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Issue: Vol. 5589 No. 1: 19 Feb. 2025
Type: Article
Published: 2025-02-19
DOI: 10.11646/zootaxa.5589.1.14
Page range: 166-189
Abstract views: 81
PDF downloaded: 4

Three Novel Species of Earthworms of Genus Metaphire Sims and Easton, 1972 from Manipur, India

Department of Zoology; Dr. Harisingh Gour Vishwavidyalaya (A Central University); Sagar 470003; Madhya Pradesh; India
Department of Zoology; Govt. College Bandri; Sagar 470442; Madhya Pradesh; India
Sustainable Living; Maharishi International University; Fairfield; IA; USA
Department of Zoology; Govt. College Bandri; Sagar 470442; Madhya Pradesh; India
Department of Zoology; Dr. Harisingh Gour Vishwavidyalaya (A Central University); Sagar 470003; Madhya Pradesh; India
Annelida COI DNA barcoding Novel species Pheretimoids Phylogenetic Species Delimitation

Abstract

The systematics of pheretimoids have been hindered by limited diagnostic traits and extensive homoplasy. This study utilized an integrative taxonomic approach, combining morpho-anatomical traits and mitochondrial COI data, to delineate species and clarify phylogenetic relationships among Indian pheretimoids. Three new species of Metaphire (Megascolecidae) were identified: Metaphire manipurensis Tiwari & Yadav sp. nov., belongs to M. houlleti group with three pairs of spermathecal pores (6/7–8/9) and no post-clitellar markings; Metaphire thabiensis Tiwari & Yadav sp. nov. classified within the M. javanica group, with two pairs of spermathecal pores (7/8–8/9) and copulatory pouches lacking stalked glands and Metaphire churachandpurensis Tiwari & Yadav sp. nov., which lacks spermathecal pores but has stalked genital marking glands. Furthermore, phylogenetic analysis of COI data confirmed species placements with strong statistical support and validated species delineation through congruent OTUs. These findings advance phylogenetics and evolutionary trajectory of the group, though expanded COI datasets are needed for broader insights.

 

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