Abstract
The genus Rhoptropus comprises nine recognized species of diurnal geckos endemic to the arid regions of Angola and Namibia. Seven species occur in Angola, including the widespread R. boultoni and the poorly known Angolan endemics R. benguellensis and R. montanus, formerly recognized as subspecies of R. boultoni. While R. benguellensis is relatively widespread in the Angolan Escarpment highlands, R. montanus is geographically restricted to the Huíla Plateau. Recent fieldwork on the Serra da Neve inselberg, a herpetological diversity hotspot in southwestern Angola, revealed the presence of an undescribed species of Rhoptropus at an elevation of approximately 1600 m. An integrative taxonomic approach combining coloration, morphological and molecular data supports the recognition of this population as a distinct taxonomic unit with affinities to R. montanus, which we describe herein as Rhoptropus nivimontanus sp. nov. The discovery of a new species of Rhoptropus endemic to Serra da Neve is discussed in the biogeographic context of the region.
References
- Bachman, S., Moat, J., Hill, A.W., Torre, J. & Scott, B. (2011) Supporting Red List threat assessment withGeoCAT: Geospatial conservation assessment tool. ZooKeys, 150, 117–126. https://doi.org/10.3897/zookeys.150.2109
- Bates, M.F., Lobón-Rovira, J., Stanley, E.L., Branch, W.R. & Vaz Pinto, P. (2023) A new species of green-eyed Cordylus Laurenti, 1768 from the west-central highlands of Angola, and the rediscovery of Cordylus angolensis (Bocage,1895) (Squamata: Cordylidae). Vertebrate Zoology, 73, 599–646. https://doi.org/10.3897/vz.73.e95639
- Bauer, A.M. (1999) Evolutionary scenarios in the Pachydactylus Group geckos of southern Africa: new hypotheses. African Journal of Herpetology, 48 (1–2), 53–62. https://doi.org/10.1080/21564574.1999.9651072
- Bauer, A.M. & Good, D.A. (1996) Phylogenetic systematics of the day geckos, genus Rhoptropus (Reptilia: Gekkonidae), of south-western Africa. Journal of the Zoological Society of London, 238, 635–663. https://doi.org/10.1111/j.1469-7998.1996.tb05420.x
- Bauer, A.M. & Lamb, T. (2005) Phylogenetic Relationships of southern African geckos in the Pachydactylus Group (Squamata: Gekkonidae). African Journal of Herpetology, 54 (2), 105–129. https://doi.org/10.1080/21564574.2005.9635525
- Bauer, A.M., de Silva, A., Greenbaum, E. & Jackman, T.R. (2007) A new species of day gecko from high elevation in Sri Lanka, with a preliminary phylogeny of Sri Lankan Cnemaspis (Reptilia: Squamata: Gekkonidae). Mitteilungen aus dem Museum für Naturkunde in Berlin, Zoologische Reihe, 83, 22–32. https://doi.org/10.1002/mmnz.200600022
- Bauer, A.M., Ceríaco, L.M.P., Marques, M.P. & Becker, F.S. (2023) Highland reptiles of Angola and Namibia. In: Mendelsohn, J., Huntley, B.J. & Vaz Pinto, P. (Eds.), Monograph on endemism in the highlands and escarpments of Angola and Namibia. Namibian Journal of Environment, 8, pp. 259–276.
- Branch, W.R., Vaz Pinto, P., Baptista, N. & Conradie, W. (2019) The Reptiles of Angola: History, Diversity, Endemism and Hotspots. In: Huntley, B., Russo, V., Lages, F. & Ferrand, N. (Eds.), Biodiversity of Angola, Science & Conservation: A Modern Synthesis. Springer, Cham, pp. 283–334. https://doi.org/10.1007/978-3-030-03083-4_13
- Branch, W.R., Schmitz, A., Lobón-Rovira, J., Baptista, N.L., António, T. & Conradie, W. (2021) Rock Island melody: A revision of the Afroedura bogerti Loveridge, 1944 group, with descriptions of four new endemic species from Angola. Zoosystematics and Evolution, 97 (1), 55–82. https://doi.org/10.3897/zse.97.57202
- Brown, R.W. (1956) Composition of scientific words. A manual of methods and a lexicon of materials for the practice of logotechnics. Smithsonian Books, Washington D.C., 882 pp.
- Butler, B.O., Ceríaco, L.M.P., Marques, M.P., Bandeira, S., Júlio, T., Heinicke, M.P. & Bauer, A.M. (2019) Herpetological survey of Huíla Province, Southwest Angola, including first records from Bicuar National Park. Herpetological Review, 50 (2), 225–240.
- Ceríaco, L.M.P., de Sá, S.A.C., Bandeira, S., Valério, H., Stanley, E.L., Kuhn, A.L., Marques, M.P., Vindum, J.V., Blackburn, D.C. & Bauer, A.M. (2016) Herpetological survey of Iona National Park and Namibe Regional Natural Park, with a synoptic list of the amphibians and reptiles of Namibe Province, southwestern Angola. Proceedings of the California Academy of Sciences, 4 (63), 15–61.
- Ceríaco, L.M.P., Marques, M.P., Bandeira, S., Agarwal, I., Stanley, E.L., Bauer, A.M., Heinicke, M.P. & Blackburn, D.C. (2018) A new earless species of Poyntonophrynus (Anura, Bufonidae) from the Serra da Neve Inselberg, Namibe Province, Angola. ZooKeys, 780, 109–136. https://doi.org/10.3897/zookeys.780.25859
- Ceríaco, L.M.P, Marques, M.P., Ilunga, A., Afonso, E., Blackburn, D.C. & Bauer, A.M. (2020) Illustrated type catalogue of the “lost” herpetological collections of Museu do Dundo, Angola. Bulletin of the Museum of Comparative Zoology, 162 (7), 379–440. https://doi.org/10.3099/0027-4100-162.7.379
- Conradie, W., Lobón-Rovira, J., Becker, F.S., Schmitz, A. & Vaz Pinto, P. (2023) Flat gecko (Afroedura) diversity, endemism and speciation in the highlands and escarpments of Angola and Namibia. In: Mendelsohn, J., Huntley, B.J. & Vaz Pinto, P. (Eds.), Monograph on endemism in the highlands and escarpments of Angola and Namibia. Namibian Journal of Environment, 8, pp. 277–281.
- Darko, Y.A., Voss, O. & Uetz, P. (2022) A dictionary of abbreviations used in reptile descriptions. Zootaxa, 5219 (5), 421–432. https://doi.org/10.11646/zootaxa.5219.5.2
- de Queiroz, K. (1999) The general lineage concept of species, species criteria, and the process of speciation. In: Howard, D.J. & Berlocher, S.H. (Eds.), Endless forms: species and speciation. Oxford University Press, New York, New York, pp. 57–75.
- Edgar, R.C. (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research, 32, 1792–1797. https://doi.org/10.1093/nar/gkh340
- FitzSimons, V. (1957) Chapter XI. Reptilia – Serpentes & Sauria. In: Hanström-Perbrinck, B. & Rudebeck, G. (Eds.), South African animal life. Results of the Lund University Expedition in 1950-1951. Almqvist and Wiksell, Stockholm, pp. 385–405.
- Gamble, T., Greenbaum, E., Jackman, T.R., Russell, A.P. & Bauer, A.M. (2012) Repeated origin and loss of adhesive toepads in geckos. PLoS ONE, 7 (6), e39429. https://doi.org/10.1371/journal.pone.0039429
- Growth, J.G. & Barrowclough, G.F. (1999) Basal divergences in birds and the phylogenetic utility of the nuclear RAG-1 gene. Molecular Phylogenetics and Evolution, 12 (2), 115–123. https://doi.org/10.1006/mpev.1998.0603
- Heinicke, M.P., Jackman, T.R. & Bauer, A.M. (2017a) The measure of success: geographic isolation promotes diversification in Pachydactylus geckos. BMC Evolutionary Biology, 17, 9. https://doi.org/10.1186/s12862-016-0846-2
- Heinicke, M.P., Turk, D. & Bauer, A.M. (2017b) Molecular phylogeny reveals strong biogeographic signal and two new species in a Cape Biodiversity Hotspot endemic mini-radiation, the pygmy geckos (Gekkonidae: Goggia). Zootaxa, 4312 (3), 449–470. https://doi.org/10.11646/zootaxa.4312.3.3
- Hellmich, W. (1957) Herpetologische Ergebnisse einer Forschungsreise in Angola. Veröffentlichungen der Zoologischen Staatssammlung, München, 5, 1–92.
- Hewitt, J. (1926) Descriptions of new and little-known lizards and batrachians from South Africa. Annals of the South African Museum, 20 (6), 413–431.
- Huntley, B.J. (2023) Ecology of Angola: Terrestrial Biomes and Ecoregions. Springer, Cham, 460 pp. https://doi.org/10.1007/978-3-031-18923-4
- Kalyaanamoorthy, S., Minh, B.Q., Wong, T.K., Von Haeseler, A. & Jermiin, L.S. (2017) ModelFinder: Fast model selection for accurate phylogenetic estimates. Nature Methods, 14, 587–589.
- Kuhn, A.L. (2016) Systematics of the Namib day geckos (Squamata: Gekkonidae: Rhoptropus). MSc Thesis Villanova University, Villanova, Pennsylvania, vii + 107 pp.
- Lamb, T. & Bauer, A.M. (2001) Mitochondrial phylogeny of Namib day geckos (Rhoptropus) based on cytochrome b and 16S rRNA sequences. Copeia, 2001, 775–780. https://doi.org/10.1643/0045-8511(2001)001[0775:MPONDG]2.0.CO;2
- Lanfear, R., Calcott, B., Ho, S.Y. & Guindon, S. (2012) PartitionFinder: Combined selection of partitioning schemes and substitution models for phylogenetic analyses. Molecular Biology and Evolution, 29, 1695–1701. https://doi.org/10.1093/molbev/mss020
- Laurent, R.F. (1964) Reptiles et Amphibiens de l’Angola. (Troisième contribution). Publicações Culturais da Companhia de Diamantes de Angola, 67, 1–165.
- Lobón-Rovira, J., Vaz Pinto, P., Becker, F.S., Tolley, K.A., Measey, J., Bennet, B., Boon, B., de Sá, S. & Conradie, W. (2022) An updated herpetofaunal species inventory of Iona National Park in southwestern Angola. Check List, 18, 289–321. https://doi.org/10.15560/18.2.289
- Macey, J.R., Larson, A., Ananjeva, N.B., Feng, Z. & Papenfuss, T.J. (1997) Two novel gene orders and the role of light-strand replication in rearrangement of the vertebrate mitochondrial genome. Molecular Biology and Evolution, 14, 91–104. https://doi.org/10.1093/oxfordjournals.molbev.a025706
- Macey, J.R., Schulte, J.A., Larson, A., Ananjeva, N.B., Wang, Y., Pethiyagoda, R., Rastegar-Pouyani, N. & Papenfuss, T.J. (2000) Evaluating trans-Tethys migration: An example using acrodont lizard phylogenies. Systematic Biology, 49, 233–256. https://doi.org/10.1093/sysbio/49.2.233
- Marques, M.P., Ceríaco, L.M.P., Blackburn, D.C. & Bauer, A.M. (2018) Diversity and Distribution of the Amphibians and Terrestrial Reptiles of Angola: Atlas of Historical and Bibliographic Records (1840–2017). Proceedings of the California Academy of Sciences, Series 4, 65 (2), 1–501.
- Marques, M.P., Ceríaco, L.M.P., Stanley, E.L., Bandeira, S.A., Agarwal, I. & Bauer, A.M. (2019) A new species of Girdled Lizard (Squamata: Cordylidae) from the Serra da Neve Inselberg, Namibe Province, southwestern Angola. Zootaxa, 4668 (4), 503–524. https://doi.org/10.11646/zootaxa.4668.4.4
- Marques, M.P., Ceríaco, L.M.P., Buehler, M.D., Bandeira, S.A., Janota, J.M. & Bauer AM (2020) A revision of the Dwarf Geckos, genus Lygodactylus (Squamata: Gekkonidae), from Angola, with the description of three new species. Zootaxa, 4853 (3), 301–352. https://doi.org/10.11646/zootaxa.4853.3.1
- Marques, M.P., Parrinha, D., Tiutenko, A., Lopes-Lima, M., Bauer, A.M. & Ceríaco, L.M.P. (2023) A new species of African legless skink, genus Acontias Cuvier, 1816 “1817” (Squamata: Scincidae) from Serra da Neve inselberg, south-western Angola. African Journal of Herpetology, 72 (2), 145–162. https://doi.org/10.1080/21564574.2023.2246487
- Marques, M.P., Parrinha, D., Lopes-Lima, M., Tiutenko, A., Bauer, A.M. & Ceríaco, L.M.P. (2024a) An Island in a sea of sand: a first checklist of the herpetofauna of the Serra da Neve inselberg, southwestern Angola. ZooKeys, 1201, 167–217. https://doi.org/10.3897/zookeys.1201.120750
- Marques, M.P., Parrinha, D., Lopes-Lima, M., Tiutenko, A., Bauer, A.M. & Ceríaco, L.M.P. (2024b) A treasure trove of endemics: two new species of snake-eyed skinks of the genus Panaspis Cope, 1868 (Squamata, Scincidae) from the Serra da Neve Inselberg, southwestern Angola. Evolutionary Systematics, 8, 167–182. https://doi.org/10.3897/evolsyst.8.121103
- Mendelsohn, J.M. (2019) Landscape changes in Angola. In: Huntley, B.J., Russo, V., Lages, F. & Ferrand, N. (Eds.), Biodiversity of Angola: Science & Conservation, a modern synthesis. Springer, Cham, pp. 123–140. https://doi.org/10.1007/978-3-030-03083-4_8
- Mendelsohn J.M. & Huntley, B.J. (2023) Introducing the highlands and escarpments of Angola and Namibia. In: Mendelsohn, J., Huntley, B.J. & Vaz Pinto, P. (Eds.), Monograph on endemism in the highlands and escarpments of Angola and Namibia. Namibian Journal of Environment, 8, pp. 7–22.
- Mertens, R. (1938) Amphibien und Reptilien aus Angola, gesammelt von W. Schack. Senckenbergiana Biologica, 20 (6), 425–443.
- Minh, B.Q., Nguyen, M.A.T. & Von Haeseler, A. (2013) Ultrafast approximation for phylogenetic bootstrap. Molecular Biology and Evolution, 30, 1188–1195. https://doi.org/10.1093/molbev/mst024
- Moreira, M.O., Fonseca, C. & Rojas, D. (2023) Potential persistence of high-mountain lizards. Ecological Research, 38 (5), 676–689. https://doi.org/10.1111/1440-1703.12410
- Nguyen, L.T., Schmidt, H.A., von Haeseler, A. & Minh, B.Q. (2014) IQ-TREE: a fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies. Molecular Biology and Evolution, 32, 268–274. https://doi.org/10.1093/molbev/msu300
- Palumbi, S.R. (1996) Nucleic acids II: the polymerase chain reaction. In: Hillis, D.M., Moritz, C. & Mable, B.K. (Eds), Molecular Systematics. 2nd Edition. Sinauer Associates, Sunderland, MA, pp. 205–247.
- Peters, W.C.H. (1869) Eine Mittheilung über neue Gattungen und Arten von Eidechsen. Monatsberichte der Königlichen Preussische Akademie des Wissenschaften zu Berlin, pp. 57–66, 1 pl.
- Schmidt, K.P. (1933) The reptiles of the Pulitzer Angola Expedition. Annals of the Carnegie Museum, 22(1), 1–15. https://doi.org/10.5962/p.214561
- Sinervo, B., Méndez-de-la-Cruz, F., Miles, D.B., Heulin, B., Bastiaans, E., Villagrán-Santa Cruz, M., Lara-Resendiz, R., Martínez-Méndez, N., Calderón-Espinosa, M.L., Meza-Lázaro, R.N., Gadsden, H., Avila, L.J., Morando, M., De la Riva, I.J., Sepulveda, P.V., Rocha, C.F.D., Ibargüengoytía, N., Puntriano, C.A., Massot, M., Lepetz, V., Oksanen, T.A., Chapple, D.G., Bauer, A.M., Branch, W.R., Clobert, J. & Sites Jr., J.W. (2010) Erosion of lizard diversity by climate change and altered thermal niches. Science, 328 (5980), 894–899. https://doi.org/10.1126/science.1184695
- Tamura, K., Stecher, G. & Kumar, S. (2021) MEGA11: Molecular Evolutionary Genetics Analysis version 11. Molecular Biology and Evolution, 38, 3022–3027. https://doi.org/10.1093/molbev/msab120
- Uetz, P., Freed, P., Aguilar, R., Reyes, F., Kudera, J. & Hošek, J. (Eds.) (2023) The Reptile Database. Available from: http://www.reptile-database.org (accessed 9 June 2024)
- Vaz Pinto, P., Russo, V. & Veríssimo, L. (2023) The highlands in Angolan conservation areas. In: Mendelsohn, J., Huntley, B.J. & Vaz Pinto, P. (Eds.), Monograph on endemism in the highlands and escarpments of Angola and Namibia. Namibian Journal of Environment, 8, 53–62.