountain in Vietnam. The new species differs from congeners by high genetic divergences in 12S rRNA and 16S rRNA sequences (9.31–11.28 %), surpassing known genetic distances between other recognized species of the subgenus Nanohyla. The new species revealed to be sister to M. nanapollexa from Kon Tum Province, Vietnam. Based on new findings of the latter species, including the first known males, we provide an extended diagnosis of M. nanapollexa including the first record from Kon Tum Province, Vietnam.
References
- Bain, R.H. & Hurley, N.N. (2011) A biogeographic synthesis of the amphibians and reptiles of Indochina. Bulletin of the American Museum of Natural History, 360, 1–138. https://doi.org/10.1206/360.1
- Bain, R.H. & Nguyen, Q.T. (2004) Three new species of narrow-mouth frogs (Genus: Microhyla) from Indochina, with comments on Microhyla annamensis and Microhyla palmipes. Copeia, 3, 507–524. https://doi.org/10.1643/CH-04-020R2
- Felsenstein, J. (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution, 39, 783–791. https://doi.org/10.1111/j.1558-5646.1985.tb00420.x
- Frost, D.R. (2024) Amphibian Species of the World: An Online Reference. Version 6.2. American Museum of Natural History, New York. Available from: http://research.amnh.org/herpetology/amphibia/index.html (accessed 11 April 2024)
- Garg, S., Suyesh, R., Das, A., Jiang, J.P., Wijayathilaka, N., Amarasinghe, A.A.T., Alhadi, F., Vineeth, K.K., Aravind, N.A., Senevirathne, G., Meegaskumbura, N. & Biju, S.D. (2018 "2019") Systematic revision of Microhyla (Microhylidae) frogs of South Asia: a molecular, morphological, and acoustic assessment. Vertebrate Zoology, 69, 1–71. https://doi.org/10.26049/VZ69-1-2019-01
- Glaw, F. & Vences, N. (1997) Anuran eye colouration: definitions, variation, taxonomic implications and possible functions. In: Bohme, W., Bischoff, W. & Ziegler, T. (Eds.), Herpetologia Bonnensis: Proceedings of the 8th Ordinary General Meeting of the Societas Europaea Herpetologica. Bonn, pp. 125–138.
- Gorin, V.A., Trofimets, A.V., Gogoleva, S.I., Le, C.X. & Poyarkov, N.A. (2023) A new species of Nanohyla (Anura: Microhylidae) from the lowland forests of southern Vietnam. Zoological Research, 44, 732–736. https://doi.org/10.24272/j.issn.2095-8137.2023.029
- Gorin, V.A., Scherz, M.D., Korost, D.V. & Poyarkov, N.A. (2021) Consequences of parallel miniaturisation in Microhylinae (Anura, Microhylidae), with the description of a new genus of diminutive South East Asian frogs. Zoosystematics and Evolution, 97, 21–54. https://doi.org/10.3897/zse.97.57968
- Hall, T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. In: Nucleic acids symposium series. Information Retrieval, 95–98.
- Hoang, D.T., Chernomor, O., Von Haeseler, A., Minh, B.Q. & Vinh, L.S. (2018) UFBoot2: Improving the Ultrafast Bootstrap Approximation. Molecular Biology and Evolution, 35 (2), 518–522. https://doi.org/10.1093/molbev/msx281
- Hoang, C.V., Luong, A.M. Nguyen, T.Q., Orlov, N.L., Chen, Y., Wang, B. & Jiang, J.P. (2020) A new species of Microhyla (Amphibia: Anura: Microhylidae) from Langbian Plateau, central Vietnam. Asian Herpetological Research, 11, 161–182. https://doi.org/10.16373/j.cnki.ahr.190060
- IUCN (2001) IUCN Red List categories and criteria, version 3.1, second edition. Gland and Cambridge, 32 pp.
- Mahony, S., Kamei, R.G., Brown, R.M. & Chan, K.O. (2024) Unnecessary splitting of genus-level clades reduces taxonomic stability in amphibians. Vertebrate Zoology, 74, 249–277. https://doi.org/10.3897/vz.74.e114285
- Matsui, N. (2011) Taxonomic revision of one of the Old World's smallest frogs, with description of a new Bornean Microhyla (Amphibia, Microhylidae). Zootaxa, 2814 (1), 33–49. https://doi.org/10.11646/zootaxa.2814.1.3
- Matsui, N., Hamidy, A., Belabut, D.M., Ahmad, N., Panha, S., Suding, A., Khonsue, W., Oh, H.-S., Yong, H.-S., Jiangi, J.-P. & Nishikawa, K. (2011) Systematic relationships of Oriental tiny frogs of the family Microhylidae (Amphibia, Anura) as revealed by mtDNA genealogy. Molecular Phylogenet Evolution, 61 (1), 167–176.
- Matsui, N., Hamidy, A. & Eto, K. (2013) Description of a new species of Microhyla from Bali, Indonesia (Amphibia, Anura). Zootaxa, 3670 (4), 579–590. https://doi.org/10.11646/zootaxa.3670.4.9
- Nguyen, L.T., Schmidt, H.A., Von Haeseler, A. & Minh, B.Q. (2015) IQ-TREE: a fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies. Molecular Biology and Evolution, 32, 268–274. https://doi.org/10.1093/molbev/msu300
- Parker, H.W. (1928) LXIII.-The brevicipitid frogs of the genus Microhyla. Annals and Magazine of Natural History, 2 (11), 473–499. https://doi.org/10.1080/00222932808672911
- Poyarkov, J.N.A., Vassilieva, A.B., Orlov, N.L., Galoyan, E.A., Tran, D., Le, D.T.T., Kretova, V.D. & Geissler, P. (2014) Taxonomy and distribution of narrow-mouth frogs of the genus Microhyla Tschudi, 1838 (Anura: Microhylidae) from Vietnam with descriptions of five new species. Russian Journal of Herpetology, 21 (2), 89–148.
- https://doi.org/10.30906/1026-2296-2014-21-2-89-148
- Rambaut, A., Drummond, A.J., Xie, D., Baele, G. & Suchard, M.A. (2018) Posterior summarisation in Bayesian phylogenetics using Tracer 1.7. Systematic Biology, 67, 901–904. https://doi.org/10.1093/sysbio/syy032
- Ronquist, F., Teslenko, N., Van Der Mark, P., Ayres, D.L., Darling, A., Hӧhna, S., Larget, B., Liu, L., Suchard, N.A. & Huelsenbeck, J.P. (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology, 61, 539–542. https://doi.org/10.1093/sysbio/sys029
- Savage, J.N. (1975) Systematics and distribution of the Mexican and Central American stream frogs related to Eleutherodactylus rugulosus. Copeia, 1975, 254–306. https://doi.org/10.2307/1442883
- Simmons, J.E. (2002) Herpetological collecting and collections management. Revised edition. Society for the Study of Amphibians and Reptiles. Herpetological Circulars, 31, 1–153.
- Simon, C., Frati, F., Beckenbach, A., Crespi, B., Liu, H. & Flook, P. (1994) Evolution, weighting, and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers. Annals of the Entomological Society of America, 87, 651−701. https://doi.org/10.1093/aesa/87.6.651
- Smith, M.A. (1923) Notes on reptiles and batrachians from Siam and Indo-China (no. 2). Journal of the Natural History Society of Siam, 6, 47–53.
- Tamura, K., Stecher, G. & Kumar, S. (2021) MEGA 11: Molecular Evolutionary Genetics Analysis Version 11. Molecular Biology and Evolution, 38 (7), 3022–3027. https://doi.org/10.1093/molbev/msab120
- Tanabe, A.S. (2011) Kakusan 4 and Aminosan: two programs for comparing nonpartitioned, proportional and separate models for combined molecular phylogenetic analyses of multilocus sequence data. Molecular Ecology Resources, 11, 914–921. https://doi.org/10.1111/j.1755-0998.2011.03021.x
- Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F. & Higgins, D.G. (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic acids research, 25, 4876–4882. https://doi.org/10.1093/nar/25.24.4876
- Wu, X., Li Y., Zhang,H., Yan, L. & Wu, X.B. (2014) The complete mitochondrial genome of Microhyla pulchra (Amphidia, Anura, Microhylidae). Mitochondrial DNA, 27 (1), 40–1. https://doi.org/10.3109/19401736.2013.869685
- Zhang, P., Papenfuss, T.J., Wake, M.H., Qu, L. & Wake, D.B. (2008) Phylogeny and biogeography of the family Salamandridae (Amphibia: Caudata) inferred from complete mitochondrial genomes. Molecular Phylogenetics and Evolution, 49 (6), 586–597. https://doi. org/10.1016/j.ympev.2008.08.020