Abstract
Based on integrative taxonomic analyses, we describe a new species of Acanthosaura from Krong Bong forest, Dak Lak Province, the Central Highlands of Vietnam. Acanthosaura grismeri sp. nov. differs morphologically from its congeners by a combination of the following characteristics: size moderate (SVL 77.20-91.98 mm in males, 102.70-113.09 mm in females); the absence of diastema between short nuchal and dorsal crest spines; vertebral crest includes a single row of enlarged, keeled, pointed scales; color of dorsal surface green in males and light brown and green-yellow with scattered black spots in females; the absence of black eye patch; black or brown lines from canthus rostralis-supraciliary line downward to upper eye margin, and a white band from lower eye margin downward to lip edge. Phylogenetic analyses using maximum likelihood and Bayesian inference methods for the COI gene support the monophyly of Acanthosaura grismeri sp. nov. The uncorrected pairwise distance between the new species and its congeners ranges from 7.82% to 25.36%. With the description of this species, 22 species of Acanthosaura are recognized, half of them occurring in Vietnam.
References
- Ananjeva, N.B., Ermakov, O.A., Nguyen, S.N., Nguyen, T.T., Murphy, R.W., Lukonina, S.A. & Orlov, N.L. (2020) A new species of Acanthosaura Gray, 1831 (Squamata: Agamidae) from Central Highlands, Vietnam. Russian Journal of Herpetology, 27 (4), 217–230. https://doi.org/10.30906/1026-2296-2020-27-4-217-230
- Ananjeva, N.B., Orlov, N.L. & Kalyabina-Hauf, S.A. (2008) Species of Acanthosaura Gray, 1831 (Agamidae: Sauria, Reptilia) of Vietnam: results of molecular and morphological study. Biology Bulletin, 35 (2), 178–186. https://doi.org/10.1134/S106235900802012X
- Ananjeva, N.B., Orlov, N.L., Nguyen, T.T. & Ryabov, S.A. (2011) A new species of Acanthosaura (Agamidae, Sauria) from Northwest Vietnam. Russian Journal of Herpetology, 18 (3), 195–202. https://doi.org/10.30906/1026-2296-2011-18-3-195-202
- Boulenger, G.A. (1885) Catalogue of the Lizards in the British Museum (Natural History). 2nd ed. Taylor & Francis, London, vi + 436 pp, 32 pls. https://doi.org/10.5962/bhl.title.53974
- Che, J., Chen, H., Yang, J., Jin, J., Jiang, K., Yuan, Z., Murphy, R.W. & Zhang, Y. (2012) Universal COI primers for DNA barcoding amphibians. Molecular Ecology Resources, 12 (2), 247–258. https://doi.org/10.1111/j.1755-0998.2011.03090.x
- Edgar, R.C. (2004) MUSCLE: a multiple sequence alignment method with reduced time and space complexity. BMC Bioinformatics, 5 (1), 113. https://doi.org/10.1186/1471-2105-5-113
- Felsenstein, J. (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution, 39 (4), 783–791. https://doi.org/10.1111/j.1558-5646.1985.tb00420.x
- Günther, A. (1861) Second list of Siamese reptiles. The Annals and Magazine of Natural History, Series 3, 8, 266–268.
- Hoang, D.T., Chernomor, O., von Haeseler, A., Minh, B.Q. & Vinh, L.S. (2018) UFBoot2: improving the ultrafast bootstrap approximation. Molecular Biology and Evolution, 35 (2), 518–522. https://doi.org/10.1093/molbev/msx281
- Kalyaanamoorthy, S., Minh, B.Q., Wong, T.K.F., von Haeseler, A. & Jermiin, L.S. (2017) ModelFinder: fast model selection for accurate phylogenetic estimates. Nature Methods, 14 (6), 587–589. https://doi.org/10.1038/nmeth.4285
- Liu, S., Hou, M., Mo, M. & Rao, D. (2020) A new species of the genus Acanthosaura (Squamata, Agamidae) from Yunnan, China, with comments on its conservation status. ZooKeys, 959, 113–135. https://doi.org/10.3897/zookeys.959.54601
- Liu, S. & Rao, D. (2019) A new species of the genus Acanthosaura from Yunnan, China (Squamata, Agamidae). ZooKeys, 888, 105–132. https://doi.org/10.3897/zookeys.888.38491
- Liu, S., Rao, D., Hou, M., Orlov, N.L., Ananjeva, N.B. & Zhang, D. (2022) Two new species of Acanthosaura Gray, 1831 (Reptilia: Agamidae) from Yunnan province, China. Russian Journal of Herpetology, 29 (2), 93–109. https://doi.org/10.30906/1026-2296-2022-29-2-93-109
- Ngo, N.H., Le, T.H.L., Nguyen, T.N., Nguyen, T.T., Nguyen, M.T., Phan, Q.T., Nguyen, Q.T., Ziegler, T. & Do, T.D. (2025) A new species of Acanthosaura Gray 1831 (Reptilia: Agamidae) from the Truong Son mountain range, Vietnam. European Journal of Taxonomy, 976, 108–132. https://doi.org/10.5852/ejt.2025.976.2781
- Nguyen, L.T., Do, D.T., Hoang, H.V., Nguyen, T.T., McCormack, T.E.M., Nguyen, T.Q., Orlov, N.L., Nguyen, V.D.H. & Nguyen, S.N. (2018) A new species of the genus Acanthosaura Gray, 1831 (Reptilia: Agamidae) from Central Vietnam. Russian Journal of Herpetology, 25 (4), 259–274. https://doi.org/10.30906/1026-2296-2018-25-4-259-274
- Nguyen, L.T., Schmidt, H.A., von Haeseler, A. & Minh, B.Q. (2015) IQ-TREE: a fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies. Molecular Biology and Evolution, 32 (1), 268–274. https://doi.org/10.1093/molbev/msu300
- Nguyen, S.N., Jin, J.-Q., Vo, B.D., Nguyen, L.T., Zhou, W.-W., Che, J., Murphy, R.W. & Zhang, Y.-P. (2019) A new species of Acanthosaura Gray 1831 (Reptilia: Agamidae) from Central Vietnam. Zootaxa, 4612 (4), 555–565. https://doi.org/10.11646/zootaxa.4612.4.7
- Orlov, N.L., Truong, N.Q. & Sang, N.V. (2006) A new Acanthosaura allied to A. capra GÜNTHER, 1861 from Central Vietnam and Southern Laos. Russian Journal of Herpetology, 13 (1), 61–76. https://doi.org/10.30906/1026-2296-2006-13-1-61-76
- Pauwels, O.S.G., Sumontha, M., Kunya, K., Nitikul, A., Samphanthamit, P., Wood, P.L., Jr. & Grismer, L.L. (2015) Acanthosaura phuketensis (Squamata: Agamidae), a new long-horned tree agamid from southwestern Thailand. Zootaxa, 4020 (3), 473–494. https://doi.org/10.11646/zootaxa.4020.3.4
- Ronquist, F., Teslenko, M., van der Mark, P., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology, 61 (3), 539–542. https://doi.org/10.1093/sysbio/sys029
- Simmons, J.E. (2002) Herpetological Collecting and Collections Management. Revised edition. Society for the Study of Amphibians and Reptiles. Herpetological Circular, 31, 1–153.
- Smith, M.A. (1935) The Fauna of British India, including Ceylon and Burma. Vol. II. Reptilia and Amphibia. Taylor & Francis, London, xviii + 541 pp. https://doi.org/10.5962/bhl.title.48423
- Tamura, K., Stecher, G. & Kumar, S. (2021) MEGA11: Molecular evolutionary genetics analysis version 11. Molecular Biology and Evolution, 38 (7), 3022–3027. https://doi.org/10.1093/molbev/msab120
- Taylor, E.H. (1963) The lizards of Thailand. University of Kansas Science Bulletin, 46 (5), 687–1077.
- Trivalairat, P., Kunya, K., Chanhome, L., Sumontha, M., Vasaruchapong, T., Chomngam, N. & Chiangkul, K. (2020) Acanthosaura aurantiacrista (Squamata: Agamidae), a new long horn lizard from northern Thailand. Biodiversity Data Journal, 8, e48587. https://doi.org/10.3897/BDJ.8.e48587
- Uetz, P., Freed, P., Aguilar, R., Reyes, F., Kudera, J. & Hosek, J. (2025) The Reptile Database. Available from: http://www.reptile-database.org (accessed 20 January 2025)
- Wood, P.L., Jr., Grismer, J.L., Grismer, L.L., Ahmad, N., Onn, C.K. & Bauer, A.M. (2009) Two new montane species of Acanthosaura Gray, 1831 (Squamata: Agamidae) from Peninsular Malaysia. Zootaxa, 2012 (1), 28–46. https://doi.org/10.11646/zootaxa.2012.1.2
- Wood, P.L., Jr., Grismer, L.L., Grismer, J.L., Neang, T., Chav, T. & Holden, J. (2010) A new cryptic species of Acanthosaura Gray, 1831 (Squamata: Agamidae) from Thailand and Cambodia. Zootaxa, 2488 (1), 22–38. https://doi.org/10.11646/zootaxa.2488.1.2