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Issue: Vol. 5660 No. 1: 8 Jul. 2025
Type: Correspondence
Published: 2025-07-08
DOI: 10.11646/zootaxa.5660.1.11
Page range: 139-146
Abstract views: 33
PDF downloaded: 1

Larval morphology and DNA barcode of Synorthocladius bifidus Liu & Wang, 2005 (Diptera: Chironomidae)

Engineering Research Center of Environmental DNA and Ecological Water Health Assessment; Shanghai Ocean University; Shanghai 201306; China; Shanghai Universities Key Laboratory of Marine Animal Taxonomy and Evolution; Shanghai Ocean University; Shanghai 201306; China
Engineering Research Center of Environmental DNA and Ecological Water Health Assessment; Shanghai Ocean University; Shanghai 201306; China; Shanghai Universities Key Laboratory of Marine Animal Taxonomy and Evolution; Shanghai Ocean University; Shanghai 201306; China
Engineering Research Center of Environmental DNA and Ecological Water Health Assessment; Shanghai Ocean University; Shanghai 201306; China; Key laboratory of Exploration and Utilization of Aquatic Genetic Resources of the Ministry of Education; Shanghai Ocean University; Shanghai 201306; China
Engineering Research Center of Environmental DNA and Ecological Water Health Assessment; Shanghai Ocean University; Shanghai 201306; China; Shanghai Universities Key Laboratory of Marine Animal Taxonomy and Evolution; Shanghai Ocean University; Shanghai 201306; China
Diptera Chironomidae Synorthocladius bifidus

References

  1. Andersen, T., Sæther, O., Cranston, P. & Epler, J. (2013) The larvae of Orthocladiinae (Diptera: Chironomidae) of the Holarctic region—Keys and diagnoses. Insect Systematics & Evolution, 66, 189–385.
  2. Ashe, P. & O’Connor, J. (2012) A World Catalogue of Chironomidae (Diptera). Part 2. Orthocladiinae. Irish Biogeographical Society and National Museum of Ireland, Dublin, xvi + 968 pp.
  3. Cranston, P.S. (1979) The biosystematics of British aquatic larval orthocladiinae (Diptera: Chironomidae). Ph.D. Thesis, University of London, London, 359 pp., 79 pls.
  4. deWaard, J.R., Ivanova, N.V., Hajibabaei, M. & Hebert, P.D.N. (2008) Assembling DNA barcodes: analytical protocols. In: Cristofre, M. (Ed.), Methods in Molecular Biology: Environmental Genetics. Humana Press Inc., Ottawa, pp. 275–293. https://doi.org/10.1007/978-1-59745-548-0_15
  5. Edgar, R.C. (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research, 32, 1792–1797. https://doi.org/10.1093/nar/gkh340
  6. Ekrem, T., Stur, E., Orton, M.G. & Adamowicz, S.J. (2018) DNA barcode data reveal biogeographic trends in Arctic non-biting midges. Genome, 61, 787–796. https://doi.org/10.1139/gen-2018-0100
  7. Han, W., Wei, J., Lin, X. & Tang, H. (2020) The Afro-Oriental Genus Yaeprimus Sasa et Suzuki (Diptera: Chironomidae: Chironomini): Phylogeny, New Species and Expanded Diagnoses. Diversity, 12, 31. https://doi.org/10.3390/d12010031
  8. Lencioni, V. & Moubayed, J. (2021) Synorthocladius federicoi sp. nov., a new species occurring in the middle basin of the Adige River, northern Italy (Diptera, Chironomidae, Orthocladiinae). ZooKeys, 1057, 105–116. https://doi.org/10.3897/zookeys.1057.68175
  9. Li, S.-Y., Yao, Y., Sun, L., Ling, H.-N., Jin, W.-D. & Lin, X.-L. (2023) DNA barcodes and morphology reveal new species within the Rheotanytarsus guineensis species group from China (Diptera: Chironomidae). Archives of Insect Biochemistry and Physiology, 114, e22060. https://doi.org/10.1002/arch.22060
  10. Lin, X.-L., Stur, E. & Ekrem, T. (2018) DNA barcodes and morphology reveal unrecognized species of Chironomidae (Diptera). Insect Systematics & Evolution, 49, 329–398. https://doi.org/10.1163/1876312X-00002172
  11. Lin, X.-L., Yu, H.-J., Wang, X.-H., Bu, W.-J., Wang, X.-H., Yan, C.-C. & Liu, W.-B. (2021a) New or little-known Boreoheptagyia (Diptera: Chironomidae) in China inferred from morpholgy and DNA barcodes. ZooKeys, 1040, 187–200. https://doi.org/10.3897/zookeys.1040.66527
  12. Lin, X.-L., Jiang, K., Liu, W.-B., Liu, W., Bu, W.-J., Wang, X.-H. & Mo, L. (2021b) Toward a global DNA barcode reference library of the intolerant nonbiting midge genus Rheocricotopus Brundin, 1956. Ecology and Evolution, 11, 12161–12172. https://doi.org/10.1002/ece3.7979
  13. Lin, X.-L., Mo, L., Bu, W.-J. & Wang, X.-H. (2021c) The first comprehensive DNA barcode reference library of Chinese Tanytarsus (Diptera: Chironomidae) for environmental DNA metabarcoding. Diversity and Distributions, 27, 1932–1941. https://doi.org/10.1111/ddi.13209
  14. Liu, Y.D. & Wang, X.H. (2005) Synorthocladius Thienemann from China, with a review of the genus (Diptera: Chironomidae: Orthocladiinae). Zootaxa, 1057 (1), 51–60. https://doi.org/10.11646/zootaxa.1057.1.3
  15. Ratnasingham, S. & Hebert, P.D.N. (2007) BOLD: The Barcode of Life Data System (www.barcodinglife.org). Molecular Ecology Notes, 7, 355–364. https://doi.org/10.1111/j.1471-8286.2006.01678.x
  16. Sæther, O.A. (1969) Some Nearctic Podonominae, Diamesinae, and Orthocladiinae (Diptera: Chironomidae). Bulletin of the Fisheries Research Board of Canada, 170, 1–154.
  17. Sæther, O.A. (1980) Glossary of chironomid morphology terminology (Diptera: Chironomidae). Entomologica Scandinavica Supplement, 14, 1–51.
  18. Song, C., Lin, X.-L., Wang, Q. & Wang, X.-H. (2018) DNA barcodes successfully delimit morphospecies in a superdiverse insect genus. Zoologica Scripta, 47, 311–324. https://doi.org/10.1111/zsc.12284
  19. Tamura, K., Stecher, G. & Kumar, S. (2021) MEGA11: molecular evolutionary genetics analysis version 11. Molecular Biology and Evolution, 38, 3022–3027. https://doi.org/10.1093/molbev/msab120
  20. Thienemann, A. (1935) Chironomiden-Metamorphosen. 10:” Orthocladius-Dactylocladius”(Dipt.). Stettiner Entomologische Zeitung, 96, 201–224.
  21. Wang, H., Xu, H.-F., Li, C.-H., Zhang, H.-X., Zhang, Y.-P., Sun, B.-J. & Lin, X.-L. (2024) Towards a Comprehensive DNA Barcode Library of Stenochironomus Kieffer, 1919 (Diptera: Chironomidae) from China. Diversity, 16, 257. https://doi.org/10.3390/d16050257
  22. Yao, Y., Chen, J.-Y., Gong, X.-L., Li, C.-H., Liu, Z. & Lin, X.-L. (2025) Species Delimitation and Cryptic Diversity in Rheotanytarsus Thienemann & Bause, 1913 (Diptera: Chironomidae) Based on DNA Barcoding. Insects, 16, 370. https://doi.org/10.3390/insects16040370

How to Cite

Wang, R.-H., Zhang, Z.-C., Feng, H.-Y. & Lin, X.-L. (2025) Larval morphology and DNA barcode of Synorthocladius bifidus Liu & Wang, 2005 (Diptera: Chironomidae). Zootaxa, 5660 (1), 139–146. https://doi.org/10.11646/zootaxa.5660.1.11